snap capture magnetic beads  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Name:
    SNAP Capture Magnetic Beads
    Description:
    SNAP Capture Magnetic Beads 2ml
    Catalog Number:
    S9145S
    Price:
    224
    Category:
    Protein Purification Kit Components
    Size:
    2 ml
    Buy from Supplier


    Structured Review

    New England Biolabs snap capture magnetic beads
    SNAP Capture Magnetic Beads
    SNAP Capture Magnetic Beads 2ml
    https://www.bioz.com/result/snap capture magnetic beads/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snap capture magnetic beads - by Bioz Stars, 2021-05
    93/100 stars

    Images

    1) Product Images from "Caenorhabditis elegans Galectins LEC-6 and LEC-10 Interact with Similar Glycoconjugates in the Intestine"

    Article Title: Caenorhabditis elegans Galectins LEC-6 and LEC-10 Interact with Similar Glycoconjugates in the Intestine

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M110.188581

    Expression of recombinant SNAP-fusion galectins. A , constructs for producing SNAP-LEC fusions and their uses in in situ detection of carbohydrates and in pull down of specific glycoproteins. B , expression and purification of SNAP-LEC-6. Lane 1 , molecular weight marker; lane 2 , lysate from E. coli induced for recombinant protein expression; lane 3 , lysate from noninduced cells; lanes 4–7 , consecutive fractions eluted from nickel resin. C , purified recombinant SNAP-LEC-6 labeled with infrared dye.
    Figure Legend Snippet: Expression of recombinant SNAP-fusion galectins. A , constructs for producing SNAP-LEC fusions and their uses in in situ detection of carbohydrates and in pull down of specific glycoproteins. B , expression and purification of SNAP-LEC-6. Lane 1 , molecular weight marker; lane 2 , lysate from E. coli induced for recombinant protein expression; lane 3 , lysate from noninduced cells; lanes 4–7 , consecutive fractions eluted from nickel resin. C , purified recombinant SNAP-LEC-6 labeled with infrared dye.

    Techniques Used: Expressing, Recombinant, Construct, In Situ, Purification, Molecular Weight, Marker, Labeling

    Labeled recombinant SNAP-LECs recognize target molecules located in diverse C. elegans tissues. A , LEC-1. Staining is found in the rectal region ( arrowheads ). B , LEC-6. Staining is most prominent along the intestinal lumen ( arrow ), the grinder of the pharynx ( star ), and the coelomocytes ( arrowhead ). C , LEC-9. Staining is more concentrated along the intestinal lumen ( arrow ) and shows significant but diffuse signal in most tissues. D , LEC-10. Staining is primarily along the lumen in the intestine ( arrow ). E , LEC-11. Staining is localized in the grinder ( arrowhead ) and the buccal cavity of the pharynx ( arrow ), and a diffuse signal is also present in most cells. F , CGL2. Staining is very similar to LEC-9 with concentrated signal localizing along the intestinal lumen with a diffuse signal in most tissues. G , diagram of tissues showing staining with recombinant galectins.
    Figure Legend Snippet: Labeled recombinant SNAP-LECs recognize target molecules located in diverse C. elegans tissues. A , LEC-1. Staining is found in the rectal region ( arrowheads ). B , LEC-6. Staining is most prominent along the intestinal lumen ( arrow ), the grinder of the pharynx ( star ), and the coelomocytes ( arrowhead ). C , LEC-9. Staining is more concentrated along the intestinal lumen ( arrow ) and shows significant but diffuse signal in most tissues. D , LEC-10. Staining is primarily along the lumen in the intestine ( arrow ). E , LEC-11. Staining is localized in the grinder ( arrowhead ) and the buccal cavity of the pharynx ( arrow ), and a diffuse signal is also present in most cells. F , CGL2. Staining is very similar to LEC-9 with concentrated signal localizing along the intestinal lumen with a diffuse signal in most tissues. G , diagram of tissues showing staining with recombinant galectins.

