bg549 surface (New England Biolabs)
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Name:
SNAP Surface 549
Description:
SNAP Surface 549 50 nmol
Catalog Number:
s9112s
Price:
344
Size:
50 nmol
Category:
Fluorochromes
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Structured Review
New England Biolabs
bg549 surface
SNAP Surface 549 50 nmol
https://www.bioz.com/result/bg549 surface/product/New England Biolabs
Average 95 stars, based on 8 article reviews
Price from $9.99 to $1999.99
SNAP Surface 549 50 nmol
https://www.bioz.com/result/bg549 surface/product/New England Biolabs
Average 95 stars, based on 8 article reviews
Price from $9.99 to $1999.99
bg549 surface - by Bioz Stars,
2020-02
95/100 stars
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Related Articles
Electrophoresis:Article Title: A Single Mechanism of Biogenesis, Initiated and Directed by PIWI Proteins, Explains piRNA Production in Most Animals Article Snippet: .. SNAP-MIWI was labeled with Incubation:Article Title: Alternative transcription cycle for bacterial RNA polymerase Article Snippet: .. RNAP-SNAP was labeled with the DY-549 dye, yielding RNAP549 , as follows: 20 μL of 15 μM RNAP-SNAP was dialyzed into 3 L of labeling buffer (10 mM Tris-HCl, pH 8.0, 40 mM KCl, 5 mM MgCl2 , 20 μM ZnCl2 , and 1 mM dithiothreitol (DTT)) at 4 °C for 4 h. The resulting product (typically 50–100 μL of 5–20 μM of protein) was mixed with an equimolar amount of Article Title: Nerve growth factor–mediated photoablation of nociceptors reduces pain behavior in mice Article Snippet: For the labelling, 1-µM NGFR121W-SNAP was coupled with 3-µM Article Title: Synergistic assembly of human pre-spliceosomes across introns and exons Article Snippet: .. After centrifuging the lysate for 20 s at 12,000 × g , the nuclear pellet was resuspended in 450 µl Buffer C [20 mM Tris, 25% (v/v) glycerol, 0.42 M NaCl, 1.5 mM MgCl2 , 0.2 mM EDTA and 0.5 mM DTT, pH 7.9 at 4°C, supplemented with complete protease inhibitor cocktail (Roche, 04693159001)] and rapidly stirred in a 2 ml round-bottom microcentrifuge tube with a 12.7 × 3 mm stir bar for 30 min. After clarifying the lysate by centrifuging for 10 min at 12,000 × g , the Article Title: A ligand-based system for receptor-specific delivery of proteins Article Snippet: In-vitro labelling For keratinocyte labelling, IL-31K138A SNAP, NGFR121W SNAP or SNAP were coupled with an excess of Article Title: An engineered opsin monomer scrambles phospholipids Article Snippet: To the Neutravidin coated chambers, 10–20 nM of biotinylated monoclonal anti-FLAG antibody (Sigma, cat. no. F9291) was added and incubated for 30 mins at RT followed by washing with T50 buffer. .. The flow chamber was placed on an inverted TIRF microscope (Olympus IX73 with cellTIRF system) and purified Expressing:Article Title: Synergistic assembly of human pre-spliceosomes across introns and exons Article Snippet: Expression levels of the fSNAP fusion proteins were adjusted to endogenous level by inducing the U1-70K-fSNAP, U2B''-fSNAP, and Snu114-fSNAP Flp-In cell lines with 6 ng/ml, 3 ng/ml, and 3 ng/ml Doxycycline (BD Biosciences, 631311), respectively. .. After centrifuging the lysate for 20 s at 12,000 × g , the nuclear pellet was resuspended in 450 µl Buffer C [20 mM Tris, 25% (v/v) glycerol, 0.42 M NaCl, 1.5 mM MgCl2 , 0.2 mM EDTA and 0.5 mM DTT, pH 7.9 at 4°C, supplemented with complete protease inhibitor cocktail (Roche, 04693159001)] and rapidly stirred in a 2 ml round-bottom microcentrifuge tube with a 12.7 × 3 mm stir bar for 30 min. After clarifying the lysate by centrifuging for 10 min at 12,000 × g , the BIA-KA:Article Title: A Single Mechanism of Biogenesis, Initiated and Directed by PIWI Proteins, Explains piRNA Production in Most Animals Article Snippet: SNAP-MIWI was labeled with Western Blot:Article Title: A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression Article Snippet: Paragraph title: Western blot and SNAP-tag fluorescence labeling ... To detect the SNAP-tag, 10 μM Transfection:Article Title: Molecular details of dimerization kinetics reveal negligible populations of transient µ-opioid receptor homodimers at physiological concentrations Article Snippet: .. 