snap surface 649 fluorophore  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Name:
    SNAP Surface 649
    Description:
    SNAP Surface 649 50 nmol
    Catalog Number:
    S9159S
    Price:
    344
    Category:
    Fluorochromes
    Size:
    50 nmol
    Buy from Supplier


    Structured Review

    New England Biolabs snap surface 649 fluorophore
    SNAP Surface 649
    SNAP Surface 649 50 nmol
    https://www.bioz.com/result/snap surface 649 fluorophore/product/New England Biolabs
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snap surface 649 fluorophore - by Bioz Stars, 2021-06
    98/100 stars

    Images

    1) Product Images from "Nuclear import receptor inhibits phase separation of FUS through binding to multiple sites"

    Article Title: Nuclear import receptor inhibits phase separation of FUS through binding to multiple sites

    Journal: Cell

    doi: 10.1016/j.cell.2018.03.003

    Kapβ2 inhibits FUS turbidity and phase separation in a PY-NLS- and RanGTP-dependent manner A ) Domain organization of FUS. B ) Turbidity of 8 μM MBP-FUS ± 8 μM Kapβ2, measured for 60 min at room temperature after addition of Tev protease to remove MBP from MBP-FUS. C ) Turbidity of 8 μM MBP-FUS in the presence of buffer, 8 μM Kapβ2 ± RanGTP or inhibitor M9M, or Kapβ2Δloop ± RanGTP (60 min after Tev). D) Either 8 μM Kapβ2 or buffer was added at time=60 min to turbid FUS (8 μM MBP-FUS pre-treated with Tev for 60 min) and OD 395nm measured for the next 20 min. B )- D ), OD 395nm normalized to measurements of MBP-FU+buffer+Tev at time=60 min. C) - D) , mean of 3 technical replicates, ± S.D. E ) Mixtures containing 5 μM MBP-FUS, 0.5 μM MBP-FUS-SNAP SNAP-Surface 649 also shows FUS droplets at time=1 hr.) F ) Mixtures of 5 μM MBP-FUS and 0.5 μM MBP-FUS-SNAP SNAP-Surface 649 also shows the first 30 min after Kapβ2 addition). Images in E)-F) .
    Figure Legend Snippet: Kapβ2 inhibits FUS turbidity and phase separation in a PY-NLS- and RanGTP-dependent manner A ) Domain organization of FUS. B ) Turbidity of 8 μM MBP-FUS ± 8 μM Kapβ2, measured for 60 min at room temperature after addition of Tev protease to remove MBP from MBP-FUS. C ) Turbidity of 8 μM MBP-FUS in the presence of buffer, 8 μM Kapβ2 ± RanGTP or inhibitor M9M, or Kapβ2Δloop ± RanGTP (60 min after Tev). D) Either 8 μM Kapβ2 or buffer was added at time=60 min to turbid FUS (8 μM MBP-FUS pre-treated with Tev for 60 min) and OD 395nm measured for the next 20 min. B )- D ), OD 395nm normalized to measurements of MBP-FU+buffer+Tev at time=60 min. C) - D) , mean of 3 technical replicates, ± S.D. E ) Mixtures containing 5 μM MBP-FUS, 0.5 μM MBP-FUS-SNAP SNAP-Surface 649 also shows FUS droplets at time=1 hr.) F ) Mixtures of 5 μM MBP-FUS and 0.5 μM MBP-FUS-SNAP SNAP-Surface 649 also shows the first 30 min after Kapβ2 addition). Images in E)-F) .

    Techniques Used:

    2) Product Images from "Acylation of the incretin peptide exendin-4 directly impacts GLP-1 receptor signalling and trafficking"

    Article Title: Acylation of the incretin peptide exendin-4 directly impacts GLP-1 receptor signalling and trafficking

