influenza a h1n1  (Sino Biological)


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    Name:
    Influenza A H1N1 A Puerto Rico 8 1934 Hemagglutinin HA ELISA Pair Set
    Description:
    Each vial contains 130 ng of recombinant H1N1 A Puerto Rico 8 1934 HA Reconstitute with 1 mL detection antibody dilution buffer After reconstitution store at 20℃ to 80℃ in a manual defrost freezer A seven point standard curve using 2 fold serial dilutions in sample dilution buffer and a high standard of 3 ng mL is recommended
    Catalog Number:
    SEK11684
    Price:
    None
    Category:
    Elisa pair set
    Reactivity:
    Influenza
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    Structured Review

    Sino Biological influenza a h1n1
    Each vial contains 130 ng of recombinant H1N1 A Puerto Rico 8 1934 HA Reconstitute with 1 mL detection antibody dilution buffer After reconstitution store at 20℃ to 80℃ in a manual defrost freezer A seven point standard curve using 2 fold serial dilutions in sample dilution buffer and a high standard of 3 ng mL is recommended
    https://www.bioz.com/result/influenza a h1n1/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenza a h1n1 - by Bioz Stars, 2021-04
    94/100 stars

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    Recombinant:

    Article Title: Interferon-λ Mediates Non-redundant Front-Line Antiviral Protection against Influenza Virus Infection without Compromising Host Fitness.
    Article Snippet: STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d CONTACT FOR REAGENTS AND RESOURCES SHARING d EXPERIMENTAL MODEL AND SUBJECT DETAIL B Mice B Viruses d METHOD DETAILS B Infection of mice and treatments B Viral load assessment B Measurements of lung mechanisms B BAL analysis and differential cell counts B RNA isolation from lungs and qPCR B Validation of Ifnl2Egfp mice B Immunohistology and immunofluorescence B Flow cytometry and cell sorting B Neutrophil isolation and RNA-Seq analysis B Transcriptomics analyses d QUANTITATION AND STATISTICAL ANALYSIS d DATA AND SOFTWARE AVAILABILITY B Data Resources SUPPLEMENTAL INFORMATION Supplemental Information includes seven figures and four tables and can be found with this article at http://dx.doi.org/10.1016/j.immuni.2017.04.025. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER Mouse recombinant PEG-IFN lambda 2 Bristol-Myers Squibb N/A Poly(I:C) HMW Invivogen Cat#tlrl-pic A-Class CpG ODN1585 Invivogen Cat#tlrl-1585 Critical Commercial Assays TriReagent Sigma-Aldrich Cat#T9424 RQ1 DNase Promega Cat#M6101 M-MLV reverse transcriptase Promega Cat#M1701 RNeasy Micro kit QIAGEN Cat#74004 PrimeScript RT reagent Kit Takara Cat#RR037A iTaq Universal SYBR Green Supermix Biorad Cat#172-5124 TruSeq RNA Library Prep Kit v2 Illumina Cat#RS-122-2002 KAPA Library Quantification kit for Illumina Kapa Biosystems Cat#KK4903 Agilent DNA 1000 Kit Agilent Cat#5067-1504 Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA ELISA Pair Set Sino Biological Cat#SEK11684 IFN alpha Mouse ELISA Kit eBioscience Cat#BMS6027; RRID:AB_2575643 Mouse IFN-b ELISA Kit with Pre-coated Plates Biolegend Cat#439407 Mouse IL-28A/B (IFN-lambda 2/3) DuoSet ELISA R Systems Cat#DY1789B Mouse IL-6 ELISA MAX Deluxe Biolegend Cat#431305 Mouse TNF-a ELISA MAX Deluxe Biolegend Cat#430905 Milliplex Mouse Cytokine/Chemokine MAGNETIC Panel I Merck Millipore Cat#MCYTOMAG-70K Avidin/Biotin Blocking Kit Vector laboratories Cat#SP-2001; RRID:AB_2336231 TSA Kit #24, with HRP-Streptavidin and Alexa Fluor568 Tyramide ThermoFisher Scientific Cat#T20934 EasySepTM Mouse Neutrophil Enrichment Kit StemCell Technologies Cat#19762A Deposited Data Illumina Sequencing raw data (FASTQ) This paper GEO:PRJNA311166 Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:000664 Mouse: B6.129S2-Ifnar1tm1Agt/Mmjax, backcrossed to C57BL/6J (Ifnar1 / ) The Jackson Laboratory Cat#32045-JAX Mouse: B6.Cg-Tg(S100A8-cre,-EGFP)1Ilw/J The Jackson Laboratory RRID:IMSR_JAX:021614 Mouse: B6.Tg(Sftpc-cre)1Blh Okubo and Hogan, 2004 MGI ID:3574949 Mouse: B6.129(Cg)-Gt(ROSA)26Sortm4(ACTBtdTomato,-EGFP)Luo/J (mT/mG) The Jackson Laboratory RRID:IMSR_JAX:007676 Mouse: B6.IL-28RA / (Ifnlr1 / ) Ank et al., 2008 N/A Mouse: B6.Ifnlr1 / Ifnar1 / This paper N/A Mouse: B6.Ifnlr1fl/fl Lin et al., 2016 N/A Mouse: B6.S100A8CreIfnlr1fl/fl This paper N/A Mouse: B6.SftpcCreIfnlr1fl/fl This paper N/A Mouse: B6. .. SftpcCremT/mG This paper N/A Mouse: B6.Ifnl2-T2A-egfp (Ifnl2Egfp) This paper N/A Oligonucleotides See Table S4 This paper N/A Software and Algorithms FastQC http://www.bioinformatics. babraham.ac.uk/projects/fastqc/ RRID: SCR_014583 cutadapt https://cutadapt.readthedocs. io/en/stable/ RRID: SCR_011841 (Continued on next page) e2 Immunity 46, 875–890.e1–e6, May 16, 2017.

