monarch plasmid miniprep kit  (New England Biolabs)


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    Name:
    Monarch Plasmid Miniprep Kit
    Description:
    Monarch Plasmid Miniprep Kit 250 preps
    Catalog Number:
    t1010l
    Price:
    354
    Size:
    250 preps
    Category:
    Plasmid Purification Kits
    Buy from Supplier


    Structured Review

    New England Biolabs monarch plasmid miniprep kit
    Monarch Plasmid Miniprep Kit
    Monarch Plasmid Miniprep Kit 250 preps
    https://www.bioz.com/result/monarch plasmid miniprep kit/product/New England Biolabs
    Average 96 stars, based on 561 article reviews
    Price from $9.99 to $1999.99
    monarch plasmid miniprep kit - by Bioz Stars, 2020-05
    96/100 stars

    Images

    1) Product Images from "Filter paper-based spin column method for cost-efficient DNA or RNA purification"

    Article Title: Filter paper-based spin column method for cost-efficient DNA or RNA purification

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0203011

    The efficiency of filter paper for purification of nucleic acids from various sources using respective Qiagen kits. (A) Tomato genomic DNAs purified using Qiagen DNeasy plant mini kit. (B) Tomato total RNAs purified using Qiagen RNeasy plant mini kit. (C) PCR products of a GUS fragment purified using Qiagen QIAquick PCR purification kit. (D) PCR products of GUS fragment recovered from an agarose gel using a Qiagen QIAquick gel extraction kit. (E) pUC -19 plasmid DNAs purified using a Qiagen QIAprep spin miniprep kit. For each panel, from left to right are (Q) nucleic acid purified in experiments using original Qiagen spin column, (G) reassembled spin column using two layers of Whatman glass microfiber filters (Grade GF/F), and (P) reassembled spin column using two layers of Whatman qualitative filter paper, (Grade 3) respectively. Upper panel is quantification data based on three experimental replicates normalized according to performance of the Qiagen kit; lower panel is an image of agarose gel electrophoresis for the same volume of purified nucleic acids.
    Figure Legend Snippet: The efficiency of filter paper for purification of nucleic acids from various sources using respective Qiagen kits. (A) Tomato genomic DNAs purified using Qiagen DNeasy plant mini kit. (B) Tomato total RNAs purified using Qiagen RNeasy plant mini kit. (C) PCR products of a GUS fragment purified using Qiagen QIAquick PCR purification kit. (D) PCR products of GUS fragment recovered from an agarose gel using a Qiagen QIAquick gel extraction kit. (E) pUC -19 plasmid DNAs purified using a Qiagen QIAprep spin miniprep kit. For each panel, from left to right are (Q) nucleic acid purified in experiments using original Qiagen spin column, (G) reassembled spin column using two layers of Whatman glass microfiber filters (Grade GF/F), and (P) reassembled spin column using two layers of Whatman qualitative filter paper, (Grade 3) respectively. Upper panel is quantification data based on three experimental replicates normalized according to performance of the Qiagen kit; lower panel is an image of agarose gel electrophoresis for the same volume of purified nucleic acids.

    Techniques Used: Purification, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Gel Extraction, Plasmid Preparation

    2) Product Images from "Filter paper-based spin column method for cost-efficient DNA or RNA purification"

    Article Title: Filter paper-based spin column method for cost-efficient DNA or RNA purification

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0203011

    The efficiency of filter paper for purification of nucleic acids from various sources using respective Qiagen kits. (A) Tomato genomic DNAs purified using Qiagen DNeasy plant mini kit. (B) Tomato total RNAs purified using Qiagen RNeasy plant mini kit. (C) PCR products of a GUS fragment purified using Qiagen QIAquick PCR purification kit. (D) PCR products of GUS fragment recovered from an agarose gel using a Qiagen QIAquick gel extraction kit. (E) pUC -19 plasmid DNAs purified using a Qiagen QIAprep spin miniprep kit. For each panel, from left to right are (Q) nucleic acid purified in experiments using original Qiagen spin column, (G) reassembled spin column using two layers of Whatman glass microfiber filters (Grade GF/F), and (P) reassembled spin column using two layers of Whatman qualitative filter paper, (Grade 3) respectively. Upper panel is quantification data based on three experimental replicates normalized according to performance of the Qiagen kit; lower panel is an image of agarose gel electrophoresis for the same volume of purified nucleic acids.
    Figure Legend Snippet: The efficiency of filter paper for purification of nucleic acids from various sources using respective Qiagen kits. (A) Tomato genomic DNAs purified using Qiagen DNeasy plant mini kit. (B) Tomato total RNAs purified using Qiagen RNeasy plant mini kit. (C) PCR products of a GUS fragment purified using Qiagen QIAquick PCR purification kit. (D) PCR products of GUS fragment recovered from an agarose gel using a Qiagen QIAquick gel extraction kit. (E) pUC -19 plasmid DNAs purified using a Qiagen QIAprep spin miniprep kit. For each panel, from left to right are (Q) nucleic acid purified in experiments using original Qiagen spin column, (G) reassembled spin column using two layers of Whatman glass microfiber filters (Grade GF/F), and (P) reassembled spin column using two layers of Whatman qualitative filter paper, (Grade 3) respectively. Upper panel is quantification data based on three experimental replicates normalized according to performance of the Qiagen kit; lower panel is an image of agarose gel electrophoresis for the same volume of purified nucleic acids.

