clip cell tmr star  (New England Biolabs)


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    Name:
    CLIP Cell TMR Star
    Description:
    CLIP Cell TMR Star 30 nmol
    Catalog Number:
    s9219s
    Price:
    344
    Size:
    30 nmol
    Category:
    Fluorochromes
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    Structured Review

    New England Biolabs clip cell tmr star
    CLIP Cell TMR Star
    CLIP Cell TMR Star 30 nmol
    https://www.bioz.com/result/clip cell tmr star/product/New England Biolabs
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    clip cell tmr star - by Bioz Stars, 2020-05
    93/100 stars

    Images

    1) Product Images from "Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons"

    Article Title: Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0200759

    Detail comparison of SS02565 neuronal membrane labeling. All samples show the same region of projections crossing from the left optic lobe to the central brain. Only the neuronal membrane channel is shown, and is labeled via antibodies in (A-B) and CLIP-tag in (C-D). (A) SS02565 was crossed to w;; 5XUAS-IVS-myr :: smFLAG in VK00005 , pJFRC51-3XUAS-IVS-Syt :: smHA in su(Hw)attP1 and brains were labeled with pure IHC, including rat anti-FLAG and ATTO 647N goat anti-rat antibodies over a period of 13 days. (B) SS02565 was crossed to w; brp-SNAP; 5XUAS-IVS-myr :: smFLAG in VK00005 , pJFRC51-3XUAS-IVS-Syt :: smHA in su(Hw)attP1 and brains were labeled with hybrid IHC, including rat anti-FLAG and ATTO 647N goat anti-rat antibodies over a period of 6 days. (C) SS02565 was crossed to w; brp-SNAP; UAS-myr :: 4xCLIPf in VK00005 , UAS-Syt :: Halo7 in VK0027 and brains were labeled for 15 minutes with pure chemical tags, including TMR CLIP-tag ligand. (D) SS02565 was crossed to w; brp-SNAP; UAS-myr :: 4xCLIPf in VK00005 , UAS-Syt :: Halo7 in VK0027 and brains were labeled for 15 minutes with pure chemical tags, including JF 549 CLIP-tag ligand.
    Figure Legend Snippet: Detail comparison of SS02565 neuronal membrane labeling. All samples show the same region of projections crossing from the left optic lobe to the central brain. Only the neuronal membrane channel is shown, and is labeled via antibodies in (A-B) and CLIP-tag in (C-D). (A) SS02565 was crossed to w;; 5XUAS-IVS-myr :: smFLAG in VK00005 , pJFRC51-3XUAS-IVS-Syt :: smHA in su(Hw)attP1 and brains were labeled with pure IHC, including rat anti-FLAG and ATTO 647N goat anti-rat antibodies over a period of 13 days. (B) SS02565 was crossed to w; brp-SNAP; 5XUAS-IVS-myr :: smFLAG in VK00005 , pJFRC51-3XUAS-IVS-Syt :: smHA in su(Hw)attP1 and brains were labeled with hybrid IHC, including rat anti-FLAG and ATTO 647N goat anti-rat antibodies over a period of 6 days. (C) SS02565 was crossed to w; brp-SNAP; UAS-myr :: 4xCLIPf in VK00005 , UAS-Syt :: Halo7 in VK0027 and brains were labeled for 15 minutes with pure chemical tags, including TMR CLIP-tag ligand. (D) SS02565 was crossed to w; brp-SNAP; UAS-myr :: 4xCLIPf in VK00005 , UAS-Syt :: Halo7 in VK0027 and brains were labeled for 15 minutes with pure chemical tags, including JF 549 CLIP-tag ligand.

    Techniques Used: Labeling, Cross-linking Immunoprecipitation, Immunohistochemistry