    Techniques Used: Labeling, Recombinant, Staining

    Related Articles

    Affinity Purification:

    Article Title: Biochemical Reduction of the Topology of the Diverse WDR76 Protein Interactome
    Article Snippet: .. Native Affinity Purification of WDR76 with associated proteins For large scale purifications, whole cell lysate or nuclear extracts were diluted in two volumes of lysis buffer (20 mM HEPES pH 7.5, 0.2% Triton X-100, 1.5 mM MgCl2, 0.42M NaCl, 10 mM KCl, 0.5 mM DTT) and incubated with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs). .. For small scale purifications, lysates were diluted in 700μl of TBS and incubated at 4μC with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs).

    Lysis:

    Article Title: Biochemical Reduction of the Topology of the Diverse WDR76 Protein Interactome
    Article Snippet: .. Native Affinity Purification of WDR76 with associated proteins For large scale purifications, whole cell lysate or nuclear extracts were diluted in two volumes of lysis buffer (20 mM HEPES pH 7.5, 0.2% Triton X-100, 1.5 mM MgCl2, 0.42M NaCl, 10 mM KCl, 0.5 mM DTT) and incubated with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs). .. For small scale purifications, lysates were diluted in 700μl of TBS and incubated at 4μC with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs).

    Incubation:

    Article Title: Biochemical Reduction of the Topology of the Diverse WDR76 Protein Interactome
    Article Snippet: .. Native Affinity Purification of WDR76 with associated proteins For large scale purifications, whole cell lysate or nuclear extracts were diluted in two volumes of lysis buffer (20 mM HEPES pH 7.5, 0.2% Triton X-100, 1.5 mM MgCl2, 0.42M NaCl, 10 mM KCl, 0.5 mM DTT) and incubated with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs). .. For small scale purifications, lysates were diluted in 700μl of TBS and incubated at 4μC with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs).

    Article Title: Lis1 has two opposing modes of regulating cytoplasmic dynein
    Article Snippet: Dynein was imaged every 1 sec for a total of 5 sec. Each sample was imaged at 4 different fields of view. .. Sixteen µL of magnetic SNAP-Capture beads (NEB) were incubated with increasing concentrations of SNAP-Lis1 (0–600 nM) in modified TEV buffer for 1 hour at room temperature with agitation. .. The supernatant was removed, the beads were washed with 1 ml of modified TEV buffer followed by 1 ml of TEV buffer supplemented with 1 mM DTT, 0.1% NP40, 2 mM MgCl2 , 1 mM ATP, and 1 mM NaVO4 .

    Article Title: Biochemical Reduction of the Topology of the Diverse WDR76 Protein Interactome
    Article Snippet: Native Affinity Purification of WDR76 with associated proteins For large scale purifications, whole cell lysate or nuclear extracts were diluted in two volumes of lysis buffer (20 mM HEPES pH 7.5, 0.2% Triton X-100, 1.5 mM MgCl2, 0.42M NaCl, 10 mM KCl, 0.5 mM DTT) and incubated with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs). .. For small scale purifications, lysates were diluted in 700μl of TBS and incubated at 4μC with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs). ..

    Article Title: Unusually efficient CUG initiation of an overlapping reading frame in POLG mRNA yields novel protein POLGARF
    Article Snippet: For SNAP pull down assay, transfected cells were lysed in 200 μL of PLB supplemented with 1 mM DTT. .. The lysates were mixed with 30 μL of SNAP-magnetic beads (NEB) and incubated for 1 h at 24 °C on a thermomixer (900 rpm). .. After incubation, the beads were washed twice with 1 mL of PBS supplemented with 1 mM dithiothreitol (DTT), and bound proteins were eluted by boiling with SDS gel loading buffer.

    Magnetic Beads:

    Article Title: Biochemical Reduction of the Topology of the Diverse WDR76 Protein Interactome
    Article Snippet: .. Native Affinity Purification of WDR76 with associated proteins For large scale purifications, whole cell lysate or nuclear extracts were diluted in two volumes of lysis buffer (20 mM HEPES pH 7.5, 0.2% Triton X-100, 1.5 mM MgCl2, 0.42M NaCl, 10 mM KCl, 0.5 mM DTT) and incubated with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs). .. For small scale purifications, lysates were diluted in 700μl of TBS and incubated at 4μC with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs).

