snap cell fluorescein  (New England Biolabs)


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    Name:
    SNAP Cell Fluorescein
    Description:
    SNAP Cell Fluorescein 50 nmol
    Catalog Number:
    s9107s
    Price:
    307
    Size:
    50 nmol
    Category:
    Fluorochromes
    Buy from Supplier


    Structured Review

    New England Biolabs snap cell fluorescein
    SNAP Cell Fluorescein
    SNAP Cell Fluorescein 50 nmol
    https://www.bioz.com/result/snap cell fluorescein/product/New England Biolabs
    Average 94 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    snap cell fluorescein - by Bioz Stars, 2020-07
    94/100 stars

    Images

    1) Product Images from "Cytosolic chaperones mediate quality control of higher-order septin assembly in budding yeast"

    Article Title: Cytosolic chaperones mediate quality control of higher-order septin assembly in budding yeast

    Journal: Molecular Biology of the Cell

    doi: 10.1091/mbc.E14-11-1531

    Mutant septins evade exclusion by quality control if assembled into hetero-octamers before introduction of the wild-type allele. Top, fusion of the SNAP-Tag (SNAP) to the C- terminus of Cdc10(D182N) allows covalent fluorescence pulse labeling of a pool of Cdc10(D182N)-SNAP molecules using BG-DAF. The localization of the labeled mutant Cdc10-SNAP molecules after the introduction of wild-type, untagged Cdc10 via mating was monitored by fluorescence microscopy. Bottom, micrographs taken of budded zygotes generated by mating BG-DAF–labeled cells of strain JTY5169 with CDC10 + cells of strain BY4742. Arrowheads, labeled Cdc10(D182N)-GFP in septin rings at zygote bud necks. Asterisks, labeled Cdc10(D182N)-GFP in septin rings in nonzygote cells. Right, quantification of bud neck fluorescence in budded zygotes, as in Figure 1, B–E .
    Figure Legend Snippet: Mutant septins evade exclusion by quality control if assembled into hetero-octamers before introduction of the wild-type allele. Top, fusion of the SNAP-Tag (SNAP) to the C- terminus of Cdc10(D182N) allows covalent fluorescence pulse labeling of a pool of Cdc10(D182N)-SNAP molecules using BG-DAF. The localization of the labeled mutant Cdc10-SNAP molecules after the introduction of wild-type, untagged Cdc10 via mating was monitored by fluorescence microscopy. Bottom, micrographs taken of budded zygotes generated by mating BG-DAF–labeled cells of strain JTY5169 with CDC10 + cells of strain BY4742. Arrowheads, labeled Cdc10(D182N)-GFP in septin rings at zygote bud necks. Asterisks, labeled Cdc10(D182N)-GFP in septin rings in nonzygote cells. Right, quantification of bud neck fluorescence in budded zygotes, as in Figure 1, B–E .

    Techniques Used: Mutagenesis, Fluorescence, Labeling, Microscopy, Generated

    Related Articles

    DNA Extraction:

    Article Title: Prdm8 regulates pMN progenitor specification for motor neuron and oligodendrocyte fates by modulating Shh signaling response
    Article Snippet: .. The following day injected embryos were assayed for sgRNA activity by DNA extraction and two rounds of PCR amplification, first to amplify the prdm8 CRIPSR target with gene specific primers containing a M13F extension to the 5’ end of the forward primer (5’TGTAAAACGACGGCCAGT3’) and a second to add a fluorescein tag to the 5’ end of the amplified region (Table 2). .. The fluorescein tagged PCR product was analyzed using capillary gel electrophoresis to detect product length.

    Amplification:

    Article Title: Prdm8 regulates pMN progenitor specification for motor neuron and oligodendrocyte fates by modulating Shh signaling response
    Article Snippet: .. The following day injected embryos were assayed for sgRNA activity by DNA extraction and two rounds of PCR amplification, first to amplify the prdm8 CRIPSR target with gene specific primers containing a M13F extension to the 5’ end of the forward primer (5’TGTAAAACGACGGCCAGT3’) and a second to add a fluorescein tag to the 5’ end of the amplified region (Table 2). .. The fluorescein tagged PCR product was analyzed using capillary gel electrophoresis to detect product length.