    Journal: bioRxiv

    doi: 10.1101/2021.04.01.438030

    Visualisation of GLP-1R endocytosis with exendin-4 and exendin-4-C16, and fluorescent agonist conjugates. ( A ) Representative images from n =3 experiments demonstrating endocytosis of SNAP-GLP-1R labelled with SNAP-Surface-549 after treatment for 30 min with 100 nM agonist or vehicle; scale bars = 8 µm. ( B ) Time-lapse images demonstrating movement of surface-labelled SNAP-GLP-1R into punctate structures on stimulation with 100 nM agonist or vehicle; scale bars = 20 µm. The graphs show the change over time in “spot count” per 0.33 mm field-of-view (FOV) after normalisation to cell confluence, with the concentration-dependent rate constant (K) derived from one-phase association fitting also plotted with a 3-parameter fit. ( C ) Representative images showing endosomal uptake of exendin-4 and exendin-4-TMR in T-REx-SNAP-GLP-1R cells with or without tetracycline-induced GLP-1R expression, labelled with SNAP-Surface-649 prior to stimulation with 100 nM of each TMR-conjugate for 30 min. Scale bars = 8 µm. Data are shown as mean ± SEM.
    Figure Legend Snippet: Visualisation of GLP-1R endocytosis with exendin-4 and exendin-4-C16, and fluorescent agonist conjugates. ( A ) Representative images from n =3 experiments demonstrating endocytosis of SNAP-GLP-1R labelled with SNAP-Surface-549 after treatment for 30 min with 100 nM agonist or vehicle; scale bars = 8 µm. ( B ) Time-lapse images demonstrating movement of surface-labelled SNAP-GLP-1R into punctate structures on stimulation with 100 nM agonist or vehicle; scale bars = 20 µm. The graphs show the change over time in “spot count” per 0.33 mm field-of-view (FOV) after normalisation to cell confluence, with the concentration-dependent rate constant (K) derived from one-phase association fitting also plotted with a 3-parameter fit. ( C ) Representative images showing endosomal uptake of exendin-4 and exendin-4-TMR in T-REx-SNAP-GLP-1R cells with or without tetracycline-induced GLP-1R expression, labelled with SNAP-Surface-649 prior to stimulation with 100 nM of each TMR-conjugate for 30 min. Scale bars = 8 µm. Data are shown as mean ± SEM.

    Techniques Used: Concentration Assay, Derivative Assay, Expressing

    Related Articles

    Fluorescence:

    Article Title: Tropomyosin isoforms differentially tune actin filament length and disassembly
    Article Snippet: After being washed with Buffer A (20 mM Tris, pH 7.5, 300 mM NaCl, 50 mM imidazole, and 1 mM DTT), proteins were eluted in Buffer A supplemented with 250 mM imidazole, concentrated, and purified further on a Superose 6 gel-filtration column (GE Healthcare Biosciences, Pittsburgh, PA) equilibrated in Buffer B (20 mM Tris, pH 8.0, 50 mM KCl, and 1 mM DTT). .. For fluorescence labeling of SNAP-Cor1B, the fusion protein was bound to Ni2+ -NTA beads, washed extensively in PBS with 1 mM DTT, and incubated with a fivefold excess of benzylguanine or benzylchloropyrimidine SNAP-Surface 649 (New England Biolabs, Ipswich, MA) for 2 h at room temperature. ..

    Labeling:

    Article Title: Tropomyosin isoforms differentially tune actin filament length and disassembly
    Article Snippet: After being washed with Buffer A (20 mM Tris, pH 7.5, 300 mM NaCl, 50 mM imidazole, and 1 mM DTT), proteins were eluted in Buffer A supplemented with 250 mM imidazole, concentrated, and purified further on a Superose 6 gel-filtration column (GE Healthcare Biosciences, Pittsburgh, PA) equilibrated in Buffer B (20 mM Tris, pH 8.0, 50 mM KCl, and 1 mM DTT). .. For fluorescence labeling of SNAP-Cor1B, the fusion protein was bound to Ni2+ -NTA beads, washed extensively in PBS with 1 mM DTT, and incubated with a fivefold excess of benzylguanine or benzylchloropyrimidine SNAP-Surface 649 (New England Biolabs, Ipswich, MA) for 2 h at room temperature. ..