    SYBR Green Assay:

    Article Title: Interferon-λ Mediates Non-redundant Front-Line Antiviral Protection against Influenza Virus Infection without Compromising Host Fitness.
    Article Snippet: STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d CONTACT FOR REAGENTS AND RESOURCES SHARING d EXPERIMENTAL MODEL AND SUBJECT DETAIL B Mice B Viruses d METHOD DETAILS B Infection of mice and treatments B Viral load assessment B Measurements of lung mechanisms B BAL analysis and differential cell counts B RNA isolation from lungs and qPCR B Validation of Ifnl2Egfp mice B Immunohistology and immunofluorescence B Flow cytometry and cell sorting B Neutrophil isolation and RNA-Seq analysis B Transcriptomics analyses d QUANTITATION AND STATISTICAL ANALYSIS d DATA AND SOFTWARE AVAILABILITY B Data Resources SUPPLEMENTAL INFORMATION Supplemental Information includes seven figures and four tables and can be found with this article at http://dx.doi.org/10.1016/j.immuni.2017.04.025. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER Mouse recombinant PEG-IFN lambda 2 Bristol-Myers Squibb N/A Poly(I:C) HMW Invivogen Cat#tlrl-pic A-Class CpG ODN1585 Invivogen Cat#tlrl-1585 Critical Commercial Assays TriReagent Sigma-Aldrich Cat#T9424 RQ1 DNase Promega Cat#M6101 M-MLV reverse transcriptase Promega Cat#M1701 RNeasy Micro kit QIAGEN Cat#74004 PrimeScript RT reagent Kit Takara Cat#RR037A iTaq Universal SYBR Green Supermix Biorad Cat#172-5124 TruSeq RNA Library Prep Kit v2 Illumina Cat#RS-122-2002 KAPA Library Quantification kit for Illumina Kapa Biosystems Cat#KK4903 Agilent DNA 1000 Kit Agilent Cat#5067-1504 Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA ELISA Pair Set Sino Biological Cat#SEK11684 IFN alpha Mouse ELISA Kit eBioscience Cat#BMS6027; RRID:AB_2575643 Mouse IFN-b ELISA Kit with Pre-coated Plates Biolegend Cat#439407 Mouse IL-28A/B (IFN-lambda 2/3) DuoSet ELISA R Systems Cat#DY1789B Mouse IL-6 ELISA MAX Deluxe Biolegend Cat#431305 Mouse TNF-a ELISA MAX Deluxe Biolegend Cat#430905 Milliplex Mouse Cytokine/Chemokine MAGNETIC Panel I Merck Millipore Cat#MCYTOMAG-70K Avidin/Biotin Blocking Kit Vector laboratories Cat#SP-2001; RRID:AB_2336231 TSA Kit #24, with HRP-Streptavidin and Alexa Fluor568 Tyramide ThermoFisher Scientific Cat#T20934 EasySepTM Mouse Neutrophil Enrichment Kit StemCell Technologies Cat#19762A Deposited Data Illumina Sequencing raw data (FASTQ) This paper GEO:PRJNA311166 Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:000664 Mouse: B6.129S2-Ifnar1tm1Agt/Mmjax, backcrossed to C57BL/6J (Ifnar1 / ) The Jackson Laboratory Cat#32045-JAX Mouse: B6.Cg-Tg(S100A8-cre,-EGFP)1Ilw/J The Jackson Laboratory RRID:IMSR_JAX:021614 Mouse: B6.Tg(Sftpc-cre)1Blh Okubo and Hogan, 2004 MGI ID:3574949 Mouse: B6.129(Cg)-Gt(ROSA)26Sortm4(ACTBtdTomato,-EGFP)Luo/J (mT/mG) The Jackson Laboratory RRID:IMSR_JAX:007676 Mouse: B6.IL-28RA / (Ifnlr1 / ) Ank et al., 2008 N/A Mouse: B6.Ifnlr1 / Ifnar1 / This paper N/A Mouse: B6.Ifnlr1fl/fl Lin et al., 2016 N/A Mouse: B6.S100A8CreIfnlr1fl/fl This paper N/A Mouse: B6.SftpcCreIfnlr1fl/fl This paper N/A Mouse: B6. .. SftpcCremT/mG This paper N/A Mouse: B6.Ifnl2-T2A-egfp (Ifnl2Egfp) This paper N/A Oligonucleotides See Table S4 This paper N/A Software and Algorithms FastQC http://www.bioinformatics. babraham.ac.uk/projects/fastqc/ RRID: SCR_014583 cutadapt https://cutadapt.readthedocs. io/en/stable/ RRID: SCR_011841 (Continued on next page) e2 Immunity 46, 875–890.e1–e6, May 16, 2017.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Interferon-λ Mediates Non-redundant Front-Line Antiviral Protection against Influenza Virus Infection without Compromising Host Fitness.