24–36 hours after transfection cells were labeled with Article Title: PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways Article Snippet: .. 24 h post transfection, cell was stained with a 1:1 mixture of Article Title: Nerve growth factor–mediated photoablation of nociceptors reduces pain behavior in mice Article Snippet: In vitro experiments Hek293T cells were transfected with TrkA, TrkB, TrkC, and p75 plasmid using Lipofectamine 2000 (Thermo). .. For the labelling, 1-µM NGFR121W-SNAP was coupled with 3-µM Protease Inhibitor:Article Title: Synergistic assembly of human pre-spliceosomes across introns and exons Article Snippet: .. After centrifuging the lysate for 20 s at 12,000 × g , the nuclear pellet was resuspended in 450 µl Buffer C [20 mM Tris, 25% (v/v) glycerol, 0.42 M NaCl, 1.5 mM MgCl2 , 0.2 mM EDTA and 0.5 mM DTT, pH 7.9 at 4°C, supplemented with complete protease inhibitor cocktail (Roche, 04693159001)] and rapidly stirred in a 2 ml round-bottom microcentrifuge tube with a 12.7 × 3 mm stir bar for 30 min. After clarifying the lysate by centrifuging for 10 min at 12,000 × g , the Cell Culture:Article Title: Nerve growth factor–mediated photoablation of nociceptors reduces pain behavior in mice Article Snippet: For the labelling, 1-µM NGFR121W-SNAP was coupled with 3-µM Article Title: Agonist-induced dimer dissociation as a macromolecular step in G protein-coupled receptor signaling Article Snippet: Kinetic determination of mobile fractions of crosslinked V5-FZD6 -mCherry and FZD6 -GFP with WNT stimulation over time were performed in one cell culture well. .. The other:Article Title: Dynamic caveolae exclude bulk membrane proteins and are required for sorting of excess glycosphingolipids Article Snippet: The reagents used in this study are: cholera toxin B subunit conjugated to HRP (Molecular Probes, C3478) or alexa fluor 555 (Molecular Probes, C34776) or alexa fluor 647 (Molecular Probes, C34778); alexa fluor 647-conjugated human transferrin (Invitrogen, T23366); SNAP Cell surface Reagent 549 (New England Biolabs, S9112S); cyclohexamide (Santa-Cruz, sc-3508); Imaging:Article Title: Molecular details of dimerization kinetics reveal negligible populations of transient µ-opioid receptor homodimers at physiological concentrations Article Snippet: 24–36 hours after transfection cells were labeled with Article Title: Nerve growth factor–mediated photoablation of nociceptors reduces pain behavior in mice Article Snippet: .. For the labelling, 1-µM NGFR121W-SNAP was coupled with 3-µM Article Title: A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression Article Snippet: The signals were detected with a FluorChem HD2 imaging system (Alpha Innotech) and quantified with ImageQuant TL v2005 software. .. To detect the SNAP-tag, 10 μM Article Title: Agonist-induced dimer dissociation as a macromolecular step in G protein-coupled receptor signaling Article Snippet: Paragraph title: FRAP and cellular imaging ... The Protein Concentration:Article Title: Comprehensive and quantitative mapping of RNA–protein interactions across a transcribed eukaryotic genome Article Snippet: Protein concentration was measured both by Bradford dye reagent and A 280 absorbance. .. Subsequently, Vts1(442–523)-SNAP-His6 was fluorescently labeled using Staining:Article Title: PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways Article Snippet: .. 