snap surface alexa fluor 488  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    New England Biolabs snap surface alexa fluor 488
    Snap Surface Alexa Fluor 488, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snap surface alexa fluor 488/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snap surface alexa fluor 488 - by Bioz Stars, 2023-01
    95/100 stars

    Images

    snap surface alexa fluor 488  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    New England Biolabs snap surface alexa fluor 488
    Snap Surface Alexa Fluor 488, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snap surface alexa fluor 488/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snap surface alexa fluor 488 - by Bioz Stars, 2023-01
    95/100 stars

    Images

    anti mouse igg alexa fluor 488  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    New England Biolabs anti mouse igg alexa fluor 488
    Anti Mouse Igg Alexa Fluor 488, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse igg alexa fluor 488/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mouse igg alexa fluor 488 - by Bioz Stars, 2023-01
    95/100 stars

    Images

    alexafluor 488  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    New England Biolabs alexafluor 488
    Alexafluor 488, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexafluor 488/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alexafluor 488 - by Bioz Stars, 2023-01
    95/100 stars

    Images

    bg alexa 488  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    New England Biolabs bg alexa 488
    (A) BEAS-2B cells were preincubated with DMSO or 20 µM pitstop 2. Cells were then incubated with Alexa-594 Tfn and antibodies to MHCI in the presence of DMSO or the drugs for 30 min and then processed as described in . Lower panel represents the quantification of the integrated intensity of internalized proteins (Tfn and MHCI). The total integrated intensity of each cell was quantified as described in Materials and Methods. The mean fluorescence intensity, as arbitrary units, is plotted with standard deviation for 50 cells in both control and pitstop-treated cells. Both Tfn and MHCI internalization were inhibited at 20 µM pitstop 2 (p<0.001). (B) COS-7 cells were allowed to internalize <t>Alexa-488</t> Tfn and antibodies to MHCI in the absence or presence of pitstop 2, and quanitified as described in (A). Statistical analysis done for 50 cells with p<0.01 for Tfn inhibition and p<0.001 for MHCI inhibition. The images and quantification shown are from one experiment and similar to that observed in 2 additional experiments. Bars, 10 µm.
    Bg Alexa 488, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bg alexa 488/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bg alexa 488 - by Bioz Stars, 2023-01
    95/100 stars

    Images

    1) Product Images from "Pitstop 2 Is a Potent Inhibitor of Clathrin-Independent Endocytosis"

    Article Title: Pitstop 2 Is a Potent Inhibitor of Clathrin-Independent Endocytosis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0045799

    (A) BEAS-2B cells were preincubated with DMSO or 20 µM pitstop 2. Cells were then incubated with Alexa-594 Tfn and antibodies to MHCI in the presence of DMSO or the drugs for 30 min and then processed as described in . Lower panel represents the quantification of the integrated intensity of internalized proteins (Tfn and MHCI). The total integrated intensity of each cell was quantified as described in Materials and Methods. The mean fluorescence intensity, as arbitrary units, is plotted with standard deviation for 50 cells in both control and pitstop-treated cells. Both Tfn and MHCI internalization were inhibited at 20 µM pitstop 2 (p<0.001). (B) COS-7 cells were allowed to internalize Alexa-488 Tfn and antibodies to MHCI in the absence or presence of pitstop 2, and quanitified as described in (A). Statistical analysis done for 50 cells with p<0.01 for Tfn inhibition and p<0.001 for MHCI inhibition. The images and quantification shown are from one experiment and similar to that observed in 2 additional experiments. Bars, 10 µm.
    Figure Legend Snippet: (A) BEAS-2B cells were preincubated with DMSO or 20 µM pitstop 2. Cells were then incubated with Alexa-594 Tfn and antibodies to MHCI in the presence of DMSO or the drugs for 30 min and then processed as described in . Lower panel represents the quantification of the integrated intensity of internalized proteins (Tfn and MHCI). The total integrated intensity of each cell was quantified as described in Materials and Methods. The mean fluorescence intensity, as arbitrary units, is plotted with standard deviation for 50 cells in both control and pitstop-treated cells. Both Tfn and MHCI internalization were inhibited at 20 µM pitstop 2 (p<0.001). (B) COS-7 cells were allowed to internalize Alexa-488 Tfn and antibodies to MHCI in the absence or presence of pitstop 2, and quanitified as described in (A). Statistical analysis done for 50 cells with p<0.01 for Tfn inhibition and p<0.001 for MHCI inhibition. The images and quantification shown are from one experiment and similar to that observed in 2 additional experiments. Bars, 10 µm.

