dsdna shearase plus  (Zymo Research)


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    Name:
    dsDNA Shearase Plus
    Description:
    dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5 phosphate and 3 hydroxyl termini It has a particularly strong preference for dsDNA and generates random ended DNA fragments of the desired size in a single step This enzyme is compatible with low volume inputs thus minimizing sample loss
    Catalog Number:
    e2018-50
    Price:
    None
    Applications:
    DNA Fragmentation
    Size:
    50 units
    Category:
    Life Science Reagents and Media
    Buy from Supplier


    Structured Review

    Zymo Research dsdna shearase plus
    dsDNA Shearase Plus
    dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5 phosphate and 3 hydroxyl termini It has a particularly strong preference for dsDNA and generates random ended DNA fragments of the desired size in a single step This enzyme is compatible with low volume inputs thus minimizing sample loss
    https://www.bioz.com/result/dsdna shearase plus/product/Zymo Research
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    dsdna shearase plus - by Bioz Stars, 2020-02
    93/100 stars

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    Related Articles

    Amplification:

    Article Title: Humanin Prevents Age-Related Cognitive Decline in Mice and is Associated with Improved Cognitive Age in Humans
    Article Snippet: The PCR products were run on a gel to check for correct amplification and then excised and purified using the QIAquick gel extraction kit (Qiagen). .. Illumina sequencing libraries were prepared in a 96-well plate format according to Dunham & Friesen (2013) with the exception that DNA was sheared with dsDNA Shearase Plus (Zymo: Irvine, CA, USA) and cleaned using Agencourt AMPure XP beads (Beckman- Coulter: Indianapolis, IN, USA).

    Cytometry:

    Article Title: Energy and carbon metabolisms in a deep terrestrial subsurface fluid microbial community
    Article Snippet: Sequencing was performed at the University of Southern California’s Genome and Cytometry Core Facility (Los Angeles, CA, USA). .. Illumina sequencing libraries were prepared according to with the exception that DNA was sheared with dsDNA Shearase Plus (Zymo: Irvine, CA, USA) and cleaned using Agencourt AMPure XP beads (Beckman-Coulter: Indianapolis, IN, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Energy and carbon metabolisms in a deep terrestrial subsurface fluid microbial community
    Article Snippet: Illumina sequencing libraries were prepared according to with the exception that DNA was sheared with dsDNA Shearase Plus (Zymo: Irvine, CA, USA) and cleaned using Agencourt AMPure XP beads (Beckman-Coulter: Indianapolis, IN, USA). .. The libraries were then pooled in equimolar concentrations, quantified via qPCR using the Kapa Biosystems Library Quantification Kit and paired-end sequenced on an Illumina HiSeq 2500 platform.

    Article Title: Humanin Prevents Age-Related Cognitive Decline in Mice and is Associated with Improved Cognitive Age in Humans
    Article Snippet: Illumina sequencing libraries were prepared in a 96-well plate format according to Dunham & Friesen (2013) with the exception that DNA was sheared with dsDNA Shearase Plus (Zymo: Irvine, CA, USA) and cleaned using Agencourt AMPure XP beads (Beckman- Coulter: Indianapolis, IN, USA). .. The libraries were then pooled equimolarly, and the final pool was quantified via qPCR using the Kapa Biosystems Library Quantification Kit, according to manufacturer’s instructions.

    Incubation:

    Article Title: Isolation and genome-wide characterization of cellular DNA:RNA triplex structures
    Article Snippet: Finally, samples were incubated for 5 min at 37°C with RNase I (3.125 mU/μl, Thermo Fisher Scientific) to yield RNA with an average size of 100–150 nucleotides. .. For Solid Phase Reversible Immobilization (SPRI)-based paramagnetic bead size selection, 16–20 μg of nucleic acids were treated for 30 min with 200 mU/μl RNase H and 12.5 mU/μl Shearase Plus (Zymo Research) to digest RNAs in R-loops and trim the size of genomic DNA to 5–10 kb.

    Genome Wide:

    Article Title: Isolation and genome-wide characterization of cellular DNA:RNA triplex structures
    Article Snippet: Paragraph title: Genome-wide isolation of DNA-associated RNA ... For Solid Phase Reversible Immobilization (SPRI)-based paramagnetic bead size selection, 16–20 μg of nucleic acids were treated for 30 min with 200 mU/μl RNase H and 12.5 mU/μl Shearase Plus (Zymo Research) to digest RNAs in R-loops and trim the size of genomic DNA to 5–10 kb.

