dna elution buffer  (New England Biolabs)


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    New England Biolabs dna elution buffer
    A tissue section on a standard aminated glass slide was lightly fixed with formaldehyde. Then, Tn5 transposition was performed at 37 °C, and the adapters containing ligation linker 1 were inserted to the cleaved genomic <t>DNA</t> at transposase accessible sites. Afterwards, a set of DNA barcode A solutions were introduced by microchannel-guided flow delivery to perform in situ ligation reaction for appending a distinct spatial barcode Ai (i = 1–50) and ligation linker 2. Then, a second set of barcodes Bj (j = 1-50) were introduced using another set of microfluidic channels perpendicularly to those in the first flow barcoding step, which were subsequently ligated at the intersections, resulting in a mosaic of tissue pixels, each containing a distinct combination of barcodes Ai and Bj (i = 1–50, j = 1–50). After DNA fragments were collected by reversing cross-linking, the library construction was completed <t>during</t> <t>PCR</t> amplification.
    Dna Elution Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna elution buffer/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna elution buffer - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Spatial profiling of chromatin accessibility in mouse and human tissues"

    Article Title: Spatial profiling of chromatin accessibility in mouse and human tissues

    Journal: Nature

    doi: 10.1038/s41586-022-05094-1

    A tissue section on a standard aminated glass slide was lightly fixed with formaldehyde. Then, Tn5 transposition was performed at 37 °C, and the adapters containing ligation linker 1 were inserted to the cleaved genomic DNA at transposase accessible sites. Afterwards, a set of DNA barcode A solutions were introduced by microchannel-guided flow delivery to perform in situ ligation reaction for appending a distinct spatial barcode Ai (i = 1–50) and ligation linker 2. Then, a second set of barcodes Bj (j = 1-50) were introduced using another set of microfluidic channels perpendicularly to those in the first flow barcoding step, which were subsequently ligated at the intersections, resulting in a mosaic of tissue pixels, each containing a distinct combination of barcodes Ai and Bj (i = 1–50, j = 1–50). After DNA fragments were collected by reversing cross-linking, the library construction was completed during PCR amplification.
    Figure Legend Snippet: A tissue section on a standard aminated glass slide was lightly fixed with formaldehyde. Then, Tn5 transposition was performed at 37 °C, and the adapters containing ligation linker 1 were inserted to the cleaved genomic DNA at transposase accessible sites. Afterwards, a set of DNA barcode A solutions were introduced by microchannel-guided flow delivery to perform in situ ligation reaction for appending a distinct spatial barcode Ai (i = 1–50) and ligation linker 2. Then, a second set of barcodes Bj (j = 1-50) were introduced using another set of microfluidic channels perpendicularly to those in the first flow barcoding step, which were subsequently ligated at the intersections, resulting in a mosaic of tissue pixels, each containing a distinct combination of barcodes Ai and Bj (i = 1–50, j = 1–50). After DNA fragments were collected by reversing cross-linking, the library construction was completed during PCR amplification.

    Techniques Used: Ligation, In Situ, Amplification

    dna elution buffer  (New England Biolabs)


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  • 94

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    New England Biolabs dna elution buffer
    A tissue section on a standard aminated glass slide was lightly fixed with formaldehyde. Then, Tn5 transposition was performed at 37 °C, and the adapters containing ligation linker 1 were inserted to the cleaved genomic <t>DNA</t> at transposase accessible sites. Afterwards, a set of DNA barcode A solutions were introduced by microchannel-guided flow delivery to perform in situ ligation reaction for appending a distinct spatial barcode Ai (i = 1–50) and ligation linker 2. Then, a second set of barcodes Bj (j = 1-50) were introduced using another set of microfluidic channels perpendicularly to those in the first flow barcoding step, which were subsequently ligated at the intersections, resulting in a mosaic of tissue pixels, each containing a distinct combination of barcodes Ai and Bj (i = 1–50, j = 1–50). After DNA fragments were collected by reversing cross-linking, the library construction was completed <t>during</t> <t>PCR</t> amplification.
    Dna Elution Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna elution buffer/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna elution buffer - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Spatial profiling of chromatin accessibility in mouse and human tissues"

    Article Title: Spatial profiling of chromatin accessibility in mouse and human tissues

