snap cell 505 star (New England Biolabs)
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Name:
SNAP Cell 505 Star
Description:
SNAP Cell 505 Star 50 nmol
Catalog Number:
S9103S
Price:
344
Category:
Fluorochromes
Size:
50 nmol
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Structured Review
New England Biolabs
snap cell 505 star
SNAP Cell 505 Star 50 nmol
https://www.bioz.com/result/snap cell 505 star/product/New England Biolabs
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
SNAP Cell 505 Star 50 nmol
https://www.bioz.com/result/snap cell 505 star/product/New England Biolabs
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
snap cell 505 star - by Bioz Stars,
2021-04
92/100 stars
Images
1) Product Images from "Engineering a living biomaterial via bacterial surface capture of environmental molecules"
Article Title: Engineering a living biomaterial via bacterial surface capture of environmental molecules
Journal: Synthetic Biology
doi: 10.1093/synbio/ysy017
Figure Legend Snippet: Examination of the functionality of SNAP was determined by using a fluorescent label modified with a BG group (SNAP-Cell ® 505-Star). ( a ) Schematic of surface-display system and ( b ) fluorescent label addition. ( c ) Histogram and ( d ) bar graph from flow cytometry analysis showing the shift in fluorescence of cells labelled after growth with and without inducer. Tests were performed in triplicate with 10 000 events per sample (* P
Techniques Used: Modification, Flow Cytometry, Fluorescence
2) Product Images from "Engineering a living biomaterial via bacterial surface capture of environmental molecules"
Article Title: Engineering a living biomaterial via bacterial surface capture of environmental molecules
Journal: Synthetic Biology
doi: 10.1093/synbio/ysy017
Figure Legend Snippet: Examination of the functionality of SNAP was determined by using a fluorescent label modified with a BG group (SNAP-Cell ® 505-Star). ( a ) Schematic of surface-display system and ( b ) fluorescent label addition. ( c ) Histogram and ( d ) bar graph from flow cytometry analysis showing the shift in fluorescence of cells labelled after growth with and without inducer. Tests were performed in triplicate with 10 000 events per sample (* P
Techniques Used: Modification, Flow Cytometry, Fluorescence
3) Product Images from "Differential HDAC1/2 network analysis reveals a role for prefoldin/CCT in HDAC1/2 complex assembly"
Article Title: Differential HDAC1/2 network analysis reveals a role for prefoldin/CCT in HDAC1/2 complex assembly
Journal: Scientific Reports
doi: 10.1038/s41598-018-32009-w
Figure Legend Snippet: HDAC1 associates with components of the prefoldin-mediated CCT folding pathway. ( A used to model possible HDAC1 processing by the prefoldin/CCT pathway in the nucleus. ( B . ( C ) VBP1 colocalises with HDAC1 in the nucleus. Flp-In™-293 host cells stably expressing Halo-HDAC1 were transiently transfected with SNAP-FLAG-VBP1 and proteins imaged using HaloTag® TMRDirect™ ligand (Halo-HDAC1; red), SNAP-Cell® 505-Star ligand (SNAP-FLAG-VBP1; green), and Hoechst dye (nuclei; blue). ( D network showing established TCP1 (CCT complex) interacting proteins was generated using the following settings: minimum required interaction score - medium confidence (0.4); maximum number of interactors - 50; active interaction source – Experiments; clustering method – kmeans (default settings). Clusters are indicated by node color.
