goat anti rabbit magnetic beads  (New England Biolabs)


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    Name:
    Goat Anti Rabbit IgG Magnetic Beads
    Description:
    Goat Anti Rabbit IgG Magnetic Beads 20 mg
    Catalog Number:
    s1432s
    Price:
    188
    Size:
    20 mg
    Category:
    Magnetic Separation Equipment
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    Structured Review

    New England Biolabs goat anti rabbit magnetic beads
    Goat Anti Rabbit IgG Magnetic Beads
    Goat Anti Rabbit IgG Magnetic Beads 20 mg
    https://www.bioz.com/result/goat anti rabbit magnetic beads/product/New England Biolabs
    Average 95 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit magnetic beads - by Bioz Stars, 2020-02
    95/100 stars

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    Transduction:

    Article Title: Migration of nerve growth cones requires detergent-resistant membranes in a spatially defined and substrate-dependent manner
    Article Snippet: Antibodies Mouse monoclonal anti-TfR antibody was obtained from Zymed Laboratories, rabbit polyclonal anti-Ncad antibody and goat polyclonal anti-CTxB antiserum from Calbiochem, rabbit polyclonal anti-actin antiserum from Sigma-Aldrich, mouse monoclonal anti-β1 integrin antibody from Transduction Laboratories, rat monoclonal anti-β1 integrin antibody from CHEMICON International, and rat monoclonal anti-Thy-1 from BD Biosciences. .. HRP-conjugated goat anti–mouse IgG and goat anti–rabbit IgG were purchased from New England Biolabs, Inc., HRP-conjugated goat anti–rat IgG from Cappel, and Alexa-conjugated secondary antibodies from Molecular Probes, Inc.

    Blocking Assay:

    Article Title: Cloning and Immunocytochemical Localization of a Cyclic Nucleotide–Gated Channel α-Subunit to All Cone Photoreceptors in the Mouse Retina
    Article Snippet: After blocking in 5% (wt/vol) powdered milk in Tris-buffered saline (TBS: 0.05 M Tris-HCl, pH 7.5, 0.15 M NaCl) containing 0.05% (vol/vol) Tween-20 (TBST) for 1–3 hours at room temperature, the membranes were incubated in the peptide antiserum (1: 2,000) overnight. .. After five washes for 5 minutes each in TBST, the blots were incubated in goat anti-rabbit IgG conjugated to horseradish peroxidase (New England Bio-labs, 1:2,000) in 5% milk in TBST for 0.5–1 hour.

    Incubation:

    Article Title: N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication
    Article Snippet: .. The mixture was then incubated with 20 μl of anti-rabbit antibody conjugated magnetic beads (NEB, Ipswich, MA, USA; S1432S), which were then washed three times with 500 μl of IP buffer, followed by rotating for 2 h at 4°C. .. Beads were washed six times with 500 μl of IP buffer and then incubated with 300 μl of elution buffer (5 mM Tris–HCl, pH 7.5, 1 mM EDTA, pH 8.0, 0.05% sodium dodecyl sulfate (SDS), and 4.2 μl of 20 mg/ml proteinase K) for 1.5 h at 50°C.

    Article Title: Hemoglobin Promotes Staphylococcus aureus Nasal Colonization
    Article Snippet: .. Hemoglobin was removed from indicated samples and nasal secretions by adding 2 µl anti-hemoglobin antibody produced in rabbit (Sigma) with a 1 hour incubation, followed by addition of 5 µg of goat anti-rabbit IgG Magnetic Beads (New England Biolabs) with a 1 hour incubation at room temperature with periodic rocking. ..

    Article Title: Gene Expression and Stress Response Mediated by the Epigenetic Regulation of a Transposable Element Small RNA
    Article Snippet: .. In a standard immunoprecipitation reaction, Arabidopsis protein extract was pre-cleared by incubation with 10 µl of goat anti-rabbit magnetic beads (NEB). .. Pre-cleared extracts were then incubated overnight with goat anti-rabbit magnetic beads pre-incubated with 1 µg α-AGO1.

    Article Title: Cloning and Immunocytochemical Localization of a Cyclic Nucleotide–Gated Channel α-Subunit to All Cone Photoreceptors in the Mouse Retina
    Article Snippet: .. After five washes for 5 minutes each in TBST, the blots were incubated in goat anti-rabbit IgG conjugated to horseradish peroxidase (New England Bio-labs, 1:2,000) in 5% milk in TBST for 0.5–1 hour. .. Following the same wash protocol, the signal was developed using enhanced chemiluminescence (SuperSignal Substrate, Pierce).