    Article Title: Unusual mode of dimerization of retinitis pigmentosa-associated F220C rhodopsin
    Article Snippet: The purified protein was kept at 4°C for up to a week or snap-frozen in aliquots and stored at −80°C. .. FRET measurements in vitro WT and F220C opsins with a C-terminal FLAG-SNAP tag ( ) were capture onto anti-FLAG resin and labeled with either SNAP-Surface 549 (donor (D)) or SNAP-Surface 649 (acceptor (A)). .. The resin was washed to remove unreacted dye, and the labeled proteins were eluted with FLAG peptide as described abovce.

    Article Title: Single-molecule visualization of fast polymerase turnover in the bacterial replisome
    Article Snippet: .. Fluorescent labeling of SNAP-α Two different fluorescent probes, SNAP-Surface 649 (red) and SNAP-Surface Alexa Fluor 488 (green; New England Biolabs), were used to label SNAP-α. .. All labeling reactions were carried out using a twofold molar excess of dye with 27 μM SNAP-α in 1 ml of 50 mM Tris-HCl pH 7.6, 2 mM dithiothreitol, 100 mM NaCl, 5% (v /v ) glycerol (buffer Fα) for 2 hr at 23°C, followed by 6°C overnight with gentle rotation.

    Article Title: Nuclear import receptor inhibits phase separation of FUS through binding to multiple sites
    Article Snippet: The RNA in each fraction was tracked by monitoring fluorescence emission at 520 nm from the 6-FAM tag (excited at 495 nm) using Varioskan plate reader (Thermo Fisher Scientific, Inc.). .. For imaging experiments, purified MBP-FUS-SNAP was labeled with SNAP-Surface 649 fluorophore (New England BioLabs) by incubating with 5-fold excess fluorophore for 2 hours at room temperature. ..

    Incubation:

    Article Title: Tropomyosin isoforms differentially tune actin filament length and disassembly
    Article Snippet: After being washed with Buffer A (20 mM Tris, pH 7.5, 300 mM NaCl, 50 mM imidazole, and 1 mM DTT), proteins were eluted in Buffer A supplemented with 250 mM imidazole, concentrated, and purified further on a Superose 6 gel-filtration column (GE Healthcare Biosciences, Pittsburgh, PA) equilibrated in Buffer B (20 mM Tris, pH 8.0, 50 mM KCl, and 1 mM DTT). .. For fluorescence labeling of SNAP-Cor1B, the fusion protein was bound to Ni2+ -NTA beads, washed extensively in PBS with 1 mM DTT, and incubated with a fivefold excess of benzylguanine or benzylchloropyrimidine SNAP-Surface 649 (New England Biolabs, Ipswich, MA) for 2 h at room temperature. ..

    Article Title: Circuits that encode and guide alcohol-associated preference
    Article Snippet: Within 20 min of dissection, tissue was incubated in 2% paraformaldehyde (PFA) in S2 at room temperature for 55 min. After fixation, samples were rinsed with phosphate buffered saline with 0.5% Triton X-100 (PBT) and washed 4 times for 15 min at room temperature. .. Following PBT washes, PBT was removed and samples were incubated in SNAP substrate diluted in PBT (SNAP-Surface649, NEB S9159S; 1:1000) for 1 hr at room temperature. .. Samples were then rinsed and washed 3 times for 10 min at room temperature and then blocked in 5% heat-inactivated goat serum in PBT for 90 min at room temperature and incubated with primary antibodies (Rabbit α-GFP Polyclonal (1:1000), Life Tech #A11122, Rat α-HA Monoclonal (1:100), Roche #11867423001) for two overnights at 4°C.

    In Vitro:

    Article Title: Unusual mode of dimerization of retinitis pigmentosa-associated F220C rhodopsin
    Article Snippet: The purified protein was kept at 4°C for up to a week or snap-frozen in aliquots and stored at −80°C. .. FRET measurements in vitro WT and F220C opsins with a C-terminal FLAG-SNAP tag ( ) were capture onto anti-FLAG resin and labeled with either SNAP-Surface 549 (donor (D)) or SNAP-Surface 649 (acceptor (A)). .. The resin was washed to remove unreacted dye, and the labeled proteins were eluted with FLAG peptide as described abovce.