    Techniques Used: Incubation, Fluorescence, Standard Deviation, Inhibition

    snap surface alexa fluor 488  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    New England Biolabs snap surface alexa fluor 488
    Snap Surface Alexa Fluor 488, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snap surface alexa fluor 488/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snap surface alexa fluor 488 - by Bioz Stars, 2023-01
    95/100 stars

    Images

    snap surface alexa fluor 488 substrate  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    New England Biolabs snap surface alexa fluor 488 substrate
    Snap Surface Alexa Fluor 488 Substrate, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snap surface alexa fluor 488 substrate/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snap surface alexa fluor 488 substrate - by Bioz Stars, 2023-01
    95/100 stars

    Images

    snap surface alexa fluor 488  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    New England Biolabs snap surface alexa fluor 488
    ( A ) RNase activity of wild type (WT) and mutant (Q548X, H167Y, T592A) SAMHD1 towards indicated substrates. Buffer, negative control. Benzo, benzonase, positive control. First lane, ssRNA ladder; last lane, dsRNA ladder. ( B ) EU-Alexa <t>Fluor</t> <t>488</t> (green) and color-coded intensity of metabolically labeled nascent RNA in wild type (WT) and AGS fibroblasts. Scale bar, 5 μm. ( C ) Quantification of normalized RNA intensity. n = 51-82 cells. Box plots: center line, median; box, interquartile range; whiskers, 1.5x interquartile range; dots, outliers. Two-way ANOVA, ***, p<0.001. ( D ) Representative images of immune-stained phosphorylated RNA pol II, (pRNAPII; green) along with color-coded intensity in wild-type (WT) and AGS1 fibroblasts. Outlines of cell and nucleus are marked with white and yellow dashed lines, respectively. Scale bar, 5 μm. ( E ) Quantification of transcriptionally active RNA pol II. n = 11-19 cells. Box plots: center line, median; box, interquartile range; whiskers, 1.5x interquartile range; dots, outliers. Mann-Whitney U test. ***, p<0.001. ( F ) Total RNA levels in cytosol and nuclei from three different passages of AGS1 patient cells and three different wild type controls. Two-way ANOVA with Sidak’s multiple comparisons test. **, p<0.01. ( G ) Schematic of in cellulo RNA degradation assay. A 22 nt ssRNA probe labeled with 5’ FAM (fluorescence reporter, F) and 3’ TAMRA (quencher, Q) was microinjected into nuclei of HeLa cells expressing either control-shRNA or SAMHD1-shRNA and imaged after 10 min. ( H ) Representative images of intensity-coded FAM fluorescence at indicated time points of time series starting 10 min ( t = 0) after microinjection of FAM-TAMRA-labeled ssRNA oligonucleotide. Scale bar, 5 μm. ( I ) Quantification of FAM fluorescence in nuclei over time, where t = 0 is 10 min after microinjection of ssRNA probe. ( A-I ) Data are representative of at least three independent experiments.
    Snap Surface Alexa Fluor 488, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snap surface alexa fluor 488/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snap surface alexa fluor 488 - by Bioz Stars, 2023-01
    95/100 stars

    Images

    1) Product Images from "SAMHD1 controls innate immunity by regulating condensation of immunogenic self RNA"

    Article Title: SAMHD1 controls innate immunity by regulating condensation of immunogenic self RNA

