Journal: ACS biomaterials science & engineering

Article Title: An Optogenetic Platform to Dynamically Control the Stiffness of Collagen Hydrogels

doi: 10.1021/acsbiomaterials.0c01488

Figure Lengend Snippet: A) Three-piece functionalization platform consisting of a SNAP-tag fusion protein (Opto-SNAP), benzylguanine maleimide, and a cysteine-containing polymer. B) Activation of Optoprotein-SNAP-tag fusion proteins, when attached to polymers through the aforementioned system, increases polymer-polymer interactions, thus stiffening the gel.

Article Snippet: Additional samples, both TEV-treated and -untreated, were incubated with 100 µM BG-Maleimide (NEB, S9153S) overnight at room temperature, and dialyzed into buffer C to remove excess BG-Maleimide.

Techniques: Activation Assay

Journal: ACS biomaterials science & engineering

Article Title: An Optogenetic Platform to Dynamically Control the Stiffness of Collagen Hydrogels

doi: 10.1021/acsbiomaterials.0c01488

Figure Lengend Snippet: A) Three-piece functionalization platform consisting of a SNAP-tag fusion protein (Opto-SNAP), benzylguanine maleimide, and a cysteine-containing polymer. B) Activation of Optoprotein-SNAP-tag fusion proteins, when attached to polymers through the aforementioned system, increases polymer-polymer interactions, thus stiffening the gel.

Article Snippet: Cleaved samples were used for further characterization via Mass Spectrometry, incubation with BG-Maleimide (NEB, S9153S), or loaded onto a HiTrap High Performance Ni-NTA column (GE Healthcare) for separation.

Techniques: Activation Assay