    Article Title: Fetal lung C4BPA induces p100 processing in human placenta
    Article Snippet: Immunoprecipitation of exosomes We coupled goat-anti-rabbit immunomagnetic beads (New England Biolabs, USA) coated with anti-AFP or C4BPA antibody by mixing them and rotating at 4 °C for 1 hr. .. The antibody-bound beads were incubated with the total exosome at 4 °C for 1 hr with bidirectional mixing.

    Article Title: Zinc metallothionein imported into liver mitochondria modulates respiration
    Article Snippet: The membrane was blocked with 5% (wt/vol) fat-free milk for 3 h at room temperature and washed as described above, and purified anti-MT-3 antibody [1:5,000 dilution in TBST buffer containing 2.5% (wt/vol) BSA and 2.5% (wt/vol) fat-free milk] was added, and then incubated overnight at room temperature. .. After three washes, the secondary antibody, goat anti-rabbit IgG coupled to horseradish peroxidase (New England Biolabs), was used.

    Article Title: The Role of the Trypanosoma cruzi TcNRBD1 Protein in Translation
    Article Snippet: .. Immunoprecipitation–Proteomics Twenty microliters of anti-NRBD1 were incubated with 50 μL of anti-rabbit IgG magnetic beads (New England Biolabs) for 2 hours at room temperature with continuous orbital shaking. .. The preimmune serum was incubated with the beads in the same conditions as a control for the specificity of the immunoprecipitation reaction.

    Article Title: Comparative study of naturally occurring huntingtin fragments in Drosophila points to exon 1 as the most pathogenic species in Huntington's disease
    Article Snippet: .. 100 μg of magnetic beads (S1432S NEB, binding capacity 10 μg) conjugated to anti-rabbit IgG secondary antibody were washed with PBS buffer and incubated with a solution of 10 μg of A11 antibody (AB9234, Millipore) in 1 ml of 0.1% BSA in PBS buffer at RT with continuous shaking at low speed. .. After a 2-h incubation, magnetic beads were removed and washed three times with PBST buffer (1 ml) and resuspended in 1 ml of PBS.

    Article Title: Therapeutic Molecules and Endogenous Ligands Regulate the Interaction between Brain Cellular Prion Protein (PrPC) and Metabotropic Glutamate Receptor 5 (mGluR5)
    Article Snippet: One microgram of capture antibody was incubated overnight at 4 °C with 1 mg of detergent-solubilized lysate protein with continuous mixing. .. PureProteome protein A/G mix magnetic beads (Millipore, catalog no. LSKMAGAG10) or goat anti-rabbit IgG magnetic beads (New England Biolabs, catalog no. S1432S) were washed in wash buffer (PBS and 0.1% Tween 20 (pH 7.4)).

    Article Title: Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi
    Article Snippet: .. EF-1α immunoprecipitation RNA-seq To identify mRNAs associated with EF-1α protein or complexes, rabbit anti-EF-1α antibody (15 μl) was incubated with 50 μl of goat anti-rabbit magnetic beads (New England Biolabs) and 40 U/ml RNAse OUT (Invitrogen) for 16 h at 4 °C with shaking. .. Cytoplasmic extract corresponding to 1 × 109 cells was then incubated with the beads.

    Article Title: ZNT7 binds to CD40 and influences CD154‐triggered p38 MAPK activity in B lymphocytes—a possible regulatory mechanism for zinc in immune function
    Article Snippet: .. Goat anti‐(rabbit IgG) magnetic beads (200 μg, New England BioLabs, Ipswich, MA, USA) were added and incubated at RT for another 30 min. .. The precleared lysate was then incubated with a ZNT7 antibody (1 μg) at RT for 1 h. Prewashed magnetic beads (200 μg) were then added and incubated overnight at 4 °C.

    Expressing:

    Article Title: Comparative study of naturally occurring huntingtin fragments in Drosophila points to exon 1 as the most pathogenic species in Huntington's disease
    Article Snippet: Extracts of Drosophila expressing different N-terminal HTT peptides were tested for their ability to seed the formation of thioflavinT-positive amyloid using an ASA ( ) with modifications. .. 100 μg of magnetic beads (S1432S NEB, binding capacity 10 μg) conjugated to anti-rabbit IgG secondary antibody were washed with PBS buffer and incubated with a solution of 10 μg of A11 antibody (AB9234, Millipore) in 1 ml of 0.1% BSA in PBS buffer at RT with continuous shaking at low speed.

