goat anti rat igg magnetic beads  (New England Biolabs)


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    Name:
    Goat Anti Rat IgG Magnetic Beads
    Description:
    Goat Anti Rat IgG Magnetic Beads 20 mg
    Catalog Number:
    s1433s
    Price:
    186
    Size:
    20 mg
    Category:
    Magnetic Separation Equipment
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    Structured Review

    New England Biolabs goat anti rat igg magnetic beads
    Goat Anti Rat IgG Magnetic Beads
    Goat Anti Rat IgG Magnetic Beads 20 mg
    https://www.bioz.com/result/goat anti rat igg magnetic beads/product/New England Biolabs
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    goat anti rat igg magnetic beads - by Bioz Stars, 2020-03
    99/100 stars

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    Clone Assay:

    Article Title: The BCL-2 pro-survival protein A1 is dispensable for T cell homeostasis on viral infection
    Article Snippet: For the enrichment of CD8+ CTLs from spleens of infected mice, splenocytes were incubated with supernatants from hybridoma clones producing the following mABs (WEHI, Antibody Facility, Melbourne, VIC, Australia): rat anti-B220, rat anti-CD11b, rat anti-GR-1, rat anti-CD4, and rat anti-MHCII. .. Spleen cells expressing these surface markers were depleted using goat anti-rat IgG antibody conjugated magnetic beads (NEB, Ipswich, MA, USA, Cat# S1433S).

    Cell Isolation:

    Article Title: Transcription factor repertoire of homeostatic eosinophilopoiesis
    Article Snippet: Paragraph title: Cell isolation and identification ... Goat anti-rat IgG magnetic beads (New England BioLabs, Inc.) were prepared according to the manufacturer’s instructions and coated with anti-mouse B220 (clone RA3.3A1/6.1), CD19 (clone 1D3), or Ly6-G (clone 1A8) (BioXCell, 1 μL of beads per μg of antibody).

    Centrifugation:

    Article Title: Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Article Snippet: After centrifugation, cell pellets were resuspended in PBS with 2% bovine calf serum, blocked with rat IgG for 10 min, and stained with rat anti-mouse CD31 APC (eBioscience, 17-0311-80, 1:500) and anti-mouse CD45 (Biolegend, 103101, 1:200). .. To analyze Ino80 and Vegfr2 mRNA in sorted populations from e14.5 hearts cells were sorted using antibody-coupled magnetic beads, Purified Rat Anti-Mouse CD144 (BD Pharmaceutical, 550548) and Goat Anti-Rat IgG Magnetic Beads (NEB, S1433S).).

    Article Title: Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Article Snippet: After centrifugation, cell pellets were resuspended in PBS with 2% bovine calf serum, blocked with rat IgG for 10 min, and stained with rat anti-mouse CD31 APC (eBioscience, 17-0311-80, 1:500) and anti-mouse CD45 (Biolegend, 103101, 1:200). .. To analyze Ino80 and Vegfr2 mRNA in sorted populations from e14.5 hearts cells were sorted using antibody-coupled magnetic beads, Purified Rat Anti-Mouse CD144 (BD Pharmaceutical, 550548) and Goat Anti-Rat IgG Magnetic Beads (NEB, S1433S).).

    Quantitative RT-PCR:

    Article Title: Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Article Snippet: To analyze Ino80 and Vegfr2 mRNA in sorted populations from e14.5 hearts cells were sorted using antibody-coupled magnetic beads, Purified Rat Anti-Mouse CD144 (BD Pharmaceutical, 550548) and Goat Anti-Rat IgG Magnetic Beads (NEB, S1433S).). .. The SYBR Green qPCR master mix (BIO-RAD, 1725121) was used and quantitative RT–PCR was performed on Real-Time PCR System (Bio-Rad, CFX96 Touch Real-Time PCR dectection system).

    Article Title: Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Article Snippet: To analyze Ino80 and Vegfr2 mRNA in sorted populations from e14.5 hearts cells were sorted using antibody-coupled magnetic beads, Purified Rat Anti-Mouse CD144 (BD Pharmaceutical, 550548) and Goat Anti-Rat IgG Magnetic Beads (NEB, S1433S).). .. The SYBR Green qPCR master mix (BIO-RAD, 1725121) was used and quantitative RT–PCR was performed on Real-Time PCR System (Bio-Rad, CFX96 Touch Real-Time PCR dectection system).

    Real-time Polymerase Chain Reaction:

    Article Title: Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Article Snippet: To analyze Ino80 and Vegfr2 mRNA in sorted populations from e14.5 hearts cells were sorted using antibody-coupled magnetic beads, Purified Rat Anti-Mouse CD144 (BD Pharmaceutical, 550548) and Goat Anti-Rat IgG Magnetic Beads (NEB, S1433S).). .. The SYBR Green qPCR master mix (BIO-RAD, 1725121) was used and quantitative RT–PCR was performed on Real-Time PCR System (Bio-Rad, CFX96 Touch Real-Time PCR dectection system).

    Article Title: Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Article Snippet: To analyze Ino80 and Vegfr2 mRNA in sorted populations from e14.5 hearts cells were sorted using antibody-coupled magnetic beads, Purified Rat Anti-Mouse CD144 (BD Pharmaceutical, 550548) and Goat Anti-Rat IgG Magnetic Beads (NEB, S1433S).). .. The SYBR Green qPCR master mix (BIO-RAD, 1725121) was used and quantitative RT–PCR was performed on Real-Time PCR System (Bio-Rad, CFX96 Touch Real-Time PCR dectection system).

    Incubation:

    Article Title: Transcription factor repertoire of homeostatic eosinophilopoiesis
    Article Snippet: Goat anti-rat IgG magnetic beads (New England BioLabs, Inc.) were prepared according to the manufacturer’s instructions and coated with anti-mouse B220 (clone RA3.3A1/6.1), CD19 (clone 1D3), or Ly6-G (clone 1A8) (BioXCell, 1 μL of beads per μg of antibody). .. The beads were added to the cell suspension at a 5:1 ratio and incubated at least 1 h at 4°C with gentle agitation on a rotating plate.

    Article Title: Gtpbp2 is a positive regulator of Wnt signaling and maintains low levels of the Wnt negative regulator Axin
    Article Snippet: .. Cell lysates were incubated with mouse anti-Myc (9E10) antibody overnight, purified using anti-IgG magnetic beads (NEB) according to the manufacturers instructions. ..

    Article Title: The BCL-2 pro-survival protein A1 is dispensable for T cell homeostasis on viral infection
    Article Snippet: For the enrichment of CD8+ CTLs from spleens of infected mice, splenocytes were incubated with supernatants from hybridoma clones producing the following mABs (WEHI, Antibody Facility, Melbourne, VIC, Australia): rat anti-B220, rat anti-CD11b, rat anti-GR-1, rat anti-CD4, and rat anti-MHCII. .. Spleen cells expressing these surface markers were depleted using goat anti-rat IgG antibody conjugated magnetic beads (NEB, Ipswich, MA, USA, Cat# S1433S).

    Article Title: Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Article Snippet: Isolation of heart endothelial cells Hearts from Tie2Cre;Ino80 fl/fl and Tie2Cre;Ino80 fl/+ embryos were collected at e15.5, minced with a sterilized razor blade, and incubated in 0.5 ml dissociation solution (collagenase, dispase, and DNase) at 37 °C for 20 min. Once digested, 10 ml of PBS with 2% bovine calf serum was added and the digested heart tissues were filtered through 40 µm cell strainer. .. To analyze Ino80 and Vegfr2 mRNA in sorted populations from e14.5 hearts cells were sorted using antibody-coupled magnetic beads, Purified Rat Anti-Mouse CD144 (BD Pharmaceutical, 550548) and Goat Anti-Rat IgG Magnetic Beads (NEB, S1433S).).

    Article Title: Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Article Snippet: Hearts from Tie2Cre;Ino80 fl/fl and Tie2Cre;Ino80 fl/+ embryos were collected at e15.5, minced with a sterilized razor blade, and incubated in 0.5 ml dissociation solution (collagenase, dispase, and DNase) at 37 °C for 20 min. Once digested, 10 ml of PBS with 2% bovine calf serum was added and the digested heart tissues were filtered through 40 µm cell strainer. .. To analyze Ino80 and Vegfr2 mRNA in sorted populations from e14.5 hearts cells were sorted using antibody-coupled magnetic beads, Purified Rat Anti-Mouse CD144 (BD Pharmaceutical, 550548) and Goat Anti-Rat IgG Magnetic Beads (NEB, S1433S).).

    Article Title: Increased peripheral IL-4 leads to an expanded virtual memory CD8+ population
    Article Snippet: For H2Kb tetramer staining, single-cell suspensions of combined spleen and lymph nodes were enriched for CD8+ T cells through negative selection using rat anti-mouse I-A/I-E (M5/114.15.2) and goat anti-rat IgG magnetic beads (New England Biolabs) according to the manufacturer’s protocol. .. Cells were then incubated on ice for 1 hour, washed twice with FACS buffer and stained 25 min. for surface antigens.

    Expressing:

    Article Title: The BCL-2 pro-survival protein A1 is dispensable for T cell homeostasis on viral infection
    Article Snippet: .. Spleen cells expressing these surface markers were depleted using goat anti-rat IgG antibody conjugated magnetic beads (NEB, Ipswich, MA, USA, Cat# S1433S). .. Enrichment efficiency was assessed by flow cytometry and 2 × 104 NP-specific CTLs were injected in 200 μ l PBS i.v. into Ly5.1 wild-type recipient mice.

    Western Blot:

    Article Title: Gtpbp2 is a positive regulator of Wnt signaling and maintains low levels of the Wnt negative regulator Axin
    Article Snippet: Paragraph title: Immunoprecipitation and western blotting ... Cell lysates were incubated with mouse anti-Myc (9E10) antibody overnight, purified using anti-IgG magnetic beads (NEB) according to the manufacturers instructions.

    Flow Cytometry:

    Article Title: The BCL-2 pro-survival protein A1 is dispensable for T cell homeostasis on viral infection
    Article Snippet: Spleen cells expressing these surface markers were depleted using goat anti-rat IgG antibody conjugated magnetic beads (NEB, Ipswich, MA, USA, Cat# S1433S). .. Enrichment efficiency was assessed by flow cytometry and 2 × 104 NP-specific CTLs were injected in 200 μ l PBS i.v. into Ly5.1 wild-type recipient mice.

    Article Title: Increased peripheral IL-4 leads to an expanded virtual memory CD8+ population
    Article Snippet: Paragraph title: Flow cytometry ... For H2Kb tetramer staining, single-cell suspensions of combined spleen and lymph nodes were enriched for CD8+ T cells through negative selection using rat anti-mouse I-A/I-E (M5/114.15.2) and goat anti-rat IgG magnetic beads (New England Biolabs) according to the manufacturer’s protocol.

    Infection:

    Article Title: The BCL-2 pro-survival protein A1 is dispensable for T cell homeostasis on viral infection
    Article Snippet: For the enrichment of CD8+ CTLs from spleens of infected mice, splenocytes were incubated with supernatants from hybridoma clones producing the following mABs (WEHI, Antibody Facility, Melbourne, VIC, Australia): rat anti-B220, rat anti-CD11b, rat anti-GR-1, rat anti-CD4, and rat anti-MHCII. .. Spleen cells expressing these surface markers were depleted using goat anti-rat IgG antibody conjugated magnetic beads (NEB, Ipswich, MA, USA, Cat# S1433S).

    Injection:

    Article Title: The BCL-2 pro-survival protein A1 is dispensable for T cell homeostasis on viral infection
    Article Snippet: Spleen cells expressing these surface markers were depleted using goat anti-rat IgG antibody conjugated magnetic beads (NEB, Ipswich, MA, USA, Cat# S1433S). .. Enrichment efficiency was assessed by flow cytometry and 2 × 104 NP-specific CTLs were injected in 200 μ l PBS i.v. into Ly5.1 wild-type recipient mice.

    Magnetic Beads:

    Article Title: Transcription factor repertoire of homeostatic eosinophilopoiesis
    Article Snippet: .. Goat anti-rat IgG magnetic beads (New England BioLabs, Inc.) were prepared according to the manufacturer’s instructions and coated with anti-mouse B220 (clone RA3.3A1/6.1), CD19 (clone 1D3), or Ly6-G (clone 1A8) (BioXCell, 1 μL of beads per μg of antibody). .. The beads were added to the cell suspension at a 5:1 ratio and incubated at least 1 h at 4°C with gentle agitation on a rotating plate.

    Article Title: Gtpbp2 is a positive regulator of Wnt signaling and maintains low levels of the Wnt negative regulator Axin
    Article Snippet: .. Cell lysates were incubated with mouse anti-Myc (9E10) antibody overnight, purified using anti-IgG magnetic beads (NEB) according to the manufacturers instructions. ..

    Article Title: The BCL-2 pro-survival protein A1 is dispensable for T cell homeostasis on viral infection
    Article Snippet: .. Spleen cells expressing these surface markers were depleted using goat anti-rat IgG antibody conjugated magnetic beads (NEB, Ipswich, MA, USA, Cat# S1433S). .. Enrichment efficiency was assessed by flow cytometry and 2 × 104 NP-specific CTLs were injected in 200 μ l PBS i.v. into Ly5.1 wild-type recipient mice.

    Article Title: Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Article Snippet: .. To analyze Ino80 and Vegfr2 mRNA in sorted populations from e14.5 hearts cells were sorted using antibody-coupled magnetic beads, Purified Rat Anti-Mouse CD144 (BD Pharmaceutical, 550548) and Goat Anti-Rat IgG Magnetic Beads (NEB, S1433S).). .. Total RNA was extracted according to the manufacturer’s instructions (Qiagen, 74034) and converted to cDNA using iScript RT Supermix (BIO-RAD, 1708840).

    Article Title: Thermochemiluminescent Semiconducting Polymer Dots as sensitive nanoprobe for reagentless immunoassay
    Article Snippet: .. Cyano-polyphenylene vinylene (CN-PPV, MW= 20 - 100 kDa) was purchased from American Dye Source, Inc. while polystyrene derivatives (PS-COOH, 50 kDa and PS-NH2 , 6.5 kDa) was purchased from Polymer Source, Inc. Biotinylated Mouse IgG was purchased from Abcam, while Anti-IgG magnetic beads were purchased from New England BioLabs, Inc. .. Substrate 1 was synthesized following a previously reported procedure (for more details see ).

    Article Title: Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Article Snippet: .. To analyze Ino80 and Vegfr2 mRNA in sorted populations from e14.5 hearts cells were sorted using antibody-coupled magnetic beads, Purified Rat Anti-Mouse CD144 (BD Pharmaceutical, 550548) and Goat Anti-Rat IgG Magnetic Beads (NEB, S1433S).). .. Total RNA was extracted according to the manufacturer’s instructions (Qiagen, 74034) and converted to cDNA using iScript RT Supermix (BIO-RAD, 1708840).

    Article Title: Increased peripheral IL-4 leads to an expanded virtual memory CD8+ population
    Article Snippet: .. For H2Kb tetramer staining, single-cell suspensions of combined spleen and lymph nodes were enriched for CD8+ T cells through negative selection using rat anti-mouse I-A/I-E (M5/114.15.2) and goat anti-rat IgG magnetic beads (New England Biolabs) according to the manufacturer’s protocol. .. Tetramer was added after blocking with Fcblock.

    Mutagenesis:

    Article Title: A functional analysis of TOEFAZ1 uncovers protein domains essential for cytokinesis in Trypanosoma brucei
    Article Snippet: The Δ IDP domain mutant cell line was treated with either 70% ethanol or 1 µg ml−1 doxycycline to induce TOEFAZ1 RNAi for 3 days. .. 50 µl of anti-rat IgG magnetic bead slurry (NEB) was added to each condition and rotated for 45 min RT.

    Isolation:

    Article Title: Transcription factor repertoire of homeostatic eosinophilopoiesis
    Article Snippet: Bone marrow cells were isolated as previously described ( ). .. Goat anti-rat IgG magnetic beads (New England BioLabs, Inc.) were prepared according to the manufacturer’s instructions and coated with anti-mouse B220 (clone RA3.3A1/6.1), CD19 (clone 1D3), or Ly6-G (clone 1A8) (BioXCell, 1 μL of beads per μg of antibody).

    Article Title: Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Article Snippet: Paragraph title: Isolation of heart endothelial cells ... To analyze Ino80 and Vegfr2 mRNA in sorted populations from e14.5 hearts cells were sorted using antibody-coupled magnetic beads, Purified Rat Anti-Mouse CD144 (BD Pharmaceutical, 550548) and Goat Anti-Rat IgG Magnetic Beads (NEB, S1433S).).

    Article Title: Increased peripheral IL-4 leads to an expanded virtual memory CD8+ population
    Article Snippet: Single-cell suspensions of lymphocytes isolated from spleen or thymus were stained for 10 min. on ice with live/dead stain, blocked for 5 min. with Fcblock, then stained a further 25 min. with antibodies directed against surface antigens. .. For H2Kb tetramer staining, single-cell suspensions of combined spleen and lymph nodes were enriched for CD8+ T cells through negative selection using rat anti-mouse I-A/I-E (M5/114.15.2) and goat anti-rat IgG magnetic beads (New England Biolabs) according to the manufacturer’s protocol.

    Mouse Assay:

    Article Title: The BCL-2 pro-survival protein A1 is dispensable for T cell homeostasis on viral infection
    Article Snippet: For the enrichment of CD8+ CTLs from spleens of infected mice, splenocytes were incubated with supernatants from hybridoma clones producing the following mABs (WEHI, Antibody Facility, Melbourne, VIC, Australia): rat anti-B220, rat anti-CD11b, rat anti-GR-1, rat anti-CD4, and rat anti-MHCII. .. Spleen cells expressing these surface markers were depleted using goat anti-rat IgG antibody conjugated magnetic beads (NEB, Ipswich, MA, USA, Cat# S1433S).

    Cytometry:

    Article Title: The BCL-2 pro-survival protein A1 is dispensable for T cell homeostasis on viral infection
    Article Snippet: Spleen cells expressing these surface markers were depleted using goat anti-rat IgG antibody conjugated magnetic beads (NEB, Ipswich, MA, USA, Cat# S1433S). .. Enrichment efficiency was assessed by flow cytometry and 2 × 104 NP-specific CTLs were injected in 200 μ l PBS i.v. into Ly5.1 wild-type recipient mice.

    Article Title: Increased peripheral IL-4 leads to an expanded virtual memory CD8+ population
    Article Snippet: Paragraph title: Flow cytometry ... For H2Kb tetramer staining, single-cell suspensions of combined spleen and lymph nodes were enriched for CD8+ T cells through negative selection using rat anti-mouse I-A/I-E (M5/114.15.2) and goat anti-rat IgG magnetic beads (New England Biolabs) according to the manufacturer’s protocol.

    FACS:

    Article Title: Transcription factor repertoire of homeostatic eosinophilopoiesis
    Article Snippet: Goat anti-rat IgG magnetic beads (New England BioLabs, Inc.) were prepared according to the manufacturer’s instructions and coated with anti-mouse B220 (clone RA3.3A1/6.1), CD19 (clone 1D3), or Ly6-G (clone 1A8) (BioXCell, 1 μL of beads per μg of antibody). .. Cells were stained for 15 min at room temperature in FACS buffer (1X PBS, 0.5% FBS, and 2 mM EDTA).

    Article Title: Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Article Snippet: Viable CD45-negative, CD31-positive cells were sorted on BD FACS Aria and collected into 0.5 ml of Trizol LS reagent (Invitrogen, 10296028) directly for RNA extraction. .. To analyze Ino80 and Vegfr2 mRNA in sorted populations from e14.5 hearts cells were sorted using antibody-coupled magnetic beads, Purified Rat Anti-Mouse CD144 (BD Pharmaceutical, 550548) and Goat Anti-Rat IgG Magnetic Beads (NEB, S1433S).).

    Article Title: Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Article Snippet: Viable CD45-negative, CD31-positive cells were sorted on BD FACS Aria and collected into 0.5 ml of Trizol LS reagent (Invitrogen, 10296028) directly for RNA extraction. .. To analyze Ino80 and Vegfr2 mRNA in sorted populations from e14.5 hearts cells were sorted using antibody-coupled magnetic beads, Purified Rat Anti-Mouse CD144 (BD Pharmaceutical, 550548) and Goat Anti-Rat IgG Magnetic Beads (NEB, S1433S).).

    Article Title: Increased peripheral IL-4 leads to an expanded virtual memory CD8+ population
    Article Snippet: Cells were then washed twice with PBS plus sodium azide plus FCS (FACS buffer). .. For H2Kb tetramer staining, single-cell suspensions of combined spleen and lymph nodes were enriched for CD8+ T cells through negative selection using rat anti-mouse I-A/I-E (M5/114.15.2) and goat anti-rat IgG magnetic beads (New England Biolabs) according to the manufacturer’s protocol.

    Blocking Assay:

    Article Title: Increased peripheral IL-4 leads to an expanded virtual memory CD8+ population
    Article Snippet: For H2Kb tetramer staining, single-cell suspensions of combined spleen and lymph nodes were enriched for CD8+ T cells through negative selection using rat anti-mouse I-A/I-E (M5/114.15.2) and goat anti-rat IgG magnetic beads (New England Biolabs) according to the manufacturer’s protocol. .. Tetramer was added after blocking with Fcblock.

    Lysis:

    Article Title: Gtpbp2 is a positive regulator of Wnt signaling and maintains low levels of the Wnt negative regulator Axin
    Article Snippet: Cells were incubated overnight and lysed in NP40 lysis buffer (10 mM Tris-Cl pH 8.0, 137 mM NaCl, 10 % Glycerol, 1 % NP-40, + complete protease and phosphatase inhibitor tablets; Roche). .. Cell lysates were incubated with mouse anti-Myc (9E10) antibody overnight, purified using anti-IgG magnetic beads (NEB) according to the manufacturers instructions.

    Article Title: A functional analysis of TOEFAZ1 uncovers protein domains essential for cytokinesis in Trypanosoma brucei
    Article Snippet: 2.5×108 cells from each condition were harvested, washed with PBS, and resuspended in 650 µl of cold lysis buffer (10 mM NaPO4 , 150 mM NaCl, 0.5% glucose, 7.0% sucrose, and 0.5% NP-40 with protease and phosphatase inhibitors). .. 50 µl of anti-rat IgG magnetic bead slurry (NEB) was added to each condition and rotated for 45 min RT.

    Purification:

    Article Title: Gtpbp2 is a positive regulator of Wnt signaling and maintains low levels of the Wnt negative regulator Axin
    Article Snippet: .. Cell lysates were incubated with mouse anti-Myc (9E10) antibody overnight, purified using anti-IgG magnetic beads (NEB) according to the manufacturers instructions. ..

    Article Title: Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Article Snippet: .. To analyze Ino80 and Vegfr2 mRNA in sorted populations from e14.5 hearts cells were sorted using antibody-coupled magnetic beads, Purified Rat Anti-Mouse CD144 (BD Pharmaceutical, 550548) and Goat Anti-Rat IgG Magnetic Beads (NEB, S1433S).). .. Total RNA was extracted according to the manufacturer’s instructions (Qiagen, 74034) and converted to cDNA using iScript RT Supermix (BIO-RAD, 1708840).

    Article Title: Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Article Snippet: .. To analyze Ino80 and Vegfr2 mRNA in sorted populations from e14.5 hearts cells were sorted using antibody-coupled magnetic beads, Purified Rat Anti-Mouse CD144 (BD Pharmaceutical, 550548) and Goat Anti-Rat IgG Magnetic Beads (NEB, S1433S).). .. Total RNA was extracted according to the manufacturer’s instructions (Qiagen, 74034) and converted to cDNA using iScript RT Supermix (BIO-RAD, 1708840).

    SDS Page:

    Article Title: Gtpbp2 is a positive regulator of Wnt signaling and maintains low levels of the Wnt negative regulator Axin
    Article Snippet: Cell lysates were incubated with mouse anti-Myc (9E10) antibody overnight, purified using anti-IgG magnetic beads (NEB) according to the manufacturers instructions. .. For western blotting Xenopus embryos or explants were lysed in NP40 lysis buffer and extracted with an equal volume of 1,1,2-trichlorotrifluoro-ethane (Sigma), and then separated using SDS-page.

    Article Title: A functional analysis of TOEFAZ1 uncovers protein domains essential for cytokinesis in Trypanosoma brucei
    Article Snippet: 50 µl of anti-rat IgG magnetic bead slurry (NEB) was added to each condition and rotated for 45 min RT. .. The beads were then washed three times in lysis buffer and bound proteins were eluted in SDS-PAGE loading buffer as described above.

    SYBR Green Assay:

    Article Title: Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Article Snippet: To analyze Ino80 and Vegfr2 mRNA in sorted populations from e14.5 hearts cells were sorted using antibody-coupled magnetic beads, Purified Rat Anti-Mouse CD144 (BD Pharmaceutical, 550548) and Goat Anti-Rat IgG Magnetic Beads (NEB, S1433S).). .. The SYBR Green qPCR master mix (BIO-RAD, 1725121) was used and quantitative RT–PCR was performed on Real-Time PCR System (Bio-Rad, CFX96 Touch Real-Time PCR dectection system).

    Article Title: Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Article Snippet: To analyze Ino80 and Vegfr2 mRNA in sorted populations from e14.5 hearts cells were sorted using antibody-coupled magnetic beads, Purified Rat Anti-Mouse CD144 (BD Pharmaceutical, 550548) and Goat Anti-Rat IgG Magnetic Beads (NEB, S1433S).). .. The SYBR Green qPCR master mix (BIO-RAD, 1725121) was used and quantitative RT–PCR was performed on Real-Time PCR System (Bio-Rad, CFX96 Touch Real-Time PCR dectection system).

    RNA Extraction:

    Article Title: Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Article Snippet: Viable CD45-negative, CD31-positive cells were sorted on BD FACS Aria and collected into 0.5 ml of Trizol LS reagent (Invitrogen, 10296028) directly for RNA extraction. .. To analyze Ino80 and Vegfr2 mRNA in sorted populations from e14.5 hearts cells were sorted using antibody-coupled magnetic beads, Purified Rat Anti-Mouse CD144 (BD Pharmaceutical, 550548) and Goat Anti-Rat IgG Magnetic Beads (NEB, S1433S).).

    Article Title: Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Article Snippet: Viable CD45-negative, CD31-positive cells were sorted on BD FACS Aria and collected into 0.5 ml of Trizol LS reagent (Invitrogen, 10296028) directly for RNA extraction. .. To analyze Ino80 and Vegfr2 mRNA in sorted populations from e14.5 hearts cells were sorted using antibody-coupled magnetic beads, Purified Rat Anti-Mouse CD144 (BD Pharmaceutical, 550548) and Goat Anti-Rat IgG Magnetic Beads (NEB, S1433S).).

    Selection:

    Article Title: Increased peripheral IL-4 leads to an expanded virtual memory CD8+ population
    Article Snippet: .. For H2Kb tetramer staining, single-cell suspensions of combined spleen and lymph nodes were enriched for CD8+ T cells through negative selection using rat anti-mouse I-A/I-E (M5/114.15.2) and goat anti-rat IgG magnetic beads (New England Biolabs) according to the manufacturer’s protocol. .. Tetramer was added after blocking with Fcblock.

    Immunoprecipitation:

    Article Title: Gtpbp2 is a positive regulator of Wnt signaling and maintains low levels of the Wnt negative regulator Axin
    Article Snippet: Paragraph title: Immunoprecipitation and western blotting ... Cell lysates were incubated with mouse anti-Myc (9E10) antibody overnight, purified using anti-IgG magnetic beads (NEB) according to the manufacturers instructions.

    Article Title: A functional analysis of TOEFAZ1 uncovers protein domains essential for cytokinesis in Trypanosoma brucei
    Article Snippet: Paragraph title: Immunoprecipitation of TbPLK ... 50 µl of anti-rat IgG magnetic bead slurry (NEB) was added to each condition and rotated for 45 min RT.

    Staining:

    Article Title: Transcription factor repertoire of homeostatic eosinophilopoiesis
    Article Snippet: Goat anti-rat IgG magnetic beads (New England BioLabs, Inc.) were prepared according to the manufacturer’s instructions and coated with anti-mouse B220 (clone RA3.3A1/6.1), CD19 (clone 1D3), or Ly6-G (clone 1A8) (BioXCell, 1 μL of beads per μg of antibody). .. Cells were stained for 15 min at room temperature in FACS buffer (1X PBS, 0.5% FBS, and 2 mM EDTA).

    Article Title: Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Article Snippet: After centrifugation, cell pellets were resuspended in PBS with 2% bovine calf serum, blocked with rat IgG for 10 min, and stained with rat anti-mouse CD31 APC (eBioscience, 17-0311-80, 1:500) and anti-mouse CD45 (Biolegend, 103101, 1:200). .. To analyze Ino80 and Vegfr2 mRNA in sorted populations from e14.5 hearts cells were sorted using antibody-coupled magnetic beads, Purified Rat Anti-Mouse CD144 (BD Pharmaceutical, 550548) and Goat Anti-Rat IgG Magnetic Beads (NEB, S1433S).).

    Article Title: Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
    Article Snippet: After centrifugation, cell pellets were resuspended in PBS with 2% bovine calf serum, blocked with rat IgG for 10 min, and stained with rat anti-mouse CD31 APC (eBioscience, 17-0311-80, 1:500) and anti-mouse CD45 (Biolegend, 103101, 1:200). .. To analyze Ino80 and Vegfr2 mRNA in sorted populations from e14.5 hearts cells were sorted using antibody-coupled magnetic beads, Purified Rat Anti-Mouse CD144 (BD Pharmaceutical, 550548) and Goat Anti-Rat IgG Magnetic Beads (NEB, S1433S).).

    Article Title: Increased peripheral IL-4 leads to an expanded virtual memory CD8+ population
    Article Snippet: .. For H2Kb tetramer staining, single-cell suspensions of combined spleen and lymph nodes were enriched for CD8+ T cells through negative selection using rat anti-mouse I-A/I-E (M5/114.15.2) and goat anti-rat IgG magnetic beads (New England Biolabs) according to the manufacturer’s protocol. .. Tetramer was added after blocking with Fcblock.

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  • 99
    New England Biolabs goat anti rat igg magnetic beads
    Goat Anti Rat Igg Magnetic Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rat igg magnetic beads/product/New England Biolabs
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    goat anti rat igg magnetic beads - by Bioz Stars, 2020-03
    99/100 stars
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