oligo dt beads  (New England Biolabs)


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    Name:
    Oligo d T 25Magnetic Beads
    Description:

    Catalog Number:
    S1419
    Price:
    307
    Category:
    Beads Racks Columns
    Applications:
    Proteomics & Glycomics
    Size:
    5 ml
    Buy from Supplier


    Structured Review

    New England Biolabs oligo dt beads
    Oligo d T 25Magnetic Beads

    https://www.bioz.com/result/oligo dt beads/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    oligo dt beads - by Bioz Stars, 2021-09
    99/100 stars

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    Related Articles

    Electrophoresis:

    Article Title: Darwin’s bark spider shares a spidroin repertoire with Caerostris extrusa but achieves extraordinary silk toughness through gene expression
    Article Snippet: .. The RNA integrity number (RIN) was estimated by electrophoresis using a TapeStation 2200 instrument with RNA ScreenTape (Agilent Technologies). mRNA was selected from the total RNA using oligo d(T). cDNA was synthesized from mRNA isolated from 100 µg of total RNA by NEBNext Oligo d(T)25 beads (skipping the Tris buffer wash step). ..

    Synthesized:

    Article Title: Darwin’s bark spider shares a spidroin repertoire with Caerostris extrusa but achieves extraordinary silk toughness through gene expression
    Article Snippet: .. The RNA integrity number (RIN) was estimated by electrophoresis using a TapeStation 2200 instrument with RNA ScreenTape (Agilent Technologies). mRNA was selected from the total RNA using oligo d(T). cDNA was synthesized from mRNA isolated from 100 µg of total RNA by NEBNext Oligo d(T)25 beads (skipping the Tris buffer wash step). ..

    Isolation:

    Article Title: Darwin’s bark spider shares a spidroin repertoire with Caerostris extrusa but achieves extraordinary silk toughness through gene expression
    Article Snippet: .. The RNA integrity number (RIN) was estimated by electrophoresis using a TapeStation 2200 instrument with RNA ScreenTape (Agilent Technologies). mRNA was selected from the total RNA using oligo d(T). cDNA was synthesized from mRNA isolated from 100 µg of total RNA by NEBNext Oligo d(T)25 beads (skipping the Tris buffer wash step). ..

    Article Title: Cap-independent translation and a precisely localized RNA sequence enable SARS-CoV-2 to control host translation and escape anti-viral response
    Article Snippet: .. mRNA library preparation for MARS-seq Total RNA was isolated from HEK293T cells using Bio Tri RNA reagent (Bio-lab) and mRNA was captured using Oligo d(T)25 magnetic Beads (NEB) according to manufacturer’s protocol. ..

    Article Title: The balance of crystalline and amorphous regions in the fibroin structure underpins the tensile strength of bagworm silk
    Article Snippet: .. Total RNA samples (100 μg) were subjected to mRNA isolation by using NEBNext Oligo d(T)25 beads (skipping the second bead washing step). ..

    Magnetic Beads:

    Article Title: Proteome-wide quantitative RNA interactome capture (qRIC) identifies phosphorylation sites with regulatory potential in RBM20
    Article Snippet: .. For the pull-down of mRNA and cross-linked mRBPs, 15 mL of oligo(dT) magnetic beads (New England Biolabs, cat# S1419S) (0.5 mL per 0.1 g of cell pellet) were pre-washed with 30 mL of lysis buffer before incubation with cell lysates for 3 hours at room temperature. ..

    Article Title: Cap-independent translation and a precisely localized RNA sequence enable SARS-CoV-2 to control host translation and escape anti-viral response
    Article Snippet: .. mRNA library preparation for MARS-seq Total RNA was isolated from HEK293T cells using Bio Tri RNA reagent (Bio-lab) and mRNA was captured using Oligo d(T)25 magnetic Beads (NEB) according to manufacturer’s protocol. ..

    Article Title: Localized and Systemic Inflammatory Mediators in a Murine Acute Mastitis Model
    Article Snippet: .. First, mRNA was enriched from 1 µg of total RNA using poly-dT oligo-attached magnetic beads and fragmented using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X). ..

    Lysis:

    Article Title: Proteome-wide quantitative RNA interactome capture (qRIC) identifies phosphorylation sites with regulatory potential in RBM20
    Article Snippet: .. For the pull-down of mRNA and cross-linked mRBPs, 15 mL of oligo(dT) magnetic beads (New England Biolabs, cat# S1419S) (0.5 mL per 0.1 g of cell pellet) were pre-washed with 30 mL of lysis buffer before incubation with cell lysates for 3 hours at room temperature. ..

    Incubation:

    Article Title: Proteome-wide quantitative RNA interactome capture (qRIC) identifies phosphorylation sites with regulatory potential in RBM20
    Article Snippet: .. For the pull-down of mRNA and cross-linked mRBPs, 15 mL of oligo(dT) magnetic beads (New England Biolabs, cat# S1419S) (0.5 mL per 0.1 g of cell pellet) were pre-washed with 30 mL of lysis buffer before incubation with cell lysates for 3 hours at room temperature. ..

    Concentration Assay:

    Article Title: Quantitative analysis of m6A RNA modification by LC-MS
    Article Snippet: .. Use 250 μg beads for 50 μg total RNA.Note: For example, the concentration of NEB #S1419S bead suspension is 5 mg/mL. ..

    Binding Assay:

    Article Title: Quantitative analysis of m6A RNA modification by LC-MS
    Article Snippet: .. Use 50 μL of NEB #S1419S bead suspension to get 250 μg beads. c.Add 0.5 mL Oligo(dT) Binding Buffer to the beads and rinse by pipetting. d.Place the tube on the magnetic rack until the solution is clear (i.e., incubate for 1 min on the magnetic rack). e. Carefully remove the supernatant using a pipette.Note: Do not use aspirator to prevent loss of the beads. f.Add 200 μL Oligo(dT) Binding Buffer to the beads and mix well. ..

    Transferring:

    Article Title: Quantitative analysis of m6A RNA modification by LC-MS
    Article Snippet: .. Use 50 μL of NEB #S1419S bead suspension to get 250 μg beads. c.Add 0.5 mL Oligo(dT) Binding Buffer to the beads and rinse by pipetting. d.Place the tube on the magnetic rack until the solution is clear (i.e., incubate for 1 min on the magnetic rack). e. Carefully remove the supernatant using a pipette.Note: Do not use aspirator to prevent loss of the beads. f.Add 200 μL Oligo(dT) Binding Buffer to the beads and mix well. ..

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    New England Biolabs oligo dt beads
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