snap galectin fusions  (New England Biolabs)


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    Name:
    SNAP Capture Magnetic Beads
    Description:
    SNAP Capture Magnetic Beads 2ml
    Catalog Number:
    s9145s
    Price:
    224
    Size:
    2 ml
    Category:
    Protein Purification Kit Components
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    Structured Review

    New England Biolabs snap galectin fusions
    SNAP Capture Magnetic Beads
    SNAP Capture Magnetic Beads 2ml
    https://www.bioz.com/result/snap galectin fusions/product/New England Biolabs
    Average 85 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    snap galectin fusions - by Bioz Stars, 2020-10
    85/100 stars

    Images

    1) Product Images from "Caenorhabditis elegans Galectins LEC-6 and LEC-10 Interact with Similar Glycoconjugates in the Intestine"

    Article Title: Caenorhabditis elegans Galectins LEC-6 and LEC-10 Interact with Similar Glycoconjugates in the Intestine

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M110.188581

    Expression of recombinant SNAP-fusion galectins. A , constructs for producing SNAP-LEC fusions and their uses in in situ detection of carbohydrates and in pull down of specific glycoproteins. B , expression and purification of SNAP-LEC-6. Lane 1 , molecular weight marker; lane 2 , lysate from E. coli induced for recombinant protein expression; lane 3 , lysate from noninduced cells; lanes 4–7 , consecutive fractions eluted from nickel resin. C , purified recombinant SNAP-LEC-6 labeled with infrared dye.
    Figure Legend Snippet: Expression of recombinant SNAP-fusion galectins. A , constructs for producing SNAP-LEC fusions and their uses in in situ detection of carbohydrates and in pull down of specific glycoproteins. B , expression and purification of SNAP-LEC-6. Lane 1 , molecular weight marker; lane 2 , lysate from E. coli induced for recombinant protein expression; lane 3 , lysate from noninduced cells; lanes 4–7 , consecutive fractions eluted from nickel resin. C , purified recombinant SNAP-LEC-6 labeled with infrared dye.

    Techniques Used: Expressing, Recombinant, Construct, In Situ, Purification, Molecular Weight, Marker, Labeling

    Labeled recombinant SNAP-LECs recognize target molecules located in diverse C. elegans tissues. A , LEC-1. Staining is found in the rectal region ( arrowheads ). B , LEC-6. Staining is most prominent along the intestinal lumen ( arrow ), the grinder of the pharynx ( star ), and the coelomocytes ( arrowhead ). C , LEC-9. Staining is more concentrated along the intestinal lumen ( arrow ) and shows significant but diffuse signal in most tissues. D , LEC-10. Staining is primarily along the lumen in the intestine ( arrow ). E , LEC-11. Staining is localized in the grinder ( arrowhead ) and the buccal cavity of the pharynx ( arrow ), and a diffuse signal is also present in most cells. F , CGL2. Staining is very similar to LEC-9 with concentrated signal localizing along the intestinal lumen with a diffuse signal in most tissues. G , diagram of tissues showing staining with recombinant galectins.
    Figure Legend Snippet: Labeled recombinant SNAP-LECs recognize target molecules located in diverse C. elegans tissues. A , LEC-1. Staining is found in the rectal region ( arrowheads ). B , LEC-6. Staining is most prominent along the intestinal lumen ( arrow ), the grinder of the pharynx ( star ), and the coelomocytes ( arrowhead ). C , LEC-9. Staining is more concentrated along the intestinal lumen ( arrow ) and shows significant but diffuse signal in most tissues. D , LEC-10. Staining is primarily along the lumen in the intestine ( arrow ). E , LEC-11. Staining is localized in the grinder ( arrowhead ) and the buccal cavity of the pharynx ( arrow ), and a diffuse signal is also present in most cells. F , CGL2. Staining is very similar to LEC-9 with concentrated signal localizing along the intestinal lumen with a diffuse signal in most tissues. G , diagram of tissues showing staining with recombinant galectins.