    Journal: bioRxiv

    doi: 10.1101/2022.07.12.499661

    ( A ) RNase activity of wild type (WT) and mutant (Q548X, H167Y, T592A) SAMHD1 towards indicated substrates. Buffer, negative control. Benzo, benzonase, positive control. First lane, ssRNA ladder; last lane, dsRNA ladder. ( B ) EU-Alexa Fluor 488 (green) and color-coded intensity of metabolically labeled nascent RNA in wild type (WT) and AGS fibroblasts. Scale bar, 5 μm. ( C ) Quantification of normalized RNA intensity. n = 51-82 cells. Box plots: center line, median; box, interquartile range; whiskers, 1.5x interquartile range; dots, outliers. Two-way ANOVA, ***, p<0.001. ( D ) Representative images of immune-stained phosphorylated RNA pol II, (pRNAPII; green) along with color-coded intensity in wild-type (WT) and AGS1 fibroblasts. Outlines of cell and nucleus are marked with white and yellow dashed lines, respectively. Scale bar, 5 μm. ( E ) Quantification of transcriptionally active RNA pol II. n = 11-19 cells. Box plots: center line, median; box, interquartile range; whiskers, 1.5x interquartile range; dots, outliers. Mann-Whitney U test. ***, p<0.001. ( F ) Total RNA levels in cytosol and nuclei from three different passages of AGS1 patient cells and three different wild type controls. Two-way ANOVA with Sidak’s multiple comparisons test. **, p<0.01. ( G ) Schematic of in cellulo RNA degradation assay. A 22 nt ssRNA probe labeled with 5’ FAM (fluorescence reporter, F) and 3’ TAMRA (quencher, Q) was microinjected into nuclei of HeLa cells expressing either control-shRNA or SAMHD1-shRNA and imaged after 10 min. ( H ) Representative images of intensity-coded FAM fluorescence at indicated time points of time series starting 10 min ( t = 0) after microinjection of FAM-TAMRA-labeled ssRNA oligonucleotide. Scale bar, 5 μm. ( I ) Quantification of FAM fluorescence in nuclei over time, where t = 0 is 10 min after microinjection of ssRNA probe. ( A-I ) Data are representative of at least three independent experiments.
    Figure Legend Snippet: ( A ) RNase activity of wild type (WT) and mutant (Q548X, H167Y, T592A) SAMHD1 towards indicated substrates. Buffer, negative control. Benzo, benzonase, positive control. First lane, ssRNA ladder; last lane, dsRNA ladder. ( B ) EU-Alexa Fluor 488 (green) and color-coded intensity of metabolically labeled nascent RNA in wild type (WT) and AGS fibroblasts. Scale bar, 5 μm. ( C ) Quantification of normalized RNA intensity. n = 51-82 cells. Box plots: center line, median; box, interquartile range; whiskers, 1.5x interquartile range; dots, outliers. Two-way ANOVA, ***, p<0.001. ( D ) Representative images of immune-stained phosphorylated RNA pol II, (pRNAPII; green) along with color-coded intensity in wild-type (WT) and AGS1 fibroblasts. Outlines of cell and nucleus are marked with white and yellow dashed lines, respectively. Scale bar, 5 μm. ( E ) Quantification of transcriptionally active RNA pol II. n = 11-19 cells. Box plots: center line, median; box, interquartile range; whiskers, 1.5x interquartile range; dots, outliers. Mann-Whitney U test. ***, p<0.001. ( F ) Total RNA levels in cytosol and nuclei from three different passages of AGS1 patient cells and three different wild type controls. Two-way ANOVA with Sidak’s multiple comparisons test. **, p<0.01. ( G ) Schematic of in cellulo RNA degradation assay. A 22 nt ssRNA probe labeled with 5’ FAM (fluorescence reporter, F) and 3’ TAMRA (quencher, Q) was microinjected into nuclei of HeLa cells expressing either control-shRNA or SAMHD1-shRNA and imaged after 10 min. ( H ) Representative images of intensity-coded FAM fluorescence at indicated time points of time series starting 10 min ( t = 0) after microinjection of FAM-TAMRA-labeled ssRNA oligonucleotide. Scale bar, 5 μm. ( I ) Quantification of FAM fluorescence in nuclei over time, where t = 0 is 10 min after microinjection of ssRNA probe. ( A-I ) Data are representative of at least three independent experiments.

    Techniques Used: Activity Assay, Mutagenesis, Negative Control, Positive Control, Metabolic Labelling, Labeling, Staining, MANN-WHITNEY, Degradation Assay, Fluorescence, Expressing, shRNA

    snap surface alexa fluor 488  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    New England Biolabs snap surface alexa fluor 488
    Conjugation of scFv-SNAP fusion proteins with BG-modified agents. ( a ) Schematic diagram of conjugation. ScFv-SNAP fusion proteins conjugated with SNAP-Surface ® Alexa Fluor ® 488 and visualized with ChemiDoc XRS+ System ( b ) or SNAP-Surface ® Alexa Fluor ® 647 visualized with Odyssey DLx Imager ( c ). ( d ) Corresponding Coomassie brilliant blue stain of SDS-PAGE. M: Blue prestained protein standard broad range (11−250 kDa). Lanes 1–2: scFv-425-SNAP and scFv-EpCAM-SNAP were incubated with SNAP-Surface ® Alexa Fluor ® 488. Lanes 3–4: the same proteins were labeled with SNAP-Surface ® Alexa Fluor ® 647, and were post-incubated with SNAP-Surface ® Alexa Fluor ® 488. Lanes 5–6: the same proteins were labeled with BG-MMAE, and were post-incubated with SNAP-Surface ® Alexa Fluor ® 488.
    Snap Surface Alexa Fluor 488, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snap surface alexa fluor 488/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snap surface alexa fluor 488 - by Bioz Stars, 2023-01
    95/100 stars