    Modification:

    Article Title: Ratanhiaphenol III from Ratanhiae Radix is a PTP1B Inhibitor
    Article Snippet: Dulbecco’s modified essential medium (DMEM) was purchased from Lonza, and fetal calf serum, horse serum, penicillin, streptomycin, glutamine, and trypsin was from Gibco/Invitrogen. .. Secondary antibodies: goat anti-rabbit IgG was from New England Biolabs and goat anti-mouse IgG from Upstate.

    Western Blot:

    Article Title: Cloning and Immunocytochemical Localization of a Cyclic Nucleotide–Gated Channel α-Subunit to All Cone Photoreceptors in the Mouse Retina
    Article Snippet: Paragraph title: Western blot analysis ... After five washes for 5 minutes each in TBST, the blots were incubated in goat anti-rabbit IgG conjugated to horseradish peroxidase (New England Bio-labs, 1:2,000) in 5% milk in TBST for 0.5–1 hour.

    Article Title: Zinc metallothionein imported into liver mitochondria modulates respiration
    Article Snippet: It was further purified by precipitation with an acetone extract of rat liver cellular protein ( ) and used in Western blots to detect MT-3. .. After three washes, the secondary antibody, goat anti-rabbit IgG coupled to horseradish peroxidase (New England Biolabs), was used.

    Article Title: Migration of nerve growth cones requires detergent-resistant membranes in a spatially defined and substrate-dependent manner
    Article Snippet: Rabbit antiserum against the L1 cytoplasmic domain ( ) was provided by Dr. Vance Lemmon (Case Western Reserve University, Cleveland, OH). .. HRP-conjugated goat anti–mouse IgG and goat anti–rabbit IgG were purchased from New England Biolabs, Inc., HRP-conjugated goat anti–rat IgG from Cappel, and Alexa-conjugated secondary antibodies from Molecular Probes, Inc.

    Article Title: ZNT7 binds to CD40 and influences CD154‐triggered p38 MAPK activity in B lymphocytes—a possible regulatory mechanism for zinc in immune function
    Article Snippet: Goat anti‐(rabbit IgG) magnetic beads (200 μg, New England BioLabs, Ipswich, MA, USA) were added and incubated at RT for another 30 min. .. The protein complex was released from the beads in 2× protein loading buffer (BioRad) at 99 °C for 10 min followed by western blot analysis.

    High Performance Liquid Chromatography:

    Article Title: Ratanhiaphenol III from Ratanhiae Radix is a PTP1B Inhibitor
    Article Snippet: Secondary antibodies: goat anti-rabbit IgG was from New England Biolabs and goat anti-mouse IgG from Upstate. .. All solvents and additives used for analysis and isolation were of HPLC-grade and purchased from Merck.

    Produced:

    Article Title: Hemoglobin Promotes Staphylococcus aureus Nasal Colonization
    Article Snippet: .. Hemoglobin was removed from indicated samples and nasal secretions by adding 2 µl anti-hemoglobin antibody produced in rabbit (Sigma) with a 1 hour incubation, followed by addition of 5 µg of goat anti-rabbit IgG Magnetic Beads (New England Biolabs) with a 1 hour incubation at room temperature with periodic rocking. ..

    Article Title: Ratanhiaphenol III from Ratanhiae Radix is a PTP1B Inhibitor
    Article Snippet: Secondary antibodies: goat anti-rabbit IgG was from New England Biolabs and goat anti-mouse IgG from Upstate. .. Ultrapure water was produced by a Sartorius Arium® 611 UV water purification system.

    Immunoprecipitation:

    Article Title: N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication
    Article Snippet: Paragraph title: m6 A-Methylated RNA immunoprecipitation (MeRIP) and Northern blotting ... The mixture was then incubated with 20 μl of anti-rabbit antibody conjugated magnetic beads (NEB, Ipswich, MA, USA; S1432S), which were then washed three times with 500 μl of IP buffer, followed by rotating for 2 h at 4°C.

    Article Title: Gene Expression and Stress Response Mediated by the Epigenetic Regulation of a Transposable Element Small RNA
    Article Snippet: .. In a standard immunoprecipitation reaction, Arabidopsis protein extract was pre-cleared by incubation with 10 µl of goat anti-rabbit magnetic beads (NEB). .. Pre-cleared extracts were then incubated overnight with goat anti-rabbit magnetic beads pre-incubated with 1 µg α-AGO1.