    Techniques Used: Labeling, Recombinant, Staining

    2) Product Images from "Caenorhabditis elegans Galectins LEC-6 and LEC-10 Interact with Similar Glycoconjugates in the Intestine"

    Article Title: Caenorhabditis elegans Galectins LEC-6 and LEC-10 Interact with Similar Glycoconjugates in the Intestine

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M110.188581

    Expression of recombinant SNAP-fusion galectins. A , constructs for producing SNAP-LEC fusions and their uses in in situ detection of carbohydrates and in pull down of specific glycoproteins. B , expression and purification of SNAP-LEC-6. Lane 1 , molecular weight marker; lane 2 , lysate from E. coli induced for recombinant protein expression; lane 3 , lysate from noninduced cells; lanes 4–7 , consecutive fractions eluted from nickel resin. C , purified recombinant SNAP-LEC-6 labeled with infrared dye.
    Figure Legend Snippet: Expression of recombinant SNAP-fusion galectins. A , constructs for producing SNAP-LEC fusions and their uses in in situ detection of carbohydrates and in pull down of specific glycoproteins. B , expression and purification of SNAP-LEC-6. Lane 1 , molecular weight marker; lane 2 , lysate from E. coli induced for recombinant protein expression; lane 3 , lysate from noninduced cells; lanes 4–7 , consecutive fractions eluted from nickel resin. C , purified recombinant SNAP-LEC-6 labeled with infrared dye.

    Techniques Used: Expressing, Recombinant, Construct, In Situ, Purification, Molecular Weight, Marker, Labeling

    Labeled recombinant SNAP-LECs recognize target molecules located in diverse C. elegans tissues. A , LEC-1. Staining is found in the rectal region ( arrowheads ). B , LEC-6. Staining is most prominent along the intestinal lumen ( arrow ), the grinder of the pharynx ( star ), and the coelomocytes ( arrowhead ). C , LEC-9. Staining is more concentrated along the intestinal lumen ( arrow ) and shows significant but diffuse signal in most tissues. D , LEC-10. Staining is primarily along the lumen in the intestine ( arrow ). E , LEC-11. Staining is localized in the grinder ( arrowhead ) and the buccal cavity of the pharynx ( arrow ), and a diffuse signal is also present in most cells. F , CGL2. Staining is very similar to LEC-9 with concentrated signal localizing along the intestinal lumen with a diffuse signal in most tissues. G , diagram of tissues showing staining with recombinant galectins.
    Figure Legend Snippet: Labeled recombinant SNAP-LECs recognize target molecules located in diverse C. elegans tissues. A , LEC-1. Staining is found in the rectal region ( arrowheads ). B , LEC-6. Staining is most prominent along the intestinal lumen ( arrow ), the grinder of the pharynx ( star ), and the coelomocytes ( arrowhead ). C , LEC-9. Staining is more concentrated along the intestinal lumen ( arrow ) and shows significant but diffuse signal in most tissues. D , LEC-10. Staining is primarily along the lumen in the intestine ( arrow ). E , LEC-11. Staining is localized in the grinder ( arrowhead ) and the buccal cavity of the pharynx ( arrow ), and a diffuse signal is also present in most cells. F , CGL2. Staining is very similar to LEC-9 with concentrated signal localizing along the intestinal lumen with a diffuse signal in most tissues. G , diagram of tissues showing staining with recombinant galectins.

    Techniques Used: Labeling, Recombinant, Staining

    Related Articles

    Transfection:

    Article Title: The SH3 regulatory domain of the hematopoietic cell kinase Hck binds ELMO via its polyproline motif
    Article Snippet: .. Lysates of HEK293T cells transiently transfected for the co-expression of the SH3 domain of Hck in the presence of wild type ELMO1–727 or the N-terminal deletion mutants ELMO532–727 and ELMO532–707 , were checked for correct protein expression by western-blot ( ) and subjected to pull-down experiments using ELMO1 as a bait, taking advantage of the specific and covalent binding of SNAP fusion constructs to the SNAP-capture magnetic beads (New England Biolabs, cf. .. The successful pull-down of SH3Hck was assessed by antibody labeling of the N-terminal CLIP tag of the construct.

    Magnetic Beads:

    Article Title: The SH3 regulatory domain of the hematopoietic cell kinase Hck binds ELMO via its polyproline motif
    Article Snippet: .. Lysates of HEK293T cells transiently transfected for the co-expression of the SH3 domain of Hck in the presence of wild type ELMO1–727 or the N-terminal deletion mutants ELMO532–727 and ELMO532–707 , were checked for correct protein expression by western-blot ( ) and subjected to pull-down experiments using ELMO1 as a bait, taking advantage of the specific and covalent binding of SNAP fusion constructs to the SNAP-capture magnetic beads (New England Biolabs, cf. .. The successful pull-down of SH3Hck was assessed by antibody labeling of the N-terminal CLIP tag of the construct.