    Labeling:

    Article Title: Subcellular drug targeting illuminates local kinase action
    Article Snippet: .. Cellular labeling : SNAP expressing (dox-induced) cells were treated with SNAP-Cell Fluorescein or SNAP-Cell 647-SiR (NEB) for 30 min in serum free DMEM at 37°C and 5% CO2. .. Cells were washed one time and incubated in fresh serum free DMEM for 30 min.

    Article Title: Kinetic pathway of 40S ribosomal subunit recruitment to hepatitis C virus internal ribosome entry site
    Article Snippet: .. For live cell fluorescent labeling, cells were incubated for 30 min at 37 °C in IMDM containing 5 μM SNAP-fluorescein (S9107S; New England Biolabs). ..

    Article Title: Analysis of Global Sumoylation Changes Occurring during Keratinocyte Differentiation
    Article Snippet: .. Fluorescent images were captured after cells were labeled with SNAP-Cell Fluorescein (NEB) per the manufacturer's instructions. .. The cells were exposed for 20 ms and images were captured using the Olympus IX81 microscope with the DP71 camera and FITC filter set at 20× magnification.

    Incubation:

    Article Title: Kinetic pathway of 40S ribosomal subunit recruitment to hepatitis C virus internal ribosome entry site
    Article Snippet: .. For live cell fluorescent labeling, cells were incubated for 30 min at 37 °C in IMDM containing 5 μM SNAP-fluorescein (S9107S; New England Biolabs). ..

    Article Title: The Giardia cell cycle progresses independently of the anaphase-promoting complex
    Article Snippet: .. Cells expressing SNAP-tagged cyclin B (∼1×108 cells) were iced and harvested, then incubated in 1 ml of complete media containing 5 µM of SNAP-fluorescein (New England Biolabs) for 3 hours at 37°C. .. Cells were chilled on ice for 20 minutes, harvested by centrifugation and resuspended in fresh media containing 10 µM SNAP-Block reagent (New England Biolabs) to start the chase.

    other:

    Article Title: Exploiting Ligand-Protein Conjugates to Monitor Ligand-Receptor Interactions
    Article Snippet: SNAP-Cell Fluorescein (BG-fluorescein), SNAP-Cell TMR-Star (BG-tetramethylrhodamine), SNAP-Surface 647 (BG-647) were provided by NEB.

    Activity Assay:

    Article Title: Prdm8 regulates pMN progenitor specification for motor neuron and oligodendrocyte fates by modulating Shh signaling response
    Article Snippet: .. The following day injected embryos were assayed for sgRNA activity by DNA extraction and two rounds of PCR amplification, first to amplify the prdm8 CRIPSR target with gene specific primers containing a M13F extension to the 5’ end of the forward primer (5’TGTAAAACGACGGCCAGT3’) and a second to add a fluorescein tag to the 5’ end of the amplified region (Table 2). .. The fluorescein tagged PCR product was analyzed using capillary gel electrophoresis to detect product length.

    Expressing:

    Article Title: Subcellular drug targeting illuminates local kinase action
    Article Snippet: .. Cellular labeling : SNAP expressing (dox-induced) cells were treated with SNAP-Cell Fluorescein or SNAP-Cell 647-SiR (NEB) for 30 min in serum free DMEM at 37°C and 5% CO2. .. Cells were washed one time and incubated in fresh serum free DMEM for 30 min.

    Article Title: The Giardia cell cycle progresses independently of the anaphase-promoting complex
    Article Snippet: .. Cells expressing SNAP-tagged cyclin B (∼1×108 cells) were iced and harvested, then incubated in 1 ml of complete media containing 5 µM of SNAP-fluorescein (New England Biolabs) for 3 hours at 37°C. .. Cells were chilled on ice for 20 minutes, harvested by centrifugation and resuspended in fresh media containing 10 µM SNAP-Block reagent (New England Biolabs) to start the chase.

    Polymerase Chain Reaction:

    Article Title: Prdm8 regulates pMN progenitor specification for motor neuron and oligodendrocyte fates by modulating Shh signaling response
    Article Snippet: .. The following day injected embryos were assayed for sgRNA activity by DNA extraction and two rounds of PCR amplification, first to amplify the prdm8 CRIPSR target with gene specific primers containing a M13F extension to the 5’ end of the forward primer (5’TGTAAAACGACGGCCAGT3’) and a second to add a fluorescein tag to the 5’ end of the amplified region (Table 2). .. The fluorescein tagged PCR product was analyzed using capillary gel electrophoresis to detect product length.

