polya spin mrna isolation kit  (New England Biolabs)


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    Name:
    polyA Spin mRNA Isolation Kit
    Description:
    polyA Spin mRNA Isolation Kit 8 isolations
    Catalog Number:
    s1560s
    Price:
    228
    Size:
    8 isolations
    Category:
    mRNA Purification Kits
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    New England Biolabs polya spin mrna isolation kit
    polyA Spin mRNA Isolation Kit
    polyA Spin mRNA Isolation Kit 8 isolations
    https://www.bioz.com/result/polya spin mrna isolation kit/product/New England Biolabs
    Average 97 stars, based on 734 article reviews
    Price from $9.99 to $1999.99
    polya spin mrna isolation kit - by Bioz Stars, 2020-08
    97/100 stars

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    1) Product Images from "piRNA-like small RNAs mark extended 3’UTRs present in germ and somatic cells"

    Article Title: piRNA-like small RNAs mark extended 3’UTRs present in germ and somatic cells

    Journal: BMC Genomics

    doi: 10.1186/s12864-015-1662-6

    piRNA clusters mark extended 3’ UTRs. Potential precursors of extended 3’ UTR piRNA clusters are detectable as 3’ UTR isoforms in testis and in somatic tissues. a Representative example of a piRNA cluster that extends beyond an annotated 3’ UTR. The annotated 3’UTR is shown in purple and the piRNA cluster is shown in red. Individual piRNA alignments are in blue (sense strand) and red (antisense strand) at the bottom. Genomic features are visualized using the Integrative Genomics Viewer [ 51 ]. b Unannotated portions of 3’ UTR piRNA clusters are enriched for polyA+ RNA expression when compared to randomly selected unannotated regions. We used an adult testis RNA-Seq dataset [ 18 ] to determine expression of the unannotated portions of the extended 3’ UTR clusters (dark blue) or random intergenic regions of equal size (light blue). This indicates that the unannotated extended 3’UTRs are transcribed in the testis. c Expression of the unannotated portion of extended 3’ UTR clusters (X-axis) compared with expression of the corresponding annotated mRNA (Y-axis) in the adult testis RNA-Seq dataset [ 18 ]. The annotated portions of genes are generally expressed at higher RPKM than the unannotated portion of the 3’UTR. d Schematic of Northern blot probes, RT-PCR primers, and PCR amplicons. e Northern blot of Pdpr with an exonic probe (top) shows bands consistent with the length of the annotated mRNA, plus a band consistent with addition of the unannotated region corresponding to the piRNA cluster. The bands are visible only in brain. Probing of the same blot with the unannotated 3’UTR probe (bottom) shows a single band in the same position as the upper band in the exonic probe blot. f Representative RT-PCR results of the three Pdpr amplicons shown schematically in D and marked to the right, with (+) and without (−) reverse transcriptase during the cDNA synthesis step (top). g Summary of RT-PCR products obtained from 10 transcripts with extended 3’ UTR piRNA clusters. For each gene testis, brain, spleen, and liver were tested. All transcripts show evidence of an extended 3’UTR in testis and most also have the extended 3’ UTR in the other tissues as well
    Figure Legend Snippet: piRNA clusters mark extended 3’ UTRs. Potential precursors of extended 3’ UTR piRNA clusters are detectable as 3’ UTR isoforms in testis and in somatic tissues. a Representative example of a piRNA cluster that extends beyond an annotated 3’ UTR. The annotated 3’UTR is shown in purple and the piRNA cluster is shown in red. Individual piRNA alignments are in blue (sense strand) and red (antisense strand) at the bottom. Genomic features are visualized using the Integrative Genomics Viewer [ 51 ]. b Unannotated portions of 3’ UTR piRNA clusters are enriched for polyA+ RNA expression when compared to randomly selected unannotated regions. We used an adult testis RNA-Seq dataset [ 18 ] to determine expression of the unannotated portions of the extended 3’ UTR clusters (dark blue) or random intergenic regions of equal size (light blue). This indicates that the unannotated extended 3’UTRs are transcribed in the testis. c Expression of the unannotated portion of extended 3’ UTR clusters (X-axis) compared with expression of the corresponding annotated mRNA (Y-axis) in the adult testis RNA-Seq dataset [ 18 ]. The annotated portions of genes are generally expressed at higher RPKM than the unannotated portion of the 3’UTR. d Schematic of Northern blot probes, RT-PCR primers, and PCR amplicons. e Northern blot of Pdpr with an exonic probe (top) shows bands consistent with the length of the annotated mRNA, plus a band consistent with addition of the unannotated region corresponding to the piRNA cluster. The bands are visible only in brain. Probing of the same blot with the unannotated 3’UTR probe (bottom) shows a single band in the same position as the upper band in the exonic probe blot. f Representative RT-PCR results of the three Pdpr amplicons shown schematically in D and marked to the right, with (+) and without (−) reverse transcriptase during the cDNA synthesis step (top). g Summary of RT-PCR products obtained from 10 transcripts with extended 3’ UTR piRNA clusters. For each gene testis, brain, spleen, and liver were tested. All transcripts show evidence of an extended 3’UTR in testis and most also have the extended 3’ UTR in the other tissues as well