    Article Title: Caenorhabditis elegans Galectins LEC-6 and LEC-10 Interact with Similar Glycoconjugates in the Intestine
    Article Snippet: .. While washing, the SNAP-Capture Magnetic Beads were separated from solution using a magnetic separation rack (NEB, S1506S). .. Proteins captured with the magnetic beads were eluted by boiling with 20 μl of SDS-loading buffer and separated by SDS-PAGE.

    Article Title: Driving integrative structural modeling with serial capture affinity purification
    Article Snippet: Critical Reagents.Magne HaloTag beads, sequencing-grade modified trypsin, rLys-C, and HaloTag ligands were purchased from Promega. .. All restriction endonucleases, SNAP-cell ligands, and SNAP-capture magnetic beads were purchased from New England BioLabs. .. PreScission protease was purchased from GE Healthcare Life Sciences.

    Article Title: Biochemical Reduction of the Topology of the Diverse WDR76 Protein Interactome
    Article Snippet: Native Affinity Purification of WDR76 with associated proteins For large scale purifications, whole cell lysate or nuclear extracts were diluted in two volumes of lysis buffer (20 mM HEPES pH 7.5, 0.2% Triton X-100, 1.5 mM MgCl2, 0.42M NaCl, 10 mM KCl, 0.5 mM DTT) and incubated with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs). .. For small scale purifications, lysates were diluted in 700μl of TBS and incubated at 4μC with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs). ..

    Article Title: SLALOM: A Simple and Rapid Method for Enzymatic Synthesis of CRISPR-Cas9 sgRNA Libraries
    Article Snippet: The sgRNA was then purified using either the RNA Clean & Concentrator-5 Kit (Zymo Research, Irvine, USA) or by phenol-chloroform extraction and ethanol precipitation. .. Capture beads were prepared by resuspending 50 μL of streptavidin magnetic beads (New England Biolabs, Ipswich, USA) in 20 μL containing 100 pmol of the biotinylated oligo and incubating at room temperature for 15 minutes. ..

    Modification:

    Article Title: Lis1 has two opposing modes of regulating cytoplasmic dynein
    Article Snippet: Dynein was imaged every 1 sec for a total of 5 sec. Each sample was imaged at 4 different fields of view. .. Sixteen µL of magnetic SNAP-Capture beads (NEB) were incubated with increasing concentrations of SNAP-Lis1 (0–600 nM) in modified TEV buffer for 1 hour at room temperature with agitation. .. The supernatant was removed, the beads were washed with 1 ml of modified TEV buffer followed by 1 ml of TEV buffer supplemented with 1 mM DTT, 0.1% NP40, 2 mM MgCl2 , 1 mM ATP, and 1 mM NaVO4 .

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    New England Biolabs snap capture magnetic beads
    Expression of recombinant <t>SNAP-fusion</t> galectins. A , constructs for producing SNAP-LEC fusions and their uses in in situ detection of carbohydrates and in pull down of specific glycoproteins. B , expression and purification of SNAP-LEC-6. Lane 1 , molecular weight marker; lane 2 , lysate from E. coli induced for recombinant protein expression; lane 3 , lysate from noninduced cells; lanes 4–7 , consecutive fractions eluted from nickel resin. C , purified recombinant SNAP-LEC-6 labeled with infrared dye.
    Snap Capture Magnetic Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snap capture magnetic beads/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snap capture magnetic beads - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    Expression of recombinant SNAP-fusion galectins. A , constructs for producing SNAP-LEC fusions and their uses in in situ detection of carbohydrates and in pull down of specific glycoproteins. B , expression and purification of SNAP-LEC-6. Lane 1 , molecular weight marker; lane 2 , lysate from E. coli induced for recombinant protein expression; lane 3 , lysate from noninduced cells; lanes 4–7 , consecutive fractions eluted from nickel resin. C , purified recombinant SNAP-LEC-6 labeled with infrared dye.