    Imaging:

    Article Title: Nuclear import receptor inhibits phase separation of FUS through binding to multiple sites
    Article Snippet: The RNA in each fraction was tracked by monitoring fluorescence emission at 520 nm from the 6-FAM tag (excited at 495 nm) using Varioskan plate reader (Thermo Fisher Scientific, Inc.). .. For imaging experiments, purified MBP-FUS-SNAP was labeled with SNAP-Surface 649 fluorophore (New England BioLabs) by incubating with 5-fold excess fluorophore for 2 hours at room temperature. ..

    Purification:

    Article Title: Nuclear import receptor inhibits phase separation of FUS through binding to multiple sites
    Article Snippet: The RNA in each fraction was tracked by monitoring fluorescence emission at 520 nm from the 6-FAM tag (excited at 495 nm) using Varioskan plate reader (Thermo Fisher Scientific, Inc.). .. For imaging experiments, purified MBP-FUS-SNAP was labeled with SNAP-Surface 649 fluorophore (New England BioLabs) by incubating with 5-fold excess fluorophore for 2 hours at room temperature. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    New England Biolabs snap surface 649 fluorophore
    Kapβ2 inhibits FUS turbidity and phase separation in a PY-NLS- and RanGTP-dependent manner A ) Domain organization of FUS. B ) Turbidity of 8 μM MBP-FUS ± 8 μM Kapβ2, measured for 60 min at room temperature after addition of Tev protease to remove MBP from MBP-FUS. C ) Turbidity of 8 μM MBP-FUS in the presence of buffer, 8 μM Kapβ2 ± RanGTP or inhibitor M9M, or Kapβ2Δloop ± RanGTP (60 min after Tev). D) Either 8 μM Kapβ2 or buffer was added at time=60 min to turbid FUS (8 μM MBP-FUS pre-treated with Tev for 60 min) and OD 395nm measured for the next 20 min. B )- D ), OD 395nm normalized to measurements of MBP-FU+buffer+Tev at time=60 min. C) - D) , mean of 3 technical replicates, ± S.D. E ) Mixtures containing 5 μM MBP-FUS, 0.5 μM <t>MBP-FUS-SNAP</t> SNAP-Surface 649 also shows FUS droplets at time=1 hr.) F ) Mixtures of 5 μM MBP-FUS and 0.5 μM MBP-FUS-SNAP SNAP-Surface 649 also shows the first 30 min after Kapβ2 addition). Images in E)-F) .
    Snap Surface 649 Fluorophore, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snap surface 649 fluorophore/product/New England Biolabs
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snap surface 649 fluorophore - by Bioz Stars, 2021-06
    98/100 stars
      Buy from Supplier

    Image Search Results


    Kapβ2 inhibits FUS turbidity and phase separation in a PY-NLS- and RanGTP-dependent manner A ) Domain organization of FUS. B ) Turbidity of 8 μM MBP-FUS ± 8 μM Kapβ2, measured for 60 min at room temperature after addition of Tev protease to remove MBP from MBP-FUS. C ) Turbidity of 8 μM MBP-FUS in the presence of buffer, 8 μM Kapβ2 ± RanGTP or inhibitor M9M, or Kapβ2Δloop ± RanGTP (60 min after Tev). D) Either 8 μM Kapβ2 or buffer was added at time=60 min to turbid FUS (8 μM MBP-FUS pre-treated with Tev for 60 min) and OD 395nm measured for the next 20 min. B )- D ), OD 395nm normalized to measurements of MBP-FU+buffer+Tev at time=60 min. C) - D) , mean of 3 technical replicates, ± S.D. E ) Mixtures containing 5 μM MBP-FUS, 0.5 μM MBP-FUS-SNAP SNAP-Surface 649 also shows FUS droplets at time=1 hr.) F ) Mixtures of 5 μM MBP-FUS and 0.5 μM MBP-FUS-SNAP SNAP-Surface 649 also shows the first 30 min after Kapβ2 addition). Images in E)-F) .