    Journal: Nature

    doi: 10.1038/s41586-022-05094-1

    A tissue section on a standard aminated glass slide was lightly fixed with formaldehyde. Then, Tn5 transposition was performed at 37 °C, and the adapters containing ligation linker 1 were inserted to the cleaved genomic DNA at transposase accessible sites. Afterwards, a set of DNA barcode A solutions were introduced by microchannel-guided flow delivery to perform in situ ligation reaction for appending a distinct spatial barcode Ai (i = 1–50) and ligation linker 2. Then, a second set of barcodes Bj (j = 1-50) were introduced using another set of microfluidic channels perpendicularly to those in the first flow barcoding step, which were subsequently ligated at the intersections, resulting in a mosaic of tissue pixels, each containing a distinct combination of barcodes Ai and Bj (i = 1–50, j = 1–50). After DNA fragments were collected by reversing cross-linking, the library construction was completed during PCR amplification.
    Figure Legend Snippet: A tissue section on a standard aminated glass slide was lightly fixed with formaldehyde. Then, Tn5 transposition was performed at 37 °C, and the adapters containing ligation linker 1 were inserted to the cleaved genomic DNA at transposase accessible sites. Afterwards, a set of DNA barcode A solutions were introduced by microchannel-guided flow delivery to perform in situ ligation reaction for appending a distinct spatial barcode Ai (i = 1–50) and ligation linker 2. Then, a second set of barcodes Bj (j = 1-50) were introduced using another set of microfluidic channels perpendicularly to those in the first flow barcoding step, which were subsequently ligated at the intersections, resulting in a mosaic of tissue pixels, each containing a distinct combination of barcodes Ai and Bj (i = 1–50, j = 1–50). After DNA fragments were collected by reversing cross-linking, the library construction was completed during PCR amplification.

    Techniques Used: Ligation, In Situ, Amplification

    dna elution buffer  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
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    New England Biolabs dna elution buffer
    A tissue section on a standard aminated glass slide was lightly fixed with formaldehyde. Then, Tn5 transposition was performed at 37 °C, and the adapters containing ligation linker 1 were inserted to the cleaved genomic <t>DNA</t> at transposase accessible sites. Afterwards, a set of DNA barcode A solutions were introduced by microchannel-guided flow delivery to perform in situ ligation reaction for appending a distinct spatial barcode Ai (i = 1-50) and ligation linker 2. Then, a second set of barcodes Bj (j = 1-50) were introduced using another set of microfluidic channels perpendicularly to those in the first flow barcoding step, which were subsequently ligated at the intersections, resulting in a mosaic of tissue pixels, each containing a distinct combination of barcodes Ai and Bj (i = 1-50, j = 1-50). After DNA fragments were collected by reversing cross-linking, the library construction was completed <t>during</t> <t>PCR</t> amplification.
    Dna Elution Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna elution buffer/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna elution buffer - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Spatial-ATAC-seq: spatially resolved chromatin accessibility profiling of tissues at genome scale and cellular level"

    Article Title: Spatial-ATAC-seq: spatially resolved chromatin accessibility profiling of tissues at genome scale and cellular level

    Journal: bioRxiv

    doi: 10.1101/2021.06.06.447244

    A tissue section on a standard aminated glass slide was lightly fixed with formaldehyde. Then, Tn5 transposition was performed at 37 °C, and the adapters containing ligation linker 1 were inserted to the cleaved genomic DNA at transposase accessible sites. Afterwards, a set of DNA barcode A solutions were introduced by microchannel-guided flow delivery to perform in situ ligation reaction for appending a distinct spatial barcode Ai (i = 1-50) and ligation linker 2. Then, a second set of barcodes Bj (j = 1-50) were introduced using another set of microfluidic channels perpendicularly to those in the first flow barcoding step, which were subsequently ligated at the intersections, resulting in a mosaic of tissue pixels, each containing a distinct combination of barcodes Ai and Bj (i = 1-50, j = 1-50). After DNA fragments were collected by reversing cross-linking, the library construction was completed during PCR amplification.
    Figure Legend Snippet: A tissue section on a standard aminated glass slide was lightly fixed with formaldehyde. Then, Tn5 transposition was performed at 37 °C, and the adapters containing ligation linker 1 were inserted to the cleaved genomic DNA at transposase accessible sites. Afterwards, a set of DNA barcode A solutions were introduced by microchannel-guided flow delivery to perform in situ ligation reaction for appending a distinct spatial barcode Ai (i = 1-50) and ligation linker 2. Then, a second set of barcodes Bj (j = 1-50) were introduced using another set of microfluidic channels perpendicularly to those in the first flow barcoding step, which were subsequently ligated at the intersections, resulting in a mosaic of tissue pixels, each containing a distinct combination of barcodes Ai and Bj (i = 1-50, j = 1-50). After DNA fragments were collected by reversing cross-linking, the library construction was completed during PCR amplification.