    Journal: The Journal of Biological Chemistry

    Article Title: Caenorhabditis elegans Galectins LEC-6 and LEC-10 Interact with Similar Glycoconjugates in the Intestine

    doi: 10.1074/jbc.M110.188581

    Figure Lengend Snippet: Expression of recombinant SNAP-fusion galectins. A , constructs for producing SNAP-LEC fusions and their uses in in situ detection of carbohydrates and in pull down of specific glycoproteins. B , expression and purification of SNAP-LEC-6. Lane 1 , molecular weight marker; lane 2 , lysate from E. coli induced for recombinant protein expression; lane 3 , lysate from noninduced cells; lanes 4–7 , consecutive fractions eluted from nickel resin. C , purified recombinant SNAP-LEC-6 labeled with infrared dye.

    Article Snippet: Pull-down Experiments with SNAP-galectin Fusions 100 μl of SNAP-capture magnetic beads (NEB S9145) (with a coupling capacity of 0.5 mg/ml for SNAP protein) were washed twice in immobilization buffer (50 mm Tris-Cl, pH 7.5, 100 mm NaCl, 1 mm DTT, and 0.1% Tween 20).

    Techniques: Expressing, Recombinant, Construct, In Situ, Purification, Molecular Weight, Marker, Labeling

    Labeled recombinant SNAP-LECs recognize target molecules located in diverse C. elegans tissues. A , LEC-1. Staining is found in the rectal region ( arrowheads ). B , LEC-6. Staining is most prominent along the intestinal lumen ( arrow ), the grinder of the pharynx ( star ), and the coelomocytes ( arrowhead ). C , LEC-9. Staining is more concentrated along the intestinal lumen ( arrow ) and shows significant but diffuse signal in most tissues. D , LEC-10. Staining is primarily along the lumen in the intestine ( arrow ). E , LEC-11. Staining is localized in the grinder ( arrowhead ) and the buccal cavity of the pharynx ( arrow ), and a diffuse signal is also present in most cells. F , CGL2. Staining is very similar to LEC-9 with concentrated signal localizing along the intestinal lumen with a diffuse signal in most tissues. G , diagram of tissues showing staining with recombinant galectins.

    Journal: The Journal of Biological Chemistry

    Article Title: Caenorhabditis elegans Galectins LEC-6 and LEC-10 Interact with Similar Glycoconjugates in the Intestine

    doi: 10.1074/jbc.M110.188581

    Figure Lengend Snippet: Labeled recombinant SNAP-LECs recognize target molecules located in diverse C. elegans tissues. A , LEC-1. Staining is found in the rectal region ( arrowheads ). B , LEC-6. Staining is most prominent along the intestinal lumen ( arrow ), the grinder of the pharynx ( star ), and the coelomocytes ( arrowhead ). C , LEC-9. Staining is more concentrated along the intestinal lumen ( arrow ) and shows significant but diffuse signal in most tissues. D , LEC-10. Staining is primarily along the lumen in the intestine ( arrow ). E , LEC-11. Staining is localized in the grinder ( arrowhead ) and the buccal cavity of the pharynx ( arrow ), and a diffuse signal is also present in most cells. F , CGL2. Staining is very similar to LEC-9 with concentrated signal localizing along the intestinal lumen with a diffuse signal in most tissues. G , diagram of tissues showing staining with recombinant galectins.

    Article Snippet: Pull-down Experiments with SNAP-galectin Fusions 100 μl of SNAP-capture magnetic beads (NEB S9145) (with a coupling capacity of 0.5 mg/ml for SNAP protein) were washed twice in immobilization buffer (50 mm Tris-Cl, pH 7.5, 100 mm NaCl, 1 mm DTT, and 0.1% Tween 20).

    Techniques: Labeling, Recombinant, Staining