24 h post transfection, cell was stained with a 1:1 mixture of In Vivo:Article Title: Single-molecule Studies of Origin Licensing Reveal Mechanisms Ensuring Bidirectional Helicase Loading Article Snippet: The Ubiquitin ( in vivo ) and GST-SUMO (using Ulp1 protease) fusions were removed to reveal three N-terminal glycines required for sortase labeling. .. Fluorescence:Article Title: A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression Article Snippet: Paragraph title: Western blot and SNAP-tag fluorescence labeling ... To detect the SNAP-tag, 10 μM Article Title: Agonist-induced dimer dissociation as a macromolecular step in G protein-coupled receptor signaling Article Snippet: The mobile fraction was defined by the average of the fluorescence recovery assessed between time 85–101 s (including pre-bleach measurements). .. The Mutagenesis:Article Title: PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways Article Snippet: For , SNAP-tagged full length CD80 (SNAP–CD80), CD80 mutant (SNAP–CD80 I92R) or CD86 (SNAP–CD86) was transfected to either HEK293T cells or CLIP–PD-L1 transduced HEK293T cells using polyethylenimine. .. 24 h post transfection, cell was stained with a 1:1 mixture of Flow Cytometry:Article Title: Comprehensive and quantitative mapping of RNA–protein interactions across a transcribed eukaryotic genome Article Snippet: The dialysate was then mixed for 1 h with 2 mL of a 50% (vol/vol) slurry of SP-Sepharose resin (GE) that had been equilibrated in buffer C. The resin (1 mL) was then poured into a column and Vts1(442–523)-SNAP-His6 was recovered as a flow-through and subsequently concentrated by centrifugal ultrafiltration ( ). .. Subsequently, Vts1(442–523)-SNAP-His6 was fluorescently labeled using Article Title: An engineered opsin monomer scrambles phospholipids Article Snippet: .. The flow chamber was placed on an inverted TIRF microscope (Olympus IX73 with cellTIRF system) and purified Microscopy:Article Title: Molecular details of dimerization kinetics reveal negligible populations of transient µ-opioid receptor homodimers at physiological concentrations Article Snippet: Paragraph title: Microscope sample preparation and SNAP-labeling ... 24–36 hours after transfection cells were labeled with Article Title: PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways Article Snippet: Images were acquired with an Olympus FV1000 confocal microscope by exciting CS547 (energy donor) at 543 nm and SSAF647 (energy acceptor) at 635 nm. .. 24 h post transfection, cell was stained with a 1:1 mixture of Article Title: An engineered opsin monomer scrambles phospholipids Article Snippet: .. The flow chamber was placed on an inverted TIRF microscope (Olympus IX73 with cellTIRF system) and purified Purification:Article Title: Alternative transcription cycle for bacterial RNA polymerase Article Snippet: Proteins E. coli core RNAP (αββ′ω) with a SNAP tag on the c-terminus of β´ (RNAP-SNAP) and wild-type σ70 protein were expressed and purified . .. RNAP-SNAP was labeled with the DY-549 dye, yielding RNAP549 , as follows: 20 μL of 15 μM RNAP-SNAP was dialyzed into 3 L of labeling buffer (10 mM Tris-HCl, pH 8.0, 40 mM KCl, 5 mM MgCl2 , 20 μM ZnCl2 , and 1 mM dithiothreitol (DTT)) at 4 °C for 4 h. The resulting product (typically 50–100 μL of 5–20 μM of protein) was mixed with an equimolar amount of Article Title: A Single Mechanism of Biogenesis, Initiated and Directed by PIWI Proteins, Explains piRNA Production in Most Animals Article Snippet: SNAP-MIWI was labeled with Article Title: Single-molecule Studies of Origin Licensing Reveal Mechanisms Ensuring Bidirectional Helicase Loading Article Snippet: .. Article Title: An engineered opsin monomer scrambles phospholipids Article Snippet: .. The flow chamber was placed on an inverted TIRF microscope (Olympus IX73 with cellTIRF system) and purified Article Title: Quantitative analysis of RNA-protein interactions on a massively parallel array for mapping biophysical and evolutionary landscapes Article Snippet: .. Labeling MS2 Coat Protein with SNAPtag Substrate 5 μM purified SNAPtag-MS2 was labeled with Protein Purification:Article Title: Comprehensive and quantitative mapping of RNA–protein interactions across a transcribed eukaryotic genome Article Snippet: Paragraph title: Vts1 Protein Purification, Labeling, and Quantification. ... Subsequently, Vts1(442–523)-SNAP-His6 was fluorescently labeled using Article Title: Single-molecule Studies of Origin Licensing Reveal Mechanisms Ensuring Bidirectional Helicase Loading Article Snippet: Paragraph title: Protein Purification and Labeling ... Labeling:Article Title: Molecular details of dimerization kinetics reveal negligible populations of transient µ-opioid receptor homodimers at physiological concentrations Article Snippet: .. 