    Article Snippet: STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d CONTACT FOR REAGENTS AND RESOURCES SHARING d EXPERIMENTAL MODEL AND SUBJECT DETAIL B Mice B Viruses d METHOD DETAILS B Infection of mice and treatments B Viral load assessment B Measurements of lung mechanisms B BAL analysis and differential cell counts B RNA isolation from lungs and qPCR B Validation of Ifnl2Egfp mice B Immunohistology and immunofluorescence B Flow cytometry and cell sorting B Neutrophil isolation and RNA-Seq analysis B Transcriptomics analyses d QUANTITATION AND STATISTICAL ANALYSIS d DATA AND SOFTWARE AVAILABILITY B Data Resources SUPPLEMENTAL INFORMATION Supplemental Information includes seven figures and four tables and can be found with this article at http://dx.doi.org/10.1016/j.immuni.2017.04.025. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER Mouse recombinant PEG-IFN lambda 2 Bristol-Myers Squibb N/A Poly(I:C) HMW Invivogen Cat#tlrl-pic A-Class CpG ODN1585 Invivogen Cat#tlrl-1585 Critical Commercial Assays TriReagent Sigma-Aldrich Cat#T9424 RQ1 DNase Promega Cat#M6101 M-MLV reverse transcriptase Promega Cat#M1701 RNeasy Micro kit QIAGEN Cat#74004 PrimeScript RT reagent Kit Takara Cat#RR037A iTaq Universal SYBR Green Supermix Biorad Cat#172-5124 TruSeq RNA Library Prep Kit v2 Illumina Cat#RS-122-2002 KAPA Library Quantification kit for Illumina Kapa Biosystems Cat#KK4903 Agilent DNA 1000 Kit Agilent Cat#5067-1504 Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA ELISA Pair Set Sino Biological Cat#SEK11684 IFN alpha Mouse ELISA Kit eBioscience Cat#BMS6027; RRID:AB_2575643 Mouse IFN-b ELISA Kit with Pre-coated Plates Biolegend Cat#439407 Mouse IL-28A/B (IFN-lambda 2/3) DuoSet ELISA R Systems Cat#DY1789B Mouse IL-6 ELISA MAX Deluxe Biolegend Cat#431305 Mouse TNF-a ELISA MAX Deluxe Biolegend Cat#430905 Milliplex Mouse Cytokine/Chemokine MAGNETIC Panel I Merck Millipore Cat#MCYTOMAG-70K Avidin/Biotin Blocking Kit Vector laboratories Cat#SP-2001; RRID:AB_2336231 TSA Kit #24, with HRP-Streptavidin and Alexa Fluor568 Tyramide ThermoFisher Scientific Cat#T20934 EasySepTM Mouse Neutrophil Enrichment Kit StemCell Technologies Cat#19762A Deposited Data Illumina Sequencing raw data (FASTQ) This paper GEO:PRJNA311166 Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:000664 Mouse: B6.129S2-Ifnar1tm1Agt/Mmjax, backcrossed to C57BL/6J (Ifnar1 / ) The Jackson Laboratory Cat#32045-JAX Mouse: B6.Cg-Tg(S100A8-cre,-EGFP)1Ilw/J The Jackson Laboratory RRID:IMSR_JAX:021614 Mouse: B6.Tg(Sftpc-cre)1Blh Okubo and Hogan, 2004 MGI ID:3574949 Mouse: B6.129(Cg)-Gt(ROSA)26Sortm4(ACTBtdTomato,-EGFP)Luo/J (mT/mG) The Jackson Laboratory RRID:IMSR_JAX:007676 Mouse: B6.IL-28RA / (Ifnlr1 / ) Ank et al., 2008 N/A Mouse: B6.Ifnlr1 / Ifnar1 / This paper N/A Mouse: B6.Ifnlr1fl/fl Lin et al., 2016 N/A Mouse: B6.S100A8CreIfnlr1fl/fl This paper N/A Mouse: B6.SftpcCreIfnlr1fl/fl This paper N/A Mouse: B6. .. SftpcCremT/mG This paper N/A Mouse: B6.Ifnl2-T2A-egfp (Ifnl2Egfp) This paper N/A Oligonucleotides See Table S4 This paper N/A Software and Algorithms FastQC http://www.bioinformatics. babraham.ac.uk/projects/fastqc/ RRID: SCR_014583 cutadapt https://cutadapt.readthedocs. io/en/stable/ RRID: SCR_011841 (Continued on next page) e2 Immunity 46, 875–890.e1–e6, May 16, 2017.

