snap vista greentm substrate  (New England Biolabs)


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  • 94
    Name:
    SNAP Vista Green
    Description:
    SNAP Vista Green 50 nmol
    Catalog Number:
    s9147s
    Price:
    338
    Size:
    50 nmol
    Category:
    Fluorochromes
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    Structured Review

    New England Biolabs snap vista greentm substrate
    SNAP Vista Green
    SNAP Vista Green 50 nmol
    https://www.bioz.com/result/snap vista greentm substrate/product/New England Biolabs
    Average 94 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    snap vista greentm substrate - by Bioz Stars, 2020-05
    94/100 stars

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    Related Articles

    In Vivo:

    Article Title: Thermostability enhancement of the α-carbonic anhydrase from Sulfurihydrogenibium yellowstonense by using the anchoring-and-self-labelling-protein-tag system (ASLtag)
    Article Snippet: .. 2.4 Determination of the H5 activity by an in vitro and in vivo fluorescent assay Whole overnight inducted E. coli BL21(DE3) cells were collected and the expression of the H5 -derived fusion proteins was analysed by an in vitro assay with the fluorescent SNAP-Vista Green™ substrate (New England Biolabs, Ipswich, MA; hereinafter BG-FL), as previously described , . ..

    In Vitro:

    Article Title: Thermostability enhancement of the α-carbonic anhydrase from Sulfurihydrogenibium yellowstonense by using the anchoring-and-self-labelling-protein-tag system (ASLtag)
    Article Snippet: .. 2.4 Determination of the H5 activity by an in vitro and in vivo fluorescent assay Whole overnight inducted E. coli BL21(DE3) cells were collected and the expression of the H5 -derived fusion proteins was analysed by an in vitro assay with the fluorescent SNAP-Vista Green™ substrate (New England Biolabs, Ipswich, MA; hereinafter BG-FL), as previously described , . ..

    Fluorescence:

    Article Title: Biochemical and Structural Studies of the Mycobacterium tuberculosisO6-Methylguanine Methyltransferase and Mutated Variants
    Article Snippet: .. To measure the alkyltransferase activity of wild-type Mt OGT and relative mutants, a fluorescence assay using SNAP-Vista Green reagent (New England BioLabs) (here referred to as VG for brevity) was used, as previously described ( ) (see Fig. S2A in the supplemental material). .. Protein bands from SDS-PAGE were visualized by direct gel imaging (VersaDoc 4000; Bio-Rad), and their fluorescence intensities (FIs), corresponding to the reacted protein, were corrected for the actual amount of protein loaded into each lane by measuring the intensity of bands after Coomassie brilliant blue R250 staining.

    Article Title: Thermostability enhancement of the α-carbonic anhydrase from Sulfurihydrogenibium yellowstonense by using the anchoring-and-self-labelling-protein-tag system (ASLtag)
    Article Snippet: .. 2.4 Determination of the H5 activity by an in vitro and in vivo fluorescent assay Whole overnight inducted E. coli BL21(DE3) cells were collected and the expression of the H5 -derived fusion proteins was analysed by an in vitro assay with the fluorescent SNAP-Vista Green™ substrate (New England Biolabs, Ipswich, MA; hereinafter BG-FL), as previously described , . ..

    Incubation:

    Article Title: Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair
    Article Snippet: .. For the SNAP-Vista Green (NEB) substrate, the protein was incubated for 30 min on 30°C in the dark. .. After incubation, reactions (300 ng) were loaded on 6% SDS-PAGE gel and run at 80V for 160 min. Gels that were containing BG-Vista Green (NEB, SNAP-Vista Green), were imaged prior to silver staining.

    Article Title: RAL GTPases Drive Intestinal Stem Cell Function and Regeneration through Internalization of WNT Signalosomes
    Article Snippet: .. After additional 24h incubation, cells were washed once with PBS, and incubated with diluted SNAP-Vista® Green 1:1000 (New England Biolabs inc.) in PBS for 5 minutes in room temperature. .. To follow internalization, standard DMEM medium containing 10% serum was added to cells and incubated according to indicated time points.

    other:

    Article Title: In vivo and in vitro protein imaging in thermophilic archaea by exploiting a novel protein tag
    Article Snippet: Reagents All chemicals were purchased from Sigma-Aldrich; SNAP-Vista® Green substrate (referred to as BG-FL) was from New England Biolabs (Ipswich, MA).

    Activity Assay:

    Article Title: Biochemical and Structural Studies of the Mycobacterium tuberculosisO6-Methylguanine Methyltransferase and Mutated Variants
    Article Snippet: .. To measure the alkyltransferase activity of wild-type Mt OGT and relative mutants, a fluorescence assay using SNAP-Vista Green reagent (New England BioLabs) (here referred to as VG for brevity) was used, as previously described ( ) (see Fig. S2A in the supplemental material). .. Protein bands from SDS-PAGE were visualized by direct gel imaging (VersaDoc 4000; Bio-Rad), and their fluorescence intensities (FIs), corresponding to the reacted protein, were corrected for the actual amount of protein loaded into each lane by measuring the intensity of bands after Coomassie brilliant blue R250 staining.

    Article Title: Thermostability enhancement of the α-carbonic anhydrase from Sulfurihydrogenibium yellowstonense by using the anchoring-and-self-labelling-protein-tag system (ASLtag)
    Article Snippet: .. 2.4 Determination of the H5 activity by an in vitro and in vivo fluorescent assay Whole overnight inducted E. coli BL21(DE3) cells were collected and the expression of the H5 -derived fusion proteins was analysed by an in vitro assay with the fluorescent SNAP-Vista Green™ substrate (New England Biolabs, Ipswich, MA; hereinafter BG-FL), as previously described , . ..

    Expressing:

    Article Title: Thermostability enhancement of the α-carbonic anhydrase from Sulfurihydrogenibium yellowstonense by using the anchoring-and-self-labelling-protein-tag system (ASLtag)
    Article Snippet: .. 2.4 Determination of the H5 activity by an in vitro and in vivo fluorescent assay Whole overnight inducted E. coli BL21(DE3) cells were collected and the expression of the H5 -derived fusion proteins was analysed by an in vitro assay with the fluorescent SNAP-Vista Green™ substrate (New England Biolabs, Ipswich, MA; hereinafter BG-FL), as previously described , . ..

    Transformation Assay:

    Article Title: Activity and Regulation of Archaeal DNA Alkyltransferase
    Article Snippet: .. An appropriate volume of culture of freshly transformed E. coli ABLE C cells was washed twice in the same volume of 1× Fluo Reaction Buffer and finally resuspended in 50.0 μl of the same buffer supplemented with 5.0 μ m of the SNAP-Vista GreenTM substrate. .. After an incubation at 37 °C for 30 min, cells were washed twice with 1.0 ml of buffer and incubated for 30 min at 37 °C to allow the external diffusion of the unreacted substrate.

    Derivative Assay:

    Article Title: Thermostability enhancement of the α-carbonic anhydrase from Sulfurihydrogenibium yellowstonense by using the anchoring-and-self-labelling-protein-tag system (ASLtag)
    Article Snippet: .. 2.4 Determination of the H5 activity by an in vitro and in vivo fluorescent assay Whole overnight inducted E. coli BL21(DE3) cells were collected and the expression of the H5 -derived fusion proteins was analysed by an in vitro assay with the fluorescent SNAP-Vista Green™ substrate (New England Biolabs, Ipswich, MA; hereinafter BG-FL), as previously described , . ..

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  • 94
    New England Biolabs snap vista greentm substrate
    Snap Vista Greentm Substrate, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snap vista greentm substrate/product/New England Biolabs
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    snap vista greentm substrate - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

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