24–36 hours after transfection cells were labeled with Article Title: Alternative transcription cycle for bacterial RNA polymerase Article Snippet: .. RNAP-SNAP was labeled with the DY-549 dye, yielding RNAP549 , as follows: 20 μL of 15 μM RNAP-SNAP was dialyzed into 3 L of labeling buffer (10 mM Tris-HCl, pH 8.0, 40 mM KCl, 5 mM MgCl2 , 20 μM ZnCl2 , and 1 mM dithiothreitol (DTT)) at 4 °C for 4 h. The resulting product (typically 50–100 μL of 5–20 μM of protein) was mixed with an equimolar amount of Article Title: PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways Article Snippet: Labeled cells were then fixed with 4% paraformaldehyde (PFA, Fisher Scientific, 50980494) and used for the FRET assay. .. 24 h post transfection, cell was stained with a 1:1 mixture of Article Title: Comprehensive and quantitative mapping of RNA–protein interactions across a transcribed eukaryotic genome Article Snippet: .. Subsequently, Vts1(442–523)-SNAP-His6 was fluorescently labeled using Article Title: A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression Article Snippet: Paragraph title: Western blot and SNAP-tag fluorescence labeling ... To detect the SNAP-tag, 10 μM Article Title: Ribonucleoprotein purification and characterization using RNA Mango Article Snippet: Paragraph title: SNAP labeling of yeast whole-cell extract ... Yeast whole-cell extracts (500 µL) were first fluorescently tagged with the Article Title: A Single Mechanism of Biogenesis, Initiated and Directed by PIWI Proteins, Explains piRNA Production in Most Animals Article Snippet: .. SNAP-MIWI was labeled with Article Title: Synergistic assembly of human pre-spliceosomes across introns and exons Article Snippet: After centrifuging the lysate for 20 s at 12,000 × g , the nuclear pellet was resuspended in 450 µl Buffer C [20 mM Tris, 25% (v/v) glycerol, 0.42 M NaCl, 1.5 mM MgCl2 , 0.2 mM EDTA and 0.5 mM DTT, pH 7.9 at 4°C, supplemented with complete protease inhibitor cocktail (Roche, 04693159001)] and rapidly stirred in a 2 ml round-bottom microcentrifuge tube with a 12.7 × 3 mm stir bar for 30 min. After clarifying the lysate by centrifuging for 10 min at 12,000 × g , the Article Title: Single-molecule Studies of Origin Licensing Reveal Mechanisms Ensuring Bidirectional Helicase Loading Article Snippet: Paragraph title: Protein Purification and Labeling ... Article Title: An engineered opsin monomer scrambles phospholipids Article Snippet: .. The flow chamber was placed on an inverted TIRF microscope (Olympus IX73 with cellTIRF system) and purified Article Title: Quantitative analysis of RNA-protein interactions on a massively parallel array for mapping biophysical and evolutionary landscapes Article Snippet: .. Labeling MS2 Coat Protein with SNAPtag Substrate 5 μM purified SNAPtag-MS2 was labeled with Confocal Microscopy:Article Title: PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways Article Snippet: Paragraph title: Confocal Microscopy Based FRET Assay with HEK293T Cells ... 24 h post transfection, cell was stained with a 1:1 mixture of FACS:Article Title: A Single Mechanism of Biogenesis, Initiated and Directed by PIWI Proteins, Explains piRNA Production in Most Animals Article Snippet: SNAP-MIWI was labeled with Mouse Assay:Article Title: A ligand-based system for receptor-specific delivery of proteins Article Snippet: In-vitro labelling For keratinocyte labelling, IL-31K138A SNAP, NGFR121W SNAP or SNAP were coupled with an excess of SDS Page:Article Title: Comprehensive and quantitative mapping of RNA–protein interactions across a transcribed eukaryotic genome Article Snippet: The elution profile was monitored by SDS/PAGE. .. Subsequently, Vts1(442–523)-SNAP-His6 