vil 6 cbd  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85
    Name:
    Chitin Resin
    Description:
    Chitin Resin 100 ml
    Catalog Number:
    s6651l
    Price:
    437
    Size:
    100 ml
    Category:
    Protein Purification Kits
    Buy from Supplier


    Structured Review

    New England Biolabs vil 6 cbd
    Chitin Resin
    Chitin Resin 100 ml
    https://www.bioz.com/result/vil 6 cbd/product/New England Biolabs
    Average 85 stars, based on 198 article reviews
    Price from $9.99 to $1999.99
    vil 6 cbd - by Bioz Stars, 2020-05
    85/100 stars

    Images

    1) Product Images from "Determinants of Secretion and Intracellular Localization of Human Herpesvirus 8 Interleukin-6 ▿"

    Article Title: Determinants of Secretion and Intracellular Localization of Human Herpesvirus 8 Interleukin-6 ▿

    Journal: Journal of Virology

    doi: 10.1128/JVI.02625-08

    vIL-6-calnexin interactions and vIL-6 conformation. (A) Coprecipitation assays to assess IL-6-chaperone protein interactions, using vIL-6-CBD (v) and hIL-6-KDEL-CBD (hK) and immunoblot detection of chitin-bead coprecipitated calnexin and calreticulin.
    Figure Legend Snippet: vIL-6-calnexin interactions and vIL-6 conformation. (A) Coprecipitation assays to assess IL-6-chaperone protein interactions, using vIL-6-CBD (v) and hIL-6-KDEL-CBD (hK) and immunoblot detection of chitin-bead coprecipitated calnexin and calreticulin.

    Techniques Used:

    2) Product Images from "Determinants of Secretion and Intracellular Localization of Human Herpesvirus 8 Interleukin-6 ▿"

    Article Title: Determinants of Secretion and Intracellular Localization of Human Herpesvirus 8 Interleukin-6 ▿

    Journal: Journal of Virology

    doi: 10.1128/JVI.02625-08

    C-helix residues and vIL-6 secretion.
    Figure Legend Snippet: C-helix residues and vIL-6 secretion.

    Techniques Used:

    Influences of calnexin and gp130 on secretion and expression vIL-6. (A) Interactions of calnexin with vIL-6 domain substitution variants (see Fig. ) by coprecipitation. SP, signal peptide; A-D1/D2, helices A-C and proximal (D1) and distal
    Figure Legend Snippet: Influences of calnexin and gp130 on secretion and expression vIL-6. (A) Interactions of calnexin with vIL-6 domain substitution variants (see Fig. ) by coprecipitation. SP, signal peptide; A-D1/D2, helices A-C and proximal (D1) and distal

    Techniques Used: Expressing

    vIL-6-calnexin interactions and vIL-6 conformation. (A) Coprecipitation assays to assess IL-6-chaperone protein interactions, using vIL-6-CBD (v) and hIL-6-KDEL-CBD (hK) and immunoblot detection of chitin-bead coprecipitated calnexin and calreticulin.
    Figure Legend Snippet: vIL-6-calnexin interactions and vIL-6 conformation. (A) Coprecipitation assays to assess IL-6-chaperone protein interactions, using vIL-6-CBD (v) and hIL-6-KDEL-CBD (hK) and immunoblot detection of chitin-bead coprecipitated calnexin and calreticulin.

    Techniques Used:

    Distinguishing between membrane-free and membrane-inserted vIL-6 in microsomes. (A) Microsomal membrane preparations (see Materials and Methods) from vIL-6 vector-transfected HEK293T cells were either untreated (“input”) or treated with
    Figure Legend Snippet: Distinguishing between membrane-free and membrane-inserted vIL-6 in microsomes. (A) Microsomal membrane preparations (see Materials and Methods) from vIL-6 vector-transfected HEK293T cells were either untreated (“input”) or treated with

