autolysosome (New England Biolabs)


Structured Review

Autolysosome, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/autolysosome/product/New England Biolabs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Chemical-induced degradation of PreS2 mutant surface antigen reverses HBV-mediated hepatocarcinogenesis"
Article Title: Chemical-induced degradation of PreS2 mutant surface antigen reverses HBV-mediated hepatocarcinogenesis
Journal: bioRxiv
doi: 10.1101/2022.01.24.477454

Figure Legend Snippet: ABT199 induces PreS2-LHBS degradation through microautophagy (A) NeHep-S2 cells were treated with ABT199 (1uM) or solvent control (DMSO), and the mRNA level was measured by qPCR. The bar plot shows the mRNA level relative to ACTB mRNA and normalized to solvent control (DMSO) treated cells. (B) Examined the activation of macroautophagy. Upper panel: the intensity profile of NeHep-S2 cells staining for PreS2 mutant LHBS and autolysosome. Bottom panel: after incubated with solvent control (DMSO), ABT199 (1uM), or in serum-free medium for 6hr, ten thousand stained cells were analyzed by flow cytometry. The density plot shows the cell counts for the intensity of PreS2-LHBS and autolysosome in each condition. (C) Investigation of ABT199-induced pathway for PreS2 mutant LHBS degradation. Nehep-s2 cells were treated with indicated inhibitors with or without ABT199 (1uM), and the protein expression was analyzed by immunoblotting. The level of CyclinB1 and LC3B served as a positive control for inhibitor treatment. Upper panel: representative blot. Bottom panel: bar plot shows the expression normalized to solvent control (DMSO) treated cells and loading control (GAPDH). (D) NeHep-S2 cells were treated with ABT199 (1uM) for 6hr, and the colocalization of PreS2 mutant LHBS, Lamp1, and Rab7 were investigated by immunofluorescent staining. Scale bar donates 20um. (E) Depletion of NPC1 rescued ABT199-induced PreS2 mutant LHBS degradation. NeHep-S2 were knockdown by transfection with a control siRNA or NPC1-specific siRNA, and the protein expression was examined after 48hr post-transfection by immunoblotting. Left panel: representative blot. Right panel: bar plot shows the expression normalized to loading control (GAPDH) and cells transfected with control siRNA. Error bar shows SEM. **P
Techniques Used: Real-time Polymerase Chain Reaction, Activation Assay, Staining, Mutagenesis, Incubation, Flow Cytometry, Expressing, Positive Control, Transfection