    Images

    1) Product Images from "EpCAM- and EGFR-Specific Antibody Drug Conjugates for Triple-Negative Breast Cancer Treatment"

    Article Title: EpCAM- and EGFR-Specific Antibody Drug Conjugates for Triple-Negative Breast Cancer Treatment

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms23116122

    Conjugation of scFv-SNAP fusion proteins with BG-modified agents. ( a ) Schematic diagram of conjugation. ScFv-SNAP fusion proteins conjugated with SNAP-Surface ® Alexa Fluor ® 488 and visualized with ChemiDoc XRS+ System ( b ) or SNAP-Surface ® Alexa Fluor ® 647 visualized with Odyssey DLx Imager ( c ). ( d ) Corresponding Coomassie brilliant blue stain of SDS-PAGE. M: Blue prestained protein standard broad range (11−250 kDa). Lanes 1–2: scFv-425-SNAP and scFv-EpCAM-SNAP were incubated with SNAP-Surface ® Alexa Fluor ® 488. Lanes 3–4: the same proteins were labeled with SNAP-Surface ® Alexa Fluor ® 647, and were post-incubated with SNAP-Surface ® Alexa Fluor ® 488. Lanes 5–6: the same proteins were labeled with BG-MMAE, and were post-incubated with SNAP-Surface ® Alexa Fluor ® 488.
    Figure Legend Snippet: Conjugation of scFv-SNAP fusion proteins with BG-modified agents. ( a ) Schematic diagram of conjugation. ScFv-SNAP fusion proteins conjugated with SNAP-Surface ® Alexa Fluor ® 488 and visualized with ChemiDoc XRS+ System ( b ) or SNAP-Surface ® Alexa Fluor ® 647 visualized with Odyssey DLx Imager ( c ). ( d ) Corresponding Coomassie brilliant blue stain of SDS-PAGE. M: Blue prestained protein standard broad range (11−250 kDa). Lanes 1–2: scFv-425-SNAP and scFv-EpCAM-SNAP were incubated with SNAP-Surface ® Alexa Fluor ® 488. Lanes 3–4: the same proteins were labeled with SNAP-Surface ® Alexa Fluor ® 647, and were post-incubated with SNAP-Surface ® Alexa Fluor ® 488. Lanes 5–6: the same proteins were labeled with BG-MMAE, and were post-incubated with SNAP-Surface ® Alexa Fluor ® 488.

    Techniques Used: Conjugation Assay, Modification, Staining, SDS Page, Incubation, Labeling

    snap surface alexa fluor 488  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    New England Biolabs snap surface alexa fluor 488
    Snap Surface Alexa Fluor 488, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snap surface alexa fluor 488/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snap surface alexa fluor 488 - by Bioz Stars, 2023-01
    95/100 stars

    Images

    snap surface alexa fluor 488  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    New England Biolabs snap surface alexa fluor 488
    Snap Surface Alexa Fluor 488, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snap surface alexa fluor 488/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snap surface alexa fluor 488 - by Bioz Stars, 2023-01
    95/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    New England Biolabs snap surface alexa fluor 488
    Snap Surface Alexa Fluor 488, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snap surface alexa fluor 488/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snap surface alexa fluor 488 - by Bioz Stars, 2023-01
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs anti mouse igg alexa fluor 488
    Anti Mouse Igg Alexa Fluor 488, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse igg alexa fluor 488/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mouse igg alexa fluor 488 - by Bioz Stars, 2023-01
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs alexafluor 488
    Alexafluor 488, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexafluor 488/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alexafluor 488 - by Bioz Stars, 2023-01
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs bg alexa 488
    (A) BEAS-2B cells were preincubated with DMSO or 20 µM pitstop 2. Cells were then incubated with Alexa-594 Tfn and antibodies to MHCI in the presence of DMSO or the drugs for 30 min and then processed as described in . Lower panel represents the quantification of the integrated intensity of internalized proteins (Tfn and MHCI). The total integrated intensity of each cell was quantified as described in Materials and Methods. The mean fluorescence intensity, as arbitrary units, is plotted with standard deviation for 50 cells in both control and pitstop-treated cells. Both Tfn and MHCI internalization were inhibited at 20 µM pitstop 2 (p<0.001). (B) COS-7 cells were allowed to internalize <t>Alexa-488</t> Tfn and antibodies to MHCI in the absence or presence of pitstop 2, and quanitified as described in (A). Statistical analysis done for 50 cells with p<0.01 for Tfn inhibition and p<0.001 for MHCI inhibition. The images and quantification shown are from one experiment and similar to that observed in 2 additional experiments. Bars, 10 µm.
    Bg Alexa 488, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bg alexa 488/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bg alexa 488 - by Bioz Stars, 2023-01
    95/100 stars
      Buy from Supplier

