monarch dna cleanup columns  (New England Biolabs)


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    Name:
    Monarch DNA Cleanup Columns 5ug
    Description:
    Monarch DNA Cleanup Columns 5ug 100 columns
    Catalog Number:
    t1034l
    Price:
    130
    Size:
    100 columns
    Category:
    DNA Purification Cartridges Columns
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    New England Biolabs monarch dna cleanup columns
    Monarch DNA Cleanup Columns 5ug
    Monarch DNA Cleanup Columns 5ug 100 columns
    https://www.bioz.com/result/monarch dna cleanup columns/product/New England Biolabs
    Average 86 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    monarch dna cleanup columns - by Bioz Stars, 2020-01
    86/100 stars

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    DNA Extraction:

    Article Title: No longer locally extinct? Tracing the origins of a lion (Panthera leo) living in Gabon
    Article Snippet: Extraction and library amplification: skin and hair DNA extraction from skin and hair (Table ) was performed in a dedicated pre-PCR DNA laboratory at the Centre for GeoGenetics at the Natural History Museum of Denmark (Copenhagen). .. The digest was centrifuged at 6000g for 1 min, after which 500 μl of supernatant was mixed 1:8 with a binding buffer as detailed in Allentoft et al. ( ) then centrifuged through Monarch DNA Cleanup Columns (5 μg) (New England Biolabs Inc. Beverly, MA, USA).

    Article Title: Comparative performance of the BGISEQ-500 vs Illumina HiSeq2500 sequencing platforms for palaeogenomic sequencing
    Article Snippet: Paragraph title: DNA extraction ... Digests from methods B and C were centrifuged at 6000 ×G for 1 minute, after which 500 μl supernatant was mixed 1:8 with a binding buffer as detailed in Allentoft et al. [ ], then centrifuged through Monarch DNA Cleanup Columns (New England Biolabs, MA, USA).

    Nucleic Acid Electrophoresis:

    Article Title: Genomic variants identified from whole-genome resequencing of indicine cattle breeds from Pakistan
    Article Snippet: The desired size-range of all gDNA libraries were excised using gel electrophoresis, and the size-selected gDNA libraries were then extracted from the gel and purified with the help of spin column. .. After the final best fill-in step in gDNA library construction, all gDNA libraries were mixed with Qiagen PB binding buffer (1:5 volume) and cleaned by employing Monarch DNA clean-up columns (New England Biolabs, Massachusetts, USA).

    Amplification:

    Article Title: No longer locally extinct? Tracing the origins of a lion (Panthera leo) living in Gabon
    Article Snippet: Paragraph title: Extraction and library amplification: skin and hair ... The digest was centrifuged at 6000g for 1 min, after which 500 μl of supernatant was mixed 1:8 with a binding buffer as detailed in Allentoft et al. ( ) then centrifuged through Monarch DNA Cleanup Columns (5 μg) (New England Biolabs Inc. Beverly, MA, USA).

    Article Title: Genomic variants identified from whole-genome resequencing of indicine cattle breeds from Pakistan
    Article Snippet: After the final best fill-in step in gDNA library construction, all gDNA libraries were mixed with Qiagen PB binding buffer (1:5 volume) and cleaned by employing Monarch DNA clean-up columns (New England Biolabs, Massachusetts, USA). .. All libraries were subsequently amplified in 9 to 17 index PCR cycles by using common primer, the index ligated reverse primers, and BGI-forward primer.

    Ligation:

    Article Title: Genomic variants identified from whole-genome resequencing of indicine cattle breeds from Pakistan
    Article Snippet: The ligation products (gDNA libraries) were then recovered by AMPure XP Beads. .. After the final best fill-in step in gDNA library construction, all gDNA libraries were mixed with Qiagen PB binding buffer (1:5 volume) and cleaned by employing Monarch DNA clean-up columns (New England Biolabs, Massachusetts, USA).

    Isolation:

    Article Title: Polymorphisms in RAS/RAF/MEK/ERK Pathway Are Associated with Gastric Cancer
    Article Snippet: Genomic DNA was isolated via the salting out method and Proteinase K, or according to the method described by Chomczynski and Sacchi [ ]. .. In both cases, genomic DNA was further purified using Monarch PCR and DNA cleanup columns (New England Biolabs (NEB), Ipswich, MA, USA).

    Polymerase Chain Reaction:

    Article Title: Polymorphisms in RAS/RAF/MEK/ERK Pathway Are Associated with Gastric Cancer
    Article Snippet: .. In both cases, genomic DNA was further purified using Monarch PCR and DNA cleanup columns (New England Biolabs (NEB), Ipswich, MA, USA). .. Genotyping was performed using an Infinium Global Screening Array-24 BeadChip (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions, at the Human Genomics Facility (HuGe-F) in Erasmus MC, Netherlands.