    Journal: Cell

    Article Title: Nuclear import receptor inhibits phase separation of FUS through binding to multiple sites

    doi: 10.1016/j.cell.2018.03.003

    Figure Lengend Snippet: Kapβ2 inhibits FUS turbidity and phase separation in a PY-NLS- and RanGTP-dependent manner A ) Domain organization of FUS. B ) Turbidity of 8 μM MBP-FUS ± 8 μM Kapβ2, measured for 60 min at room temperature after addition of Tev protease to remove MBP from MBP-FUS. C ) Turbidity of 8 μM MBP-FUS in the presence of buffer, 8 μM Kapβ2 ± RanGTP or inhibitor M9M, or Kapβ2Δloop ± RanGTP (60 min after Tev). D) Either 8 μM Kapβ2 or buffer was added at time=60 min to turbid FUS (8 μM MBP-FUS pre-treated with Tev for 60 min) and OD 395nm measured for the next 20 min. B )- D ), OD 395nm normalized to measurements of MBP-FU+buffer+Tev at time=60 min. C) - D) , mean of 3 technical replicates, ± S.D. E ) Mixtures containing 5 μM MBP-FUS, 0.5 μM MBP-FUS-SNAP SNAP-Surface 649 also shows FUS droplets at time=1 hr.) F ) Mixtures of 5 μM MBP-FUS and 0.5 μM MBP-FUS-SNAP SNAP-Surface 649 also shows the first 30 min after Kapβ2 addition). Images in E)-F) .

    Article Snippet: For imaging experiments, purified MBP-FUS-SNAP was labeled with SNAP-Surface 649 fluorophore (New England BioLabs) by incubating with 5-fold excess fluorophore for 2 hours at room temperature.

    Techniques:

    Visualisation of GLP-1R endocytosis with exendin-4 and exendin-4-C16, and fluorescent agonist conjugates. ( A ) Representative images from n =3 experiments demonstrating endocytosis of SNAP-GLP-1R labelled with SNAP-Surface-549 after treatment for 30 min with 100 nM agonist or vehicle; scale bars = 8 µm. ( B ) Time-lapse images demonstrating movement of surface-labelled SNAP-GLP-1R into punctate structures on stimulation with 100 nM agonist or vehicle; scale bars = 20 µm. The graphs show the change over time in “spot count” per 0.33 mm field-of-view (FOV) after normalisation to cell confluence, with the concentration-dependent rate constant (K) derived from one-phase association fitting also plotted with a 3-parameter fit. ( C ) Representative images showing endosomal uptake of exendin-4 and exendin-4-TMR in T-REx-SNAP-GLP-1R cells with or without tetracycline-induced GLP-1R expression, labelled with SNAP-Surface-649 prior to stimulation with 100 nM of each TMR-conjugate for 30 min. Scale bars = 8 µm. Data are shown as mean ± SEM.

    Journal: bioRxiv

    Article Title: Acylation of the incretin peptide exendin-4 directly impacts GLP-1 receptor signalling and trafficking

    doi: 10.1101/2021.04.01.438030

    Figure Lengend Snippet: Visualisation of GLP-1R endocytosis with exendin-4 and exendin-4-C16, and fluorescent agonist conjugates. ( A ) Representative images from n =3 experiments demonstrating endocytosis of SNAP-GLP-1R labelled with SNAP-Surface-549 after treatment for 30 min with 100 nM agonist or vehicle; scale bars = 8 µm. ( B ) Time-lapse images demonstrating movement of surface-labelled SNAP-GLP-1R into punctate structures on stimulation with 100 nM agonist or vehicle; scale bars = 20 µm. The graphs show the change over time in “spot count” per 0.33 mm field-of-view (FOV) after normalisation to cell confluence, with the concentration-dependent rate constant (K) derived from one-phase association fitting also plotted with a 3-parameter fit. ( C ) Representative images showing endosomal uptake of exendin-4 and exendin-4-TMR in T-REx-SNAP-GLP-1R cells with or without tetracycline-induced GLP-1R expression, labelled with SNAP-Surface-649 prior to stimulation with 100 nM of each TMR-conjugate for 30 min. Scale bars = 8 µm. Data are shown as mean ± SEM.

    Article Snippet: Cells were labelled with SNAP-Surface-649 (0.5 µM, from New England Biolabs, Hitchin, UK) for 30 min at 37°C.

    Techniques: Concentration Assay, Derivative Assay, Expressing