    Modification:

    Article Title: Energy and carbon metabolisms in a deep terrestrial subsurface fluid microbial community
    Article Snippet: Whole genomic DNA was extracted using a modified phenol–chloroform method with ethanol precipitation as previously described in . .. Illumina sequencing libraries were prepared according to with the exception that DNA was sheared with dsDNA Shearase Plus (Zymo: Irvine, CA, USA) and cleaned using Agencourt AMPure XP beads (Beckman-Coulter: Indianapolis, IN, USA).

    other:

    Article Title: MULTIPLEXED ELIMINATION OF WILD-TYPE DNA AND HIGH RESOLUTION MELTING PRIOR TO TARGETED RESEQUENCING OF LIQUID BIOPSIES
    Article Snippet: Genomic DNA shearing was performed with the use of dsDNA Shearase Plus (Zymo Research) in a total 10μl reaction (1x dsDNA Shearase Plus Reaction Buffer, 100ng of genomic DNA and 1 unit of dsDNA Shearase Plus enzyme).

    Sequencing:

    Article Title: Energy and carbon metabolisms in a deep terrestrial subsurface fluid microbial community
    Article Snippet: .. Illumina sequencing libraries were prepared according to with the exception that DNA was sheared with dsDNA Shearase Plus (Zymo: Irvine, CA, USA) and cleaned using Agencourt AMPure XP beads (Beckman-Coulter: Indianapolis, IN, USA). .. Fragment size selection was also carried out using beads instead of gel electrophoresis.

    Article Title: Humanin Prevents Age-Related Cognitive Decline in Mice and is Associated with Improved Cognitive Age in Humans
    Article Snippet: .. Illumina sequencing libraries were prepared in a 96-well plate format according to Dunham & Friesen (2013) with the exception that DNA was sheared with dsDNA Shearase Plus (Zymo: Irvine, CA, USA) and cleaned using Agencourt AMPure XP beads (Beckman- Coulter: Indianapolis, IN, USA). ..

    Binding Assay:

    Article Title: Humanin Prevents Age-Related Cognitive Decline in Mice and is Associated with Improved Cognitive Age in Humans
    Article Snippet: In short, the buffy coats were lysed using proteinase K and buffer added to optimize the DNA binding conditions. .. Illumina sequencing libraries were prepared in a 96-well plate format according to Dunham & Friesen (2013) with the exception that DNA was sheared with dsDNA Shearase Plus (Zymo: Irvine, CA, USA) and cleaned using Agencourt AMPure XP beads (Beckman- Coulter: Indianapolis, IN, USA).

    DNA Extraction:

    Article Title: Energy and carbon metabolisms in a deep terrestrial subsurface fluid microbial community
    Article Snippet: Paragraph title: DNA extraction and sequencing ... Illumina sequencing libraries were prepared according to with the exception that DNA was sheared with dsDNA Shearase Plus (Zymo: Irvine, CA, USA) and cleaned using Agencourt AMPure XP beads (Beckman-Coulter: Indianapolis, IN, USA).

    Article Title: Humanin Prevents Age-Related Cognitive Decline in Mice and is Associated with Improved Cognitive Age in Humans
    Article Snippet: Paragraph title: DNA extraction and next generation sequencing ... Illumina sequencing libraries were prepared in a 96-well plate format according to Dunham & Friesen (2013) with the exception that DNA was sheared with dsDNA Shearase Plus (Zymo: Irvine, CA, USA) and cleaned using Agencourt AMPure XP beads (Beckman- Coulter: Indianapolis, IN, USA).

    Nucleic Acid Electrophoresis:

    Article Title: Energy and carbon metabolisms in a deep terrestrial subsurface fluid microbial community
    Article Snippet: Illumina sequencing libraries were prepared according to with the exception that DNA was sheared with dsDNA Shearase Plus (Zymo: Irvine, CA, USA) and cleaned using Agencourt AMPure XP beads (Beckman-Coulter: Indianapolis, IN, USA). .. Fragment size selection was also carried out using beads instead of gel electrophoresis.

    Mutagenesis:

    Article Title: Elimination of unaltered DNA in mixed clinical samples via nuclease-assisted minor-allele enrichment
    Article Snippet: NaME-PrO assay Genomic DNA was treated with dsDNA Shearase Plus (ZYMO Research, CA, USA) to generate random fragments in the size range 100–500 bp. .. For the lowest 0.03 and 0.01% mutation abundance serial mutation dilution studies, 100 ng genomic DNA was used as starting material.

