dcr 2  (Sino Biological)


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    DcR2 TRAIL R4 Matched ELISA Antibody Pair Set Human
    Description:
    Each vial contains 345 ng of recombinant Human TRAIL R4 CD264 TNFRSF10D Reconstitute with 1 mL detection antibody dilution buffer After reconstitution store at 20℃ to 80℃ in a manual defrost freezer A seven point standard curve using 2 fold serial dilutions in sample dilution buffer and a high standard of 15000 pg mL is recommended
    Catalog Number:
    SEK10413
    Price:
    None
    Category:
    Elisa pair set
    Reactivity:
    Human
    Product Aliases:
    CD264 Matched ELISA Antibody Pair Set Human, DCR2 Matched ELISA Antibody Pair Set Human, TRAIL-R4 Matched ELISA Antibody Pair Set Human, TRAILR4 Matched ELISA Antibody Pair Set Human, TRUNDD Matched ELISA Antibody Pair Set Human
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    Structured Review

    Sino Biological dcr 2
    Cross‐blocking studies with  DCR ‐2 and  UP ‐D2. Cells were incubated at the saturation point of a primary antibody or an equal concentration of an isotype control and then stained with  UP ‐D2  PE  at 80 ng·mL −1 . The  MFI  ratio is the comparison between primary antibody and isotype control groups. (A) The change in  UP ‐D2  PE  binding to  CD 300f C CHO  transfectants with different primary antibody staining. (B) Difference in  UP ‐D2 binding to  CD 300f SI 4 ‐ and  CD 300f C ‐transfected  CHO  cells, which were incubated with a saturating amount of  DCR ‐2 or isotype control prior to  UP ‐D2  PE . (C) Difference in  UP ‐D2 binding to  AML  cell lines, which were incubated with a saturating amount of  DCR ‐2 or isotype control prior to  UP ‐D2  PE . Error bars represent  SEM . Panel B was analyzed using a t‐test. Panel C was analyzed using a one‐way  ANOVA  with multiple comparisons between groups. *** P
    Each vial contains 345 ng of recombinant Human TRAIL R4 CD264 TNFRSF10D Reconstitute with 1 mL detection antibody dilution buffer After reconstitution store at 20℃ to 80℃ in a manual defrost freezer A seven point standard curve using 2 fold serial dilutions in sample dilution buffer and a high standard of 15000 pg mL is recommended
    https://www.bioz.com/result/dcr 2/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dcr 2 - by Bioz Stars, 2021-04
    93/100 stars

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    1) Product Images from "CD300f epitopes are specific targets for acute myeloid leukemia with monocytic differentiation"

    Article Title: CD300f epitopes are specific targets for acute myeloid leukemia with monocytic differentiation

    Journal: Molecular Oncology

    doi: 10.1002/1878-0261.12549

    Cross‐blocking studies with  DCR ‐2 and  UP ‐D2. Cells were incubated at the saturation point of a primary antibody or an equal concentration of an isotype control and then stained with  UP ‐D2  PE  at 80 ng·mL −1 . The  MFI  ratio is the comparison between primary antibody and isotype control groups. (A) The change in  UP ‐D2  PE  binding to  CD 300f C CHO  transfectants with different primary antibody staining. (B) Difference in  UP ‐D2 binding to  CD 300f SI 4 ‐ and  CD 300f C ‐transfected  CHO  cells, which were incubated with a saturating amount of  DCR ‐2 or isotype control prior to  UP ‐D2  PE . (C) Difference in  UP ‐D2 binding to  AML  cell lines, which were incubated with a saturating amount of  DCR ‐2 or isotype control prior to  UP ‐D2  PE . Error bars represent  SEM . Panel B was analyzed using a t‐test. Panel C was analyzed using a one‐way  ANOVA  with multiple comparisons between groups. *** P
    Figure Legend Snippet: Cross‐blocking studies with DCR ‐2 and UP ‐D2. Cells were incubated at the saturation point of a primary antibody or an equal concentration of an isotype control and then stained with UP ‐D2 PE at 80 ng·mL −1 . The MFI ratio is the comparison between primary antibody and isotype control groups. (A) The change in UP ‐D2 PE binding to CD 300f C CHO transfectants with different primary antibody staining. (B) Difference in UP ‐D2 binding to CD 300f SI 4 ‐ and CD 300f C ‐transfected CHO cells, which were incubated with a saturating amount of DCR ‐2 or isotype control prior to UP ‐D2 PE . (C) Difference in UP ‐D2 binding to AML cell lines, which were incubated with a saturating amount of DCR ‐2 or isotype control prior to UP ‐D2 PE . Error bars represent SEM . Panel B was analyzed using a t‐test. Panel C was analyzed using a one‐way ANOVA with multiple comparisons between groups. *** P

    Techniques Used: Blocking Assay, Incubation, Concentration Assay, Staining, Binding Assay, Transfection