    Article Title: Biochemical Reduction of the Topology of the Diverse WDR76 Protein Interactome
    Article Snippet: .. Native Affinity Purification of WDR76 with associated proteins For large scale purifications, whole cell lysate or nuclear extracts were diluted in two volumes of lysis buffer (20 mM HEPES pH 7.5, 0.2% Triton X-100, 1.5 mM MgCl2, 0.42M NaCl, 10 mM KCl, 0.5 mM DTT) and incubated with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs). .. For small scale purifications, lysates were diluted in 700μl of TBS and incubated at 4μC with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs).

    Article Title: The IDA3 adapter, required for intraflagellar transport of I1 dynein, is regulated by ciliary length
    Article Snippet: .. SNAP magnetic beads (Cat. No. S9145S; New England Biolabs) were prepared as follows: for each strain, 40 μl of the SNAP magnetic beads were spun down at room temperature using a bench-top minicentrifuge at top speed (14,000 × g ) for 1 min. .. The clarified supernatant was removed and the beads were incubated overnight in an end-over-end rocker at 4°C in a buffer containing 3% BSA, 10 mM HEPES, 5 mM MgSO4 , 1 mM DTT, 25 mM NaCl, and protease inhibitors at pH 7.4.

    Article Title: A ligand-based system for receptor-specific delivery of proteins
    Article Snippet: .. SNAP-capture magnetic beads (NEB #S9145S) were used to remove excess of free-ligand when this was present at the end of the process. .. In-vitro primary keratinocyte treatment with CLIP-Cre S-CROSS Cross-linked complexes or CLIP-Cre alone were diluted in-vitro in keratinocytes serum free media to the desired concentration (2 μM) in a final volume of 100 μl.

    Article Title: Caenorhabditis elegans Galectins LEC-6 and LEC-10 Interact with Similar Glycoconjugates in the Intestine
    Article Snippet: .. While washing, the SNAP-Capture Magnetic Beads were separated from solution using a magnetic separation rack (NEB, S1506S). .. Proteins captured with the magnetic beads were eluted by boiling with 20 μl of SDS-loading buffer and separated by SDS-PAGE.

    Construct:

    Article Title: The SH3 regulatory domain of the hematopoietic cell kinase Hck binds ELMO via its polyproline motif
    Article Snippet: .. Lysates of HEK293T cells transiently transfected for the co-expression of the SH3 domain of Hck in the presence of wild type ELMO1–727 or the N-terminal deletion mutants ELMO532–727 and ELMO532–707 , were checked for correct protein expression by western-blot ( ) and subjected to pull-down experiments using ELMO1 as a bait, taking advantage of the specific and covalent binding of SNAP fusion constructs to the SNAP-capture magnetic beads (New England Biolabs, cf. .. The successful pull-down of SH3Hck was assessed by antibody labeling of the N-terminal CLIP tag of the construct.

    Incubation:

    Article Title: Biochemical Reduction of the Topology of the Diverse WDR76 Protein Interactome
    Article Snippet: .. Native Affinity Purification of WDR76 with associated proteins For large scale purifications, whole cell lysate or nuclear extracts were diluted in two volumes of lysis buffer (20 mM HEPES pH 7.5, 0.2% Triton X-100, 1.5 mM MgCl2, 0.42M NaCl, 10 mM KCl, 0.5 mM DTT) and incubated with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs). .. For small scale purifications, lysates were diluted in 700μl of TBS and incubated at 4μC with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs).

    Article Title: Super-resolution imaging and tracking of protein–protein interactions in sub-diffraction cellular space
    Article Snippet: .. Cleared cell lysates were incubated for different time with 100 μl Snap capture beads (NEB) to pull down MreB interacting proteins. .. The specific interaction between MreB and EF-Tu was then verified by western blotting using an EF-Tu antibody (Hucult Biotech).

    Article Title: Lis1 has two opposing modes of regulating cytoplasmic dynein
    Article Snippet: .. Sixteen µL of magnetic SNAP-Capture beads (NEB) were incubated with increasing concentrations of SNAP-Lis1 (0–600 nM) in modified TEV buffer for 1 hour at room temperature with agitation. .. The supernatant was removed, the beads were washed with 1 ml of modified TEV buffer followed by 1 ml of TEV buffer supplemented with 1 mM DTT, 0.1% NP40, 2 mM MgCl2 , 1 mM ATP, and 1 mM NaVO4 .