    Article Title: Fetal lung C4BPA induces p100 processing in human placenta
    Article Snippet: .. Immunoprecipitation of exosomes We coupled goat-anti-rabbit immunomagnetic beads (New England Biolabs, USA) coated with anti-AFP or C4BPA antibody by mixing them and rotating at 4 °C for 1 hr. .. The beads were washed three times with immunoprecipitation buffer (IP buffer; 10 mM Tris [pH 7.2], 10 mM MgCl2 , 200 mM KCl, 0.1% Triton X-100).

    Article Title: The Role of the Trypanosoma cruzi TcNRBD1 Protein in Translation
    Article Snippet: .. Immunoprecipitation–Proteomics Twenty microliters of anti-NRBD1 were incubated with 50 μL of anti-rabbit IgG magnetic beads (New England Biolabs) for 2 hours at room temperature with continuous orbital shaking. .. The preimmune serum was incubated with the beads in the same conditions as a control for the specificity of the immunoprecipitation reaction.

    Article Title: Therapeutic Molecules and Endogenous Ligands Regulate the Interaction between Brain Cellular Prion Protein (PrPC) and Metabotropic Glutamate Receptor 5 (mGluR5)
    Article Snippet: Paragraph title: Immunoprecipitation ... PureProteome protein A/G mix magnetic beads (Millipore, catalog no. LSKMAGAG10) or goat anti-rabbit IgG magnetic beads (New England Biolabs, catalog no. S1432S) were washed in wash buffer (PBS and 0.1% Tween 20 (pH 7.4)).

    Article Title: Nrf2 Transcription Factor Can Directly Regulate mTOR
    Article Snippet: For each reaction, 400 μg of chromatin and 20 μl of ChIP-Grade Protein G Magnetic Beads (New England BioLabs, catalog #9006S) was used for immunoprecipitation with antibodies against Nrf2 (H300: rabbit Nrf2 antibody, Santa Cruz Biotechnology, Dallas, TX), H3-K27me3 (New England BioLabs; catalog #9733, lot #8), H3-K4-me3 (New England BioLabs, catalog #9751P, lot 9), and p65 (New England BioLabs; catalog #8242P). .. Normal rabbit IgG antibody (New England BioLabs; catalog #2729S, lot #7) was used as a negative control.

    Article Title: Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi
    Article Snippet: .. EF-1α immunoprecipitation RNA-seq To identify mRNAs associated with EF-1α protein or complexes, rabbit anti-EF-1α antibody (15 μl) was incubated with 50 μl of goat anti-rabbit magnetic beads (New England Biolabs) and 40 U/ml RNAse OUT (Invitrogen) for 16 h at 4 °C with shaking. .. Cytoplasmic extract corresponding to 1 × 109 cells was then incubated with the beads.

    Article Title: ZNT7 binds to CD40 and influences CD154‐triggered p38 MAPK activity in B lymphocytes—a possible regulatory mechanism for zinc in immune function
    Article Snippet: Paragraph title: Immunoprecipitation ... Goat anti‐(rabbit IgG) magnetic beads (200 μg, New England BioLabs, Ipswich, MA, USA) were added and incubated at RT for another 30 min.

    Protease Inhibitor:

    Article Title: Gene Expression and Stress Response Mediated by the Epigenetic Regulation of a Transposable Element Small RNA
    Article Snippet: Inflorescence tissue was ground with liquid nitrogen and homogenized in 2 ml extraction buffer (100 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM EDTA, and 5 mM DTT) containing 1 tablet/10 mL protease inhibitor cocktail (Roche) per gram of tissue. .. In a standard immunoprecipitation reaction, Arabidopsis protein extract was pre-cleared by incubation with 10 µl of goat anti-rabbit magnetic beads (NEB).

    Article Title: Ratanhiaphenol III from Ratanhiae Radix is a PTP1B Inhibitor
    Article Snippet: Complete™ protease inhibitor was from Roche Diagnostics, and PVDF membranes were from Bio-Rad Laboratories. .. Secondary antibodies: goat anti-rabbit IgG was from New England Biolabs and goat anti-mouse IgG from Upstate.

    Article Title: Cloning and Immunocytochemical Localization of a Cyclic Nucleotide–Gated Channel α-Subunit to All Cone Photoreceptors in the Mouse Retina
    Article Snippet: Tissues were homogenized in buffer containing 0.32 M sucrose, 0.02 M Tris-HCl, pH 7.4, 5 mM EGTA, and protease inhibitor cocktail (Complete mini, Boehringer Mannheim, Mannheim, Germany). .. After five washes for 5 minutes each in TBST, the blots were incubated in goat anti-rabbit IgG conjugated to horseradish peroxidase (New England Bio-labs, 1:2,000) in 5% milk in TBST for 0.5–1 hour.

