fgfr3  (Sino Biological)


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    • 92
    Name:
    FGFR3 Matched ELISA Antibody Pair Set Mouse
    Description:
    Each vial contains 334 ng of recombinant Mouse FGFR3 CD333 Reconstitute with 1 mL detection antibody dilution buffer After reconstitution store at 20℃ to 80℃ in a manual defrost freezer A seven point standard curve using 2 fold serial dilutions in sample dilution buffer and a high standard of 10000 pg mL is recommended
    Catalog Number:
    SEK50071
    Price:
    None
    Category:
    Elisa pair set
    Reactivity:
    Mouse
    Product Aliases:
    CD333 Matched ELISA Antibody Pair Set Mouse, Fgfr-3 Matched ELISA Antibody Pair Set Mouse, Flg-2 Matched ELISA Antibody Pair Set Mouse, FR3 Matched ELISA Antibody Pair Set Mouse, HBGFR Matched ELISA Antibody Pair Set Mouse, Mfr3 Matched ELISA Antibody Pair Set Mouse, sam3 Matched ELISA Antibody Pair Set Mouse
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    Structured Review

    Sino Biological fgfr3
    Binding of PTK7 to FGFR family members. HA-tagged FGFR1, FGFR2, <t>FGFR3,</t> and FGFR4 (FGFR1-HA, FGFR2-HA, FGFR3-HA, and FGFR4-HA) were immunoprecipitated from HEK293 cells. FLAG-tagged PTK7 (PTK7-FLAG) binding to each FGFR was evaluated by Western blot. An empty vector served as the negative control. GAPDH served as a loading control for total cell lysate blots. The lower panel illustrates quantification of the binding of PTK7 to FGFRs. Each bar represents the mean ± sd from 3 independent experiments.
    Each vial contains 334 ng of recombinant Mouse FGFR3 CD333 Reconstitute with 1 mL detection antibody dilution buffer After reconstitution store at 20℃ to 80℃ in a manual defrost freezer A seven point standard curve using 2 fold serial dilutions in sample dilution buffer and a high standard of 10000 pg mL is recommended
    https://www.bioz.com/result/fgfr3/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fgfr3 - by Bioz Stars, 2021-04
    92/100 stars

    Images

    1) Product Images from "Catalytically inactive receptor tyrosine kinase PTK7 activates FGFR1 independent of FGF"

    Article Title: Catalytically inactive receptor tyrosine kinase PTK7 activates FGFR1 independent of FGF

    Journal: The FASEB Journal

    doi: 10.1096/fj.201900932R

    Binding of PTK7 to FGFR family members. HA-tagged FGFR1, FGFR2, FGFR3, and FGFR4 (FGFR1-HA, FGFR2-HA, FGFR3-HA, and FGFR4-HA) were immunoprecipitated from HEK293 cells. FLAG-tagged PTK7 (PTK7-FLAG) binding to each FGFR was evaluated by Western blot. An empty vector served as the negative control. GAPDH served as a loading control for total cell lysate blots. The lower panel illustrates quantification of the binding of PTK7 to FGFRs. Each bar represents the mean ± sd from 3 independent experiments.
    Figure Legend Snippet: Binding of PTK7 to FGFR family members. HA-tagged FGFR1, FGFR2, FGFR3, and FGFR4 (FGFR1-HA, FGFR2-HA, FGFR3-HA, and FGFR4-HA) were immunoprecipitated from HEK293 cells. FLAG-tagged PTK7 (PTK7-FLAG) binding to each FGFR was evaluated by Western blot. An empty vector served as the negative control. GAPDH served as a loading control for total cell lysate blots. The lower panel illustrates quantification of the binding of PTK7 to FGFRs. Each bar represents the mean ± sd from 3 independent experiments.

    Techniques Used: Binding Assay, Immunoprecipitation, Western Blot, Plasmid Preparation, Negative Control

    Related Articles

    Construct:

    Article Title: Catalytically inactive receptor tyrosine kinase PTK7 activates FGFR1 independent of FGF
    Article Snippet: The PCR product was ligated into the Hin dIII-XbaI sites of the pcDNA3.1 vector (Thermo Fisher Scientific). .. The pCMV3-FGFR2-HA, pCMV3-FGFR3-HA, and pCMV3-FGFR4-HA constructs encoding human FGFR2, FGFR3, and FGFR4 with a C-terminal HA-tag, respectively, were purchased from Sino Biological (Beijing, China). .. All generated constructs were sequenced to confirm they were error-free.

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    • fgfr3  (Sino Biological)
    92
    Sino Biological fgfr3
    Binding of PTK7 to FGFR family members. HA-tagged FGFR1, FGFR2, <t>FGFR3,</t> and FGFR4 (FGFR1-HA, FGFR2-HA, FGFR3-HA, and FGFR4-HA) were immunoprecipitated from HEK293 cells. FLAG-tagged PTK7 (PTK7-FLAG) binding to each FGFR was evaluated by Western blot. An empty vector served as the negative control. GAPDH served as a loading control for total cell lysate blots. The lower panel illustrates quantification of the binding of PTK7 to FGFRs. Each bar represents the mean ± sd from 3 independent experiments.
    Fgfr3, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fgfr3/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fgfr3 - by Bioz Stars, 2021-04
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Binding of PTK7 to FGFR family members. HA-tagged FGFR1, FGFR2, FGFR3, and FGFR4 (FGFR1-HA, FGFR2-HA, FGFR3-HA, and FGFR4-HA) were immunoprecipitated from HEK293 cells. FLAG-tagged PTK7 (PTK7-FLAG) binding to each FGFR was evaluated by Western blot. An empty vector served as the negative control. GAPDH served as a loading control for total cell lysate blots. The lower panel illustrates quantification of the binding of PTK7 to FGFRs. Each bar represents the mean ± sd from 3 independent experiments.

    Journal: The FASEB Journal

    Article Title: Catalytically inactive receptor tyrosine kinase PTK7 activates FGFR1 independent of FGF

    doi: 10.1096/fj.201900932R

    Figure Lengend Snippet: Binding of PTK7 to FGFR family members. HA-tagged FGFR1, FGFR2, FGFR3, and FGFR4 (FGFR1-HA, FGFR2-HA, FGFR3-HA, and FGFR4-HA) were immunoprecipitated from HEK293 cells. FLAG-tagged PTK7 (PTK7-FLAG) binding to each FGFR was evaluated by Western blot. An empty vector served as the negative control. GAPDH served as a loading control for total cell lysate blots. The lower panel illustrates quantification of the binding of PTK7 to FGFRs. Each bar represents the mean ± sd from 3 independent experiments.

    Article Snippet: The pCMV3-FGFR2-HA, pCMV3-FGFR3-HA, and pCMV3-FGFR4-HA constructs encoding human FGFR2, FGFR3, and FGFR4 with a C-terminal HA-tag, respectively, were purchased from Sino Biological (Beijing, China).

    Techniques: Binding Assay, Immunoprecipitation, Western Blot, Plasmid Preparation, Negative Control