Techniques Used: Stable Transfection, Expressing, Transfection, Generated
4) Product Images from "Engineering a living biomaterial via bacterial surface capture of environmental molecules"
Article Title: Engineering a living biomaterial via bacterial surface capture of environmental molecules
Journal: Synthetic Biology
doi: 10.1093/synbio/ysy017
Figure Legend Snippet: Examination of the functionality of SNAP was determined by using a fluorescent label modified with a BG group (SNAP-Cell ® 505-Star). ( a ) Schematic of surface-display system and ( b ) fluorescent label addition. ( c ) Histogram and ( d ) bar graph from flow cytometry analysis showing the shift in fluorescence of cells labelled after growth with and without inducer. Tests were performed in triplicate with 10 000 events per sample (* P
Techniques Used: Modification, Flow Cytometry, Fluorescence
5) Product Images from "Differential HDAC1/2 network analysis reveals a role for prefoldin/CCT in HDAC1/2 complex assembly"
Article Title: Differential HDAC1/2 network analysis reveals a role for prefoldin/CCT in HDAC1/2 complex assembly
Journal: Scientific Reports
doi: 10.1038/s41598-018-32009-w
Figure Legend Snippet: HDAC1 associates with components of the prefoldin-mediated CCT folding pathway. ( A ) The established mechanism for prefoldin delivery of client proteins to the CCT complex 37 , 44 , 65 used to model possible HDAC1 processing by the prefoldin/CCT pathway in the nucleus. ( B ) Prefoldin subunit VBP1 interacts with HDAC1. HEK293T cell lysates expressing tagged VBP1 with or without Halo-tagged versions of HDAC1 were used for Halo affinity purification. Samples were analysed by SDS PAGE followed by Western blotting or Coomassie staining. SNAP-FLAG-VBP1 was detected using anti-FLAG mouse monoclonal primary antibody and IRDye® 680LT labeled goat anti-Mouse secondary antibody. Halo-tagged HDAC1 was detected using anti-HDAC1 rabbit polyclonal primary antibody and IRDye® 800CW labeled goat anti-Rabbit secondary antibody. Full length images are presented in Supplementary Figure 3 . ( C ) VBP1 colocalises with HDAC1 in the nucleus. Flp-In™-293 host cells stably expressing Halo-HDAC1 were transiently transfected with SNAP-FLAG-VBP1 and proteins imaged using HaloTag® TMRDirect™ ligand (Halo-HDAC1; red), SNAP-Cell® 505-Star ligand (SNAP-FLAG-VBP1; green), and Hoechst dye (nuclei; blue). ( D ) TCP1 and other CCT complex components interact with HDAC1. The STRING 38 network showing established TCP1 (CCT complex) interacting proteins was generated using the following settings: minimum required interaction score - medium confidence (0.4); maximum number of interactors - 50; active interaction source – Experiments; clustering method – kmeans (default settings). Clusters are indicated by node color.
Techniques Used: Expressing, Affinity Purification, SDS Page, Western Blot, Staining, Labeling, Stable Transfection, Transfection, Generated
6) Product Images from "BRK phosphorylates SMAD4 for proteasomal degradation and inhibits tumor suppressor FRK to control SNAIL, SLUG, and metastatic potential"
Article Title: BRK phosphorylates SMAD4 for proteasomal degradation and inhibits tumor suppressor FRK to control SNAIL, SLUG, and metastatic potential
Journal: Science Advances
doi: 10.1126/sciadv.aaw3113