    Avidin-Biotin Assay:

    Article Title: Interferon-λ Mediates Non-redundant Front-Line Antiviral Protection against Influenza Virus Infection without Compromising Host Fitness.
    Article Snippet: STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d CONTACT FOR REAGENTS AND RESOURCES SHARING d EXPERIMENTAL MODEL AND SUBJECT DETAIL B Mice B Viruses d METHOD DETAILS B Infection of mice and treatments B Viral load assessment B Measurements of lung mechanisms B BAL analysis and differential cell counts B RNA isolation from lungs and qPCR B Validation of Ifnl2Egfp mice B Immunohistology and immunofluorescence B Flow cytometry and cell sorting B Neutrophil isolation and RNA-Seq analysis B Transcriptomics analyses d QUANTITATION AND STATISTICAL ANALYSIS d DATA AND SOFTWARE AVAILABILITY B Data Resources SUPPLEMENTAL INFORMATION Supplemental Information includes seven figures and four tables and can be found with this article at http://dx.doi.org/10.1016/j.immuni.2017.04.025. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER Mouse recombinant PEG-IFN lambda 2 Bristol-Myers Squibb N/A Poly(I:C) HMW Invivogen Cat#tlrl-pic A-Class CpG ODN1585 Invivogen Cat#tlrl-1585 Critical Commercial Assays TriReagent Sigma-Aldrich Cat#T9424 RQ1 DNase Promega Cat#M6101 M-MLV reverse transcriptase Promega Cat#M1701 RNeasy Micro kit QIAGEN Cat#74004 PrimeScript RT reagent Kit Takara Cat#RR037A iTaq Universal SYBR Green Supermix Biorad Cat#172-5124 TruSeq RNA Library Prep Kit v2 Illumina Cat#RS-122-2002 KAPA Library Quantification kit for Illumina Kapa Biosystems Cat#KK4903 Agilent DNA 1000 Kit Agilent Cat#5067-1504 Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA ELISA Pair Set Sino Biological Cat#SEK11684 IFN alpha Mouse ELISA Kit eBioscience Cat#BMS6027; RRID:AB_2575643 Mouse IFN-b ELISA Kit with Pre-coated Plates Biolegend Cat#439407 Mouse IL-28A/B (IFN-lambda 2/3) DuoSet ELISA R Systems Cat#DY1789B Mouse IL-6 ELISA MAX Deluxe Biolegend Cat#431305 Mouse TNF-a ELISA MAX Deluxe Biolegend Cat#430905 Milliplex Mouse Cytokine/Chemokine MAGNETIC Panel I Merck Millipore Cat#MCYTOMAG-70K Avidin/Biotin Blocking Kit Vector laboratories Cat#SP-2001; RRID:AB_2336231 TSA Kit #24, with HRP-Streptavidin and Alexa Fluor568 Tyramide ThermoFisher Scientific Cat#T20934 EasySepTM Mouse Neutrophil Enrichment Kit StemCell Technologies Cat#19762A Deposited Data Illumina Sequencing raw data (FASTQ) This paper GEO:PRJNA311166 Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:000664 Mouse: B6.129S2-Ifnar1tm1Agt/Mmjax, backcrossed to C57BL/6J (Ifnar1 / ) The Jackson Laboratory Cat#32045-JAX Mouse: B6.Cg-Tg(S100A8-cre,-EGFP)1Ilw/J The Jackson Laboratory RRID:IMSR_JAX:021614 Mouse: B6.Tg(Sftpc-cre)1Blh Okubo and Hogan, 2004 MGI ID:3574949 Mouse: B6.129(Cg)-Gt(ROSA)26Sortm4(ACTBtdTomato,-EGFP)Luo/J (mT/mG) The Jackson Laboratory RRID:IMSR_JAX:007676 Mouse: B6.IL-28RA / (Ifnlr1 / ) Ank et al., 2008 N/A Mouse: B6.Ifnlr1 / Ifnar1 / This paper N/A Mouse: B6.Ifnlr1fl/fl Lin et al., 2016 N/A Mouse: B6.S100A8CreIfnlr1fl/fl This paper N/A Mouse: B6.SftpcCreIfnlr1fl/fl This paper N/A Mouse: B6. .. SftpcCremT/mG This paper N/A Mouse: B6.Ifnl2-T2A-egfp (Ifnl2Egfp) This paper N/A Oligonucleotides See Table S4 This paper N/A Software and Algorithms FastQC http://www.bioinformatics. babraham.ac.uk/projects/fastqc/ RRID: SCR_014583 cutadapt https://cutadapt.readthedocs. io/en/stable/ RRID: SCR_011841 (Continued on next page) e2 Immunity 46, 875–890.e1–e6, May 16, 2017.

    Blocking Assay:

