snap surface 649 fluorophore (New England Biolabs)
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Name:
SNAP Surface 649
Description:
SNAP Surface 649 50 nmol
Catalog Number:
S9159S
Price:
344
Category:
Fluorochromes
Size:
50 nmol
|
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Structured Review
New England Biolabs
snap surface 649 fluorophore
SNAP Surface 649 50 nmol
https://www.bioz.com/result/snap surface 649 fluorophore/product/New England Biolabs
Average 98 stars, based on 1 article reviews
Price from $9.99 to $1999.99
SNAP Surface 649 50 nmol
https://www.bioz.com/result/snap surface 649 fluorophore/product/New England Biolabs
Average 98 stars, based on 1 article reviews
Price from $9.99 to $1999.99
snap surface 649 fluorophore - by Bioz Stars,
2021-06
98/100 stars
Images
1) Product Images from "Nuclear import receptor inhibits phase separation of FUS through binding to multiple sites"
Article Title: Nuclear import receptor inhibits phase separation of FUS through binding to multiple sites
Journal: Cell
doi: 10.1016/j.cell.2018.03.003
Figure Legend Snippet: Kapβ2 inhibits FUS turbidity and phase separation in a PY-NLS- and RanGTP-dependent manner A ) Domain organization of FUS. B ) Turbidity of 8 μM MBP-FUS ± 8 μM Kapβ2, measured for 60 min at room temperature after addition of Tev protease to remove MBP from MBP-FUS. C ) Turbidity of 8 μM MBP-FUS in the presence of buffer, 8 μM Kapβ2 ± RanGTP or inhibitor M9M, or Kapβ2Δloop ± RanGTP (60 min after Tev). D) Either 8 μM Kapβ2 or buffer was added at time=60 min to turbid FUS (8 μM MBP-FUS pre-treated with Tev for 60 min) and OD 395nm measured for the next 20 min. B )- D ), OD 395nm normalized to measurements of MBP-FU+buffer+Tev at time=60 min. C) - D) , mean of 3 technical replicates, ± S.D. E ) Mixtures containing 5 μM MBP-FUS, 0.5 μM MBP-FUS-SNAP SNAP-Surface 649 also shows FUS droplets at time=1 hr.) F ) Mixtures of 5 μM MBP-FUS and 0.5 μM MBP-FUS-SNAP SNAP-Surface 649 also shows the first 30 min after Kapβ2 addition). Images in E)-F) .
Techniques Used:
2) Product Images from "Acylation of the incretin peptide exendin-4 directly impacts GLP-1 receptor signalling and trafficking"
Article Title: Acylation of the incretin peptide exendin-4 directly impacts GLP-1 receptor signalling and trafficking
Journal: bioRxiv
doi: 10.1101/2021.04.01.438030
Figure Legend Snippet: Visualisation of GLP-1R endocytosis with exendin-4 and exendin-4-C16, and fluorescent agonist conjugates. ( A ) Representative images from n =3 experiments demonstrating endocytosis of SNAP-GLP-1R labelled with SNAP-Surface-549 after treatment for 30 min with 100 nM agonist or vehicle; scale bars = 8 µm. ( B ) Time-lapse images demonstrating movement of surface-labelled SNAP-GLP-1R into punctate structures on stimulation with 100 nM agonist or vehicle; scale bars = 20 µm. The graphs show the change over time in “spot count” per 0.33 mm field-of-view (FOV) after normalisation to cell confluence, with the concentration-dependent rate constant (K) derived from one-phase association fitting also plotted with a 3-parameter fit. ( C ) Representative images showing endosomal uptake of exendin-4 and exendin-4-TMR in T-REx-SNAP-GLP-1R cells with or without tetracycline-induced GLP-1R expression, labelled with SNAP-Surface-649 prior to stimulation with 100 nM of each TMR-conjugate for 30 min. Scale bars = 8 µm. Data are shown as mean ± SEM.