    Techniques Used: RNA Expression, RNA Sequencing Assay, Expressing, Northern Blot, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction

    Related Articles

    RNA HS Assay:

    Article Title: Assessing the utility of the Oxford Nanopore MinION for snake venom gland cDNA sequencing
    Article Snippet: .. mRNA extraction and double-stranded cDNA synthesis Total RNA was extracted from the venom glands of two Echis coloratus (snap-frozen after removal and stored at −80 °C ( ; )) using TriReagent (Sigma T9424; Sigma Aldrich, St. Louis, MO, USA) and mRNA purified using the polyA Spin mRNA Isolation Kit (New England BioLabs S1560; New England BioLabs, Ipswich, MA, USA). mRNA was quantified using a Qubit fluorometer (Qubit RNA HS Assay Kit Q32852; Thermo Scientific, Waltham, MA, USA) and reverse transcription carried out using 120 ng (Eco6) or 240 ng of mRNA (Eco8). .. Primer annealing was performed at 65 °C for 5 min in a 13 µl reaction comprising the required amount of mRNA, 2 µl of 1 µM Oligo d(T)23 VN primer (New England BioLabs S1327S; New England BioLabs, Ipswich, MA, USA), 1 µl of 10 mM dNTPs and the appropriate volume of Rnase-free water.

    Isolation:

    Article Title: The 5? Untranslated Region of Protein Kinase C? Directs Translation by an Internal Ribosome Entry Segment That Is Most Active in Densely Growing Cells and during Apoptosis
    Article Snippet: .. Polyadenylated RNA was purified with oligo(dT) cellulose with the PolyA-spin mRNA isolation kit (New England Biolabs). .. RNA from transiently transfected cells [total: 20 μg of luciferase RNA, 10 μg of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) RNA, or 1 μg of poly(A) RNA] was denatured with formamide/formaldehyde and subjected to electrophoresis on a 1% agarose-6% formaldehyde-morpholinepropanesulfonic acid (MOPS) gel.

    Article Title: A Human-Specific α7-Nicotinic Acetylcholine Receptor Gene in Human Leukocytes: Identification, Regulation and the Consequences of CHRFAM7A Expression
    Article Snippet: .. Three μg total RNA was processed for mRNA using PolyA Spin mRNA Isolation Kit (New England Biolabs [NEB], Ipswich, MA, USA). .. One-fifth of the poly A mRNA was reverse transcribed and the resulting cDNA was amplified sequentially by PCR and nested PCR.

    Article Title: Assessing the utility of the Oxford Nanopore MinION for snake venom gland cDNA sequencing
    Article Snippet: .. mRNA extraction and double-stranded cDNA synthesis Total RNA was extracted from the venom glands of two Echis coloratus (snap-frozen after removal and stored at −80 °C ( ; )) using TriReagent (Sigma T9424; Sigma Aldrich, St. Louis, MO, USA) and mRNA purified using the polyA Spin mRNA Isolation Kit (New England BioLabs S1560; New England BioLabs, Ipswich, MA, USA). mRNA was quantified using a Qubit fluorometer (Qubit RNA HS Assay Kit Q32852; Thermo Scientific, Waltham, MA, USA) and reverse transcription carried out using 120 ng (Eco6) or 240 ng of mRNA (Eco8). .. Primer annealing was performed at 65 °C for 5 min in a 13 µl reaction comprising the required amount of mRNA, 2 µl of 1 µM Oligo d(T)23 VN primer (New England BioLabs S1327S; New England BioLabs, Ipswich, MA, USA), 1 µl of 10 mM dNTPs and the appropriate volume of Rnase-free water.