was fluorescently labeled using Plasmid Preparation:Article Title: Nerve growth factor–mediated photoablation of nociceptors reduces pain behavior in mice Article Snippet: In vitro experiments Hek293T cells were transfected with TrkA, TrkB, TrkC, and p75 plasmid using Lipofectamine 2000 (Thermo). .. For the labelling, 1-µM NGFR121W-SNAP was coupled with 3-µM Software:Article Title: A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression Article Snippet: The signals were detected with a FluorChem HD2 imaging system (Alpha Innotech) and quantified with ImageQuant TL v2005 software. .. To detect the SNAP-tag, 10 μM Article Title: An engineered opsin monomer scrambles phospholipids Article Snippet: The flow chamber was placed on an inverted TIRF microscope (Olympus IX73 with cellTIRF system) and purified Sample Prep:Article Title: Molecular details of dimerization kinetics reveal negligible populations of transient µ-opioid receptor homodimers at physiological concentrations Article Snippet: Paragraph title: Microscope sample preparation and SNAP-labeling ... 24–36 hours after transfection cells were labeled with In Vitro:Article Title: Nerve growth factor–mediated photoablation of nociceptors reduces pain behavior in mice Article Snippet: Paragraph title: 3.4. In vitro experiments ... For the labelling, 1-µM NGFR121W-SNAP was coupled with 3-µM Article Title: A ligand-based system for receptor-specific delivery of proteins Article Snippet: .. In-vitro labelling For keratinocyte labelling, IL-31K138A SNAP, NGFR121W SNAP or SNAP were coupled with an excess of Produced:Article Title: A Single Mechanism of Biogenesis, Initiated and Directed by PIWI Proteins, Explains piRNA Production in Most Animals Article Snippet: SNAP-tagged Mus musculus PIWIL1 (SNAP-MIWI) and PIWIL2 (SNAP-MILI) were produced in HEK293T cells from a lentiviral-transduced transgene (lentivirus backbone was a gift from Greiner Lab). .. SNAP-MIWI was labeled with Concentration Assay:Article Title: Comprehensive and quantitative mapping of RNA–protein interactions across a transcribed eukaryotic genome Article Snippet: Subsequently, Vts1(442–523)-SNAP-His6 was fluorescently labeled using Article Title: Ribonucleoprotein purification and characterization using RNA Mango Article Snippet: .. Yeast whole-cell extracts (500 µL) were first fluorescently tagged with the Article Title: A Single Mechanism of Biogenesis, Initiated and Directed by PIWI Proteins, Explains piRNA Production in Most Animals Article Snippet: SNAP-MIWI was labeled with Article Title: Synergistic assembly of human pre-spliceosomes across introns and exons Article Snippet: .. After centrifuging the lysate for 20 s at 12,000 × g , the nuclear pellet was resuspended in 450 µl Buffer C [20 mM Tris, 25% (v/v) glycerol, 0.42 M NaCl, 1.5 mM MgCl2 , 0.2 mM EDTA and 0.5 mM DTT, pH 7.9 at 4°C, supplemented with complete protease inhibitor cocktail (Roche, 04693159001)] and rapidly stirred in a 2 ml round-bottom microcentrifuge tube with a 12.7 × 3 mm stir bar for 30 min. After clarifying the lysate by centrifuging for 10 min at 12,000 × g , the Lysis:Article Title: A Single Mechanism of Biogenesis, Initiated and Directed by PIWI Proteins, Explains piRNA Production in Most Animals Article Snippet: Cells, washed twice with PBS, were homogenized in lysis buffer (30 mM HEPES-KOH pH 7.5, 100 mM potassium acetate, 3.5 mM magnesium acetate, 1 mM dithiothreitol, 20% (w/v) glycerol, 0.1% (v/v) Triton X-100, 1 mM 4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride, 0.3 µM Aprotinin, 40 µM Bestatin, 10 µM E-64, 10 µM Leupeptin). .. SNAP-MIWI was labeled with Cross-linking Immunoprecipitation:Article Title: PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways Article Snippet: For , SNAP-tagged full length CD80 (SNAP–CD80), CD80 mutant (SNAP–CD80 I92R) or CD86 (SNAP–CD86) was transfected to either HEK293T cells or CLIP–PD-L1 transduced HEK293T cells using polyethylenimine. .. 24 h post transfection, cell was stained with a 1:1 mixture of |