    Article Title: Genomic variants identified from whole-genome resequencing of indicine cattle breeds from Pakistan
    Article Snippet: After the final best fill-in step in gDNA library construction, all gDNA libraries were mixed with Qiagen PB binding buffer (1:5 volume) and cleaned by employing Monarch DNA clean-up columns (New England Biolabs, Massachusetts, USA). .. For all libraries the number of indexed PCR cycles were determined in qPCR-quantification by using common primer, BGI-forward primer and one of the eight index ligated reverse primers (using the Agilent MX3005 qPCR machine).

    Purification:

    Article Title: Polymorphisms in RAS/RAF/MEK/ERK Pathway Are Associated with Gastric Cancer
    Article Snippet: .. In both cases, genomic DNA was further purified using Monarch PCR and DNA cleanup columns (New England Biolabs (NEB), Ipswich, MA, USA). .. Genotyping was performed using an Infinium Global Screening Array-24 BeadChip (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions, at the Human Genomics Facility (HuGe-F) in Erasmus MC, Netherlands.

    Article Title: Genomic variants identified from whole-genome resequencing of indicine cattle breeds from Pakistan
    Article Snippet: The desired size-range of all gDNA libraries were excised using gel electrophoresis, and the size-selected gDNA libraries were then extracted from the gel and purified with the help of spin column. .. After the final best fill-in step in gDNA library construction, all gDNA libraries were mixed with Qiagen PB binding buffer (1:5 volume) and cleaned by employing Monarch DNA clean-up columns (New England Biolabs, Massachusetts, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Genomic variants identified from whole-genome resequencing of indicine cattle breeds from Pakistan
    Article Snippet: After the final best fill-in step in gDNA library construction, all gDNA libraries were mixed with Qiagen PB binding buffer (1:5 volume) and cleaned by employing Monarch DNA clean-up columns (New England Biolabs, Massachusetts, USA). .. For all libraries the number of indexed PCR cycles were determined in qPCR-quantification by using common primer, BGI-forward primer and one of the eight index ligated reverse primers (using the Agilent MX3005 qPCR machine).

    Concentration Assay:

    Article Title: Comparative performance of the BGISEQ-500 vs Illumina HiSeq2500 sequencing platforms for palaeogenomic sequencing
    Article Snippet: Digests from methods B and C were centrifuged at 6000 ×G for 1 minute, after which 500 μl supernatant was mixed 1:8 with a binding buffer as detailed in Allentoft et al. [ ], then centrifuged through Monarch DNA Cleanup Columns (New England Biolabs, MA, USA). .. Prior to library construction, small aliquots of each extract were analysed on an Agilent 2200 TapeStation HS chip (Agilent Technologies, Palo Alto, CA, USA) for fragment size estimation and molar concentration.

    Incubation:

    Article Title: No longer locally extinct? Tracing the origins of a lion (Panthera leo) living in Gabon
    Article Snippet: The digest was centrifuged at 6000g for 1 min, after which 500 μl of supernatant was mixed 1:8 with a binding buffer as detailed in Allentoft et al. ( ) then centrifuged through Monarch DNA Cleanup Columns (5 μg) (New England Biolabs Inc. Beverly, MA, USA). .. DNA bound to the columns was washed with 800 μl buffer PE (Qiagen, Hilden, Germany), then eluted using two washes in 12 μl buffer EB (Qiagen)—each with an incubation of 5 min at 37 °C.

    Article Title: Comparative performance of the BGISEQ-500 vs Illumina HiSeq2500 sequencing platforms for palaeogenomic sequencing
    Article Snippet: Digests from methods B and C were centrifuged at 6000 ×G for 1 minute, after which 500 μl supernatant was mixed 1:8 with a binding buffer as detailed in Allentoft et al. [ ], then centrifuged through Monarch DNA Cleanup Columns (New England Biolabs, MA, USA). .. DNA bound to the columns was washed with 800 μl buffer PE (Qiagen), then eluted using 2 washes in 17 μl buffer EB (Qiagen)—each with an incubation for 5 minutes at 37°C.

    Article Title: Genomic variants identified from whole-genome resequencing of indicine cattle breeds from Pakistan
    Article Snippet: After the final best fill-in step in gDNA library construction, all gDNA libraries were mixed with Qiagen PB binding buffer (1:5 volume) and cleaned by employing Monarch DNA clean-up columns (New England Biolabs, Massachusetts, USA). .. For washing, size-selected gDNA libraries were first incubated at 37◦C for 5-minutes in 750 μL PE buffer (Qiagen) and finally purified gDNA libraries were eluted using 40 μL Qiagen EB.