    Isolation:

    Article Title: Isolation and genome-wide characterization of cellular DNA:RNA triplex structures
    Article Snippet: Paragraph title: Genome-wide isolation of DNA-associated RNA ... For Solid Phase Reversible Immobilization (SPRI)-based paramagnetic bead size selection, 16–20 μg of nucleic acids were treated for 30 min with 200 mU/μl RNase H and 12.5 mU/μl Shearase Plus (Zymo Research) to digest RNAs in R-loops and trim the size of genomic DNA to 5–10 kb.

    Electrophoretic Mobility Shift Assay:

    Article Title: Isolation and genome-wide characterization of cellular DNA:RNA triplex structures
    Article Snippet: To determine the concentration of RNase H required to digest cellular R-loops, radiolabeled synthetic heteroduplexes were spiked into the samples in the presence increasing amounts of RNase H and digestion was monitored by electrophoretic mobility shift assay (EMSA) ( ). .. For Solid Phase Reversible Immobilization (SPRI)-based paramagnetic bead size selection, 16–20 μg of nucleic acids were treated for 30 min with 200 mU/μl RNase H and 12.5 mU/μl Shearase Plus (Zymo Research) to digest RNAs in R-loops and trim the size of genomic DNA to 5–10 kb.

    Purification:

    Article Title: Humanin Prevents Age-Related Cognitive Decline in Mice and is Associated with Improved Cognitive Age in Humans
    Article Snippet: The PCR products were run on a gel to check for correct amplification and then excised and purified using the QIAquick gel extraction kit (Qiagen). .. Illumina sequencing libraries were prepared in a 96-well plate format according to Dunham & Friesen (2013) with the exception that DNA was sheared with dsDNA Shearase Plus (Zymo: Irvine, CA, USA) and cleaned using Agencourt AMPure XP beads (Beckman- Coulter: Indianapolis, IN, USA).

    Polymerase Chain Reaction:

    Article Title: Humanin Prevents Age-Related Cognitive Decline in Mice and is Associated with Improved Cognitive Age in Humans
    Article Snippet: The PCR products were run on a gel to check for correct amplification and then excised and purified using the QIAquick gel extraction kit (Qiagen). .. Illumina sequencing libraries were prepared in a 96-well plate format according to Dunham & Friesen (2013) with the exception that DNA was sheared with dsDNA Shearase Plus (Zymo: Irvine, CA, USA) and cleaned using Agencourt AMPure XP beads (Beckman- Coulter: Indianapolis, IN, USA).

    Immu-Puri:

    Article Title: Isolation and genome-wide characterization of cellular DNA:RNA triplex structures
    Article Snippet: .. To separate free RNA from DNA-associated RNA by immunopurification with anti-DNA antibody, 16–20 μg of nucleic acids were treated for 30 min with 200 mU/μl RNase H and 75 mU/μl Shearase Plus to digest RNAs in R-loops and trim the size of genomic DNA to 500–1000 bp. .. After partial digestion with RNase I (3.125 mU/μl, 37°C), 2 μg of nucleic acids were incubated with 6 μg of immobilized anti-dsDNA antibody (Abcam ab27156) in 200 μl IP buffer (10 mM Tris–HCl [pH 7.4], 50 mM KCl, 5 mM MgCl2 , 0.05% Tween-20, 20 U RNasin (Promega), 40 U SUPERase In RNase inhibitor (Invitrogen)) for 2 h at room temperature.

    Article Title: Isolation and genome-wide characterization of cellular DNA:RNA triplex structures
    Article Snippet: For Solid Phase Reversible Immobilization (SPRI)-based paramagnetic bead size selection, 16–20 μg of nucleic acids were treated for 30 min with 200 mU/μl RNase H and 12.5 mU/μl Shearase Plus (Zymo Research) to digest RNAs in R-loops and trim the size of genomic DNA to 5–10 kb. .. To separate free RNA from DNA-associated RNA by immunopurification with anti-DNA antibody, 16–20 μg of nucleic acids were treated for 30 min with 200 mU/μl RNase H and 75 mU/μl Shearase Plus to digest RNAs in R-loops and trim the size of genomic DNA to 500–1000 bp.

    Selection:

    Article Title: Isolation and genome-wide characterization of cellular DNA:RNA triplex structures
    Article Snippet: .. For Solid Phase Reversible Immobilization (SPRI)-based paramagnetic bead size selection, 16–20 μg of nucleic acids were treated for 30 min with 200 mU/μl RNase H and 12.5 mU/μl Shearase Plus (Zymo Research) to digest RNAs in R-loops and trim the size of genomic DNA to 5–10 kb. .. RNA was partially digested with RNase I (3.125 U/μl at 37°C) before size selection using 0.4 sample volumes of AMPure XP beads (Backman Coulter).