    Comparison of Polarity IHC and chemical tag labeling methods. All samples show the Drosophila left optic lobe imaged at 63X. Each image is independently scaled for optimal intensity. (A). Polarity pure IHC : Split GAL4 SS02565 was crossed to w;; 5XUAS-IVS-myr :: smFLAG in VK00005 , pJFRC51-3XUAS-IVS-Syt :: smHA in su(Hw)attP1 and was labeled over a period of 13 days with nc82 mouse anti-Brp/Cy2 anti-mouse, rabbit anti-HA/Cy3 anti-rabbit, and rat anti-FLAG/Cy5 anti-rat. (B). Polarity hybrid IHC : SS02565 was crossed to w; brp-SNAP; 5XUAS-IVS-myr :: smFLAG in VK00005 , pJFRC51-3XUAS-IVS-Syt :: smHA in su(Hw)attP1 and labeled with Cy2 SNAP-tag ligand for 15 minutes, followed by rabbit anti-HA/Cy3 anti-rabbit, and rat anti-FLAG/Cy5 anti-rat over 6 days. Arrowheads indicate bleed-through of Cy5 into Cy2 channel. (C). Polarity pure chemical tag : SS02565 was crossed to w; brp-SNAP; UAS-myr :: 4xCLIPf in VK00005 , UAS-Syt :: Halo7 in VK0027 and labeled for 15 minutes with Cy2 SNAP-tag ligand, TMR CLIP-tag ligand, and ATTO 647N HaloTag ligand. (D). Polarity ATTO 647N pure IHC : As in (A) but with ATTO 647N instead of Cy5. (E). Polarity ATTO 647N hybrid IHC : As in (B) but with ATTO 647N instead of Cy5.
    Figure Legend Snippet: Comparison of Polarity IHC and chemical tag labeling methods. All samples show the Drosophila left optic lobe imaged at 63X. Each image is independently scaled for optimal intensity. (A). Polarity pure IHC : Split GAL4 SS02565 was crossed to w;; 5XUAS-IVS-myr :: smFLAG in VK00005 , pJFRC51-3XUAS-IVS-Syt :: smHA in su(Hw)attP1 and was labeled over a period of 13 days with nc82 mouse anti-Brp/Cy2 anti-mouse, rabbit anti-HA/Cy3 anti-rabbit, and rat anti-FLAG/Cy5 anti-rat. (B). Polarity hybrid IHC : SS02565 was crossed to w; brp-SNAP; 5XUAS-IVS-myr :: smFLAG in VK00005 , pJFRC51-3XUAS-IVS-Syt :: smHA in su(Hw)attP1 and labeled with Cy2 SNAP-tag ligand for 15 minutes, followed by rabbit anti-HA/Cy3 anti-rabbit, and rat anti-FLAG/Cy5 anti-rat over 6 days. Arrowheads indicate bleed-through of Cy5 into Cy2 channel. (C). Polarity pure chemical tag : SS02565 was crossed to w; brp-SNAP; UAS-myr :: 4xCLIPf in VK00005 , UAS-Syt :: Halo7 in VK0027 and labeled for 15 minutes with Cy2 SNAP-tag ligand, TMR CLIP-tag ligand, and ATTO 647N HaloTag ligand. (D). Polarity ATTO 647N pure IHC : As in (A) but with ATTO 647N instead of Cy5. (E). Polarity ATTO 647N hybrid IHC : As in (B) but with ATTO 647N instead of Cy5.

    Techniques Used: Immunohistochemistry, Labeling, Cross-linking Immunoprecipitation

    2) Product Images from "Snap-, CLIP- and Halo-Tag Labelling of Budding Yeast Cells"

    Article Title: Snap-, CLIP- and Halo-Tag Labelling of Budding Yeast Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0078745

    Labelling of SNAP-, CLIP- and Halo-tagged proteins in chemically fixed and living budding yeast cells. (A) Chemically fixed cells expressing the respective self-labelling proteins targeted to the mitochondrial matrix (mtSNAP, mtCLIP, or mtHalo) were labelled. (B) Labelling of live yeast cells expressing the mitochondrial targeted self-labelling proteins using an electroporation protocol. (C) Live yeast cells expressing the indicated fusion proteins labelled by electroporation. Cells were labelled using commercially available TMR substrates. Yeast strains expressing Abp1-SNAP and Pil1-CLIP were created by epitope-tagging, while the other fusion constructs were plasmid encoded. Shown are maximum projections of confocal sections. Scale bar: 2 µm.
    Figure Legend Snippet: Labelling of SNAP-, CLIP- and Halo-tagged proteins in chemically fixed and living budding yeast cells. (A) Chemically fixed cells expressing the respective self-labelling proteins targeted to the mitochondrial matrix (mtSNAP, mtCLIP, or mtHalo) were labelled. (B) Labelling of live yeast cells expressing the mitochondrial targeted self-labelling proteins using an electroporation protocol. (C) Live yeast cells expressing the indicated fusion proteins labelled by electroporation. Cells were labelled using commercially available TMR substrates. Yeast strains expressing Abp1-SNAP and Pil1-CLIP were created by epitope-tagging, while the other fusion constructs were plasmid encoded. Shown are maximum projections of confocal sections. Scale bar: 2 µm.