    Binding of  DCR ‐2 to  CD 300f +  cells enhances the binding of  UP ‐D2 to monocytes, monocytic  AML , but not  CD 34 + HSPC  or nonmonocytic  AML .  CB  or primary frozen  AML  cells were incubated with  PBS , the saturation point of  DCR ‐2 (10 μg·mL −1 ), or an equal concentration of an isotype control. Following primary incubation, samples were stained with  UP ‐D2  PE  at 80 ng·mL −1 . Data for monocytes, lymphocytes, and  CD 34 + HSPC  were obtained from  CB . (A) Difference in  UP ‐D2  PE  binding across cell types when saturated with  DCR ‐2 or isotype control, compared to  PBS . (B) Differences in  UP ‐D2  PE  binding across  CD 34 +  cells between  CB , monocytic  AML , and nonmonocytic  AML . Error bars represent  SEM . Statistical analysis was performed with one‐way  ANOVA  with multiple comparisons between groups. * P
    Figure Legend Snippet: Binding of DCR ‐2 to CD 300f + cells enhances the binding of UP ‐D2 to monocytes, monocytic AML , but not CD 34 + HSPC or nonmonocytic AML . CB or primary frozen AML cells were incubated with PBS , the saturation point of DCR ‐2 (10 μg·mL −1 ), or an equal concentration of an isotype control. Following primary incubation, samples were stained with UP ‐D2 PE at 80 ng·mL −1 . Data for monocytes, lymphocytes, and CD 34 + HSPC were obtained from CB . (A) Difference in UP ‐D2 PE binding across cell types when saturated with DCR ‐2 or isotype control, compared to PBS . (B) Differences in UP ‐D2 PE binding across CD 34 + cells between CB , monocytic AML , and nonmonocytic AML . Error bars represent SEM . Statistical analysis was performed with one‐way ANOVA with multiple comparisons between groups. * P

    Techniques Used: Binding Assay, Incubation, Concentration Assay, Staining

    Related Articles

    Generated:

    Article Title: CD300f epitopes are specific targets for acute myeloid leukemia with monocytic differentiation
    Article Snippet: 2.1 Antibodies CD300f mAbs used were UP‐D1 (mouse IgG1,κ, eFluor 660 conjugate, Jomar), UP‐D2 (mouse IgG1, κ, PE conjugate and purified, BioLegend, San Diego, CA, USA), and 234903 (rat IgG2b, R & D Systems, Minneapolis, MN, USA). .. An in‐house mAb, DCR‐2 (IgG1,κ), was generated from a mouse immunized with CD300f Chinese hamster ovary (CHO) transfectants and boosted with recombinant human CD300f‐Fc protein (Sino Biologicals, Beijing, China). .. The polyclonal antibodies used were rabbit antibody to the peptide representing residues 63–92 of the canonical CD300f sequence (CLM‐1, Abcam) and goat anti‐human LMIR3 (leukocyte myeloid inhibitory receptor; gLMIR3, R & D Systems).

    Recombinant:

    Article Title: CD300f epitopes are specific targets for acute myeloid leukemia with monocytic differentiation
    Article Snippet: 2.1 Antibodies CD300f mAbs used were UP‐D1 (mouse IgG1,κ, eFluor 660 conjugate, Jomar), UP‐D2 (mouse IgG1, κ, PE conjugate and purified, BioLegend, San Diego, CA, USA), and 234903 (rat IgG2b, R & D Systems, Minneapolis, MN, USA). .. An in‐house mAb, DCR‐2 (IgG1,κ), was generated from a mouse immunized with CD300f Chinese hamster ovary (CHO) transfectants and boosted with recombinant human CD300f‐Fc protein (Sino Biologicals, Beijing, China). .. The polyclonal antibodies used were rabbit antibody to the peptide representing residues 63–92 of the canonical CD300f sequence (CLM‐1, Abcam) and goat anti‐human LMIR3 (leukocyte myeloid inhibitory receptor; gLMIR3, R & D Systems).

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    Sino Biological dcr 2
    Cross‐blocking studies with  DCR ‐2 and  UP ‐D2. Cells were incubated at the saturation point of a primary antibody or an equal concentration of an isotype control and then stained with  UP ‐D2  PE  at 80 ng·mL −1 . The  MFI  ratio is the comparison between primary antibody and isotype control groups. (A) The change in  UP ‐D2  PE  binding to  CD 300f C CHO  transfectants with different primary antibody staining. (B) Difference in  UP ‐D2 binding to  CD 300f SI 4 ‐ and  CD 300f C ‐transfected  CHO  cells, which were incubated with a saturating amount of  DCR ‐2 or isotype control prior to  UP ‐D2  PE . (C) Difference in  UP ‐D2 binding to  AML  cell lines, which were incubated with a saturating amount of  DCR ‐2 or isotype control prior to  UP ‐D2  PE . Error bars represent  SEM . Panel B was analyzed using a t‐test. Panel C was analyzed using a one‐way  ANOVA  with multiple comparisons between groups. *** P
    Dcr 2, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dcr 2/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dcr 2 - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

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    Cross‐blocking studies with  DCR ‐2 and  UP ‐D2. Cells were incubated at the saturation point of a primary antibody or an equal concentration of an isotype control and then stained with  UP ‐D2  PE  at 80 ng·mL −1 . The  MFI  ratio is the comparison between primary antibody and isotype control groups. (A) The change in  UP ‐D2  PE  binding to  CD 300f C CHO  transfectants with different primary antibody staining. (B) Difference in  UP ‐D2 binding to  CD 300f SI 4 ‐ and  CD 300f C ‐transfected  CHO  cells, which were incubated with a saturating amount of  DCR ‐2 or isotype control prior to  UP ‐D2  PE . (C) Difference in  UP ‐D2 binding to  AML  cell lines, which were incubated with a saturating amount of  DCR ‐2 or isotype control prior to  UP ‐D2  PE . Error bars represent  SEM . Panel B was analyzed using a t‐test. Panel C was analyzed using a one‐way  ANOVA  with multiple comparisons between groups. *** P

    Journal: Molecular Oncology

    Article Title: CD300f epitopes are specific targets for acute myeloid leukemia with monocytic differentiation

    doi: 10.1002/1878-0261.12549

    Figure Lengend Snippet: Cross‐blocking studies with DCR ‐2 and UP ‐D2. Cells were incubated at the saturation point of a primary antibody or an equal concentration of an isotype control and then stained with UP ‐D2 PE at 80 ng·mL −1 . The MFI ratio is the comparison between primary antibody and isotype control groups. (A) The change in UP ‐D2 PE binding to CD 300f C CHO transfectants with different primary antibody staining. (B) Difference in UP ‐D2 binding to CD 300f SI 4 ‐ and CD 300f C ‐transfected CHO cells, which were incubated with a saturating amount of DCR ‐2 or isotype control prior to UP ‐D2 PE . (C) Difference in UP ‐D2 binding to AML cell lines, which were incubated with a saturating amount of DCR ‐2 or isotype control prior to UP ‐D2 PE . Error bars represent SEM . Panel B was analyzed using a t‐test. Panel C was analyzed using a one‐way ANOVA with multiple comparisons between groups. *** P

    Article Snippet: An in‐house mAb, DCR‐2 (IgG1,κ), was generated from a mouse immunized with CD300f Chinese hamster ovary (CHO) transfectants and boosted with recombinant human CD300f‐Fc protein (Sino Biologicals, Beijing, China).

    Techniques: Blocking Assay, Incubation, Concentration Assay, Staining, Binding Assay, Transfection

    Binding of  DCR ‐2 to  CD 300f +  cells enhances the binding of  UP ‐D2 to monocytes, monocytic  AML , but not  CD 34 + HSPC  or nonmonocytic  AML .  CB  or primary frozen  AML  cells were incubated with  PBS , the saturation point of  DCR ‐2 (10 μg·mL −1 ), or an equal concentration of an isotype control. Following primary incubation, samples were stained with  UP ‐D2  PE  at 80 ng·mL −1 . Data for monocytes, lymphocytes, and  CD 34 + HSPC  were obtained from  CB . (A) Difference in  UP ‐D2  PE  binding across cell types when saturated with  DCR ‐2 or isotype control, compared to  PBS . (B) Differences in  UP ‐D2  PE  binding across  CD 34 +  cells between  CB , monocytic  AML , and nonmonocytic  AML . Error bars represent  SEM . Statistical analysis was performed with one‐way  ANOVA  with multiple comparisons between groups. * P

    Journal: Molecular Oncology

    Article Title: CD300f epitopes are specific targets for acute myeloid leukemia with monocytic differentiation

    doi: 10.1002/1878-0261.12549

    Figure Lengend Snippet: Binding of DCR ‐2 to CD 300f + cells enhances the binding of UP ‐D2 to monocytes, monocytic AML , but not CD 34 + HSPC or nonmonocytic AML . CB or primary frozen AML cells were incubated with PBS , the saturation point of DCR ‐2 (10 μg·mL −1 ), or an equal concentration of an isotype control. Following primary incubation, samples were stained with UP ‐D2 PE at 80 ng·mL −1 . Data for monocytes, lymphocytes, and CD 34 + HSPC were obtained from CB . (A) Difference in UP ‐D2 PE binding across cell types when saturated with DCR ‐2 or isotype control, compared to PBS . (B) Differences in UP ‐D2 PE binding across CD 34 + cells between CB , monocytic AML , and nonmonocytic AML . Error bars represent SEM . Statistical analysis was performed with one‐way ANOVA with multiple comparisons between groups. * P

    Article Snippet: An in‐house mAb, DCR‐2 (IgG1,κ), was generated from a mouse immunized with CD300f Chinese hamster ovary (CHO) transfectants and boosted with recombinant human CD300f‐Fc protein (Sino Biologicals, Beijing, China).

    Techniques: Binding Assay, Incubation, Concentration Assay, Staining