building block cbg nh2  (New England Biolabs)


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  • 95
    Name:
    BG NH2
    Description:
    BG NH2 2 mg
    Catalog Number:
    S9148S
    Price:
    400
    Category:
    Peptide Synthesis Reagents
    Size:
    2 mg
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    Structured Review

    New England Biolabs building block cbg nh2
    BG NH2
    BG NH2 2 mg
    https://www.bioz.com/result/building block cbg nh2/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    building block cbg nh2 - by Bioz Stars, 2021-06
    95/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Near-Infrared Fluorescence Imaging of Mammalian Cells and Xenograft Tumors with SNAP-Tag
    Article Snippet: Ethics statement All experimental procedures for the use of animals were previously reviewed and approved by the institutional animal care and use committee (IACUC) at the University of Nebraska-Lincoln (protocol #402), and all of the experiments were conducted in accordance with the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health. .. Chemicals and reagents The SNAPf -Beta-2 adrenergic receptor (SNAPf -ADRβ2) vector, amine-terminated building block BG-NH2, BG-782 (SNAP-Surface 782) and purified SNAPf -EGF protein were from New England Biolabs (Ipswich, MA). .. The IRDye 800CW and IRDye QC1 were from LI-COR Biosciences (Lincoln, NE).

    Blocking Assay:

    Article Title: Near-Infrared Fluorescence Imaging of Mammalian Cells and Xenograft Tumors with SNAP-Tag
    Article Snippet: Ethics statement All experimental procedures for the use of animals were previously reviewed and approved by the institutional animal care and use committee (IACUC) at the University of Nebraska-Lincoln (protocol #402), and all of the experiments were conducted in accordance with the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health. .. Chemicals and reagents The SNAPf -Beta-2 adrenergic receptor (SNAPf -ADRβ2) vector, amine-terminated building block BG-NH2, BG-782 (SNAP-Surface 782) and purified SNAPf -EGF protein were from New England Biolabs (Ipswich, MA). .. The IRDye 800CW and IRDye QC1 were from LI-COR Biosciences (Lincoln, NE).

    Article Title: Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging
    Article Snippet: Experimental Section Chemical methods: Commercially available compounds were used without further purification. .. All fluorogenic substrates for the labeling of SNAP-tag fusion proteins were prepared by reacting the building block CBG-NH2 (New England Biolabs) with commercially available N -hydroxysuccinimide esters of the corresponding fluorophores and amines of the corresponding quenchers. .. ATTO-488 NHS was purchased from ATTO-TEC GmbH (Siegen, Germany).

    Article Title: Three-color single molecule imaging shows WASP detachment from Arp2/3 complex triggers actin filament branch formation
    Article Snippet: Mass spectra were recorded by electrospray ionization (ESI) on an Agilent 6210 Time-of-Flight (TOF) or 6120 Quadrupole LC/MS system. .. BG-649 was prepared by reacting the building block BG-NH2 (New England Biolabs, Ipswich, MA) with the dye N -hydroxysuccinimide ester DY-649 NHS (Dyomics GmbH, Jena, Germany) as described previously ( ). ..

    Purification:

    Article Title: Near-Infrared Fluorescence Imaging of Mammalian Cells and Xenograft Tumors with SNAP-Tag
    Article Snippet: Ethics statement All experimental procedures for the use of animals were previously reviewed and approved by the institutional animal care and use committee (IACUC) at the University of Nebraska-Lincoln (protocol #402), and all of the experiments were conducted in accordance with the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health. .. Chemicals and reagents The SNAPf -Beta-2 adrenergic receptor (SNAPf -ADRβ2) vector, amine-terminated building block BG-NH2, BG-782 (SNAP-Surface 782) and purified SNAPf -EGF protein were from New England Biolabs (Ipswich, MA). .. The IRDye 800CW and IRDye QC1 were from LI-COR Biosciences (Lincoln, NE).

    Labeling:

    Article Title: Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging
    Article Snippet: Experimental Section Chemical methods: Commercially available compounds were used without further purification. .. All fluorogenic substrates for the labeling of SNAP-tag fusion proteins were prepared by reacting the building block CBG-NH2 (New England Biolabs) with commercially available N -hydroxysuccinimide esters of the corresponding fluorophores and amines of the corresponding quenchers. .. ATTO-488 NHS was purchased from ATTO-TEC GmbH (Siegen, Germany).

    Cross-linking Immunoprecipitation:

    Article Title: Two-color STED microscopy in living cells
    Article Snippet: Subsequently, the coding sequence of mature EGFR (GeneCopoeia) was amplified by PCR and subcloned into the plasmid pSNAPf using the unique SbfI and NotI sites creating pSNAPf -EGFR. .. 2.2 Chemical Synthesis of SNAP and CLIP-Tag STED Probes SNAP-tag and CLIP-tag fluorescent probes were synthesized from BG-NH2 [ , ] or BC-NH2 [ ] (New England Biolabs, Inc.) and commercially available N-hydroxysuccinimide (NHS) activated fluorescent dyes using standard procedures [ ]. .. DY-480XL NHS and DY-521XL NHS were purchased from Dyomics GmbH (Jena, Germany).

    Synthesized:

    Article Title: Two-color STED microscopy in living cells
    Article Snippet: Subsequently, the coding sequence of mature EGFR (GeneCopoeia) was amplified by PCR and subcloned into the plasmid pSNAPf using the unique SbfI and NotI sites creating pSNAPf -EGFR. .. 2.2 Chemical Synthesis of SNAP and CLIP-Tag STED Probes SNAP-tag and CLIP-tag fluorescent probes were synthesized from BG-NH2 [ , ] or BC-NH2 [ ] (New England Biolabs, Inc.) and commercially available N-hydroxysuccinimide (NHS) activated fluorescent dyes using standard procedures [ ]. .. DY-480XL NHS and DY-521XL NHS were purchased from Dyomics GmbH (Jena, Germany).

    Competitive Binding Assay:

    Article Title: Selection of a T7 promoter mutant with enhanced in vitro activity by a novel multi-copy bead display approach for in vitro evolution
    Article Snippet: Ten microlitres of the translation reaction was incubated with 10 µl of BG-agarose beads (Covalys) for 25 min at room temperature in the dark. .. For competition assay, 10 µl of the translation reaction was first incubated with 10 µl BG-NH2 (2 mg/ml) (New England Biolabs) for 25 min at room temperature in the dark. .. This reaction mixture was then incubated with 10 µl of BG-agarose beads for 25 min at room temperature in the dark.

    Incubation:

    Article Title: Selection of a T7 promoter mutant with enhanced in vitro activity by a novel multi-copy bead display approach for in vitro evolution
    Article Snippet: Ten microlitres of the translation reaction was incubated with 10 µl of BG-agarose beads (Covalys) for 25 min at room temperature in the dark. .. For competition assay, 10 µl of the translation reaction was first incubated with 10 µl BG-NH2 (2 mg/ml) (New England Biolabs) for 25 min at room temperature in the dark. .. This reaction mixture was then incubated with 10 µl of BG-agarose beads for 25 min at room temperature in the dark.

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  • 95
    New England Biolabs building block cbg nh2
    Building Block Cbg Nh2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/building block cbg nh2/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    building block cbg nh2 - by Bioz Stars, 2021-06
    95/100 stars
      Buy from Supplier

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