snap cell fluorescein  (New England Biolabs)


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    Name:
    SNAP Cell Fluorescein
    Description:
    SNAP Cell Fluorescein 50 nmol
    Catalog Number:
    s9107s
    Price:
    307
    Size:
    50 nmol
    Category:
    Fluorochromes
    Buy from Supplier


    Structured Review

    New England Biolabs snap cell fluorescein
    SNAP Cell Fluorescein
    SNAP Cell Fluorescein 50 nmol
    https://www.bioz.com/result/snap cell fluorescein/product/New England Biolabs
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    snap cell fluorescein - by Bioz Stars, 2020-01
    85/100 stars

    Images

    1) Product Images from "Cytosolic chaperones mediate quality control of higher-order septin assembly in budding yeast"

    Article Title: Cytosolic chaperones mediate quality control of higher-order septin assembly in budding yeast

    Journal: Molecular Biology of the Cell

    doi: 10.1091/mbc.E14-11-1531

    Mutant septins evade exclusion by quality control if assembled into hetero-octamers before introduction of the wild-type allele. Top, fusion of the SNAP-Tag (SNAP) to the C- terminus of Cdc10(D182N) allows covalent fluorescence pulse labeling of a pool of Cdc10(D182N)-SNAP molecules using BG-DAF. The localization of the labeled mutant Cdc10-SNAP molecules after the introduction of wild-type, untagged Cdc10 via mating was monitored by fluorescence microscopy. Bottom, micrographs taken of budded zygotes generated by mating BG-DAF–labeled cells of strain JTY5169 with CDC10 + cells of strain BY4742. Arrowheads, labeled Cdc10(D182N)-GFP in septin rings at zygote bud necks. Asterisks, labeled Cdc10(D182N)-GFP in septin rings in nonzygote cells. Right, quantification of bud neck fluorescence in budded zygotes, as in Figure 1, B–E .
    Figure Legend Snippet: Mutant septins evade exclusion by quality control if assembled into hetero-octamers before introduction of the wild-type allele. Top, fusion of the SNAP-Tag (SNAP) to the C- terminus of Cdc10(D182N) allows covalent fluorescence pulse labeling of a pool of Cdc10(D182N)-SNAP molecules using BG-DAF. The localization of the labeled mutant Cdc10-SNAP molecules after the introduction of wild-type, untagged Cdc10 via mating was monitored by fluorescence microscopy. Bottom, micrographs taken of budded zygotes generated by mating BG-DAF–labeled cells of strain JTY5169 with CDC10 + cells of strain BY4742. Arrowheads, labeled Cdc10(D182N)-GFP in septin rings at zygote bud necks. Asterisks, labeled Cdc10(D182N)-GFP in septin rings in nonzygote cells. Right, quantification of bud neck fluorescence in budded zygotes, as in Figure 1, B–E .

    Techniques Used: Mutagenesis, Fluorescence, Labeling, Microscopy, Generated

    Related Articles

    Microscopy:

    Article Title: Analysis of Global Sumoylation Changes Occurring during Keratinocyte Differentiation
    Article Snippet: Paragraph title: Microscopy and imaging ... Fluorescent images were captured after cells were labeled with SNAP-Cell Fluorescein (NEB) per the manufacturer's instructions.

    Centrifugation:

    Article Title: Kinetic pathway of 40S ribosomal subunit recruitment to hepatitis C virus internal ribosome entry site
    Article Snippet: For live cell fluorescent labeling, cells were incubated for 30 min at 37 °C in IMDM containing 5 μM SNAP-fluorescein (S9107S; New England Biolabs). .. Following removal of debris by centrifugation, lysates were incubated with 5 μM SNAP-649 dye (S9159S; New England Biolabs) at 37 °C for 30 min. Sucrose gradient analysis and sample processing followed the method described by Fuchs et al. ( ).