    Techniques Used: Purification, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Gel Extraction, Plasmid Preparation

    Related Articles

    Isolation:

    Article Title: Host cell interactions of outer membrane vesicle-associated virulence factors of enterohemorrhagic Escherichia coli O157: Intracellular delivery, trafficking and mechanisms of cell injury
    Article Snippet: .. After confirmation of the inserts´ identities and correct orientation by sequencing (Seqlab, Göttingen, Germany), plasmid DNA isolated from E . coli DH5α (Zippy Plasmid Miniprep kit, Epigenetics) was electroporated into E . coli BL21(DE3) expression host (New England Biolabs) as above. .. The cdt V-B deletion mutant (cdt V-ACΔB ) was constructed by inverse PCR using primer pair F-del-cdtB and R-del-cdtB ( ) and plasmid DNA from strain BL21(DE3)/pET23b(+)cdt V-ABC ( ) as a template.

    Purification:

    Article Title: High Nuclease Activity of Long Persisting Staphylococcus aureus Isolates Within the Airways of Cystic Fibrosis Patients Protects Against NET-Mediated Killing
    Article Snippet: .. For transformation, electro-competent cells of the CF isolate were mixed with 5 μl of the purified pCM28nuc plasmid (NEB Monarch Plasmid Miniprep Kit). .. Electroporation was executed by the Ec2 program of the BIORad MicroPulser Electroporator (Pulse 2.5 kV, number of impulse 1) in a 0.2 cm electroporation cuvette.

    Expressing:

    Article Title: Host cell interactions of outer membrane vesicle-associated virulence factors of enterohemorrhagic Escherichia coli O157: Intracellular delivery, trafficking and mechanisms of cell injury
    Article Snippet: .. After confirmation of the inserts´ identities and correct orientation by sequencing (Seqlab, Göttingen, Germany), plasmid DNA isolated from E . coli DH5α (Zippy Plasmid Miniprep kit, Epigenetics) was electroporated into E . coli BL21(DE3) expression host (New England Biolabs) as above. .. The cdt V-B deletion mutant (cdt V-ACΔB ) was constructed by inverse PCR using primer pair F-del-cdtB and R-del-cdtB ( ) and plasmid DNA from strain BL21(DE3)/pET23b(+)cdt V-ABC ( ) as a template.

    Sequencing:

    Article Title: Insights into the Active Site of Coproheme Decarboxylase from Listeria monocytogenes
    Article Snippet: .. Cells were cultivated overnight at 37 °C and plasmid DNA, carrying LmChdC M149A, Q187A, and M149A/Q187A variants, was extracted using the Monarch Plasmid Mini Prep Kit (New England BioLabs Inc.) and sent for sequencing. .. Expression and Purification of LmChdC Wild-Type and Variants WT and variants were subcloned into a modified version of the pET21(+) expression vector with an N-terminal StrepII-tag, cleavable TEV protease, or into a pETM11 expression vector with an N-terminal, TEV cleavable 6×His-tag, expressed in E. coli Tuner (DE3) cells (Merck/Novagen) and purified via a StrepTrap HP or a HisTrap HP 5 mL column (GE Healthcare).

    Article Title: Host cell interactions of outer membrane vesicle-associated virulence factors of enterohemorrhagic Escherichia coli O157: Intracellular delivery, trafficking and mechanisms of cell injury
    Article Snippet: .. After confirmation of the inserts´ identities and correct orientation by sequencing (Seqlab, Göttingen, Germany), plasmid DNA isolated from E . coli DH5α (Zippy Plasmid Miniprep kit, Epigenetics) was electroporated into E . coli BL21(DE3) expression host (New England Biolabs) as above. .. The cdt V-B deletion mutant (cdt V-ACΔB ) was constructed by inverse PCR using primer pair F-del-cdtB and R-del-cdtB ( ) and plasmid DNA from strain BL21(DE3)/pET23b(+)cdt V-ABC ( ) as a template.