    Expressing:

    Article Title: The SH3 regulatory domain of the hematopoietic cell kinase Hck binds ELMO via its polyproline motif
    Article Snippet: .. Lysates of HEK293T cells transiently transfected for the co-expression of the SH3 domain of Hck in the presence of wild type ELMO1–727 or the N-terminal deletion mutants ELMO532–727 and ELMO532–707 , were checked for correct protein expression by western-blot ( ) and subjected to pull-down experiments using ELMO1 as a bait, taking advantage of the specific and covalent binding of SNAP fusion constructs to the SNAP-capture magnetic beads (New England Biolabs, cf. .. The successful pull-down of SH3Hck was assessed by antibody labeling of the N-terminal CLIP tag of the construct.

    Western Blot:

    Article Title: The SH3 regulatory domain of the hematopoietic cell kinase Hck binds ELMO via its polyproline motif
    Article Snippet: .. Lysates of HEK293T cells transiently transfected for the co-expression of the SH3 domain of Hck in the presence of wild type ELMO1–727 or the N-terminal deletion mutants ELMO532–727 and ELMO532–707 , were checked for correct protein expression by western-blot ( ) and subjected to pull-down experiments using ELMO1 as a bait, taking advantage of the specific and covalent binding of SNAP fusion constructs to the SNAP-capture magnetic beads (New England Biolabs, cf. .. The successful pull-down of SH3Hck was assessed by antibody labeling of the N-terminal CLIP tag of the construct.

    Modification:

    Article Title: Lis1 has two opposing modes of regulating cytoplasmic dynein
    Article Snippet: .. Sixteen µL of magnetic SNAP-Capture beads (NEB) were incubated with increasing concentrations of SNAP-Lis1 (0–600 nM) in modified TEV buffer for 1 hour at room temperature with agitation. .. The supernatant was removed, the beads were washed with 1 ml of modified TEV buffer followed by 1 ml of TEV buffer supplemented with 1 mM DTT, 0.1% NP40, 2 mM MgCl2 , 1 mM ATP, and 1 mM NaVO4 .

    Lysis:

    Article Title: Biochemical Reduction of the Topology of the Diverse WDR76 Protein Interactome
    Article Snippet: .. Native Affinity Purification of WDR76 with associated proteins For large scale purifications, whole cell lysate or nuclear extracts were diluted in two volumes of lysis buffer (20 mM HEPES pH 7.5, 0.2% Triton X-100, 1.5 mM MgCl2, 0.42M NaCl, 10 mM KCl, 0.5 mM DTT) and incubated with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs). .. For small scale purifications, lysates were diluted in 700μl of TBS and incubated at 4μC with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs).

    Affinity Purification:

    Article Title: Biochemical Reduction of the Topology of the Diverse WDR76 Protein Interactome
    Article Snippet: .. Native Affinity Purification of WDR76 with associated proteins For large scale purifications, whole cell lysate or nuclear extracts were diluted in two volumes of lysis buffer (20 mM HEPES pH 7.5, 0.2% Triton X-100, 1.5 mM MgCl2, 0.42M NaCl, 10 mM KCl, 0.5 mM DTT) and incubated with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs). .. For small scale purifications, lysates were diluted in 700μl of TBS and incubated at 4μC with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs).

    Binding Assay:

    Article Title: The SH3 regulatory domain of the hematopoietic cell kinase Hck binds ELMO via its polyproline motif
    Article Snippet: .. Lysates of HEK293T cells transiently transfected for the co-expression of the SH3 domain of Hck in the presence of wild type ELMO1–727 or the N-terminal deletion mutants ELMO532–727 and ELMO532–707 , were checked for correct protein expression by western-blot ( ) and subjected to pull-down experiments using ELMO1 as a bait, taking advantage of the specific and covalent binding of SNAP fusion constructs to the SNAP-capture magnetic beads (New England Biolabs, cf. .. The successful pull-down of SH3Hck was assessed by antibody labeling of the N-terminal CLIP tag of the construct.