    Article Title: Energy and carbon metabolisms in a deep terrestrial subsurface fluid microbial community
    Article Snippet: Illumina sequencing libraries were prepared according to with the exception that DNA was sheared with dsDNA Shearase Plus (Zymo: Irvine, CA, USA) and cleaned using Agencourt AMPure XP beads (Beckman-Coulter: Indianapolis, IN, USA). .. Fragment size selection was also carried out using beads instead of gel electrophoresis.

    Ethanol Precipitation:

    Article Title: Energy and carbon metabolisms in a deep terrestrial subsurface fluid microbial community
    Article Snippet: Whole genomic DNA was extracted using a modified phenol–chloroform method with ethanol precipitation as previously described in . .. Illumina sequencing libraries were prepared according to with the exception that DNA was sheared with dsDNA Shearase Plus (Zymo: Irvine, CA, USA) and cleaned using Agencourt AMPure XP beads (Beckman-Coulter: Indianapolis, IN, USA).

    Next-Generation Sequencing:

    Article Title: Humanin Prevents Age-Related Cognitive Decline in Mice and is Associated with Improved Cognitive Age in Humans
    Article Snippet: Paragraph title: DNA extraction and next generation sequencing ... Illumina sequencing libraries were prepared in a 96-well plate format according to Dunham & Friesen (2013) with the exception that DNA was sheared with dsDNA Shearase Plus (Zymo: Irvine, CA, USA) and cleaned using Agencourt AMPure XP beads (Beckman- Coulter: Indianapolis, IN, USA).

    Spectrophotometry:

    Article Title: Energy and carbon metabolisms in a deep terrestrial subsurface fluid microbial community
    Article Snippet: DNA concentration was checked on a Qubit 2.0 fluorometer (Thermo Fisher Scientific, Chino, CA, USA), and purity was measured on a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific) before samples were sent for sequencing. .. Illumina sequencing libraries were prepared according to with the exception that DNA was sheared with dsDNA Shearase Plus (Zymo: Irvine, CA, USA) and cleaned using Agencourt AMPure XP beads (Beckman-Coulter: Indianapolis, IN, USA).

    Concentration Assay:

    Article Title: Isolation and genome-wide characterization of cellular DNA:RNA triplex structures
    Article Snippet: To determine the concentration of RNase H required to digest cellular R-loops, radiolabeled synthetic heteroduplexes were spiked into the samples in the presence increasing amounts of RNase H and digestion was monitored by electrophoretic mobility shift assay (EMSA) ( ). .. For Solid Phase Reversible Immobilization (SPRI)-based paramagnetic bead size selection, 16–20 μg of nucleic acids were treated for 30 min with 200 mU/μl RNase H and 12.5 mU/μl Shearase Plus (Zymo Research) to digest RNAs in R-loops and trim the size of genomic DNA to 5–10 kb.

    Article Title: Energy and carbon metabolisms in a deep terrestrial subsurface fluid microbial community
    Article Snippet: DNA concentration was checked on a Qubit 2.0 fluorometer (Thermo Fisher Scientific, Chino, CA, USA), and purity was measured on a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific) before samples were sent for sequencing. .. Illumina sequencing libraries were prepared according to with the exception that DNA was sheared with dsDNA Shearase Plus (Zymo: Irvine, CA, USA) and cleaned using Agencourt AMPure XP beads (Beckman-Coulter: Indianapolis, IN, USA).

    Gel Extraction:

    Article Title: Humanin Prevents Age-Related Cognitive Decline in Mice and is Associated with Improved Cognitive Age in Humans
    Article Snippet: The PCR products were run on a gel to check for correct amplification and then excised and purified using the QIAquick gel extraction kit (Qiagen). .. Illumina sequencing libraries were prepared in a 96-well plate format according to Dunham & Friesen (2013) with the exception that DNA was sheared with dsDNA Shearase Plus (Zymo: Irvine, CA, USA) and cleaned using Agencourt AMPure XP beads (Beckman- Coulter: Indianapolis, IN, USA).

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  • 95
    Zymo Research dsdna shearase
    Dsdna Shearase, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dsdna shearase/product/Zymo Research
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    dsdna shearase - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    93
    Zymo Research dsdna shearase plus
    Dsdna Shearase Plus, supplied by Zymo Research, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dsdna shearase plus/product/Zymo Research
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dsdna shearase plus - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

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