    Article Title: Functional Multigenomic Screening of Human-Associated Bacteria for NF-κB-Inducing Bioactive Effectors
    Article Snippet: .. Cosmid DNA was obtained from each bioactive clone using a Monarch Plasmid Miniprep kit (NEB, Ipswich, MA) and sequenced by Sanger sequencing using primers targeting the flanking regions of the ScaI site on pJWC1 vector ( ). ..

    Transformation Assay:

    Article Title: High Nuclease Activity of Long Persisting Staphylococcus aureus Isolates Within the Airways of Cystic Fibrosis Patients Protects Against NET-Mediated Killing
    Article Snippet: .. For transformation, electro-competent cells of the CF isolate were mixed with 5 μl of the purified pCM28nuc plasmid (NEB Monarch Plasmid Miniprep Kit). .. Electroporation was executed by the Ec2 program of the BIORad MicroPulser Electroporator (Pulse 2.5 kV, number of impulse 1) in a 0.2 cm electroporation cuvette.

    Cosmid DNA:

    Article Title: Functional Multigenomic Screening of Human-Associated Bacteria for NF-κB-Inducing Bioactive Effectors
    Article Snippet: .. Cosmid DNA was obtained from each bioactive clone using a Monarch Plasmid Miniprep kit (NEB, Ipswich, MA) and sequenced by Sanger sequencing using primers targeting the flanking regions of the ScaI site on pJWC1 vector ( ). ..

    Plasmid Preparation:

    Article Title: Insights into the Active Site of Coproheme Decarboxylase from Listeria monocytogenes
    Article Snippet: .. Cells were cultivated overnight at 37 °C and plasmid DNA, carrying LmChdC M149A, Q187A, and M149A/Q187A variants, was extracted using the Monarch Plasmid Mini Prep Kit (New England BioLabs Inc.) and sent for sequencing. .. Expression and Purification of LmChdC Wild-Type and Variants WT and variants were subcloned into a modified version of the pET21(+) expression vector with an N-terminal StrepII-tag, cleavable TEV protease, or into a pETM11 expression vector with an N-terminal, TEV cleavable 6×His-tag, expressed in E. coli Tuner (DE3) cells (Merck/Novagen) and purified via a StrepTrap HP or a HisTrap HP 5 mL column (GE Healthcare).

    Article Title: Filter paper-based spin column method for cost-efficient DNA or RNA purification
    Article Snippet: .. Alternatively, spin columns with a conical (V-shape) bottom and a drip opening, such as a miniprep column from Qiagen , and a recent version adopted in NEB Monarch plasmid miniprep kit, can be recharged by reloading filter paper discs with a diameter of 5/16 inch (~8 mm) ( ). ..

    Article Title: Host cell interactions of outer membrane vesicle-associated virulence factors of enterohemorrhagic Escherichia coli O157: Intracellular delivery, trafficking and mechanisms of cell injury
    Article Snippet: .. After confirmation of the inserts´ identities and correct orientation by sequencing (Seqlab, Göttingen, Germany), plasmid DNA isolated from E . coli DH5α (Zippy Plasmid Miniprep kit, Epigenetics) was electroporated into E . coli BL21(DE3) expression host (New England Biolabs) as above. .. The cdt V-B deletion mutant (cdt V-ACΔB ) was constructed by inverse PCR using primer pair F-del-cdtB and R-del-cdtB ( ) and plasmid DNA from strain BL21(DE3)/pET23b(+)cdt V-ABC ( ) as a template.

    Article Title: Functional Multigenomic Screening of Human-Associated Bacteria for NF-κB-Inducing Bioactive Effectors
    Article Snippet: .. Cosmid DNA was obtained from each bioactive clone using a Monarch Plasmid Miniprep kit (NEB, Ipswich, MA) and sequenced by Sanger sequencing using primers targeting the flanking regions of the ScaI site on pJWC1 vector ( ). ..

    Article Title: TET enzymes control antibody production and shape the mutational landscape in germinal centre B cells
    Article Snippet: .. Plasmid DNA was extracted using the Monarch Plasmid Miniprep Kit (New England BioLabs, T1010L) as per manufacturer's instructions. .. Subsequently, Sanger sequencing was performed using the pJet1.2 F primer detailed above.