    95
    New England Biolabs snap surface alexa fluor 488 substrate
    (A) BEAS-2B cells were preincubated with DMSO or 20 µM pitstop 2. Cells were then incubated with Alexa-594 Tfn and antibodies to MHCI in the presence of DMSO or the drugs for 30 min and then processed as described in . Lower panel represents the quantification of the integrated intensity of internalized proteins (Tfn and MHCI). The total integrated intensity of each cell was quantified as described in Materials and Methods. The mean fluorescence intensity, as arbitrary units, is plotted with standard deviation for 50 cells in both control and pitstop-treated cells. Both Tfn and MHCI internalization were inhibited at 20 µM pitstop 2 (p<0.001). (B) COS-7 cells were allowed to internalize <t>Alexa-488</t> Tfn and antibodies to MHCI in the absence or presence of pitstop 2, and quanitified as described in (A). Statistical analysis done for 50 cells with p<0.01 for Tfn inhibition and p<0.001 for MHCI inhibition. The images and quantification shown are from one experiment and similar to that observed in 2 additional experiments. Bars, 10 µm.
    Snap Surface Alexa Fluor 488 Substrate, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snap surface alexa fluor 488 substrate/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    snap surface alexa fluor 488 substrate - by Bioz Stars, 2023-01
    95/100 stars
      Buy from Supplier

    Image Search Results


    (A) BEAS-2B cells were preincubated with DMSO or 20 µM pitstop 2. Cells were then incubated with Alexa-594 Tfn and antibodies to MHCI in the presence of DMSO or the drugs for 30 min and then processed as described in . Lower panel represents the quantification of the integrated intensity of internalized proteins (Tfn and MHCI). The total integrated intensity of each cell was quantified as described in Materials and Methods. The mean fluorescence intensity, as arbitrary units, is plotted with standard deviation for 50 cells in both control and pitstop-treated cells. Both Tfn and MHCI internalization were inhibited at 20 µM pitstop 2 (p<0.001). (B) COS-7 cells were allowed to internalize Alexa-488 Tfn and antibodies to MHCI in the absence or presence of pitstop 2, and quanitified as described in (A). Statistical analysis done for 50 cells with p<0.01 for Tfn inhibition and p<0.001 for MHCI inhibition. The images and quantification shown are from one experiment and similar to that observed in 2 additional experiments. Bars, 10 µm.

    Journal: PLoS ONE

    Article Title: Pitstop 2 Is a Potent Inhibitor of Clathrin-Independent Endocytosis

    doi: 10.1371/journal.pone.0045799

    Figure Lengend Snippet: (A) BEAS-2B cells were preincubated with DMSO or 20 µM pitstop 2. Cells were then incubated with Alexa-594 Tfn and antibodies to MHCI in the presence of DMSO or the drugs for 30 min and then processed as described in . Lower panel represents the quantification of the integrated intensity of internalized proteins (Tfn and MHCI). The total integrated intensity of each cell was quantified as described in Materials and Methods. The mean fluorescence intensity, as arbitrary units, is plotted with standard deviation for 50 cells in both control and pitstop-treated cells. Both Tfn and MHCI internalization were inhibited at 20 µM pitstop 2 (p<0.001). (B) COS-7 cells were allowed to internalize Alexa-488 Tfn and antibodies to MHCI in the absence or presence of pitstop 2, and quanitified as described in (A). Statistical analysis done for 50 cells with p<0.01 for Tfn inhibition and p<0.001 for MHCI inhibition. The images and quantification shown are from one experiment and similar to that observed in 2 additional experiments. Bars, 10 µm.

    Article Snippet: BG-Alexa 488 is from New England Biolabs.

    Techniques: Incubation, Fluorescence, Standard Deviation, Inhibition