    Article Title: Interferon-Inducible Ubiquitin E2, Ubc8, Is a Conjugating Enzyme for Protein ISGylation †
    Article Snippet: .. RNA was harvested at 6, 12, 18, and 24 h posttreatment and pooled, and poly(A)+ RNA was isolated with a Poly A+ Spin mRNA isolation kit (New England BioLabs, Beverly, Mass.). .. First-strand cDNA was synthesized by using random priming of 1 μg of poly(A)+ RNA.

    Article Title: piRNA-like small RNAs mark extended 3’UTRs present in germ and somatic cells
    Article Snippet: .. Tissues were homogenized in TRIzol Reagent (Invitrogen) using a Tissue Tearor homogenizer (Biospec Products) and total RNA was purified according to the manufacturer’s instructions. mRNA was purified from total RNA using a polyA Spin mRNA Isolation Kit (New England Biolabs) according to manufacturer’s instructions. .. Northern blotting used the Ambion NorthernMax kit (Invitrogen) following the manufacturer’s instructions.

    Article Title: A Novel Small Heat Shock Protein Gene, vis1, Contributes to Pectin Depolymerization and Juice Viscosity in Tomato Fruit 1
    Article Snippet: .. The poly(A+ ) RNA was purified using PolyA spin mRNA isolation kit (New England Biolabs, Beverly, MA) essentially as described by the manufacturer. cDNA subtraction library was constructed using a PCR Select cDNA subtraction kit (BD Biosciences Clontech, Palo Alto, CA) according to manufacturer's instructions (User Manual PT1117–1). ..

    Article Title: p54nrb/NONO Regulates Cyclic AMP-Dependent Glucocorticoid Production by Modulating Phosphodiesterase mRNA Splicing and Degradation
    Article Snippet: .. Poly(A)+ -enriched RNA was isolated from total RNA using oligo(dT) resin in the form of Dyna l beads according to the kit protocol (PolyA Spin mRNA isolation kit; New England BioLabs, Beverly, MA). .. Nuclear and cytoplasmic RNA samples were extracted by using a Paris kit (Life Technologies) according to the manufacturer's protocol.

    Construct:

    Article Title: A Novel Small Heat Shock Protein Gene, vis1, Contributes to Pectin Depolymerization and Juice Viscosity in Tomato Fruit 1
    Article Snippet: .. The poly(A+ ) RNA was purified using PolyA spin mRNA isolation kit (New England Biolabs, Beverly, MA) essentially as described by the manufacturer. cDNA subtraction library was constructed using a PCR Select cDNA subtraction kit (BD Biosciences Clontech, Palo Alto, CA) according to manufacturer's instructions (User Manual PT1117–1). ..

    Purification:

    Article Title: The 5? Untranslated Region of Protein Kinase C? Directs Translation by an Internal Ribosome Entry Segment That Is Most Active in Densely Growing Cells and during Apoptosis
    Article Snippet: .. Polyadenylated RNA was purified with oligo(dT) cellulose with the PolyA-spin mRNA isolation kit (New England Biolabs). .. RNA from transiently transfected cells [total: 20 μg of luciferase RNA, 10 μg of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) RNA, or 1 μg of poly(A) RNA] was denatured with formamide/formaldehyde and subjected to electrophoresis on a 1% agarose-6% formaldehyde-morpholinepropanesulfonic acid (MOPS) gel.

    Article Title: Assessing the utility of the Oxford Nanopore MinION for snake venom gland cDNA sequencing
    Article Snippet: .. mRNA extraction and double-stranded cDNA synthesis Total RNA was extracted from the venom glands of two Echis coloratus (snap-frozen after removal and stored at −80 °C ( ; )) using TriReagent (Sigma T9424; Sigma Aldrich, St. Louis, MO, USA) and mRNA purified using the polyA Spin mRNA Isolation Kit (New England BioLabs S1560; New England BioLabs, Ipswich, MA, USA). mRNA was quantified using a Qubit fluorometer (Qubit RNA HS Assay Kit Q32852; Thermo Scientific, Waltham, MA, USA) and reverse transcription carried out using 120 ng (Eco6) or 240 ng of mRNA (Eco8). .. Primer annealing was performed at 65 °C for 5 min in a 13 µl reaction comprising the required amount of mRNA, 2 µl of 1 µM Oligo d(T)23 VN primer (New England BioLabs S1327S; New England BioLabs, Ipswich, MA, USA), 1 µl of 10 mM dNTPs and the appropriate volume of Rnase-free water.