    Techniques Used: Ligation, In Situ, Amplification


    Figure Legend Snippet:

    Techniques Used: Sequencing

    dna elution buffer  (New England Biolabs)


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    New England Biolabs dna elution buffer
    Dna Elution Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna elution buffer/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    dna elution buffer  (New England Biolabs)


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    New England Biolabs dna elution buffer
    Dna Elution Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna elution buffer/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna elution buffer - by Bioz Stars, 2023-01
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    dna elution buffer  (New England Biolabs)


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    New England Biolabs dna elution buffer
    Dna Elution Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna elution buffer/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    dna elution buffer  (New England Biolabs)


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    New England Biolabs dna elution buffer
    Dna Elution Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna elution buffer/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    dna elution buffer - by Bioz Stars, 2023-01
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    dna elution buffer  (New England Biolabs)


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    New England Biolabs dna elution buffer
    Dna Elution Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna elution buffer/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna elution buffer - by Bioz Stars, 2023-01
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    dna elution buffer  (New England Biolabs)


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    New England Biolabs dna elution buffer
    Dna Elution Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna elution buffer/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    New England Biolabs dna elution buffer
    A tissue section on a standard aminated glass slide was lightly fixed with formaldehyde. Then, Tn5 transposition was performed at 37 °C, and the adapters containing ligation linker 1 were inserted to the cleaved genomic <t>DNA</t> at transposase accessible sites. Afterwards, a set of DNA barcode A solutions were introduced by microchannel-guided flow delivery to perform in situ ligation reaction for appending a distinct spatial barcode Ai (i = 1–50) and ligation linker 2. Then, a second set of barcodes Bj (j = 1-50) were introduced using another set of microfluidic channels perpendicularly to those in the first flow barcoding step, which were subsequently ligated at the intersections, resulting in a mosaic of tissue pixels, each containing a distinct combination of barcodes Ai and Bj (i = 1–50, j = 1–50). After DNA fragments were collected by reversing cross-linking, the library construction was completed <t>during</t> <t>PCR</t> amplification.
    Dna Elution Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna elution buffer/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna elution buffer - by Bioz Stars, 2023-01
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    A tissue section on a standard aminated glass slide was lightly fixed with formaldehyde. Then, Tn5 transposition was performed at 37 °C, and the adapters containing ligation linker 1 were inserted to the cleaved genomic DNA at transposase accessible sites. Afterwards, a set of DNA barcode A solutions were introduced by microchannel-guided flow delivery to perform in situ ligation reaction for appending a distinct spatial barcode Ai (i = 1–50) and ligation linker 2. Then, a second set of barcodes Bj (j = 1-50) were introduced using another set of microfluidic channels perpendicularly to those in the first flow barcoding step, which were subsequently ligated at the intersections, resulting in a mosaic of tissue pixels, each containing a distinct combination of barcodes Ai and Bj (i = 1–50, j = 1–50). After DNA fragments were collected by reversing cross-linking, the library construction was completed during PCR amplification.

    Journal: Nature

    Article Title: Spatial profiling of chromatin accessibility in mouse and human tissues

    doi: 10.1038/s41586-022-05094-1

    Figure Lengend Snippet: A tissue section on a standard aminated glass slide was lightly fixed with formaldehyde. Then, Tn5 transposition was performed at 37 °C, and the adapters containing ligation linker 1 were inserted to the cleaved genomic DNA at transposase accessible sites. Afterwards, a set of DNA barcode A solutions were introduced by microchannel-guided flow delivery to perform in situ ligation reaction for appending a distinct spatial barcode Ai (i = 1–50) and ligation linker 2. Then, a second set of barcodes Bj (j = 1-50) were introduced using another set of microfluidic channels perpendicularly to those in the first flow barcoding step, which were subsequently ligated at the intersections, resulting in a mosaic of tissue pixels, each containing a distinct combination of barcodes Ai and Bj (i = 1–50, j = 1–50). After DNA fragments were collected by reversing cross-linking, the library construction was completed during PCR amplification.

    Article Snippet: For library construction, the lysate was first purified using the Zymo DNA Clean & Concentrator-5 kit and eluted into 20 μl of DNA elution buffer, followed by mixing with the PCR solution (2.5 µl 25 µM new P5 PCR primer, 2.5 µl 25 µM Ad2 primer, 25 µl 2× NEBNext Master Mix).

    Techniques: Ligation, In Situ, Amplification