    Article Title: The Giardia cell cycle progresses independently of the anaphase-promoting complex
    Article Snippet: Cells expressing SNAP-tagged cyclin B (∼1×108 cells) were iced and harvested, then incubated in 1 ml of complete media containing 5 µM of SNAP-fluorescein (New England Biolabs) for 3 hours at 37°C. .. Cells were chilled on ice for 20 minutes, harvested by centrifugation and resuspended in fresh media containing 10 µM SNAP-Block reagent (New England Biolabs) to start the chase.

    Mass Spectrometry:

    Article Title: Subcellular drug targeting illuminates local kinase action
    Article Snippet: Ratios of unlabeled to labeled protein were determined using Native MS (Thermo Scientific LTQ Orbitrap XL/Bruker Esquire LC-Ion Trap). .. Cellular labeling : SNAP expressing (dox-induced) cells were treated with SNAP-Cell Fluorescein or SNAP-Cell 647-SiR (NEB) for 30 min in serum free DMEM at 37°C and 5% CO2.

    Article Title: Analysis of Global Sumoylation Changes Occurring during Keratinocyte Differentiation
    Article Snippet: For phase microscopy the cells were imaged using a DP71 camera with an Olympus IX81 microscope using the DP71 controller software; images of the cells were captured after 8 ms exposure using 200× magnification. .. Fluorescent images were captured after cells were labeled with SNAP-Cell Fluorescein (NEB) per the manufacturer's instructions.

    Pulse Chase:

    Article Title: Subcellular drug targeting illuminates local kinase action
    Article Snippet: Cellular labeling : SNAP expressing (dox-induced) cells were treated with SNAP-Cell Fluorescein or SNAP-Cell 647-SiR (NEB) for 30 min in serum free DMEM at 37°C and 5% CO2. .. For pulse-chase labeling experiments, SNAP expressing (dox-induced) cells were treated with DMSO or increasing doses of CLP-BI2536 or CLP-MLN8237 for 1, 2, or 4 hr in serum free DMEM at 37°C and 5% CO2.

    Article Title: The Giardia cell cycle progresses independently of the anaphase-promoting complex
    Article Snippet: Paragraph title: Pulse chase degradation assays ... Cells expressing SNAP-tagged cyclin B (∼1×108 cells) were iced and harvested, then incubated in 1 ml of complete media containing 5 µM of SNAP-fluorescein (New England Biolabs) for 3 hours at 37°C.

    In Vitro:

    Article Title: Subcellular drug targeting illuminates local kinase action
    Article Snippet: Paragraph title: In vitro labeling ... Cellular labeling : SNAP expressing (dox-induced) cells were treated with SNAP-Cell Fluorescein or SNAP-Cell 647-SiR (NEB) for 30 min in serum free DMEM at 37°C and 5% CO2.

    Article Title: Kinetic pathway of 40S ribosomal subunit recruitment to hepatitis C virus internal ribosome entry site
    Article Snippet: For live cell fluorescent labeling, cells were incubated for 30 min at 37 °C in IMDM containing 5 μM SNAP-fluorescein (S9107S; New England Biolabs). .. For in vitro protein labeling, cells were harvested and lysed in lysis buffer.

    Transfection:

    Article Title: The Giardia cell cycle progresses independently of the anaphase-promoting complex
    Article Snippet: The 3HA tag in pcCycB3HAPAC ( ) was replaced with the SNAP tag from pSNAPf (New England Biolabs) and the resulting vector, verified, linearized with NruI and transfected into Giardia cells as described. .. Cells expressing SNAP-tagged cyclin B (∼1×108 cells) were iced and harvested, then incubated in 1 ml of complete media containing 5 µM of SNAP-fluorescein (New England Biolabs) for 3 hours at 37°C.

    Labeling:

    Article Title: Subcellular drug targeting illuminates local kinase action
    Article Snippet: .. Cellular labeling : SNAP expressing (dox-induced) cells were treated with SNAP-Cell Fluorescein or SNAP-Cell 647-SiR (NEB) for 30 min in serum free DMEM at 37°C and 5% CO2. .. Cells were washed one time and incubated in fresh serum free DMEM for 30 min.