    Article Title: piRNA-like small RNAs mark extended 3’UTRs present in germ and somatic cells
    Article Snippet: .. Tissues were homogenized in TRIzol Reagent (Invitrogen) using a Tissue Tearor homogenizer (Biospec Products) and total RNA was purified according to the manufacturer’s instructions. mRNA was purified from total RNA using a polyA Spin mRNA Isolation Kit (New England Biolabs) according to manufacturer’s instructions. .. Northern blotting used the Ambion NorthernMax kit (Invitrogen) following the manufacturer’s instructions.

    Article Title: A Novel Small Heat Shock Protein Gene, vis1, Contributes to Pectin Depolymerization and Juice Viscosity in Tomato Fruit 1
    Article Snippet: .. The poly(A+ ) RNA was purified using PolyA spin mRNA isolation kit (New England Biolabs, Beverly, MA) essentially as described by the manufacturer. cDNA subtraction library was constructed using a PCR Select cDNA subtraction kit (BD Biosciences Clontech, Palo Alto, CA) according to manufacturer's instructions (User Manual PT1117–1). ..

    other:

    Article Title: Analysis of Plasmodium vivax schizont transcriptomes from field isolates reveals heterogeneity of expression of genes involved in host-parasite interactions
    Article Snippet: Briefly, polyA+ RNA (mRNA) was selected using magnetic oligo-d(T) beads.

    Polymerase Chain Reaction:

    Article Title: A Novel Small Heat Shock Protein Gene, vis1, Contributes to Pectin Depolymerization and Juice Viscosity in Tomato Fruit 1
    Article Snippet: .. The poly(A+ ) RNA was purified using PolyA spin mRNA isolation kit (New England Biolabs, Beverly, MA) essentially as described by the manufacturer. cDNA subtraction library was constructed using a PCR Select cDNA subtraction kit (BD Biosciences Clontech, Palo Alto, CA) according to manufacturer's instructions (User Manual PT1117–1). ..

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    New England Biolabs polya spin mrna isolation kit
    piRNA clusters mark extended 3’ UTRs. Potential precursors of extended 3’ UTR piRNA clusters are detectable as 3’ UTR isoforms in testis and in somatic tissues. a Representative example of a piRNA cluster that extends beyond an annotated 3’ UTR. The annotated 3’UTR is shown in purple and the piRNA cluster is shown in red. Individual piRNA alignments are in blue (sense strand) and red (antisense strand) at the bottom. Genomic features are visualized using the Integrative Genomics Viewer [ 51 ]. b Unannotated portions of 3’ UTR piRNA clusters are enriched for <t>polyA+</t> RNA expression when compared to randomly selected unannotated regions. We used an adult testis RNA-Seq dataset [ 18 ] to determine expression of the unannotated portions of the extended 3’ UTR clusters (dark blue) or random intergenic regions of equal size (light blue). This indicates that the unannotated extended 3’UTRs are transcribed in the testis. c Expression of the unannotated portion of extended 3’ UTR clusters (X-axis) compared with expression of the corresponding annotated <t>mRNA</t> (Y-axis) in the adult testis RNA-Seq dataset [ 18 ]. The annotated portions of genes are generally expressed at higher RPKM than the unannotated portion of the 3’UTR. d Schematic of Northern blot probes, RT-PCR primers, and PCR amplicons. e Northern blot of Pdpr with an exonic probe (top) shows bands consistent with the length of the annotated mRNA, plus a band consistent with addition of the unannotated region corresponding to the piRNA cluster. The bands are visible only in brain. Probing of the same blot with the unannotated 3’UTR probe (bottom) shows a single band in the same position as the upper band in the exonic probe blot. f Representative RT-PCR results of the three Pdpr amplicons shown schematically in D and marked to the right, with (+) and without (−) reverse transcriptase during the cDNA synthesis step (top). g Summary of RT-PCR products obtained from 10 transcripts with extended 3’ UTR piRNA clusters. For each gene testis, brain, spleen, and liver were tested. All transcripts show evidence of an extended 3’UTR in testis and most also have the extended 3’ UTR in the other tissues as well
    Polya Spin Mrna Isolation Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polya spin mrna isolation kit/product/New England Biolabs
    Average 97 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    polya spin mrna isolation kit - by Bioz Stars, 2020-08
    97/100 stars
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    piRNA clusters mark extended 3’ UTRs. Potential precursors of extended 3’ UTR piRNA clusters are detectable as 3’ UTR isoforms in testis and in somatic tissues. a Representative example of a piRNA cluster that extends beyond an annotated 3’ UTR. The annotated 3’UTR is shown in purple and the piRNA cluster is shown in red. Individual piRNA alignments are in blue (sense strand) and red (antisense strand) at the bottom. Genomic features are visualized using the Integrative Genomics Viewer [ 51 ]. b Unannotated portions of 3’ UTR piRNA clusters are enriched for polyA+ RNA expression when compared to randomly selected unannotated regions. We used an adult testis RNA-Seq dataset [ 18 ] to determine expression of the unannotated portions of the extended 3’ UTR clusters (dark blue) or random intergenic regions of equal size (light blue). This indicates that the unannotated extended 3’UTRs are transcribed in the testis. c Expression of the unannotated portion of extended 3’ UTR clusters (X-axis) compared with expression of the corresponding annotated mRNA (Y-axis) in the adult testis RNA-Seq dataset [ 18 ]. The annotated portions of genes are generally expressed at higher RPKM than the unannotated portion of the 3’UTR. d Schematic of Northern blot probes, RT-PCR primers, and PCR amplicons. e Northern blot of Pdpr with an exonic probe (top) shows bands consistent with the length of the annotated mRNA, plus a band consistent with addition of the unannotated region corresponding to the piRNA cluster. The bands are visible only in brain. Probing of the same blot with the unannotated 3’UTR probe (bottom) shows a single band in the same position as the upper band in the exonic probe blot. f Representative RT-PCR results of the three Pdpr amplicons shown schematically in D and marked to the right, with (+) and without (−) reverse transcriptase during the cDNA synthesis step (top). g Summary of RT-PCR products obtained from 10 transcripts with extended 3’ UTR piRNA clusters. For each gene testis, brain, spleen, and liver were tested. All transcripts show evidence of an extended 3’UTR in testis and most also have the extended 3’ UTR in the other tissues as well