    Article Title: High Nuclease Activity of Long Persisting Staphylococcus aureus Isolates Within the Airways of Cystic Fibrosis Patients Protects Against NET-Mediated Killing
    Article Snippet: .. For transformation, electro-competent cells of the CF isolate were mixed with 5 μl of the purified pCM28nuc plasmid (NEB Monarch Plasmid Miniprep Kit). .. Electroporation was executed by the Ec2 program of the BIORad MicroPulser Electroporator (Pulse 2.5 kV, number of impulse 1) in a 0.2 cm electroporation cuvette.

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  • About
  • News
  • Press Release
  • Team
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  • Contact
  • Bioz Stars
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  • 96
    New England Biolabs monarch plasmid miniprep kit
    The efficiency of filter paper for purification of nucleic acids from various sources using respective Qiagen kits. (A) Tomato genomic DNAs purified using Qiagen DNeasy plant mini kit. (B) Tomato total RNAs purified using Qiagen RNeasy plant mini kit. (C) PCR products of a GUS fragment purified using Qiagen QIAquick PCR purification kit. (D) PCR products of GUS fragment recovered from an agarose gel using a Qiagen QIAquick gel extraction kit. (E) pUC -19 plasmid DNAs purified using a Qiagen QIAprep spin <t>miniprep</t> kit. For each panel, from left to right are (Q) nucleic acid purified in experiments using original Qiagen spin column, (G) reassembled spin column using two layers of Whatman glass microfiber filters (Grade GF/F), and (P) reassembled spin column using two layers of Whatman qualitative filter paper, (Grade 3) respectively. Upper panel is quantification data based on three experimental replicates normalized according to performance of the Qiagen kit; lower panel is an image of agarose gel electrophoresis for the same volume of purified nucleic acids.
    Monarch Plasmid Miniprep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch plasmid miniprep kit/product/New England Biolabs
    Average 96 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    monarch plasmid miniprep kit - by Bioz Stars, 2020-05
    96/100 stars
      Buy from Supplier

    Image Search Results


    The efficiency of filter paper for purification of nucleic acids from various sources using respective Qiagen kits. (A) Tomato genomic DNAs purified using Qiagen DNeasy plant mini kit. (B) Tomato total RNAs purified using Qiagen RNeasy plant mini kit. (C) PCR products of a GUS fragment purified using Qiagen QIAquick PCR purification kit. (D) PCR products of GUS fragment recovered from an agarose gel using a Qiagen QIAquick gel extraction kit. (E) pUC -19 plasmid DNAs purified using a Qiagen QIAprep spin miniprep kit. For each panel, from left to right are (Q) nucleic acid purified in experiments using original Qiagen spin column, (G) reassembled spin column using two layers of Whatman glass microfiber filters (Grade GF/F), and (P) reassembled spin column using two layers of Whatman qualitative filter paper, (Grade 3) respectively. Upper panel is quantification data based on three experimental replicates normalized according to performance of the Qiagen kit; lower panel is an image of agarose gel electrophoresis for the same volume of purified nucleic acids.

    Journal: PLoS ONE

    Article Title: Filter paper-based spin column method for cost-efficient DNA or RNA purification

    doi: 10.1371/journal.pone.0203011

    Figure Lengend Snippet: The efficiency of filter paper for purification of nucleic acids from various sources using respective Qiagen kits. (A) Tomato genomic DNAs purified using Qiagen DNeasy plant mini kit. (B) Tomato total RNAs purified using Qiagen RNeasy plant mini kit. (C) PCR products of a GUS fragment purified using Qiagen QIAquick PCR purification kit. (D) PCR products of GUS fragment recovered from an agarose gel using a Qiagen QIAquick gel extraction kit. (E) pUC -19 plasmid DNAs purified using a Qiagen QIAprep spin miniprep kit. For each panel, from left to right are (Q) nucleic acid purified in experiments using original Qiagen spin column, (G) reassembled spin column using two layers of Whatman glass microfiber filters (Grade GF/F), and (P) reassembled spin column using two layers of Whatman qualitative filter paper, (Grade 3) respectively. Upper panel is quantification data based on three experimental replicates normalized according to performance of the Qiagen kit; lower panel is an image of agarose gel electrophoresis for the same volume of purified nucleic acids.

    Article Snippet: Alternatively, spin columns with a conical (V-shape) bottom and a drip opening, such as a miniprep column from Qiagen , and a recent version adopted in NEB Monarch plasmid miniprep kit, can be recharged by reloading filter paper discs with a diameter of 5/16 inch (~8 mm) ( ).

    Techniques: Purification, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Gel Extraction, Plasmid Preparation