    Article Title: Kinetic pathway of 40S ribosomal subunit recruitment to hepatitis C virus internal ribosome entry site
    Article Snippet: .. For live cell fluorescent labeling, cells were incubated for 30 min at 37 °C in IMDM containing 5 μM SNAP-fluorescein (S9107S; New England Biolabs). ..

    Article Title: Analysis of Global Sumoylation Changes Occurring during Keratinocyte Differentiation
    Article Snippet: .. Fluorescent images were captured after cells were labeled with SNAP-Cell Fluorescein (NEB) per the manufacturer's instructions. .. The cells were exposed for 20 ms and images were captured using the Olympus IX81 microscope with the DP71 camera and FITC filter set at 20× magnification.

    Article Title: Cytosolic chaperones mediate quality control of higher-order septin assembly in budding yeast
    Article Snippet: Paragraph title: SNAP-tag in vivo labeling ... A 1 μl amount of 1 mM benzylguanine-diacetylfluorescein (BG-DAF; S9107S, New England Biolabs) dissolved in dimethyl sulfoxide (DMSO) was added, and the culture was rotated in the dark for 30 min.

    Purification:

    Article Title: Subcellular drug targeting illuminates local kinase action
    Article Snippet: The reactions were purified using Zeba columns (Thermo Fisher) and exchanged into a MS compatible buffer (50 mM NH4HCO3, 0.2% HCO2 H). .. Cellular labeling : SNAP expressing (dox-induced) cells were treated with SNAP-Cell Fluorescein or SNAP-Cell 647-SiR (NEB) for 30 min in serum free DMEM at 37°C and 5% CO2.

    In Vivo:

    Article Title: Cytosolic chaperones mediate quality control of higher-order septin assembly in budding yeast
    Article Snippet: Paragraph title: SNAP-tag in vivo labeling ... A 1 μl amount of 1 mM benzylguanine-diacetylfluorescein (BG-DAF; S9107S, New England Biolabs) dissolved in dimethyl sulfoxide (DMSO) was added, and the culture was rotated in the dark for 30 min.

    Concentration Assay:

    Article Title: Subcellular drug targeting illuminates local kinase action
    Article Snippet: In vitro labeling 50 μM SNAP-tag was incubated with 75 μM CLP-linker-inhibitors (or DMSO alone for control reactions) [2.5% (v/v) final DMSO concentration] in buffer [20 mM Tris-Cl (pH 8), 200 mM NaCl, 1 mM DTT (added fresh)] at 26°C for 1.5 hr. .. Cellular labeling : SNAP expressing (dox-induced) cells were treated with SNAP-Cell Fluorescein or SNAP-Cell 647-SiR (NEB) for 30 min in serum free DMEM at 37°C and 5% CO2.

    Incubation:

    Article Title: Subcellular drug targeting illuminates local kinase action
    Article Snippet: In vitro labeling 50 μM SNAP-tag was incubated with 75 μM CLP-linker-inhibitors (or DMSO alone for control reactions) [2.5% (v/v) final DMSO concentration] in buffer [20 mM Tris-Cl (pH 8), 200 mM NaCl, 1 mM DTT (added fresh)] at 26°C for 1.5 hr. .. Cellular labeling : SNAP expressing (dox-induced) cells were treated with SNAP-Cell Fluorescein or SNAP-Cell 647-SiR (NEB) for 30 min in serum free DMEM at 37°C and 5% CO2.

    Article Title: Kinetic pathway of 40S ribosomal subunit recruitment to hepatitis C virus internal ribosome entry site
    Article Snippet: .. For live cell fluorescent labeling, cells were incubated for 30 min at 37 °C in IMDM containing 5 μM SNAP-fluorescein (S9107S; New England Biolabs). ..