    Selection:

    Article Title: Polymorphisms in RAS/RAF/MEK/ERK Pathway Are Associated with Gastric Cancer
    Article Snippet: Paragraph title: 2.2. Genotyping and SNP Selection ... In both cases, genomic DNA was further purified using Monarch PCR and DNA cleanup columns (New England Biolabs (NEB), Ipswich, MA, USA).

    Activity Assay:

    Article Title: Genomic variants identified from whole-genome resequencing of indicine cattle breeds from Pakistan
    Article Snippet: To reduce self-ligation, a TrueSeq end repair kit (Illumina) was utilized to generate blunt-ended gDNA fragments by a combination of exonuclease activity and fill-in reaction, prior to advance for a cleanup step using amPure xp beads (Beckman Coulter Genomics, USA). .. After the final best fill-in step in gDNA library construction, all gDNA libraries were mixed with Qiagen PB binding buffer (1:5 volume) and cleaned by employing Monarch DNA clean-up columns (New England Biolabs, Massachusetts, USA).

    Salting Out:

    Article Title: Polymorphisms in RAS/RAF/MEK/ERK Pathway Are Associated with Gastric Cancer
    Article Snippet: Genomic DNA was isolated via the salting out method and Proteinase K, or according to the method described by Chomczynski and Sacchi [ ]. .. In both cases, genomic DNA was further purified using Monarch PCR and DNA cleanup columns (New England Biolabs (NEB), Ipswich, MA, USA).

    Sequencing:

    Article Title: Genomic variants identified from whole-genome resequencing of indicine cattle breeds from Pakistan
    Article Snippet: The blunt-ended gDNA fragments were then prepared for ligation to the sequencing adaptor by adding an extra A- base to the 3′ end. .. After the final best fill-in step in gDNA library construction, all gDNA libraries were mixed with Qiagen PB binding buffer (1:5 volume) and cleaned by employing Monarch DNA clean-up columns (New England Biolabs, Massachusetts, USA).

    Binding Assay:

    Article Title: No longer locally extinct? Tracing the origins of a lion (Panthera leo) living in Gabon
    Article Snippet: .. The digest was centrifuged at 6000g for 1 min, after which 500 μl of supernatant was mixed 1:8 with a binding buffer as detailed in Allentoft et al. ( ) then centrifuged through Monarch DNA Cleanup Columns (5 μg) (New England Biolabs Inc. Beverly, MA, USA). .. DNA bound to the columns was washed with 800 μl buffer PE (Qiagen, Hilden, Germany), then eluted using two washes in 12 μl buffer EB (Qiagen)—each with an incubation of 5 min at 37 °C.

    Article Title: Comparative performance of the BGISEQ-500 vs Illumina HiSeq2500 sequencing platforms for palaeogenomic sequencing
    Article Snippet: .. Digests from methods B and C were centrifuged at 6000 ×G for 1 minute, after which 500 μl supernatant was mixed 1:8 with a binding buffer as detailed in Allentoft et al. [ ], then centrifuged through Monarch DNA Cleanup Columns (New England Biolabs, MA, USA). .. DNA bound to the columns was washed with 800 μl buffer PE (Qiagen), then eluted using 2 washes in 17 μl buffer EB (Qiagen)—each with an incubation for 5 minutes at 37°C.

    Article Title: Genomic variants identified from whole-genome resequencing of indicine cattle breeds from Pakistan
    Article Snippet: .. After the final best fill-in step in gDNA library construction, all gDNA libraries were mixed with Qiagen PB binding buffer (1:5 volume) and cleaned by employing Monarch DNA clean-up columns (New England Biolabs, Massachusetts, USA). .. For washing, size-selected gDNA libraries were first incubated at 37◦C for 5-minutes in 750 μL PE buffer (Qiagen) and finally purified gDNA libraries were eluted using 40 μL Qiagen EB.

    Chromatin Immunoprecipitation:

    Article Title: Comparative performance of the BGISEQ-500 vs Illumina HiSeq2500 sequencing platforms for palaeogenomic sequencing
    Article Snippet: Digests from methods B and C were centrifuged at 6000 ×G for 1 minute, after which 500 μl supernatant was mixed 1:8 with a binding buffer as detailed in Allentoft et al. [ ], then centrifuged through Monarch DNA Cleanup Columns (New England Biolabs, MA, USA). .. Prior to library construction, small aliquots of each extract were analysed on an Agilent 2200 TapeStation HS chip (Agilent Technologies, Palo Alto, CA, USA) for fragment size estimation and molar concentration.

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  • 86
    New England Biolabs monarch dna cleanup columns
    Monarch Dna Cleanup Columns, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch dna cleanup columns/product/New England Biolabs
    Average 86 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    monarch dna cleanup columns - by Bioz Stars, 2020-01
    86/100 stars
      Buy from Supplier

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