![Ectopically expressed BRK and SMAD4 interact and colocalize in HEK293 cells. ( A and B ) SNAP-FLAG-BRK-YF (SF-BRK-YF) and HALO-SMAD2/3/4 were expressed into HEK293 cells, and cell lysates were subjected to affinity purification (AP) with Halo magnetic beads (A) or SNAP capture magnetic beads (B) antibodies, followed by immunoblotting using anti-Halo and anti-FLAG antibodies. Bottom: The ectopic expression of BRK and SMAD2/SMAD3/SMAD4 as detected by anti-Halo and anti-FLAG antibodies. During affinity purification, either the SNAP_Flag or Halo tags were clipped off using Precision or Tev proteases, respectively. Halo-SMAD4 is ~93 kDa; after Halo-Tag removal, SMAD4 is ~60 kDa. Similarly, SNAP-Flag-BRK is ~73 kDa, and after SNAP-Tag removal, BRK is ~50 kDa. In (B), the three different blots in the middle were probed with the SMAD2, SMAD3, and SMAD4 antibodies. ( C ) HEK293 cells were cotransfected with SF-BRK-YF, Halo-SMAD2, Halo-SMAD3, and Halo-SMAD4, and the cell lysates were subjected to affinity purification with Halo magnetic beads or SNAP capture magnetic beads, followed by immunoblotting using anti-SMAD2, anti-SMAD3, anti-SMAD4, and anti-Flag antibodies. Total cell lysates were also analyzed by immunoblotting using antibodies against Halo-SMAD2, Halo-SMAD3, Halo-SMAD4, and Flag. ( D ) GFP-SMAD4 was cotransfected with BRK-W44A, BRK-∆SH2, BRK-∆SH3, BRK-WT, BRK-Y342F, or BRK-YF. The corresponding protein extracts were immunoprecipitated with anti-GFP and mouse immunoglobulin G (IgG; lysates from SMAD4 and BRK-YF cotransfected cells as a representative) and immunoblotted with anti-BRK and anti-GFP antibodies and β-actin as a loading control. IP, immunoprecipitation. ( E ) SF-BRK-YF was expressed either alone or with Halo-SMAD4 [full length (FL)] or SMAD4 deletion mutants (A to E) in HEK293 cells. Total cell lysates were subjected to Halo affinity purification and analyzed by immunoblotting with FLAG and Halo antibodies. ( F ) Halo-SMAD4 or Halo plasmid alone with SF-BRK-WT or SF-BRK-YF was ectopically expressed in HEK293T cells. SNAP affinity purification followed by MudPIT mass spectrometry analysis showed that Halo-SMAD4 copurified with SNAP-Flag-BRK-WT or SNAP-Flag-BRK-YF but not with Halo alone. ( G ) Halo-SMAD4 or SF-BRK-YF alone or in combination was transfected into HEK293T cells. Halo-Tag TMRDirect fluorescent ligand (red) and SNAP-Cell 505-Star (green) were used to label Halo-Tag and SNAP-Tag proteins, respectively; DNA was stained with Hoechst dye (blue). DAPI, 4′,6-diamidino-2-phenylindole.](https://storage.googleapis.com/bioz_article_images/PMC6810434/aaw3113-F2.jpg "... in HEK293 cells. ( A and B ) SNAP-FLAG-BRK-YF (SF-BRK-YF) and HALO-SMAD2/3/4 were expressed into HEK293 cells, ...")
Figure Legend Snippet: Ectopically expressed BRK and SMAD4 interact and colocalize in HEK293 cells. ( A and B ) SNAP-FLAG-BRK-YF (SF-BRK-YF) and HALO-SMAD2/3/4 were expressed into HEK293 cells, and cell lysates were subjected to affinity purification (AP) with Halo magnetic beads (A) or SNAP capture magnetic beads (B) antibodies, followed by immunoblotting using anti-Halo and anti-FLAG antibodies. Bottom: The ectopic expression of BRK and SMAD2/SMAD3/SMAD4 as detected by anti-Halo and anti-FLAG antibodies. During affinity purification, either the SNAP_Flag or Halo tags were clipped off using Precision or Tev proteases, respectively. Halo-SMAD4 is ~93 kDa; after Halo-Tag removal, SMAD4 is ~60 kDa. Similarly, SNAP-Flag-BRK is ~73 kDa, and after SNAP-Tag removal, BRK is ~50 kDa. In (B), the three different blots in the middle were probed with the SMAD2, SMAD3, and SMAD4 antibodies. ( C ) HEK293 cells were cotransfected with SF-BRK-YF, Halo-SMAD2, Halo-SMAD3, and Halo-SMAD4, and the cell lysates were subjected to affinity purification with Halo magnetic beads or SNAP capture magnetic beads, followed by immunoblotting using anti-SMAD2, anti-SMAD3, anti-SMAD4, and anti-Flag antibodies. Total cell lysates were also analyzed by immunoblotting using antibodies against Halo-SMAD2, Halo-SMAD3, Halo-SMAD4, and Flag. ( D ) GFP-SMAD4 was cotransfected with BRK-W44A, BRK-∆SH2, BRK-∆SH3, BRK-WT, BRK-Y342F, or BRK-YF. The corresponding protein extracts were