    Article Title: Interferon-λ Mediates Non-redundant Front-Line Antiviral Protection against Influenza Virus Infection without Compromising Host Fitness.
    Article Snippet: STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d CONTACT FOR REAGENTS AND RESOURCES SHARING d EXPERIMENTAL MODEL AND SUBJECT DETAIL B Mice B Viruses d METHOD DETAILS B Infection of mice and treatments B Viral load assessment B Measurements of lung mechanisms B BAL analysis and differential cell counts B RNA isolation from lungs and qPCR B Validation of Ifnl2Egfp mice B Immunohistology and immunofluorescence B Flow cytometry and cell sorting B Neutrophil isolation and RNA-Seq analysis B Transcriptomics analyses d QUANTITATION AND STATISTICAL ANALYSIS d DATA AND SOFTWARE AVAILABILITY B Data Resources SUPPLEMENTAL INFORMATION Supplemental Information includes seven figures and four tables and can be found with this article at http://dx.doi.org/10.1016/j.immuni.2017.04.025. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER Mouse recombinant PEG-IFN lambda 2 Bristol-Myers Squibb N/A Poly(I:C) HMW Invivogen Cat#tlrl-pic A-Class CpG ODN1585 Invivogen Cat#tlrl-1585 Critical Commercial Assays TriReagent Sigma-Aldrich Cat#T9424 RQ1 DNase Promega Cat#M6101 M-MLV reverse transcriptase Promega Cat#M1701 RNeasy Micro kit QIAGEN Cat#74004 PrimeScript RT reagent Kit Takara Cat#RR037A iTaq Universal SYBR Green Supermix Biorad Cat#172-5124 TruSeq RNA Library Prep Kit v2 Illumina Cat#RS-122-2002 KAPA Library Quantification kit for Illumina Kapa Biosystems Cat#KK4903 Agilent DNA 1000 Kit Agilent Cat#5067-1504 Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA ELISA Pair Set Sino Biological Cat#SEK11684 IFN alpha Mouse ELISA Kit eBioscience Cat#BMS6027; RRID:AB_2575643 Mouse IFN-b ELISA Kit with Pre-coated Plates Biolegend Cat#439407 Mouse IL-28A/B (IFN-lambda 2/3) DuoSet ELISA R Systems Cat#DY1789B Mouse IL-6 ELISA MAX Deluxe Biolegend Cat#431305 Mouse TNF-a ELISA MAX Deluxe Biolegend Cat#430905 Milliplex Mouse Cytokine/Chemokine MAGNETIC Panel I Merck Millipore Cat#MCYTOMAG-70K Avidin/Biotin Blocking Kit Vector laboratories Cat#SP-2001; RRID:AB_2336231 TSA Kit #24, with HRP-Streptavidin and Alexa Fluor568 Tyramide ThermoFisher Scientific Cat#T20934 EasySepTM Mouse Neutrophil Enrichment Kit StemCell Technologies Cat#19762A Deposited Data Illumina Sequencing raw data (FASTQ) This paper GEO:PRJNA311166 Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:000664 Mouse: B6.129S2-Ifnar1tm1Agt/Mmjax, backcrossed to C57BL/6J (Ifnar1 / ) The Jackson Laboratory Cat#32045-JAX Mouse: B6.Cg-Tg(S100A8-cre,-EGFP)1Ilw/J The Jackson Laboratory RRID:IMSR_JAX:021614 Mouse: B6.Tg(Sftpc-cre)1Blh Okubo and Hogan, 2004 MGI ID:3574949 Mouse: B6.129(Cg)-Gt(ROSA)26Sortm4(ACTBtdTomato,-EGFP)Luo/J (mT/mG) The Jackson Laboratory RRID:IMSR_JAX:007676 Mouse: B6.IL-28RA / (Ifnlr1 / ) Ank et al., 2008 N/A Mouse: B6.Ifnlr1 / Ifnar1 / This paper N/A Mouse: B6.Ifnlr1fl/fl Lin et al., 2016 N/A Mouse: B6.S100A8CreIfnlr1fl/fl This paper N/A Mouse: B6.SftpcCreIfnlr1fl/fl This paper N/A Mouse: B6. .. SftpcCremT/mG This paper N/A Mouse: B6.Ifnl2-T2A-egfp (Ifnl2Egfp) This paper N/A Oligonucleotides See Table S4 This paper N/A Software and Algorithms FastQC http://www.bioinformatics. babraham.ac.uk/projects/fastqc/ RRID: SCR_014583 cutadapt https://cutadapt.readthedocs. io/en/stable/ RRID: SCR_011841 (Continued on next page) e2 Immunity 46, 875–890.e1–e6, May 16, 2017.