    Article Title: Comparative study of naturally occurring huntingtin fragments in Drosophila points to exon 1 as the most pathogenic species in Huntington's disease
    Article Snippet: Briefly, 20 Drosophila L3 larvae were ground in 600 µl PBS supplemented with 1 m m PMSF, 1 m m EDTA, 1 m m DTT and 0.1 m m protease inhibitor cocktail (Sigma P8340) and then further homogenized by the addition of 1-mm glass beads and vortexing at a high speed for 5 min. .. 100 μg of magnetic beads (S1432S NEB, binding capacity 10 μg) conjugated to anti-rabbit IgG secondary antibody were washed with PBS buffer and incubated with a solution of 10 μg of A11 antibody (AB9234, Millipore) in 1 ml of 0.1% BSA in PBS buffer at RT with continuous shaking at low speed.

    Northern Blot:

    Article Title: N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication
    Article Snippet: Paragraph title: m6 A-Methylated RNA immunoprecipitation (MeRIP) and Northern blotting ... The mixture was then incubated with 20 μl of anti-rabbit antibody conjugated magnetic beads (NEB, Ipswich, MA, USA; S1432S), which were then washed three times with 500 μl of IP buffer, followed by rotating for 2 h at 4°C.

    Infection:

    Article Title: N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication
    Article Snippet: m6 A-Methylated RNA immunoprecipitation (MeRIP) and Northern blotting Total RNA was extracted from Vero cells infected with strain EV71-XF at a multiplicity of infection (MOI) of 0.1 using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). .. The mixture was then incubated with 20 μl of anti-rabbit antibody conjugated magnetic beads (NEB, Ipswich, MA, USA; S1432S), which were then washed three times with 500 μl of IP buffer, followed by rotating for 2 h at 4°C.

    Protein Concentration:

    Article Title: ZNT7 binds to CD40 and influences CD154‐triggered p38 MAPK activity in B lymphocytes—a possible regulatory mechanism for zinc in immune function
    Article Snippet: Protein concentration was determined by a bicinchoninic acid assay (BioRad). .. Goat anti‐(rabbit IgG) magnetic beads (200 μg, New England BioLabs, Ipswich, MA, USA) were added and incubated at RT for another 30 min.

    Sonication:

    Article Title: Nrf2 Transcription Factor Can Directly Regulate mTOR
    Article Snippet: To obtain DNA fragments having sizes from 200 to 1000 bp, lysates were subjected to 15 rounds of sonication. .. Normal rabbit IgG antibody (New England BioLabs; catalog #2729S, lot #7) was used as a negative control.

    Binding Assay:

    Article Title: Comparative study of naturally occurring huntingtin fragments in Drosophila points to exon 1 as the most pathogenic species in Huntington's disease
    Article Snippet: .. 100 μg of magnetic beads (S1432S NEB, binding capacity 10 μg) conjugated to anti-rabbit IgG secondary antibody were washed with PBS buffer and incubated with a solution of 10 μg of A11 antibody (AB9234, Millipore) in 1 ml of 0.1% BSA in PBS buffer at RT with continuous shaking at low speed. .. After a 2-h incubation, magnetic beads were removed and washed three times with PBST buffer (1 ml) and resuspended in 1 ml of PBS.

    Molecular Weight:

    Article Title: Cloning and Immunocytochemical Localization of a Cyclic Nucleotide–Gated Channel α-Subunit to All Cone Photoreceptors in the Mouse Retina
    Article Snippet: After five washes for 5 minutes each in TBST, the blots were incubated in goat anti-rabbit IgG conjugated to horseradish peroxidase (New England Bio-labs, 1:2,000) in 5% milk in TBST for 0.5–1 hour. .. Western blots of retina protein showed that the mCNG3 antiserum recognized a single band with an apparent molecular weight of ≈70 kDa, close to the calculated molecular weight of 73 kDa ( ).

    Nucleic Acid Electrophoresis:

    Article Title: Cloning and Immunocytochemical Localization of a Cyclic Nucleotide–Gated Channel α-Subunit to All Cone Photoreceptors in the Mouse Retina
    Article Snippet: Twenty micrograms protein was separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis on an 8% gel and was transferred to nitrocellulose using standard techniques. .. After five washes for 5 minutes each in TBST, the blots were incubated in goat anti-rabbit IgG conjugated to horseradish peroxidase (New England Bio-labs, 1:2,000) in 5% milk in TBST for 0.5–1 hour.

    RNA Sequencing Assay:

    Article Title: Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi
    Article Snippet: .. EF-1α immunoprecipitation RNA-seq To identify mRNAs associated with EF-1α protein or complexes, rabbit anti-EF-1α antibody (15 μl) was incubated with 50 μl of goat anti-rabbit magnetic beads (New England Biolabs) and 40 U/ml RNAse OUT (Invitrogen) for 16 h at 4 °C with shaking. .. Cytoplasmic extract corresponding to 1 × 109 cells was then incubated with the beads.

    Magnetic Beads:

    Article Title: N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication
    Article Snippet: .. The mixture was then incubated with 20 μl of anti-rabbit antibody conjugated magnetic beads (NEB, Ipswich, MA, USA; S1432S), which were then washed three times with 500 μl of IP buffer, followed by rotating for 2 h at 4°C. .. Beads were washed six times with 500 μl of IP buffer and then incubated with 300 μl of elution buffer (5 mM Tris–HCl, pH 7.5, 1 mM EDTA, pH 8.0, 0.05% sodium dodecyl sulfate (SDS), and 4.2 μl of 20 mg/ml proteinase K) for 1.5 h at 50°C.

    Article Title: Hemoglobin Promotes Staphylococcus aureus Nasal Colonization
    Article Snippet: .. Hemoglobin was removed from indicated samples and nasal secretions by adding 2 µl anti-hemoglobin antibody produced in rabbit (Sigma) with a 1 hour incubation, followed by addition of 5 µg of goat anti-rabbit IgG Magnetic Beads (New England Biolabs) with a 1 hour incubation at room temperature with periodic rocking. ..

    Article Title: Gene Expression and Stress Response Mediated by the Epigenetic Regulation of a Transposable Element Small RNA
    Article Snippet: .. In a standard immunoprecipitation reaction, Arabidopsis protein extract was pre-cleared by incubation with 10 µl of goat anti-rabbit magnetic beads (NEB). .. Pre-cleared extracts were then incubated overnight with goat anti-rabbit magnetic beads pre-incubated with 1 µg α-AGO1.

    Article Title: The Role of the Trypanosoma cruzi TcNRBD1 Protein in Translation
    Article Snippet: .. Immunoprecipitation–Proteomics Twenty microliters of anti-NRBD1 were incubated with 50 μL of anti-rabbit IgG magnetic beads (New England Biolabs) for 2 hours at room temperature with continuous orbital shaking. .. The preimmune serum was incubated with the beads in the same conditions as a control for the specificity of the immunoprecipitation reaction.

    Article Title: Comparative study of naturally occurring huntingtin fragments in Drosophila points to exon 1 as the most pathogenic species in Huntington's disease
    Article Snippet: .. 100 μg of magnetic beads (S1432S NEB, binding capacity 10 μg) conjugated to anti-rabbit IgG secondary antibody were washed with PBS buffer and incubated with a solution of 10 μg of A11 antibody (AB9234, Millipore) in 1 ml of 0.1% BSA in PBS buffer at RT with continuous shaking at low speed. .. After a 2-h incubation, magnetic beads were removed and washed three times with PBST buffer (1 ml) and resuspended in 1 ml of PBS.

    Article Title: Therapeutic Molecules and Endogenous Ligands Regulate the Interaction between Brain Cellular Prion Protein (PrPC) and Metabotropic Glutamate Receptor 5 (mGluR5)
    Article Snippet: .. PureProteome protein A/G mix magnetic beads (Millipore, catalog no. LSKMAGAG10) or goat anti-rabbit IgG magnetic beads (New England Biolabs, catalog no. S1432S) were washed in wash buffer (PBS and 0.1% Tween 20 (pH 7.4)). .. The preformed antibody-antigen complex was added to the beads and incubated for 1 h (in the case of HEK-293 cell experiments) or 3 h (in the case of mouse brain experiments) at 4 °C with gentle rotation.

    Article Title: Nrf2 Transcription Factor Can Directly Regulate mTOR
    Article Snippet: For each reaction, 400 μg of chromatin and 20 μl of ChIP-Grade Protein G Magnetic Beads (New England BioLabs, catalog #9006S) was used for immunoprecipitation with antibodies against Nrf2 (H300: rabbit Nrf2 antibody, Santa Cruz Biotechnology, Dallas, TX), H3-K27me3 (New England BioLabs; catalog #9733, lot #8), H3-K4-me3 (New England BioLabs, catalog #9751P, lot 9), and p65 (New England BioLabs; catalog #8242P). .. Normal rabbit IgG antibody (New England BioLabs; catalog #2729S, lot #7) was used as a negative control.

    Article Title: Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi
    Article Snippet: .. EF-1α immunoprecipitation RNA-seq To identify mRNAs associated with EF-1α protein or complexes, rabbit anti-EF-1α antibody (15 μl) was incubated with 50 μl of goat anti-rabbit magnetic beads (New England Biolabs) and 40 U/ml RNAse OUT (Invitrogen) for 16 h at 4 °C with shaking. .. Cytoplasmic extract corresponding to 1 × 109 cells was then incubated with the beads.

    Article Title: ZNT7 binds to CD40 and influences CD154‐triggered p38 MAPK activity in B lymphocytes—a possible regulatory mechanism for zinc in immune function
    Article Snippet: .. Goat anti‐(rabbit IgG) magnetic beads (200 μg, New England BioLabs, Ipswich, MA, USA) were added and incubated at RT for another 30 min. .. The precleared lysate was then incubated with a ZNT7 antibody (1 μg) at RT for 1 h. Prewashed magnetic beads (200 μg) were then added and incubated overnight at 4 °C.

    Isolation:

    Article Title: Gene Expression and Stress Response Mediated by the Epigenetic Regulation of a Transposable Element Small RNA
    Article Snippet: In a standard immunoprecipitation reaction, Arabidopsis protein extract was pre-cleared by incubation with 10 µl of goat anti-rabbit magnetic beads (NEB). .. Immunoprecipitated, mock-immunoprecipitated and input sample RNA was isolated using TRIzol (Invitrogen).

    Article Title: Ratanhiaphenol III from Ratanhiae Radix is a PTP1B Inhibitor
    Article Snippet: Secondary antibodies: goat anti-rabbit IgG was from New England Biolabs and goat anti-mouse IgG from Upstate. .. All solvents and additives used for analysis and isolation were of HPLC-grade and purchased from Merck.

    Purification:

    Article Title: Ratanhiaphenol III from Ratanhiae Radix is a PTP1B Inhibitor
    Article Snippet: Secondary antibodies: goat anti-rabbit IgG was from New England Biolabs and goat anti-mouse IgG from Upstate. .. Ultrapure water was produced by a Sartorius Arium® 611 UV water purification system.

    Article Title: Zinc metallothionein imported into liver mitochondria modulates respiration
    Article Snippet: The membrane was blocked with 5% (wt/vol) fat-free milk for 3 h at room temperature and washed as described above, and purified anti-MT-3 antibody [1:5,000 dilution in TBST buffer containing 2.5% (wt/vol) BSA and 2.5% (wt/vol) fat-free milk] was added, and then incubated overnight at room temperature. .. After three washes, the secondary antibody, goat anti-rabbit IgG coupled to horseradish peroxidase (New England Biolabs), was used.

    Article Title: Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi
    Article Snippet: EF-1α immunoprecipitation RNA-seq To identify mRNAs associated with EF-1α protein or complexes, rabbit anti-EF-1α antibody (15 μl) was incubated with 50 μl of goat anti-rabbit magnetic beads (New England Biolabs) and 40 U/ml RNAse OUT (Invitrogen) for 16 h at 4 °C with shaking. .. The immunoprecipitated RNAs were eluted and purified with the RNeasy® (Qiagen) kit by using the “Animal Cells I” protocol in the manufacturer’s manual but with an additional column-based DNase treatment step.

    Chromatin Immunoprecipitation:

    Article Title: Nrf2 Transcription Factor Can Directly Regulate mTOR
    Article Snippet: Paragraph title: ChIP ... Normal rabbit IgG antibody (New England BioLabs; catalog #2729S, lot #7) was used as a negative control.

    SDS Page:

    Article Title: Zinc metallothionein imported into liver mitochondria modulates respiration
    Article Snippet: The mitochondrial protein fractions were incubated in the SDS sample buffer, boiled for 3 min, and then separated by SDS/PAGE as described above. .. After three washes, the secondary antibody, goat anti-rabbit IgG coupled to horseradish peroxidase (New England Biolabs), was used.