    Techniques Used: Plasmid Preparation, Transfection

    Iodixanol gradient membrane fractionation for identification of vIL-6 subcellular distribution in HEK293T (293T) and calnexin (Cnx − )- and calreticulin (Crt − )-depleted cells in the absence or presence of transfected gp130. Chitin bead-precipitated
    Figure Legend Snippet: Iodixanol gradient membrane fractionation for identification of vIL-6 subcellular distribution in HEK293T (293T) and calnexin (Cnx − )- and calreticulin (Crt − )-depleted cells in the absence or presence of transfected gp130. Chitin bead-precipitated

    Techniques Used: Fractionation, Transfection

    Secretion of domain-substitution variants of vIL-6 and hIL-6. (A) Diagrammatic representation of vIL-6 domain substitution variants, involving exchange of N-terminal, α-helical, and A-B loop regions of vIL-6 with equivalent, colinear sequences
    Figure Legend Snippet: Secretion of domain-substitution variants of vIL-6 and hIL-6. (A) Diagrammatic representation of vIL-6 domain substitution variants, involving exchange of N-terminal, α-helical, and A-B loop regions of vIL-6 with equivalent, colinear sequences

    Techniques Used:

    ). Bold lettering represents vIL-6 amino acid residues of the C-helical region (top line); gray lettering corresponds to colinear hIL-6
    Figure Legend Snippet: ). Bold lettering represents vIL-6 amino acid residues of the C-helical region (top line); gray lettering corresponds to colinear hIL-6

    Techniques Used:

    Signaling and gp130-binding properties of vIL-6 C-helix point variants. (A) Functional interaction of vIL-6 proteins with gp130, as determined by Western blotting for phosphorylated (active) STAT3 (pSTAT3) in cell extracts from vIL-6 vector-transfected
    Figure Legend Snippet: Signaling and gp130-binding properties of vIL-6 C-helix point variants. (A) Functional interaction of vIL-6 proteins with gp130, as determined by Western blotting for phosphorylated (active) STAT3 (pSTAT3) in cell extracts from vIL-6 vector-transfected

    Techniques Used: Binding Assay, Functional Assay, Western Blot, Plasmid Preparation, Transfection

    Confocal fluorescence microscopy analysis of vIL-6 distribution in response to gp130. HEK293T (293T) and calnexin-depleted (Cnx − ) cells were cotransfected with expression plasmids for vIL-6-RFP and ER-targeted GFP-KDEL, with or without cotransfected
    Figure Legend Snippet: Confocal fluorescence microscopy analysis of vIL-6 distribution in response to gp130. HEK293T (293T) and calnexin-depleted (Cnx − ) cells were cotransfected with expression plasmids for vIL-6-RFP and ER-targeted GFP-KDEL, with or without cotransfected

    Techniques Used: Fluorescence, Microscopy, Expressing

    Related Articles

    Transfection:

    Article Title: Promotion of Endoplasmic Reticulum-Associated Degradation of Procathepsin D by Human Herpesvirus 8-Encoded Viral Interleukin-6
    Article Snippet: .. Two days posttransfection, lysates from transfected cultures were prepared by suspending centrifugation-derived cell pellets in lysis buffer (50 mM Tris HCl [pH 7.5], 150 mM NaCl, 5 mM EDTA, and 1% digitonin, complemented with protease inhibitor cocktail [Sigma, catalog no. P8340]), and to samples of lysate were added chitin beads (New England BioLabs; catalog no. S6651L), StrepII-binding StrepTactin beads (Qiagen; catalog no. 1057979), or S protein beads (EMD Millipore; catalog no. E69704), as appropriate, and the mixtures were incubated at 4°C overnight, with rocking. .. Chitin- and S-protein-bound precipitates were washed in lysis buffer by repeated suspension and centrifugation prior to 95°C incubation with denaturation buffer (188 mM Tris HCl [pH 6.8], 3% SDS, 30% glycerol, 15% β-mercaptoethanol, and 0.01% bromophenol blue) and loading onto SDS-PAGE gels for size fractionation and Western blotting.

