gdna removal columns  (New England Biolabs)


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    Name:
    Monarch gDNA Removal Columns
    Description:
    Monarch gDNA Removal Columns 100 columns
    Catalog Number:
    T2017L
    Price:
    126
    Category:
    RNA Purification Kit Components
    Size:
    100 columns
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    New England Biolabs gdna removal columns
    Monarch gDNA Removal Columns
    Monarch gDNA Removal Columns 100 columns
    https://www.bioz.com/result/gdna removal columns/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gdna removal columns - by Bioz Stars, 2021-06
    94/100 stars

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    Related Articles

    Purification:

    Article Title: Transcriptional host–pathogen responses of Pseudogymnoascus destructans and three species of bats with white-nose syndrome
    Article Snippet: RNA isolation, cDNA library preparation, and RNA-sequencingTotal RNA was isolated using the animal tissue RNA purification kit (Norgen Biotek Corp., Ontario, Canada) with the following modifications to the manufacturer’s protocol. .. Genomic DNA was removed from the lysate using the gDNA removal column and the purification column from the manufacturer’s single-cell RNA purification kit was substituted to allow RNA elution in a final volume of 15 µL. .. We confirmed that all samples contained detectable amounts of intact RNA using an RNA pico 6000 chip (Agilent Technologies, California) and resolved the RNA on a 2100 Bioanalyzer (Agilent Technologies, California).

    Article Title: Hmx3a Has Essential Functions in Zebrafish Spinal Cord, Ear and Lateral Line Development
    Article Snippet: Following chloroform extraction, we added 20 µg UltraPure Glycogen (10814010; Thermo Fisher Scientific) to the aqueous phase followed by one volume of RNase-free ethanol. .. We performed RNA purification and genomic DNA removal using the Monarch Total RNA Miniprep Kit (T2010S; New England BioLabs Inc.), following manufacturer’s instructions for purifying TRIzol-extracted samples. .. RNA concentration was measured using Nanodrop 2000 (ND2000; Thermo Fisher Scientific), before synthesizing cDNA using the Bio-Rad iScript Reverse Transcription Supermix kit (170-8891; Bio-Rad).

    other:

    Article Title: Anopheles mosquitoes revealed new principles of 3D genome organization in insects
    Article Snippet: Supernatant was transferred to fresh RNAse/DNAse-free tubes and proceeded with gDNA removal columns.

    Article Title: Osteopontin/secreted phosphoprotein-1 behaves as a molecular brake regulating the neuroinflammatory response to chronic viral infection
    Article Snippet: Samples were vortexed well, and then transferred to a gDNA Removal Column.

    Article Title: Marine Oomycetes of the Genus Halophytophthora Harbor Viruses Related to Bunyaviruses
    Article Snippet: Contaminating host DNA was removed from the extracts using a combination of gDNA removal columns and DNase I treatment.

    Real-time Polymerase Chain Reaction:

    Article Title: LARP6C regulates selective mRNA translation to promote pollen tube guidance in Arabidopsis thaliana
    Article Snippet: PCR reactions were conducted with 1.5 μL (1/20th of the reverse transcription solution) of cDNA for LARP6C and 2 μL for ACTIN8 amplication, using the GoTaq G2 Flexi DNA polymerase from Promega and 30 PCR cycles. .. For qPCR analyses, total RNA was isolated using the Monarch Total RNA Miniprep Kit (New England Biolabs), and genomic DNA were eliminated by gDNA removal columns and on-column DNaseI-treated (New England Biolabs). .. Following, 5 μg of total RNA were reverse transcribed with the SuperScript IV kit (Life Technology) using an oligodT18 primer. qPCR reactions were conducted on the LightCycler 480 Multiwell Plates 384-well themocycler (Roche Applied Sciences), with the following amplification program: 5 min 95°C, 40 cycles of 15 s at 95°C, and 1 min at 60°C.

    Isolation:

    Article Title: LARP6C regulates selective mRNA translation to promote pollen tube guidance in Arabidopsis thaliana
    Article Snippet: PCR reactions were conducted with 1.5 μL (1/20th of the reverse transcription solution) of cDNA for LARP6C and 2 μL for ACTIN8 amplication, using the GoTaq G2 Flexi DNA polymerase from Promega and 30 PCR cycles. .. For qPCR analyses, total RNA was isolated using the Monarch Total RNA Miniprep Kit (New England Biolabs), and genomic DNA were eliminated by gDNA removal columns and on-column DNaseI-treated (New England Biolabs). .. Following, 5 μg of total RNA were reverse transcribed with the SuperScript IV kit (Life Technology) using an oligodT18 primer. qPCR reactions were conducted on the LightCycler 480 Multiwell Plates 384-well themocycler (Roche Applied Sciences), with the following amplification program: 5 min 95°C, 40 cycles of 15 s at 95°C, and 1 min at 60°C.

    Lysis:

    Article Title: Integrated Multi-Omic Analyses of the Genomic Modifications by Gut Microbiome-Derived Metabolites of Epicatechin, 5-(4′-Hydroxyphenyl)-γ-Valerolactone, in TNFalpha-Stimulated Primary Human Brain Microvascular Endothelial Cells
    Article Snippet: RNA Extraction Total RNA, including short RNAs as miRNAs, were extracted using Monarch® Total RNA Miniprep Kit (New England BioLabs, United States) following the manufacture’s instruction. .. Briefly, cells were lysed using a lysis buffer and genomic DNA was removed by centrifugation in gDNA removal columns. .. RNA was then fixed to the RNA purification column, a step that was followed by successive steps of washing and centrifugations.

    Centrifugation:

    Article Title: Integrated Multi-Omic Analyses of the Genomic Modifications by Gut Microbiome-Derived Metabolites of Epicatechin, 5-(4′-Hydroxyphenyl)-γ-Valerolactone, in TNFalpha-Stimulated Primary Human Brain Microvascular Endothelial Cells
    Article Snippet: RNA Extraction Total RNA, including short RNAs as miRNAs, were extracted using Monarch® Total RNA Miniprep Kit (New England BioLabs, United States) following the manufacture’s instruction. .. Briefly, cells were lysed using a lysis buffer and genomic DNA was removed by centrifugation in gDNA removal columns. .. RNA was then fixed to the RNA purification column, a step that was followed by successive steps of washing and centrifugations.

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  • 94
    New England Biolabs gdna removal columns
    Gdna Removal Columns, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gdna removal columns/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gdna removal columns - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

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