    Techniques Used: Cross-linking Immunoprecipitation, Expressing, Electroporation, Construct, Plasmid Preparation

    Live cell super-resolution microscopy and multi-colour microscopy using self-labelling proteins. (A) Living yeast cells expressing Pil1-CLIP at a near native level from the endogenous chromosomal locus were labelled by electroporation with Atto565-CLIP and imaged using confocal (left) and STED (right) microscopy. Inset: Intensity profile over the region marked with the arrow heads. (B) Dual colour labelling with the CLIP- and the Halo-tag. mtHalo was labelled with 6′-CR110-Halo and Pil1-CLIP was labelled with CLIP-Cell TMR-Star and imaged by epifluorescence microscopy. Scale bars: 2 µm.
    Figure Legend Snippet: Live cell super-resolution microscopy and multi-colour microscopy using self-labelling proteins. (A) Living yeast cells expressing Pil1-CLIP at a near native level from the endogenous chromosomal locus were labelled by electroporation with Atto565-CLIP and imaged using confocal (left) and STED (right) microscopy. Inset: Intensity profile over the region marked with the arrow heads. (B) Dual colour labelling with the CLIP- and the Halo-tag. mtHalo was labelled with 6′-CR110-Halo and Pil1-CLIP was labelled with CLIP-Cell TMR-Star and imaged by epifluorescence microscopy. Scale bars: 2 µm.

    Techniques Used: Microscopy, Expressing, Cross-linking Immunoprecipitation, Electroporation, Epifluorescence Microscopy

    3) Product Images from "Light-induced cell damage in live-cell super-resolution microscopy"

    Article Title: Light-induced cell damage in live-cell super-resolution microscopy

    Journal: Scientific Reports

    doi: 10.1038/srep15348

    Dependence of cell survival on irradiation intensity at 514 nm for 240 s of differently modified U2OS cells. ( a ) Wildtype cells irradiated at 21 °C, ( b ) cells stably transfected with CLIP-H2B irradiated at 21 °C, ( c ) cells stably transfected with CLIP-H2B and labeled with TMR, irradiated at 21 °C and ( d ) cells stably transfected with CLIP-H2B irradiated at 37 °C. Red dots are masked data points and were not considered for fitting. Error bars are given as one standard deviation. For each data point 20–50 cells were irradiated ( Table 1 ).
    Figure Legend Snippet: Dependence of cell survival on irradiation intensity at 514 nm for 240 s of differently modified U2OS cells. ( a ) Wildtype cells irradiated at 21 °C, ( b ) cells stably transfected with CLIP-H2B irradiated at 21 °C, ( c ) cells stably transfected with CLIP-H2B and labeled with TMR, irradiated at 21 °C and ( d ) cells stably transfected with CLIP-H2B irradiated at 37 °C. Red dots are masked data points and were not considered for fitting. Error bars are given as one standard deviation. For each data point 20–50 cells were irradiated ( Table 1 ).

    Techniques Used: Irradiation, Modification, Stable Transfection, Transfection, Cross-linking Immunoprecipitation, Labeling, Standard Deviation

    4) Product Images from "Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons"

    Article Title: Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0200759

    Detail comparison of SS02565 neuronal membrane labeling. All samples show the same region of projections crossing from the left optic lobe to the central brain. Only the neuronal membrane channel is shown, and is labeled via antibodies in (A-B) and CLIP-tag in (C-D). (A) SS02565 was crossed to w;; 5XUAS-IVS-myr :: smFLAG in VK00005 , pJFRC51-3XUAS-IVS-Syt :: smHA in su(Hw)attP1 and brains were labeled with pure IHC, including rat anti-FLAG and ATTO 647N goat anti-rat antibodies over a period of 13 days. (B) SS02565 was crossed to w; brp-SNAP; 5XUAS-IVS-myr :: smFLAG in VK00005 , pJFRC51-3XUAS-IVS-Syt :: smHA in su(Hw)attP1 and brains were labeled with hybrid IHC, including rat anti-FLAG and ATTO 647N goat anti-rat antibodies over a period of 6 days. (C) SS02565 was crossed to w; brp-SNAP; UAS-myr :: 4xCLIPf in VK00005 , UAS-Syt :: Halo7 in VK0027 and brains were labeled for 15 minutes with pure chemical tags, including TMR CLIP-tag ligand. (D) SS02565 was crossed to w; brp-SNAP; UAS-myr :: 4xCLIPf in VK00005 , UAS-Syt :: Halo7 in VK0027 and brains were labeled for 15 minutes with pure chemical tags, including JF 549 CLIP-tag ligand.
    Figure Legend Snippet: Detail comparison of SS02565 neuronal membrane labeling. All samples show the same region of projections crossing from the left optic lobe to the central brain. Only the neuronal membrane channel is shown, and is labeled via antibodies in (A-B) and CLIP-tag in (C-D). (A) SS02565 was crossed to w;; 5XUAS-IVS-myr :: smFLAG in VK00005 , pJFRC51-3XUAS-IVS-Syt :: smHA in su(Hw)attP1 and brains were labeled with pure IHC, including rat anti-FLAG and ATTO 647N goat anti-rat antibodies over a period of 13 days. (B) SS02565 was crossed to w; brp-SNAP; 5XUAS-IVS-myr :: smFLAG in VK00005 , pJFRC51-3XUAS-IVS-Syt :: smHA in su(Hw)attP1 and brains were labeled with hybrid IHC, including rat anti-FLAG and ATTO 647N goat anti-rat antibodies over a period of 6 days. (C) SS02565 was crossed to w; brp-SNAP; UAS-myr :: 4xCLIPf in VK00005 , UAS-Syt :: Halo7 in VK0027 and brains were labeled for 15 minutes with pure chemical tags, including TMR CLIP-tag ligand. (D) SS02565 was crossed to w; brp-SNAP; UAS-myr :: 4xCLIPf in VK00005 , UAS-Syt :: Halo7 in VK0027 and brains were labeled for 15 minutes with pure chemical tags, including JF 549 CLIP-tag ligand.

    Techniques Used: Labeling, Cross-linking Immunoprecipitation, Immunohistochemistry

    Comparison of Polarity IHC and chemical tag labeling methods. All samples show the Drosophila left optic lobe imaged at 63X. Each image is independently scaled for optimal intensity. (A). Polarity pure IHC : Split GAL4 SS02565 was crossed to w;; 5XUAS-IVS-myr :: smFLAG in VK00005 , pJFRC51-3XUAS-IVS-Syt :: smHA in su(Hw)attP1 and was labeled over a period of 13 days with nc82 mouse anti-Brp/Cy2 anti-mouse, rabbit anti-HA/Cy3 anti-rabbit, and rat anti-FLAG/Cy5 anti-rat. (B). Polarity hybrid IHC : SS02565 was crossed to w; brp-SNAP; 5XUAS-IVS-myr :: smFLAG in VK00005 , pJFRC51-3XUAS-IVS-Syt :: smHA in su(Hw)attP1 and labeled with Cy2 SNAP-tag ligand for 15 minutes, followed by rabbit anti-HA/Cy3 anti-rabbit, and rat anti-FLAG/Cy5 anti-rat over 6 days. Arrowheads indicate bleed-through of Cy5 into Cy2 channel. (C). Polarity pure chemical tag : SS02565 was crossed to w; brp-SNAP; UAS-myr :: 4xCLIPf in VK00005 , UAS-Syt :: Halo7 in VK0027 and labeled for 15 minutes with Cy2 SNAP-tag ligand, TMR CLIP-tag ligand, and ATTO 647N HaloTag ligand. (D). Polarity ATTO 647N pure IHC : As in (A) but with ATTO 647N instead of Cy5. (E). Polarity ATTO 647N hybrid IHC : As in (B) but with ATTO 647N instead of Cy5.
    Figure Legend Snippet: Comparison of Polarity IHC and chemical tag labeling methods. All samples show the Drosophila left optic lobe imaged at 63X. Each image is independently scaled for optimal intensity. (A). Polarity pure IHC : Split GAL4 SS02565 was crossed to w;; 5XUAS-IVS-myr :: smFLAG in VK00005 , pJFRC51-3XUAS-IVS-Syt :: smHA in su(Hw)attP1 and was labeled over a period of 13 days with nc82 mouse anti-Brp/Cy2 anti-mouse, rabbit anti-HA/Cy3 anti-rabbit, and rat anti-FLAG/Cy5 anti-rat. (B). Polarity hybrid IHC : SS02565 was crossed to w; brp-SNAP; 5XUAS-IVS-myr :: smFLAG in VK00005 , pJFRC51-3XUAS-IVS-Syt :: smHA in su(Hw)attP1 and labeled with Cy2 SNAP-tag ligand for 15 minutes, followed by rabbit anti-HA/Cy3 anti-rabbit, and rat anti-FLAG/Cy5 anti-rat over 6 days. Arrowheads indicate bleed-through of Cy5 into Cy2 channel. (C). Polarity pure chemical tag : SS02565 was crossed to w; brp-SNAP; UAS-myr :: 4xCLIPf in VK00005 , UAS-Syt :: Halo7 in VK0027 and labeled for 15 minutes with Cy2 SNAP-tag ligand, TMR CLIP-tag ligand, and ATTO 647N HaloTag ligand. (D). Polarity ATTO 647N pure IHC : As in (A) but with ATTO 647N instead of Cy5. (E). Polarity ATTO 647N hybrid IHC : As in (B) but with ATTO 647N instead of Cy5.

    Techniques Used: Immunohistochemistry, Labeling, Cross-linking Immunoprecipitation

    Related Articles

    Transfection:

    Article Title: Endosomal sorting by Semaphorin 4A in retinal pigment epithelium supports photoreceptor survival
    Article Snippet: .. SNAP-tagged and CLIP-tagged constructs expressed in transfected RPE cells were labeled for 30 min with 5 μM SNAP-Cell 505, 3 μM CLIP-Cell TMR-Star, and 3 μM CLIP-Cell 430 (New England BioLabs). ..

    Construct:

    Article Title: Endosomal sorting by Semaphorin 4A in retinal pigment epithelium supports photoreceptor survival
    Article Snippet: .. SNAP-tagged and CLIP-tagged constructs expressed in transfected RPE cells were labeled for 30 min with 5 μM SNAP-Cell 505, 3 μM CLIP-Cell TMR-Star, and 3 μM CLIP-Cell 430 (New England BioLabs). ..

    Purification:

    Article Title: Lipid and Protein Transfer between Nanolipoprotein Particles and Supported Lipid Bilayers
    Article Snippet: .. CLIP-ErbB2 NLPs were conjugated with fluorescent substrate CLIP-Cell TMR-Star (New England Biolabs), added to the cell-free reaction 1 hr prior to purification. .. All NLPs were isolated by nickel affinity purification.

    Labeling:

    Article Title: Endosomal sorting by Semaphorin 4A in retinal pigment epithelium supports photoreceptor survival
    Article Snippet: .. SNAP-tagged and CLIP-tagged constructs expressed in transfected RPE cells were labeled for 30 min with 5 μM SNAP-Cell 505, 3 μM CLIP-Cell TMR-Star, and 3 μM CLIP-Cell 430 (New England BioLabs). ..

    Staining:

    Article Title: Snap-, CLIP- and Halo-Tag Labelling of Budding Yeast Cells
    Article Snippet: .. Fluorescent CLIP-, SNAP- and Halo-tag ligands For the evaluation of the staining procedure, the commercially available fluorescent ligands SNAP-Cell TMR-Star, CLIP-Cell TMR-Star (isomer mixtures; New England Biolabs, Ipswich, MA, USA) and HaloTag-TMR ligand (6′-carboxy-isomer; Promega, Madison, WI, USA), were used. .. Alternatively, CLIP-, SNAP- and Halo-tag ligands were assembled from commercially available building blocks.

    Article Title: Light-induced cell damage in live-cell super-resolution microscopy
    Article Snippet: .. Cells were stained with 0.2 μM CLIP-Cell™ TMR-Star (New England Biolabs, cat. S9219S) for 30 minutes at 37 °C. ..

    Cross-linking Immunoprecipitation:

    Article Title: Snap-, CLIP- and Halo-Tag Labelling of Budding Yeast Cells
    Article Snippet: .. Fluorescent CLIP-, SNAP- and Halo-tag ligands For the evaluation of the staining procedure, the commercially available fluorescent ligands SNAP-Cell TMR-Star, CLIP-Cell TMR-Star (isomer mixtures; New England Biolabs, Ipswich, MA, USA) and HaloTag-TMR ligand (6′-carboxy-isomer; Promega, Madison, WI, USA), were used. .. Alternatively, CLIP-, SNAP- and Halo-tag ligands were assembled from commercially available building blocks.

    Article Title: Lipid and Protein Transfer between Nanolipoprotein Particles and Supported Lipid Bilayers
    Article Snippet: .. CLIP-ErbB2 NLPs were conjugated with fluorescent substrate CLIP-Cell TMR-Star (New England Biolabs), added to the cell-free reaction 1 hr prior to purification. .. All NLPs were isolated by nickel affinity purification.

    Article Title: Light-induced cell damage in live-cell super-resolution microscopy
    Article Snippet: .. Cells were stained with 0.2 μM CLIP-Cell™ TMR-Star (New England Biolabs, cat. S9219S) for 30 minutes at 37 °C. ..

    Article Title: Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons
    Article Snippet: .. In addition to the novel chemical tag ligands described here, we used CLIP-Cell TMR-Star (S9219S, New England Biolabs, Ipswich, MA) and JF549 SNAP-tag ligand [ ]. .. Hybrid IHC & chemical tag The hybrid IHC/chemical tag protocol combined Cy2 SNAP-tag ligand labeling of the brp-SNAP reference with antibody labeling of specific neurons.

    Article Title: Endosomal sorting by Semaphorin 4A in retinal pigment epithelium supports photoreceptor survival
    Article Snippet: .. SNAP-tagged and CLIP-tagged constructs expressed in transfected RPE cells were labeled for 30 min with 5 μM SNAP-Cell 505, 3 μM CLIP-Cell TMR-Star, and 3 μM CLIP-Cell 430 (New England BioLabs). ..

    Article Title: Self-Labeling Enzyme Tags for Analyses of Translocation of Type III Secretion System Effector Proteins
    Article Snippet: .. The self-labeling of SPI2-T3SS effector proteins fused with HaloTag, SNAP-tag, and CLIP-tag was done with HaloTag ligand TMR (HTL-TMR; Promega), SNAP-tag ligand TMR (SNAP-Cell TMR-Star; New England BioLabs [NEB]), and CLIP-tag ligand TMR (CLIP-Cell TMR-Star; NEB) (excitation wavelength, 545 nm; emission wavelength, 575 nm). .. For live-cell fluorescence microscopy, HeLa LAMP1-GFP cells were infected for 16 h in 8-well plates.

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  • 93
    New England Biolabs clip cell tmr star
    <t>CLIP-Cas9</t> activity and internalization in keratinocytes. ( a ) Schematic of CLIP-Cas9::sgRNA electroporation strategy. ( b ) Indel spectrum determined by TIDE of primary keratinocytes electroporated with CLIP-Cas9::sgRNA targeting the Atat1 gene. The inset show T7 endonuclease 1 assay performed on genomic DNA from electroporated keratinocytes. t.e. = total efficiency. ( c ) Quantification (% cells) and representative images ( d ) of <t>TMR</t> positive cells upon 2 hours treatment with 2 μM of ligand cross-linked Cas9 (#1 no sgRNA; #2 with sgRNA; #3 with sgRNA+ protamine; #4 with sgRNA + ppTG21). Nuclei were stained with Hoechst. Scale bars, 20 μm. The horizontal lines mark the geometric mean and the error bars mark the standard error.
    Clip Cell Tmr Star, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/clip cell tmr star/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    clip cell tmr star - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    CLIP-Cas9 activity and internalization in keratinocytes. ( a ) Schematic of CLIP-Cas9::sgRNA electroporation strategy. ( b ) Indel spectrum determined by TIDE of primary keratinocytes electroporated with CLIP-Cas9::sgRNA targeting the Atat1 gene. The inset show T7 endonuclease 1 assay performed on genomic DNA from electroporated keratinocytes. t.e. = total efficiency. ( c ) Quantification (% cells) and representative images ( d ) of TMR positive cells upon 2 hours treatment with 2 μM of ligand cross-linked Cas9 (#1 no sgRNA; #2 with sgRNA; #3 with sgRNA+ protamine; #4 with sgRNA + ppTG21). Nuclei were stained with Hoechst. Scale bars, 20 μm. The horizontal lines mark the geometric mean and the error bars mark the standard error.

    Journal: Scientific Reports

    Article Title: A ligand-based system for receptor-specific delivery of proteins

    doi: 10.1038/s41598-019-55797-1

    Figure Lengend Snippet: CLIP-Cas9 activity and internalization in keratinocytes. ( a ) Schematic of CLIP-Cas9::sgRNA electroporation strategy. ( b ) Indel spectrum determined by TIDE of primary keratinocytes electroporated with CLIP-Cas9::sgRNA targeting the Atat1 gene. The inset show T7 endonuclease 1 assay performed on genomic DNA from electroporated keratinocytes. t.e. = total efficiency. ( c ) Quantification (% cells) and representative images ( d ) of TMR positive cells upon 2 hours treatment with 2 μM of ligand cross-linked Cas9 (#1 no sgRNA; #2 with sgRNA; #3 with sgRNA+ protamine; #4 with sgRNA + ppTG21). Nuclei were stained with Hoechst. Scale bars, 20 μm. The horizontal lines mark the geometric mean and the error bars mark the standard error.

    Article Snippet: SDS-PAGE and western blotting To assess the coupling reaction, CLIP-Cre or CLIP-Cas9 were coupled with an excess of BC-Surface488 or BCTMR at a 1:1.5 molar ratio (NEB #S9232S; #S9219S) for 1 hour at 37 °C in PBS (pH 7.4).

    Techniques: Cross-linking Immunoprecipitation, Activity Assay, Electroporation, Staining

    Binding of SNAP-tagged ligands to keratinocytes and selective cross-linking to CLIP-tagged enzymes. ( a ) Schematic representation of one keratinocyte expressing the receptors of interest and the ligands used. ( b ) Quantification of labelled IL-31 K138A SNAP-BG 549 ( c ), NGF R121W SNAP-BG 549 ( d ) and SNAP-BG 549 (green bars) binding to primary keratinocytes. Nuclear localization was observed after 2 hours treatment. The nuclei were stained with Hoechst. Scale bars, 20 μm. The insets represent corresponding brightfield images. ( e ) 3D structures showing selective cross-linking of SNAP-tagged ligands (NGF-SNAP) and CLIP-tagged proteins (CLIP-Cre) through a BG-TMR-PEG-BC linker (PDB ID codes: 1BET, 1KBU, 3KZY). ( f ) Schematic representation of S-CROSS optimized chemical reaction. ( g ) Representative Coomassie gel showing cross-linking complexes (red asterisks). First lane (#1) is IL-31 SNAP::CLIP CRE, second lane (#2) is NGF SNAP::CLIP CRE and third lane (#3) is SNAP::CLIP-CRE. ( h ) Quantification of cross-linking from Coomassie gel ( g ).

    Journal: Scientific Reports

    Article Title: A ligand-based system for receptor-specific delivery of proteins

    doi: 10.1038/s41598-019-55797-1

    Figure Lengend Snippet: Binding of SNAP-tagged ligands to keratinocytes and selective cross-linking to CLIP-tagged enzymes. ( a ) Schematic representation of one keratinocyte expressing the receptors of interest and the ligands used. ( b ) Quantification of labelled IL-31 K138A SNAP-BG 549 ( c ), NGF R121W SNAP-BG 549 ( d ) and SNAP-BG 549 (green bars) binding to primary keratinocytes. Nuclear localization was observed after 2 hours treatment. The nuclei were stained with Hoechst. Scale bars, 20 μm. The insets represent corresponding brightfield images. ( e ) 3D structures showing selective cross-linking of SNAP-tagged ligands (NGF-SNAP) and CLIP-tagged proteins (CLIP-Cre) through a BG-TMR-PEG-BC linker (PDB ID codes: 1BET, 1KBU, 3KZY). ( f ) Schematic representation of S-CROSS optimized chemical reaction. ( g ) Representative Coomassie gel showing cross-linking complexes (red asterisks). First lane (#1) is IL-31 SNAP::CLIP CRE, second lane (#2) is NGF SNAP::CLIP CRE and third lane (#3) is SNAP::CLIP-CRE. ( h ) Quantification of cross-linking from Coomassie gel ( g ).

    Article Snippet: SDS-PAGE and western blotting To assess the coupling reaction, CLIP-Cre or CLIP-Cas9 were coupled with an excess of BC-Surface488 or BCTMR at a 1:1.5 molar ratio (NEB #S9232S; #S9219S) for 1 hour at 37 °C in PBS (pH 7.4).

    Techniques: Binding Assay, Cross-linking Immunoprecipitation, Expressing, Staining