immunoprecipitated with anti-GFP and mouse immunoglobulin G (IgG; lysates from SMAD4 and BRK-YF cotransfected cells as a representative) and immunoblotted with anti-BRK and anti-GFP antibodies and β-actin as a loading control. IP, immunoprecipitation. ( E ) SF-BRK-YF was expressed either alone or with Halo-SMAD4 [full length (FL)] or SMAD4 deletion mutants (A to E) in HEK293 cells. Total cell lysates were subjected to Halo affinity purification and analyzed by immunoblotting with FLAG and Halo antibodies. ( F ) Halo-SMAD4 or Halo plasmid alone with SF-BRK-WT or SF-BRK-YF was ectopically expressed in HEK293T cells. SNAP affinity purification followed by MudPIT mass spectrometry analysis showed that Halo-SMAD4 copurified with SNAP-Flag-BRK-WT or SNAP-Flag-BRK-YF but not with Halo alone. ( G ) Halo-SMAD4 or SF-BRK-YF alone or in combination was transfected into HEK293T cells. Halo-Tag TMRDirect fluorescent ligand (red) and SNAP-Cell 505-Star (green) were used to label Halo-Tag and SNAP-Tag proteins, respectively; DNA was stained with Hoechst dye (blue). DAPI, 4′,6-diamidino-2-phenylindole.
Techniques Used: Affinity Purification, Magnetic Beads, Expressing, Immunoprecipitation, Plasmid Preparation, Mass Spectrometry, Transfection, Staining
Related Articles
Binding Assay:Article Title: Engineering a living biomaterial via bacterial surface capture of environmental molecules Article Snippet: 2.4 Fluorescence labelling SNAP-Cell® 505-Star is a fluorescent label purchased from New England Biolabs, Inc., with an excitation peak at 504 nm and an emission peak at 532 nm. .. Labeling:Article Title: Engineering a living biomaterial via bacterial surface capture of environmental molecules Article Snippet: 2.4 Fluorescence labelling SNAP-Cell® 505-Star is a fluorescent label purchased from New England Biolabs, Inc., with an excitation peak at 504 nm and an emission peak at 532 nm. .. Article Title: Histone Methylation by SETD1A Protects Nascent DNA through the Nucleosome Chaperone Activity of FANCD2 Article Snippet: U-2-OS cells were grown on glass coverslips, transfected with siRNA, incubated for 48 hr, and transfected with pSNAPm-H3.1 using FuGene HD. .. 24 hr post DNA transfection, pre-existing SNAP-H3.1 was labeled with Article Title: BRK phosphorylates SMAD4 for proteasomal degradation and inhibits tumor suppressor FRK to control SNAIL, SLUG, and metastatic potential Article Snippet: Live-cell imaging HEK293T cells were seeded onto glass-bottom culture dishes (MatTek, Ashland, MA) and transiently transfected with the SNAP-Flag-BRK and Halo-SMAD4 constructs. .. Affinity-tagged proteins were fluorescently labeled during growth, either with Halo-Tag TMRDirect ligand (Promega) or Transfection:Article Title: Histone Methylation by SETD1A Protects Nascent DNA through the Nucleosome Chaperone Activity of FANCD2 Article Snippet: U-2-OS cells were grown on glass coverslips, transfected with siRNA, incubated for 48 hr, and transfected with pSNAPm-H3.1 using FuGene HD. .. 24 hr post DNA transfection, pre-existing SNAP-H3.1 was labeled with Fluorescence:Article Title: Engineering a living biomaterial via bacterial surface capture of environmental molecules Article Snippet: A 5 min incubation at 95°C followed by 10 min at −80°C were added in front of the touchdown PCR program for whole-cell PCR. .. 2.4 Incubation:Article Title: Referenced Single-Molecule Measurements Differentiate between GPCR Oligomerization States Article Snippet: A stock solution of HaloTag TMR Ligand (Promega, Madison, WI) was diluted in supplemented DMEM to a final concentration of 5 nM. .. After incubation posttransfection, all medium was removed from the cells and replaced with supplemented DMEM containing 5 nM HaloTag TMR Ligand (Promega) and 5 μ |