dsdna shearase  (Zymo Research)


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    Name:
    dsDNA Shearase Plus
    Description:
    dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5 phosphate and 3 hydroxyl termini It has a particularly strong preference for dsDNA and generates random ended DNA fragments of the desired size in a single step This enzyme is compatible with low volume inputs thus minimizing sample loss
    Catalog Number:
    e2018-50
    Price:
    None
    Applications:
    DNA Fragmentation
    Size:
    50 units
    Category:
    Life Science Reagents and Media
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    Structured Review

    Zymo Research dsdna shearase
    dsDNA Shearase Plus
    dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5 phosphate and 3 hydroxyl termini It has a particularly strong preference for dsDNA and generates random ended DNA fragments of the desired size in a single step This enzyme is compatible with low volume inputs thus minimizing sample loss
    https://www.bioz.com/result/dsdna shearase/product/Zymo Research
    Average 95 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    dsdna shearase - by Bioz Stars, 2020-10
    95/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: The genomic landscape of meiotic crossovers and gene conversions in Arabidopsis thaliana
    Article Snippet: .. Libraries for the eight shallowly sequenced tetrads were prepared by fragmenting the DNA using dsDNA Shearase (Zymo Research, Irvine, CA, USA) to obtain 100- to 1000-bp fragments, A-tailing using Klenow exonuclease (New England Biolabs, Ipswitch, MA, USA), ligating to indexed adapters using the QuickLigation kit (New England Biolabs, Ipswitch, MA, USA), size selection to 300 to 500 bp using AMPure XP SPRI beads (Beckman-Coulter, Pasadena, CA), and PCR enrichment using the Phusion DNA polymerase (New England Biolabs, Ipswitch, MA, USA). ..

    Sequencing:

    Article Title: RRHP: a tag-based approach for 5-hydroxymethylcytosine mapping at single-site resolution
    Article Snippet: .. JBP-1-mediated enrichment sequencing library preparation and analysis The enrichment sequencing libraries were prepared from 1 ug gDNA fragmented with dsDNA Shearase (Zymo Research). .. Fragments were then A-tailed with Klenow exo- fragment (NEB) and ligated to adapters per standard Illumina library preparation protocols.

    Selection:

    Article Title: The genomic landscape of meiotic crossovers and gene conversions in Arabidopsis thaliana
    Article Snippet: .. Libraries for the eight shallowly sequenced tetrads were prepared by fragmenting the DNA using dsDNA Shearase (Zymo Research, Irvine, CA, USA) to obtain 100- to 1000-bp fragments, A-tailing using Klenow exonuclease (New England Biolabs, Ipswitch, MA, USA), ligating to indexed adapters using the QuickLigation kit (New England Biolabs, Ipswitch, MA, USA), size selection to 300 to 500 bp using AMPure XP SPRI beads (Beckman-Coulter, Pasadena, CA), and PCR enrichment using the Phusion DNA polymerase (New England Biolabs, Ipswitch, MA, USA). ..

    other:

    Article Title: Rapid and Inexpensive Whole-Genome Genotyping-by-Sequencing for Crossover Localization and Fine-Scale Genetic Mapping
    Article Snippet: To test whether fragmentation by dsDNA Shearase introduces a biased representation of the genome and to generate markers for the Ws-2 parent of our mapping populations, we scaled up the same protocol to produce libraries for Ws-2 whole genome resequencing to an average depth of about 25x.

    Article Title: MULTIPLEXED ELIMINATION OF WILD-TYPE DNA AND HIGH RESOLUTION MELTING PRIOR TO TARGETED RESEQUENCING OF LIQUID BIOPSIES
    Article Snippet: Genomic DNA shearing was performed with the use of dsDNA Shearase Plus (Zymo Research) in a total 10μl reaction (1x dsDNA Shearase Plus Reaction Buffer, 100ng of genomic DNA and 1 unit of dsDNA Shearase Plus enzyme).

    Purification:

    Article Title: Evidence for Epigenetic Regulation of Pro-inflammatory Cytokines, Interleukin-12 and Interferon Gamma, in Peripheral Blood Mononuclear Cells from PTSD patients
    Article Snippet: .. Purified genomic DNA was treated with dsDNA Shearase (Zymo Research, Irvine, CA). .. DNA fragments with size from 200bp to 400 bp were purified to construct sequencing library using Illumina Chip-seq library preparation kit. dsDNA was then denatured and immunoprecipitated with anti 5-methylcytosine antibody using a MeDIP kit from Diagenode (Denville, NJ).

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  • 95
    Zymo Research dsdna shearase
    Dsdna Shearase, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dsdna shearase/product/Zymo Research
    Average 95 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    dsdna shearase - by Bioz Stars, 2020-10
    95/100 stars
      Buy from Supplier

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