    Injection:

    Article Title: Prdm8 regulates pMN progenitor specification for motor neuron and oligodendrocyte fates by modulating Shh signaling response
    Article Snippet: .. The following day injected embryos were assayed for sgRNA activity by DNA extraction and two rounds of PCR amplification, first to amplify the prdm8 CRIPSR target with gene specific primers containing a M13F extension to the 5’ end of the forward primer (5’TGTAAAACGACGGCCAGT3’) and a second to add a fluorescein tag to the 5’ end of the amplified region (Table 2). .. The fluorescein tagged PCR product was analyzed using capillary gel electrophoresis to detect product length.

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  • 94
    New England Biolabs snap cell fluorescein
    Mutant septins evade exclusion by quality control if assembled into hetero-octamers before introduction of the wild-type allele. Top, fusion of the <t>SNAP-Tag</t> (SNAP) to the C- terminus of Cdc10(D182N) allows covalent fluorescence pulse labeling of a pool of Cdc10(D182N)-SNAP molecules using <t>BG-DAF.</t> The localization of the labeled mutant Cdc10-SNAP molecules after the introduction of wild-type, untagged Cdc10 via mating was monitored by fluorescence microscopy. Bottom, micrographs taken of budded zygotes generated by mating BG-DAF–labeled cells of strain JTY5169 with CDC10 + cells of strain BY4742. Arrowheads, labeled Cdc10(D182N)-GFP in septin rings at zygote bud necks. Asterisks, labeled Cdc10(D182N)-GFP in septin rings in nonzygote cells. Right, quantification of bud neck fluorescence in budded zygotes, as in Figure 1, B–E .
    Snap Cell Fluorescein, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snap cell fluorescein/product/New England Biolabs
    Average 94 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    snap cell fluorescein - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    Image Search Results


    Mutant septins evade exclusion by quality control if assembled into hetero-octamers before introduction of the wild-type allele. Top, fusion of the SNAP-Tag (SNAP) to the C- terminus of Cdc10(D182N) allows covalent fluorescence pulse labeling of a pool of Cdc10(D182N)-SNAP molecules using BG-DAF. The localization of the labeled mutant Cdc10-SNAP molecules after the introduction of wild-type, untagged Cdc10 via mating was monitored by fluorescence microscopy. Bottom, micrographs taken of budded zygotes generated by mating BG-DAF–labeled cells of strain JTY5169 with CDC10 + cells of strain BY4742. Arrowheads, labeled Cdc10(D182N)-GFP in septin rings at zygote bud necks. Asterisks, labeled Cdc10(D182N)-GFP in septin rings in nonzygote cells. Right, quantification of bud neck fluorescence in budded zygotes, as in Figure 1, B–E .

    Journal: Molecular Biology of the Cell

    Article Title: Cytosolic chaperones mediate quality control of higher-order septin assembly in budding yeast

    doi: 10.1091/mbc.E14-11-1531

    Figure Lengend Snippet: Mutant septins evade exclusion by quality control if assembled into hetero-octamers before introduction of the wild-type allele. Top, fusion of the SNAP-Tag (SNAP) to the C- terminus of Cdc10(D182N) allows covalent fluorescence pulse labeling of a pool of Cdc10(D182N)-SNAP molecules using BG-DAF. The localization of the labeled mutant Cdc10-SNAP molecules after the introduction of wild-type, untagged Cdc10 via mating was monitored by fluorescence microscopy. Bottom, micrographs taken of budded zygotes generated by mating BG-DAF–labeled cells of strain JTY5169 with CDC10 + cells of strain BY4742. Arrowheads, labeled Cdc10(D182N)-GFP in septin rings at zygote bud necks. Asterisks, labeled Cdc10(D182N)-GFP in septin rings in nonzygote cells. Right, quantification of bud neck fluorescence in budded zygotes, as in Figure 1, B–E .

    Article Snippet: A 1 μl amount of 1 mM benzylguanine-diacetylfluorescein (BG-DAF; S9107S, New England Biolabs) dissolved in dimethyl sulfoxide (DMSO) was added, and the culture was rotated in the dark for 30 min.

    Techniques: Mutagenesis, Fluorescence, Labeling, Microscopy, Generated