    Sequencing:

    Article Title: Interferon-λ Mediates Non-redundant Front-Line Antiviral Protection against Influenza Virus Infection without Compromising Host Fitness.
    Article Snippet: STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d CONTACT FOR REAGENTS AND RESOURCES SHARING d EXPERIMENTAL MODEL AND SUBJECT DETAIL B Mice B Viruses d METHOD DETAILS B Infection of mice and treatments B Viral load assessment B Measurements of lung mechanisms B BAL analysis and differential cell counts B RNA isolation from lungs and qPCR B Validation of Ifnl2Egfp mice B Immunohistology and immunofluorescence B Flow cytometry and cell sorting B Neutrophil isolation and RNA-Seq analysis B Transcriptomics analyses d QUANTITATION AND STATISTICAL ANALYSIS d DATA AND SOFTWARE AVAILABILITY B Data Resources SUPPLEMENTAL INFORMATION Supplemental Information includes seven figures and four tables and can be found with this article at http://dx.doi.org/10.1016/j.immuni.2017.04.025. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER Mouse recombinant PEG-IFN lambda 2 Bristol-Myers Squibb N/A Poly(I:C) HMW Invivogen Cat#tlrl-pic A-Class CpG ODN1585 Invivogen Cat#tlrl-1585 Critical Commercial Assays TriReagent Sigma-Aldrich Cat#T9424 RQ1 DNase Promega Cat#M6101 M-MLV reverse transcriptase Promega Cat#M1701 RNeasy Micro kit QIAGEN Cat#74004 PrimeScript RT reagent Kit Takara Cat#RR037A iTaq Universal SYBR Green Supermix Biorad Cat#172-5124 TruSeq RNA Library Prep Kit v2 Illumina Cat#RS-122-2002 KAPA Library Quantification kit for Illumina Kapa Biosystems Cat#KK4903 Agilent DNA 1000 Kit Agilent Cat#5067-1504 Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA ELISA Pair Set Sino Biological Cat#SEK11684 IFN alpha Mouse ELISA Kit eBioscience Cat#BMS6027; RRID:AB_2575643 Mouse IFN-b ELISA Kit with Pre-coated Plates Biolegend Cat#439407 Mouse IL-28A/B (IFN-lambda 2/3) DuoSet ELISA R Systems Cat#DY1789B Mouse IL-6 ELISA MAX Deluxe Biolegend Cat#431305 Mouse TNF-a ELISA MAX Deluxe Biolegend Cat#430905 Milliplex Mouse Cytokine/Chemokine MAGNETIC Panel I Merck Millipore Cat#MCYTOMAG-70K Avidin/Biotin Blocking Kit Vector laboratories Cat#SP-2001; RRID:AB_2336231 TSA Kit #24, with HRP-Streptavidin and Alexa Fluor568 Tyramide ThermoFisher Scientific Cat#T20934 EasySepTM Mouse Neutrophil Enrichment Kit StemCell Technologies Cat#19762A Deposited Data Illumina Sequencing raw data (FASTQ) This paper GEO:PRJNA311166 Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:000664 Mouse: B6.129S2-Ifnar1tm1Agt/Mmjax, backcrossed to C57BL/6J (Ifnar1 / ) The Jackson Laboratory Cat#32045-JAX Mouse: B6.Cg-Tg(S100A8-cre,-EGFP)1Ilw/J The Jackson Laboratory RRID:IMSR_JAX:021614 Mouse: B6.Tg(Sftpc-cre)1Blh Okubo and Hogan, 2004 MGI ID:3574949 Mouse: B6.129(Cg)-Gt(ROSA)26Sortm4(ACTBtdTomato,-EGFP)Luo/J (mT/mG) The Jackson Laboratory RRID:IMSR_JAX:007676 Mouse: B6.IL-28RA / (Ifnlr1 / ) Ank et al., 2008 N/A Mouse: B6.Ifnlr1 / Ifnar1 / This paper N/A Mouse: B6.Ifnlr1fl/fl Lin et al., 2016 N/A Mouse: B6.S100A8CreIfnlr1fl/fl This paper N/A Mouse: B6.SftpcCreIfnlr1fl/fl This paper N/A Mouse: B6. .. SftpcCremT/mG This paper N/A Mouse: B6.Ifnl2-T2A-egfp (Ifnl2Egfp) This paper N/A Oligonucleotides See Table S4 This paper N/A Software and Algorithms FastQC http://www.bioinformatics. babraham.ac.uk/projects/fastqc/ RRID: SCR_014583 cutadapt https://cutadapt.readthedocs. io/en/stable/ RRID: SCR_011841 (Continued on next page) e2 Immunity 46, 875–890.e1–e6, May 16, 2017.

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    Sino Biological influenza a h1n1
    Influenza A H1n1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenza a h1n1/product/Sino Biological
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