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  • 94
    New England Biolabs snap capture magnetic beads
    Expression of recombinant <t>SNAP-fusion</t> galectins. A , constructs for producing SNAP-LEC fusions and their uses in in situ detection of carbohydrates and in pull down of specific glycoproteins. B , expression and purification of SNAP-LEC-6. Lane 1 , molecular weight marker; lane 2 , lysate from E. coli induced for recombinant protein expression; lane 3 , lysate from noninduced cells; lanes 4–7 , consecutive fractions eluted from nickel resin. C , purified recombinant SNAP-LEC-6 labeled with infrared dye.
    Snap Capture Magnetic Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snap capture magnetic beads/product/New England Biolabs
    Average 94 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    snap capture magnetic beads - by Bioz Stars, 2020-10
    94/100 stars
      Buy from Supplier

    Image Search Results


    Expression of recombinant SNAP-fusion galectins. A , constructs for producing SNAP-LEC fusions and their uses in in situ detection of carbohydrates and in pull down of specific glycoproteins. B , expression and purification of SNAP-LEC-6. Lane 1 , molecular weight marker; lane 2 , lysate from E. coli induced for recombinant protein expression; lane 3 , lysate from noninduced cells; lanes 4–7 , consecutive fractions eluted from nickel resin. C , purified recombinant SNAP-LEC-6 labeled with infrared dye.

    Journal: The Journal of Biological Chemistry

    Article Title: Caenorhabditis elegans Galectins LEC-6 and LEC-10 Interact with Similar Glycoconjugates in the Intestine

    doi: 10.1074/jbc.M110.188581

    Figure Lengend Snippet: Expression of recombinant SNAP-fusion galectins. A , constructs for producing SNAP-LEC fusions and their uses in in situ detection of carbohydrates and in pull down of specific glycoproteins. B , expression and purification of SNAP-LEC-6. Lane 1 , molecular weight marker; lane 2 , lysate from E. coli induced for recombinant protein expression; lane 3 , lysate from noninduced cells; lanes 4–7 , consecutive fractions eluted from nickel resin. C , purified recombinant SNAP-LEC-6 labeled with infrared dye.

    Article Snippet: Pull-down Experiments with SNAP-galectin Fusions 100 μl of SNAP-capture magnetic beads (NEB S9145) (with a coupling capacity of 0.5 mg/ml for SNAP protein) were washed twice in immobilization buffer (50 mm Tris-Cl, pH 7.5, 100 mm NaCl, 1 mm DTT, and 0.1% Tween 20).

    Techniques: Expressing, Recombinant, Construct, In Situ, Purification, Molecular Weight, Marker, Labeling

    Labeled recombinant SNAP-LECs recognize target molecules located in diverse C. elegans tissues. A , LEC-1. Staining is found in the rectal region ( arrowheads ). B , LEC-6. Staining is most prominent along the intestinal lumen ( arrow ), the grinder of the pharynx ( star ), and the coelomocytes ( arrowhead ). C , LEC-9. Staining is more concentrated along the intestinal lumen ( arrow ) and shows significant but diffuse signal in most tissues. D , LEC-10. Staining is primarily along the lumen in the intestine ( arrow ). E , LEC-11. Staining is localized in the grinder ( arrowhead ) and the buccal cavity of the pharynx ( arrow ), and a diffuse signal is also present in most cells. F , CGL2. Staining is very similar to LEC-9 with concentrated signal localizing along the intestinal lumen with a diffuse signal in most tissues. G , diagram of tissues showing staining with recombinant galectins.

    Journal: The Journal of Biological Chemistry

    Article Title: Caenorhabditis elegans Galectins LEC-6 and LEC-10 Interact with Similar Glycoconjugates in the Intestine

    doi: 10.1074/jbc.M110.188581

    Figure Lengend Snippet: Labeled recombinant SNAP-LECs recognize target molecules located in diverse C. elegans tissues. A , LEC-1. Staining is found in the rectal region ( arrowheads ). B , LEC-6. Staining is most prominent along the intestinal lumen ( arrow ), the grinder of the pharynx ( star ), and the coelomocytes ( arrowhead ). C , LEC-9. Staining is more concentrated along the intestinal lumen ( arrow ) and shows significant but diffuse signal in most tissues. D , LEC-10. Staining is primarily along the lumen in the intestine ( arrow ). E , LEC-11. Staining is localized in the grinder ( arrowhead ) and the buccal cavity of the pharynx ( arrow ), and a diffuse signal is also present in most cells. F , CGL2. Staining is very similar to LEC-9 with concentrated signal localizing along the intestinal lumen with a diffuse signal in most tissues. G , diagram of tissues showing staining with recombinant galectins.

    Article Snippet: Pull-down Experiments with SNAP-galectin Fusions 100 μl of SNAP-capture magnetic beads (NEB S9145) (with a coupling capacity of 0.5 mg/ml for SNAP protein) were washed twice in immobilization buffer (50 mm Tris-Cl, pH 7.5, 100 mm NaCl, 1 mm DTT, and 0.1% Tween 20).

    Techniques: Labeling, Recombinant, Staining