    Journal: BMC Genomics

    Article Title: piRNA-like small RNAs mark extended 3’UTRs present in germ and somatic cells

    doi: 10.1186/s12864-015-1662-6

    Figure Lengend Snippet: piRNA clusters mark extended 3’ UTRs. Potential precursors of extended 3’ UTR piRNA clusters are detectable as 3’ UTR isoforms in testis and in somatic tissues. a Representative example of a piRNA cluster that extends beyond an annotated 3’ UTR. The annotated 3’UTR is shown in purple and the piRNA cluster is shown in red. Individual piRNA alignments are in blue (sense strand) and red (antisense strand) at the bottom. Genomic features are visualized using the Integrative Genomics Viewer [ 51 ]. b Unannotated portions of 3’ UTR piRNA clusters are enriched for polyA+ RNA expression when compared to randomly selected unannotated regions. We used an adult testis RNA-Seq dataset [ 18 ] to determine expression of the unannotated portions of the extended 3’ UTR clusters (dark blue) or random intergenic regions of equal size (light blue). This indicates that the unannotated extended 3’UTRs are transcribed in the testis. c Expression of the unannotated portion of extended 3’ UTR clusters (X-axis) compared with expression of the corresponding annotated mRNA (Y-axis) in the adult testis RNA-Seq dataset [ 18 ]. The annotated portions of genes are generally expressed at higher RPKM than the unannotated portion of the 3’UTR. d Schematic of Northern blot probes, RT-PCR primers, and PCR amplicons. e Northern blot of Pdpr with an exonic probe (top) shows bands consistent with the length of the annotated mRNA, plus a band consistent with addition of the unannotated region corresponding to the piRNA cluster. The bands are visible only in brain. Probing of the same blot with the unannotated 3’UTR probe (bottom) shows a single band in the same position as the upper band in the exonic probe blot. f Representative RT-PCR results of the three Pdpr amplicons shown schematically in D and marked to the right, with (+) and without (−) reverse transcriptase during the cDNA synthesis step (top). g Summary of RT-PCR products obtained from 10 transcripts with extended 3’ UTR piRNA clusters. For each gene testis, brain, spleen, and liver were tested. All transcripts show evidence of an extended 3’UTR in testis and most also have the extended 3’ UTR in the other tissues as well

    Article Snippet: Tissues were homogenized in TRIzol Reagent (Invitrogen) using a Tissue Tearor homogenizer (Biospec Products) and total RNA was purified according to the manufacturer’s instructions. mRNA was purified from total RNA using a polyA Spin mRNA Isolation Kit (New England Biolabs) according to manufacturer’s instructions.

    Techniques: RNA Expression, RNA Sequencing Assay, Expressing, Northern Blot, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction