12 tube magnetic separation rack  (New England Biolabs)


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    Name:
    12 Tube Magnetic Separation Rack
    Description:
    12 Tube Magnetic Separation Rack
    Catalog Number:
    s1509s
    Price:
    333
    Category:
    Magnetic Separation Equipment
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    Structured Review

    New England Biolabs 12 tube magnetic separation rack
    12 Tube Magnetic Separation Rack
    12 Tube Magnetic Separation Rack
    https://www.bioz.com/result/12 tube magnetic separation rack/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    12 tube magnetic separation rack - by Bioz Stars, 2021-03
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Long non-coding RNA Meg3 deficiency impairs glucose homeostasis and insulin signaling by inducing cellular senescence of hepatic endothelium in obesity
    Article Snippet: Cell slurry was transferred onto a 100 μm cell strainer pre-wet with equal volume of wash medium (DMEM/F12 medium with 10%FBS), then span at 500×g for 10 min. Supernatant was gently aspirated and discarded, and cell pellet was re-suspended in 10 ml wash medium followed by second filtering with 40 μm cell strainer and spinning at 500×g for 10 min. Supernatant was gently aspirated and cell pellet was mixed with 1 ml incubation buffer (0.1% BSA, 2 mM EDTA and 0.5% FBS in 1 x dPBS). .. Cell suspension was incubated with sheep anti-rat IgG Dynabeads (Thermo Fisher, Cat# 11035) coated with PECAM-1 antibodies (Becton Dickinson Co, Cat# 557355) and tumbled at 4 °C for 15 min. After incubation, beads were separated by using magnetic separation rack (NEB, Cat# S1509S), and washed with 1 ml of 0.1% BSA in PBS twice, then mixed with 500 μl TRIzol reagent for each sample followed by RNA extraction according to the manufacturer's instructions. .. 2.6 Senescence-associated β-gal stainingHUVECs were seeded into two T25 flasks (750,000 cells/flask), and transfected with 10 nM negative control or Meg3 gapmeRs using Lipofectamine 2000 on the next day.

    Article Title: Spontaneous Glycan Reattachment Following N-Glycanase Treatment of Influenza and HIV Vaccine Antigens.
    Article Snippet: In cells, asparagine/N-linked glycans are added to glycoproteins cotranslationally, in an attachment process that supports proper folding of the nascent polypeptide. .. In cells, asparagine/N-linked glycans are added to glycoproteins cotranslationally, in an attachment process that supports proper folding of the nascent polypeptide. .. In cells, asparagine/N-linked glycans are added to glycoproteins cotranslationally, in an attachment process that supports proper folding of the nascent polypeptide.

    Article Title: Spontaneous glycan reattachment following N-glycanase treatment of influenza and HIV vaccine antigens
    Article Snippet: Purified influenza H1 HA trimer was first de-N-glycosylated under non-denaturing conditions using commercial PNGase F tagged with a chitin-binding domain (CBD) (Remove-iT® PNGase F, Cat# P0706S, New England BioLabs) (=CBD-PNGase F) according to the manufacturer’s instructions (0.72 mg/mL HA, 40 000 units/mL CBD-PNGase F, 50 mM Na3 PO4 , pH 7.5; 24 h incubation at 37°C). .. Chitin magnetic beads (Cat# E8036S, New England BioLabs) equilibrated CBD binding buffer (500 mM NaCl, 20 mM Tris-HCI, 1 mM EDTA, pH 8) were incubated with the deglycosylation reaction (1 hr at 4°C, RotoFlex Plus Tube Rotator, Argos Technologies) and then applied to deplete CBD-PNGase from solution via separation on a magnetic column (NEB cat. S1509), as per the manufacturer’s instructions. .. To ensure separation of HA and CBD-PNGase F, the reaction was further separated on a Superdex 200 10/300 size exclusion column (SEC) using an automated FPLC system (Acta pure 2L, GE).

    RNA Extraction:

    Article Title: Long non-coding RNA Meg3 deficiency impairs glucose homeostasis and insulin signaling by inducing cellular senescence of hepatic endothelium in obesity
    Article Snippet: Cell slurry was transferred onto a 100 μm cell strainer pre-wet with equal volume of wash medium (DMEM/F12 medium with 10%FBS), then span at 500×g for 10 min. Supernatant was gently aspirated and discarded, and cell pellet was re-suspended in 10 ml wash medium followed by second filtering with 40 μm cell strainer and spinning at 500×g for 10 min. Supernatant was gently aspirated and cell pellet was mixed with 1 ml incubation buffer (0.1% BSA, 2 mM EDTA and 0.5% FBS in 1 x dPBS). .. Cell suspension was incubated with sheep anti-rat IgG Dynabeads (Thermo Fisher, Cat# 11035) coated with PECAM-1 antibodies (Becton Dickinson Co, Cat# 557355) and tumbled at 4 °C for 15 min. After incubation, beads were separated by using magnetic separation rack (NEB, Cat# S1509S), and washed with 1 ml of 0.1% BSA in PBS twice, then mixed with 500 μl TRIzol reagent for each sample followed by RNA extraction according to the manufacturer's instructions. .. 2.6 Senescence-associated β-gal stainingHUVECs were seeded into two T25 flasks (750,000 cells/flask), and transfected with 10 nM negative control or Meg3 gapmeRs using Lipofectamine 2000 on the next day.

    Magnetic Beads:

    Article Title: Spontaneous Glycan Reattachment Following N-Glycanase Treatment of Influenza and HIV Vaccine Antigens.
    Article Snippet: In cells, asparagine/N-linked glycans are added to glycoproteins cotranslationally, in an attachment process that supports proper folding of the nascent polypeptide. .. In cells, asparagine/N-linked glycans are added to glycoproteins cotranslationally, in an attachment process that supports proper folding of the nascent polypeptide. .. In cells, asparagine/N-linked glycans are added to glycoproteins cotranslationally, in an attachment process that supports proper folding of the nascent polypeptide.

    Article Title: Spontaneous glycan reattachment following N-glycanase treatment of influenza and HIV vaccine antigens
    Article Snippet: Purified influenza H1 HA trimer was first de-N-glycosylated under non-denaturing conditions using commercial PNGase F tagged with a chitin-binding domain (CBD) (Remove-iT® PNGase F, Cat# P0706S, New England BioLabs) (=CBD-PNGase F) according to the manufacturer’s instructions (0.72 mg/mL HA, 40 000 units/mL CBD-PNGase F, 50 mM Na3 PO4 , pH 7.5; 24 h incubation at 37°C). .. Chitin magnetic beads (Cat# E8036S, New England BioLabs) equilibrated CBD binding buffer (500 mM NaCl, 20 mM Tris-HCI, 1 mM EDTA, pH 8) were incubated with the deglycosylation reaction (1 hr at 4°C, RotoFlex Plus Tube Rotator, Argos Technologies) and then applied to deplete CBD-PNGase from solution via separation on a magnetic column (NEB cat. S1509), as per the manufacturer’s instructions. .. To ensure separation of HA and CBD-PNGase F, the reaction was further separated on a Superdex 200 10/300 size exclusion column (SEC) using an automated FPLC system (Acta pure 2L, GE).

    Binding Assay:

    Article Title: Spontaneous Glycan Reattachment Following N-Glycanase Treatment of Influenza and HIV Vaccine Antigens.
    Article Snippet: In cells, asparagine/N-linked glycans are added to glycoproteins cotranslationally, in an attachment process that supports proper folding of the nascent polypeptide. .. In cells, asparagine/N-linked glycans are added to glycoproteins cotranslationally, in an attachment process that supports proper folding of the nascent polypeptide. .. In cells, asparagine/N-linked glycans are added to glycoproteins cotranslationally, in an attachment process that supports proper folding of the nascent polypeptide.

    Article Title: Spontaneous glycan reattachment following N-glycanase treatment of influenza and HIV vaccine antigens
    Article Snippet: Purified influenza H1 HA trimer was first de-N-glycosylated under non-denaturing conditions using commercial PNGase F tagged with a chitin-binding domain (CBD) (Remove-iT® PNGase F, Cat# P0706S, New England BioLabs) (=CBD-PNGase F) according to the manufacturer’s instructions (0.72 mg/mL HA, 40 000 units/mL CBD-PNGase F, 50 mM Na3 PO4 , pH 7.5; 24 h incubation at 37°C). .. Chitin magnetic beads (Cat# E8036S, New England BioLabs) equilibrated CBD binding buffer (500 mM NaCl, 20 mM Tris-HCI, 1 mM EDTA, pH 8) were incubated with the deglycosylation reaction (1 hr at 4°C, RotoFlex Plus Tube Rotator, Argos Technologies) and then applied to deplete CBD-PNGase from solution via separation on a magnetic column (NEB cat. S1509), as per the manufacturer’s instructions. .. To ensure separation of HA and CBD-PNGase F, the reaction was further separated on a Superdex 200 10/300 size exclusion column (SEC) using an automated FPLC system (Acta pure 2L, GE).

    Polyacrylamide Gel Electrophoresis:

    Article Title: Spontaneous Glycan Reattachment Following N-Glycanase Treatment of Influenza and HIV Vaccine Antigens.
    Article Snippet: In cells, asparagine/N-linked glycans are added to glycoproteins cotranslationally, in an attachment process that supports proper folding of the nascent polypeptide. .. In cells, asparagine/N-linked glycans are added to glycoproteins cotranslationally, in an attachment process that supports proper folding of the nascent polypeptide. .. In cells, asparagine/N-linked glycans are added to glycoproteins cotranslationally, in an attachment process that supports proper folding of the nascent polypeptide.

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  • 94
    New England Biolabs 12 tube magnetic separation rack
    12 Tube Magnetic Separation Rack, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/12 tube magnetic separation rack/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    12 tube magnetic separation rack - by Bioz Stars, 2021-03
    94/100 stars
      Buy from Supplier

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