Techniques Used: Concentration Assay, Derivative Assay, Expressing
Related Articles
Fluorescence:Article Title: Tropomyosin isoforms differentially tune actin filament length and disassembly Article Snippet: After being washed with Buffer A (20 mM Tris, pH 7.5, 300 mM NaCl, 50 mM imidazole, and 1 mM DTT), proteins were eluted in Buffer A supplemented with 250 mM imidazole, concentrated, and purified further on a Superose 6 gel-filtration column (GE Healthcare Biosciences, Pittsburgh, PA) equilibrated in Buffer B (20 mM Tris, pH 8.0, 50 mM KCl, and 1 mM DTT). .. For fluorescence labeling of SNAP-Cor1B, the fusion protein was bound to Ni2+ -NTA beads, washed extensively in PBS with 1 mM DTT, and incubated with a fivefold excess of benzylguanine or Labeling:Article Title: Tropomyosin isoforms differentially tune actin filament length and disassembly Article Snippet: After being washed with Buffer A (20 mM Tris, pH 7.5, 300 mM NaCl, 50 mM imidazole, and 1 mM DTT), proteins were eluted in Buffer A supplemented with 250 mM imidazole, concentrated, and purified further on a Superose 6 gel-filtration column (GE Healthcare Biosciences, Pittsburgh, PA) equilibrated in Buffer B (20 mM Tris, pH 8.0, 50 mM KCl, and 1 mM DTT). .. For fluorescence labeling of SNAP-Cor1B, the fusion protein was bound to Ni2+ -NTA beads, washed extensively in PBS with 1 mM DTT, and incubated with a fivefold excess of benzylguanine or Article Title: Unusual mode of dimerization of retinitis pigmentosa-associated F220C rhodopsin Article Snippet: The purified protein was kept at 4°C for up to a week or snap-frozen in aliquots and stored at −80°C. .. FRET measurements in vitro WT and F220C opsins with a C-terminal FLAG-SNAP tag ( ) were capture onto anti-FLAG resin and labeled with either SNAP-Surface 549 (donor (D)) or Article Title: Single-molecule visualization of fast polymerase turnover in the bacterial replisome Article Snippet: .. Fluorescent labeling of SNAP-α Two different fluorescent probes, Article Title: Nuclear import receptor inhibits phase separation of FUS through binding to multiple sites Article Snippet: The RNA in each fraction was tracked by monitoring fluorescence emission at 520 nm from the 6-FAM tag (excited at 495 nm) using Varioskan plate reader (Thermo Fisher Scientific, Inc.). .. For imaging experiments, purified MBP-FUS-SNAP was labeled with Incubation:Article Title: Tropomyosin isoforms differentially tune actin filament length and disassembly Article Snippet: After being washed with Buffer A (20 mM Tris, pH 7.5, 300 mM NaCl, 50 mM imidazole, and 1 mM DTT), proteins were eluted in Buffer A supplemented with 250 mM imidazole, concentrated, and purified further on a Superose 6 gel-filtration column (GE Healthcare Biosciences, Pittsburgh, PA) equilibrated in Buffer B (20 mM Tris, pH 8.0, 50 mM KCl, and 1 mM DTT). .. For fluorescence labeling of SNAP-Cor1B, the fusion protein was bound to Ni2+ -NTA beads, washed extensively in PBS with 1 mM DTT, and incubated with a fivefold excess of benzylguanine or Article Title: Circuits that encode and guide alcohol-associated preference Article Snippet: Within 20 min of dissection, tissue was incubated in 2% paraformaldehyde (PFA) in S2 at room temperature for 55 min. After fixation, samples were rinsed with phosphate buffered saline with 0.5% Triton X-100 (PBT) and washed 4 times for 15 min at room temperature. .. Following PBT washes, PBT was removed and samples were incubated in SNAP substrate diluted in In Vitro:Article Title: Unusual mode of dimerization of retinitis pigmentosa-associated F220C rhodopsin Article Snippet: The purified protein was kept at 4°C for up to a week or snap-frozen in aliquots and stored at −80°C. .. FRET measurements in vitro WT and F220C opsins with a C-terminal FLAG-SNAP tag ( ) were capture onto anti-FLAG resin and labeled with either SNAP-Surface 549 (donor (D)) or Imaging:Article Title: Nuclear import receptor inhibits phase separation of FUS through binding to multiple sites Article Snippet: The RNA in each fraction was tracked by monitoring fluorescence emission at 520 nm from the 6-FAM tag (excited at 495 nm) using Varioskan plate reader (Thermo Fisher Scientific, Inc.). .. For imaging experiments, purified MBP-FUS-SNAP was labeled with Purification:Article Title: Nuclear import receptor inhibits phase separation of FUS through binding to multiple sites Article Snippet: The RNA in each fraction was tracked by monitoring fluorescence emission at 520 nm from the 6-FAM tag (excited at 495 nm) using Varioskan plate reader (Thermo Fisher Scientific, Inc.). .. For imaging experiments, purified MBP-FUS-SNAP was labeled with |