    Article Title: The Giardia cell cycle progresses independently of the anaphase-promoting complex
    Article Snippet: .. Cells expressing SNAP-tagged cyclin B (∼1×108 cells) were iced and harvested, then incubated in 1 ml of complete media containing 5 µM of SNAP-fluorescein (New England Biolabs) for 3 hours at 37°C. .. Cells were chilled on ice for 20 minutes, harvested by centrifugation and resuspended in fresh media containing 10 µM SNAP-Block reagent (New England Biolabs) to start the chase.

    other:

    Article Title: Exploiting Ligand-Protein Conjugates to Monitor Ligand-Receptor Interactions
    Article Snippet: SNAP-Cell Fluorescein (BG-fluorescein), SNAP-Cell TMR-Star (BG-tetramethylrhodamine), SNAP-Surface 647 (BG-647) were provided by NEB.

    Imaging:

    Article Title: Analysis of Global Sumoylation Changes Occurring during Keratinocyte Differentiation
    Article Snippet: Paragraph title: Microscopy and imaging ... Fluorescent images were captured after cells were labeled with SNAP-Cell Fluorescein (NEB) per the manufacturer's instructions.

    Expressing:

    Article Title: Subcellular drug targeting illuminates local kinase action
    Article Snippet: .. Cellular labeling : SNAP expressing (dox-induced) cells were treated with SNAP-Cell Fluorescein or SNAP-Cell 647-SiR (NEB) for 30 min in serum free DMEM at 37°C and 5% CO2. .. Cells were washed one time and incubated in fresh serum free DMEM for 30 min.

    Article Title: Kinetic pathway of 40S ribosomal subunit recruitment to hepatitis C virus internal ribosome entry site
    Article Snippet: Protein expression levels in WT and add-back cell lines were quantified by measuring the integrated density on the exposed film. .. For live cell fluorescent labeling, cells were incubated for 30 min at 37 °C in IMDM containing 5 μM SNAP-fluorescein (S9107S; New England Biolabs).

    Article Title: The Giardia cell cycle progresses independently of the anaphase-promoting complex
    Article Snippet: .. Cells expressing SNAP-tagged cyclin B (∼1×108 cells) were iced and harvested, then incubated in 1 ml of complete media containing 5 µM of SNAP-fluorescein (New England Biolabs) for 3 hours at 37°C. .. Cells were chilled on ice for 20 minutes, harvested by centrifugation and resuspended in fresh media containing 10 µM SNAP-Block reagent (New England Biolabs) to start the chase.

    Western Blot:

    Article Title: Kinetic pathway of 40S ribosomal subunit recruitment to hepatitis C virus internal ribosome entry site
    Article Snippet: Proteins were detected by chemiluminescence using Pierce ECL Western Blotting Substrate (Thermo Scientific). .. For live cell fluorescent labeling, cells were incubated for 30 min at 37 °C in IMDM containing 5 μM SNAP-fluorescein (S9107S; New England Biolabs).

    Lysis:

    Article Title: Kinetic pathway of 40S ribosomal subunit recruitment to hepatitis C virus internal ribosome entry site
    Article Snippet: For live cell fluorescent labeling, cells were incubated for 30 min at 37 °C in IMDM containing 5 μM SNAP-fluorescein (S9107S; New England Biolabs). .. For in vitro protein labeling, cells were harvested and lysed in lysis buffer.

    Plasmid Preparation:

    Article Title: The Giardia cell cycle progresses independently of the anaphase-promoting complex
    Article Snippet: The 3HA tag in pcCycB3HAPAC ( ) was replaced with the SNAP tag from pSNAPf (New England Biolabs) and the resulting vector, verified, linearized with NruI and transfected into Giardia cells as described. .. Cells expressing SNAP-tagged cyclin B (∼1×108 cells) were iced and harvested, then incubated in 1 ml of complete media containing 5 µM of SNAP-fluorescein (New England Biolabs) for 3 hours at 37°C.

