elisa  (Sino Biological)


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    Name:
    Goat Anti Human IgG Fc Secondary Antibody
    Description:

    Catalog Number:
    SSA001
    Price:
    None
    Category:
    Secondary Antibody
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    Structured Review

    Sino Biological elisa

    https://www.bioz.com/result/elisa/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisa - by Bioz Stars, 2021-06
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Integrating SpyCatcher/SpyTag covalent fusion technology into phage display workflows for rapid antibody discovery
    Article Snippet: Appropriate dilutions of mCD47-SpyC and mSIRPα-Fc that yielded signals within the linear part of the response curve were determined previously by chequerboard ELISA titration. .. Following incubation for 1 h, the bound mSIRPα-Fc levels were determined by colorimetric detection with goat anti-human IgG Fc polyclonal HRP conjugate (Sino Biologicals, #SSA001; 1:5000 dilution) using TMB as substrate. .. Neutralizing scFv clones were reformatted as murine IgG2a and purified.

    Article Title: TCRγ4δ1-Engineered αβT Cells Exhibit Effective Antitumor Activity
    Article Snippet: Immunofluorescence was measured with an Accuri 6 Flow cytometer and analyzed by BD Accuri C6 software. .. TCRγ4δ1-Fc was constructed by fusing the complete extracellular domain of TCRγ4δ1 to the constant region of human IgG1 ( ) and expressed by Sino Biological Inc. For flow cytometry: To examine the binding of TCRγ4δ1-Fc with various cells, including HepG2, BGC-803, K562, Raji, PBMCs, ccc-HEL-1, 1308.1.86 and 293T cells, the tested cells were individually incubated with TCRγ4δ1-Fc for 60 min at 4°C. .. FITC-conjugated goat anti-human IgG antibody (ZSGB-bio, China) was added and incubated for 30 min at 4°C; human IgG-Fc was used as a control.

    Article Title: Quantitative evaluation of protective antibody response induced by hepatitis E vaccine in humans
    Article Snippet: Briefly, the recombinant p239(1) proteins with or without boiling were loaded onto SDS-PAGE gel and subsequently electroblotted onto nitrocellulose membrane at 35 mA for 55 min. .. The membranes were blocked with 5% skim milk and then incubated with sera or p239(1)-specific mAbs for 30 min. After rinse, the membranes were incubated with anti-human HRP-conjugated antibody (Sino Biological inc, Cat# SSA001). ..

    Article Title: Integrating SpyCatcher/SpyTag covalent fusion technology into phage display workflows for rapid antibody discovery
    Article Snippet: All competitors were titrated in a 3-fold dilution series starting at 2.5 μg/ml and mixed 1:1 v/v (200 μl final) with in-house produced mSIRPα-Fc HEK supernatant before addition to the plate. .. After 1 h of incubation, binding of mSIRPα-Fc to plate-bound mCD47 was revealed by goat anti-human IgG HRP-conjugated antibody (Sino Biologicals, #SSA001; 1:5000 dilution) followed by the addition of TMB substrate for colorimetric detection. .. Surface plasmon resonance (SPR)SPR analysis was performed on a Biacore T200 instrument (GE Healthcare).

    Construct:

    Article Title: TCRγ4δ1-Engineered αβT Cells Exhibit Effective Antitumor Activity
    Article Snippet: Immunofluorescence was measured with an Accuri 6 Flow cytometer and analyzed by BD Accuri C6 software. .. TCRγ4δ1-Fc was constructed by fusing the complete extracellular domain of TCRγ4δ1 to the constant region of human IgG1 ( ) and expressed by Sino Biological Inc. For flow cytometry: To examine the binding of TCRγ4δ1-Fc with various cells, including HepG2, BGC-803, K562, Raji, PBMCs, ccc-HEL-1, 1308.1.86 and 293T cells, the tested cells were individually incubated with TCRγ4δ1-Fc for 60 min at 4°C. .. FITC-conjugated goat anti-human IgG antibody (ZSGB-bio, China) was added and incubated for 30 min at 4°C; human IgG-Fc was used as a control.

    Flow Cytometry:

    Article Title: TCRγ4δ1-Engineered αβT Cells Exhibit Effective Antitumor Activity
    Article Snippet: Immunofluorescence was measured with an Accuri 6 Flow cytometer and analyzed by BD Accuri C6 software. .. TCRγ4δ1-Fc was constructed by fusing the complete extracellular domain of TCRγ4δ1 to the constant region of human IgG1 ( ) and expressed by Sino Biological Inc. For flow cytometry: To examine the binding of TCRγ4δ1-Fc with various cells, including HepG2, BGC-803, K562, Raji, PBMCs, ccc-HEL-1, 1308.1.86 and 293T cells, the tested cells were individually incubated with TCRγ4δ1-Fc for 60 min at 4°C. .. FITC-conjugated goat anti-human IgG antibody (ZSGB-bio, China) was added and incubated for 30 min at 4°C; human IgG-Fc was used as a control.

    Binding Assay:

    Article Title: TCRγ4δ1-Engineered αβT Cells Exhibit Effective Antitumor Activity
    Article Snippet: Immunofluorescence was measured with an Accuri 6 Flow cytometer and analyzed by BD Accuri C6 software. .. TCRγ4δ1-Fc was constructed by fusing the complete extracellular domain of TCRγ4δ1 to the constant region of human IgG1 ( ) and expressed by Sino Biological Inc. For flow cytometry: To examine the binding of TCRγ4δ1-Fc with various cells, including HepG2, BGC-803, K562, Raji, PBMCs, ccc-HEL-1, 1308.1.86 and 293T cells, the tested cells were individually incubated with TCRγ4δ1-Fc for 60 min at 4°C. .. FITC-conjugated goat anti-human IgG antibody (ZSGB-bio, China) was added and incubated for 30 min at 4°C; human IgG-Fc was used as a control.

    Article Title: Blocking interaction between SHP2 and PD‐1 denotes a novel opportunity for developing PD‐1 inhibitors
    Article Snippet: After blocking the wells with 5% BSA in PBS, recombinant hSHP2‐FC (500 ng/ml, R & D Systems, 1894‐SH‐100) protein was added in the presence of phosphatase inhibitor cocktail and plates were incubated for 1 h at RT. .. Horseradish peroxidase (HRP)‐conjugated anti‐FC Tag antibody (1:5,000, Sino Biological, SSA001) was added in each well for 1 h, and binding was quantified using a DuoSet® Ancillary Reagent Kit (R & D Systems, DY008). ..

    Article Title: Integrating SpyCatcher/SpyTag covalent fusion technology into phage display workflows for rapid antibody discovery
    Article Snippet: All competitors were titrated in a 3-fold dilution series starting at 2.5 μg/ml and mixed 1:1 v/v (200 μl final) with in-house produced mSIRPα-Fc HEK supernatant before addition to the plate. .. After 1 h of incubation, binding of mSIRPα-Fc to plate-bound mCD47 was revealed by goat anti-human IgG HRP-conjugated antibody (Sino Biologicals, #SSA001; 1:5000 dilution) followed by the addition of TMB substrate for colorimetric detection. .. Surface plasmon resonance (SPR)SPR analysis was performed on a Biacore T200 instrument (GE Healthcare).

    Countercurrent Chromatography:

    Article Title: TCRγ4δ1-Engineered αβT Cells Exhibit Effective Antitumor Activity
    Article Snippet: Immunofluorescence was measured with an Accuri 6 Flow cytometer and analyzed by BD Accuri C6 software. .. TCRγ4δ1-Fc was constructed by fusing the complete extracellular domain of TCRγ4δ1 to the constant region of human IgG1 ( ) and expressed by Sino Biological Inc. For flow cytometry: To examine the binding of TCRγ4δ1-Fc with various cells, including HepG2, BGC-803, K562, Raji, PBMCs, ccc-HEL-1, 1308.1.86 and 293T cells, the tested cells were individually incubated with TCRγ4δ1-Fc for 60 min at 4°C. .. FITC-conjugated goat anti-human IgG antibody (ZSGB-bio, China) was added and incubated for 30 min at 4°C; human IgG-Fc was used as a control.

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  • 93
    Sino Biological goat anti human igg fc secondary antibody
    Study design and clinical measures of HEV vaccine response. a Immunization regimen and critical time points in the longitudinal analysis of human B-cell response to the HEV vaccine, Hecolin ® . The first, second, and third doses are indicated above the time axis at days 0, 31, and 183, respectively, with black arrows and needles. The seven time points selected for antibody-repertoire sequencing are indicated below the time axis with red arrows and “NGS.” Vaccine-induced, p239(1)-specific monoclonal antibodies (mAbs) were isolated from samples collected 1 month after the third dose (3M1), which is labeled below the time axis. Dynamics of anti-HEV <t>IgG</t> titer ( b ), anti-HEV IgG avidity ( c ), and serum neutralization measured by the 50% inhibitory dose (ID 50 ) ( d ) during vaccination. Error bars indicate standard deviation (s.d.) in ( b ). The dash lines represent the detection limit for the anti-HEV IgG and the threshold of neutralizing capacity for the serum neutralization assays. *Only anti-HEV IgG positive sera were tested for anti-HEV IgG avidity. Source data are provided in the Source Data file.
    Goat Anti Human Igg Fc Secondary Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti human igg fc secondary antibody/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti human igg fc secondary antibody - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

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    Study design and clinical measures of HEV vaccine response. a Immunization regimen and critical time points in the longitudinal analysis of human B-cell response to the HEV vaccine, Hecolin ® . The first, second, and third doses are indicated above the time axis at days 0, 31, and 183, respectively, with black arrows and needles. The seven time points selected for antibody-repertoire sequencing are indicated below the time axis with red arrows and “NGS.” Vaccine-induced, p239(1)-specific monoclonal antibodies (mAbs) were isolated from samples collected 1 month after the third dose (3M1), which is labeled below the time axis. Dynamics of anti-HEV IgG titer ( b ), anti-HEV IgG avidity ( c ), and serum neutralization measured by the 50% inhibitory dose (ID 50 ) ( d ) during vaccination. Error bars indicate standard deviation (s.d.) in ( b ). The dash lines represent the detection limit for the anti-HEV IgG and the threshold of neutralizing capacity for the serum neutralization assays. *Only anti-HEV IgG positive sera were tested for anti-HEV IgG avidity. Source data are provided in the Source Data file.

    Journal: Nature Communications

    Article Title: Quantitative evaluation of protective antibody response induced by hepatitis E vaccine in humans

    doi: 10.1038/s41467-020-17737-w

    Figure Lengend Snippet: Study design and clinical measures of HEV vaccine response. a Immunization regimen and critical time points in the longitudinal analysis of human B-cell response to the HEV vaccine, Hecolin ® . The first, second, and third doses are indicated above the time axis at days 0, 31, and 183, respectively, with black arrows and needles. The seven time points selected for antibody-repertoire sequencing are indicated below the time axis with red arrows and “NGS.” Vaccine-induced, p239(1)-specific monoclonal antibodies (mAbs) were isolated from samples collected 1 month after the third dose (3M1), which is labeled below the time axis. Dynamics of anti-HEV IgG titer ( b ), anti-HEV IgG avidity ( c ), and serum neutralization measured by the 50% inhibitory dose (ID 50 ) ( d ) during vaccination. Error bars indicate standard deviation (s.d.) in ( b ). The dash lines represent the detection limit for the anti-HEV IgG and the threshold of neutralizing capacity for the serum neutralization assays. *Only anti-HEV IgG positive sera were tested for anti-HEV IgG avidity. Source data are provided in the Source Data file.

    Article Snippet: The membranes were blocked with 5% skim milk and then incubated with sera or p239(1)-specific mAbs for 30 min. After rinse, the membranes were incubated with anti-human HRP-conjugated antibody (Sino Biological inc, Cat# SSA001).

    Techniques: Sequencing, Isolation, Labeling, Neutralization, Standard Deviation