    Protease Inhibitor:

    Article Title: Promotion of Endoplasmic Reticulum-Associated Degradation of Procathepsin D by Human Herpesvirus 8-Encoded Viral Interleukin-6
    Article Snippet: .. Two days posttransfection, lysates from transfected cultures were prepared by suspending centrifugation-derived cell pellets in lysis buffer (50 mM Tris HCl [pH 7.5], 150 mM NaCl, 5 mM EDTA, and 1% digitonin, complemented with protease inhibitor cocktail [Sigma, catalog no. P8340]), and to samples of lysate were added chitin beads (New England BioLabs; catalog no. S6651L), StrepII-binding StrepTactin beads (Qiagen; catalog no. 1057979), or S protein beads (EMD Millipore; catalog no. E69704), as appropriate, and the mixtures were incubated at 4°C overnight, with rocking. .. Chitin- and S-protein-bound precipitates were washed in lysis buffer by repeated suspension and centrifugation prior to 95°C incubation with denaturation buffer (188 mM Tris HCl [pH 6.8], 3% SDS, 30% glycerol, 15% β-mercaptoethanol, and 0.01% bromophenol blue) and loading onto SDS-PAGE gels for size fractionation and Western blotting.

    Centrifugation:

    Article Title: Promotion of Endoplasmic Reticulum-Associated Degradation of Procathepsin D by Human Herpesvirus 8-Encoded Viral Interleukin-6
    Article Snippet: .. Two days posttransfection, lysates from transfected cultures were prepared by suspending centrifugation-derived cell pellets in lysis buffer (50 mM Tris HCl [pH 7.5], 150 mM NaCl, 5 mM EDTA, and 1% digitonin, complemented with protease inhibitor cocktail [Sigma, catalog no. P8340]), and to samples of lysate were added chitin beads (New England BioLabs; catalog no. S6651L), StrepII-binding StrepTactin beads (Qiagen; catalog no. 1057979), or S protein beads (EMD Millipore; catalog no. E69704), as appropriate, and the mixtures were incubated at 4°C overnight, with rocking. .. Chitin- and S-protein-bound precipitates were washed in lysis buffer by repeated suspension and centrifugation prior to 95°C incubation with denaturation buffer (188 mM Tris HCl [pH 6.8], 3% SDS, 30% glycerol, 15% β-mercaptoethanol, and 0.01% bromophenol blue) and loading onto SDS-PAGE gels for size fractionation and Western blotting.

    Purification:

    Article Title: Recombinant expression of twelve evolutionarily diverse subfamily I? aminotransferases
    Article Snippet: .. They were purified over chitin resin (New England Biolabs) in buffer containing 20 mM sodium phosphate, pH 8.0 with 500 mM NaCl and 10 μM PLP. .. The resin-bound protein was incubated overnight in the same buffer with 50 mM DTT to catalyze cleavage of the tag, and eluted with 20 mM potassium phosphate buffer containing 10 μM PLP.

    Article Title: Unique Helicase Determinants in the Essential Conjugative TraI Factor from Salmonella enterica Serovar Typhimurium Plasmid pCU1
    Article Snippet: .. All proteins were purified on chitin resin (New England BioLabs) using a batch bind method followed by an extended wash step as described in the manufacturer's protocol. .. The chitin resin was incubated for 16 h with 50 mM dithiothreitol (DTT) to induce cleavage of the intein and chitin binding domain (CBD) tags and thus release TraI from the intein, CBD, and chitin resin.

    Plasmid Purification:

    Article Title: Recombinant expression of twelve evolutionarily diverse subfamily I? aminotransferases
    Article Snippet: .. They were purified over chitin resin (New England Biolabs) in buffer containing 20 mM sodium phosphate, pH 8.0 with 500 mM NaCl and 10 μM PLP. .. The resin-bound protein was incubated overnight in the same buffer with 50 mM DTT to catalyze cleavage of the tag, and eluted with 20 mM potassium phosphate buffer containing 10 μM PLP.

    Incubation:

    Article Title: Promotion of Endoplasmic Reticulum-Associated Degradation of Procathepsin D by Human Herpesvirus 8-Encoded Viral Interleukin-6
    Article Snippet: .. Two days posttransfection, lysates from transfected cultures were prepared by suspending centrifugation-derived cell pellets in lysis buffer (50 mM Tris HCl [pH 7.5], 150 mM NaCl, 5 mM EDTA, and 1% digitonin, complemented with protease inhibitor cocktail [Sigma, catalog no. P8340]), and to samples of lysate were added chitin beads (New England BioLabs; catalog no. S6651L), StrepII-binding StrepTactin beads (Qiagen; catalog no. 1057979), or S protein beads (EMD Millipore; catalog no. E69704), as appropriate, and the mixtures were incubated at 4°C overnight, with rocking. .. Chitin- and S-protein-bound precipitates were washed in lysis buffer by repeated suspension and centrifugation prior to 95°C incubation with denaturation buffer (188 mM Tris HCl [pH 6.8], 3% SDS, 30% glycerol, 15% β-mercaptoethanol, and 0.01% bromophenol blue) and loading onto SDS-PAGE gels for size fractionation and Western blotting.

    Article Title: Enzyme-catalyzed expressed protein ligation
    Article Snippet: .. After 1 hour of incubation at 4°C, the lysate was drained from the cellulose and then bound to 5 ml chitin resin (NEB). .. The resin was then washed with 150 ml washing buffer (250 mM NaCl, 25 mM HEPES, 0.1% Triton X-100, pH 7.5), incubated overnight in cleavage buffer (250 mM NaCl, 50 mM HEPES, 300 mM MESNA, pH 7.5).

    Lysis:

    Article Title: Promotion of Endoplasmic Reticulum-Associated Degradation of Procathepsin D by Human Herpesvirus 8-Encoded Viral Interleukin-6
    Article Snippet: .. Two days posttransfection, lysates from transfected cultures were prepared by suspending centrifugation-derived cell pellets in lysis buffer (50 mM Tris HCl [pH 7.5], 150 mM NaCl, 5 mM EDTA, and 1% digitonin, complemented with protease inhibitor cocktail [Sigma, catalog no. P8340]), and to samples of lysate were added chitin beads (New England BioLabs; catalog no. S6651L), StrepII-binding StrepTactin beads (Qiagen; catalog no. 1057979), or S protein beads (EMD Millipore; catalog no. E69704), as appropriate, and the mixtures were incubated at 4°C overnight, with rocking. .. Chitin- and S-protein-bound precipitates were washed in lysis buffer by repeated suspension and centrifugation prior to 95°C incubation with denaturation buffer (188 mM Tris HCl [pH 6.8], 3% SDS, 30% glycerol, 15% β-mercaptoethanol, and 0.01% bromophenol blue) and loading onto SDS-PAGE gels for size fractionation and Western blotting.

    Affinity Purification:

    Article Title: The eto1, eto2, and eto3 Mutations and Cytokinin Treatment Increase Ethylene Biosynthesis in Arabidopsis by Increasing the Stability of ACS Protein
    Article Snippet: .. The soluble ACS5 fusion protein was affinity purified using chitin resin as described by the manufacturer (New England Biolabs), and the ACS5 proteins were released from the chitin column by intein-mediated self-cleavage in the presence of 50 mM DTT, yielding ACS5 protein lacking any fused domain. .. The protein fractions from each purification step were analyzed by SDS-PAGE to monitor the purification.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    New England Biolabs chitin resin
    Chitin Resin, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chitin resin/product/New England Biolabs
    Average 98 stars, based on 65 article reviews
    Price from $9.99 to $1999.99
    chitin resin - by Bioz Stars, 2020-05
    98/100 stars
      Buy from Supplier

    Image Search Results