    Software:

    Article Title: Analysis of Global Sumoylation Changes Occurring during Keratinocyte Differentiation
    Article Snippet: For phase microscopy the cells were imaged using a DP71 camera with an Olympus IX81 microscope using the DP71 controller software; images of the cells were captured after 8 ms exposure using 200× magnification. .. Fluorescent images were captured after cells were labeled with SNAP-Cell Fluorescein (NEB) per the manufacturer's instructions.

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  • 85
    New England Biolabs snap cell fluorescein
    Mutant septins evade exclusion by quality control if assembled into hetero-octamers before introduction of the wild-type allele. Top, fusion of the <t>SNAP-Tag</t> (SNAP) to the C- terminus of Cdc10(D182N) allows covalent fluorescence pulse labeling of a pool of Cdc10(D182N)-SNAP molecules using <t>BG-DAF.</t> The localization of the labeled mutant Cdc10-SNAP molecules after the introduction of wild-type, untagged Cdc10 via mating was monitored by fluorescence microscopy. Bottom, micrographs taken of budded zygotes generated by mating BG-DAF–labeled cells of strain JTY5169 with CDC10 + cells of strain BY4742. Arrowheads, labeled Cdc10(D182N)-GFP in septin rings at zygote bud necks. Asterisks, labeled Cdc10(D182N)-GFP in septin rings in nonzygote cells. Right, quantification of bud neck fluorescence in budded zygotes, as in Figure 1, B–E .
    Snap Cell Fluorescein, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snap cell fluorescein/product/New England Biolabs
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    snap cell fluorescein - by Bioz Stars, 2020-01
    85/100 stars
      Buy from Supplier

    Image Search Results


    Mutant septins evade exclusion by quality control if assembled into hetero-octamers before introduction of the wild-type allele. Top, fusion of the SNAP-Tag (SNAP) to the C- terminus of Cdc10(D182N) allows covalent fluorescence pulse labeling of a pool of Cdc10(D182N)-SNAP molecules using BG-DAF. The localization of the labeled mutant Cdc10-SNAP molecules after the introduction of wild-type, untagged Cdc10 via mating was monitored by fluorescence microscopy. Bottom, micrographs taken of budded zygotes generated by mating BG-DAF–labeled cells of strain JTY5169 with CDC10 + cells of strain BY4742. Arrowheads, labeled Cdc10(D182N)-GFP in septin rings at zygote bud necks. Asterisks, labeled Cdc10(D182N)-GFP in septin rings in nonzygote cells. Right, quantification of bud neck fluorescence in budded zygotes, as in Figure 1, B–E .

    Journal: Molecular Biology of the Cell

    Article Title: Cytosolic chaperones mediate quality control of higher-order septin assembly in budding yeast

    doi: 10.1091/mbc.E14-11-1531

    Figure Lengend Snippet: Mutant septins evade exclusion by quality control if assembled into hetero-octamers before introduction of the wild-type allele. Top, fusion of the SNAP-Tag (SNAP) to the C- terminus of Cdc10(D182N) allows covalent fluorescence pulse labeling of a pool of Cdc10(D182N)-SNAP molecules using BG-DAF. The localization of the labeled mutant Cdc10-SNAP molecules after the introduction of wild-type, untagged Cdc10 via mating was monitored by fluorescence microscopy. Bottom, micrographs taken of budded zygotes generated by mating BG-DAF–labeled cells of strain JTY5169 with CDC10 + cells of strain BY4742. Arrowheads, labeled Cdc10(D182N)-GFP in septin rings at zygote bud necks. Asterisks, labeled Cdc10(D182N)-GFP in septin rings in nonzygote cells. Right, quantification of bud neck fluorescence in budded zygotes, as in Figure 1, B–E .

    Article Snippet: A 1 μl amount of 1 mM benzylguanine-diacetylfluorescein (BG-DAF; S9107S, New England Biolabs) dissolved in dimethyl sulfoxide (DMSO) was added, and the culture was rotated in the dark for 30 min.

    Techniques: Mutagenesis, Fluorescence, Labeling, Microscopy, Generated