    Plasmid Preparation:

    Article Title: N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication
    Article Snippet: In vitro EV71 RNA was transcribed from a cDNA plasmid ( ) linearized by HindIII using the MEGAscript® T7 Kit (Ambion, Austin, TX, USA) according to the manufacturer's protocols. .. The mixture was then incubated with 20 μl of anti-rabbit antibody conjugated magnetic beads (NEB, Ipswich, MA, USA; S1432S), which were then washed three times with 500 μl of IP buffer, followed by rotating for 2 h at 4°C.

    Negative Control:

    Article Title: Nrf2 Transcription Factor Can Directly Regulate mTOR
    Article Snippet: .. Normal rabbit IgG antibody (New England BioLabs; catalog #2729S, lot #7) was used as a negative control. .. Samples were immunoprecipitated overnight at 4 °C, and the beads were washed 5 times for 5 min with 1 ml of ice-cold immunoprecipitation buffer.

    In Vitro:

    Article Title: N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication
    Article Snippet: For MeRIP, 300 μg of total RNA or 10 μg in vitro transcribed EV71 RNA were incubated with an anti-m6 A antibody (Synaptic Systems, Goettingen, Germany) or a IgG antibody in 300 μl of immunoprecipitation (IP) Buffer (150 mM NaCl, 0.1% NP-40, 10 mM Tris–HCl, pH 7.4) for 2 h at 4°C. .. The mixture was then incubated with 20 μl of anti-rabbit antibody conjugated magnetic beads (NEB, Ipswich, MA, USA; S1432S), which were then washed three times with 500 μl of IP buffer, followed by rotating for 2 h at 4°C.

    Homogenization:

    Article Title: Cloning and Immunocytochemical Localization of a Cyclic Nucleotide–Gated Channel α-Subunit to All Cone Photoreceptors in the Mouse Retina
    Article Snippet: The pellets were resuspended in homogenization buffer, but the sucrose was replaced by 10% glycerol. .. After five washes for 5 minutes each in TBST, the blots were incubated in goat anti-rabbit IgG conjugated to horseradish peroxidase (New England Bio-labs, 1:2,000) in 5% milk in TBST for 0.5–1 hour.

    Subculturing Assay:

    Article Title: Hemoglobin Promotes Staphylococcus aureus Nasal Colonization
    Article Snippet: Hemoglobin was removed from indicated samples and nasal secretions by adding 2 µl anti-hemoglobin antibody produced in rabbit (Sigma) with a 1 hour incubation, followed by addition of 5 µg of goat anti-rabbit IgG Magnetic Beads (New England Biolabs) with a 1 hour incubation at room temperature with periodic rocking. .. The AIP activation assay was performed by growing overnight cultures of S. aureus reporter strain (AH462) in TSB and subculturing 1∶50 into TSB plus 0.2% glucose supplemented with AIP; AIP+hemoglobin; or AIP pretreated with hemoglobin that was removed by antibody pulldown as described above.

    Activation Assay:

    Article Title: Hemoglobin Promotes Staphylococcus aureus Nasal Colonization
    Article Snippet: Paragraph title: Autoinducing peptide activation assays with hemoglobin ... Hemoglobin was removed from indicated samples and nasal secretions by adding 2 µl anti-hemoglobin antibody produced in rabbit (Sigma) with a 1 hour incubation, followed by addition of 5 µg of goat anti-rabbit IgG Magnetic Beads (New England Biolabs) with a 1 hour incubation at room temperature with periodic rocking.

    Acid Assay:

    Article Title: ZNT7 binds to CD40 and influences CD154‐triggered p38 MAPK activity in B lymphocytes—a possible regulatory mechanism for zinc in immune function
    Article Snippet: Protein concentration was determined by a bicinchoninic acid assay (BioRad). .. Goat anti‐(rabbit IgG) magnetic beads (200 μg, New England BioLabs, Ipswich, MA, USA) were added and incubated at RT for another 30 min.

    Concentration Assay:

    Article Title: Hemoglobin Promotes Staphylococcus aureus Nasal Colonization
    Article Snippet: The concentrations were determined with an extinction coefficient of 13,600 M−1 cm−1 , and the prebase reading was subtracted to get the final AIP concentration. .. Hemoglobin was removed from indicated samples and nasal secretions by adding 2 µl anti-hemoglobin antibody produced in rabbit (Sigma) with a 1 hour incubation, followed by addition of 5 µg of goat anti-rabbit IgG Magnetic Beads (New England Biolabs) with a 1 hour incubation at room temperature with periodic rocking.

    Lysis:

    Article Title: The Role of the Trypanosoma cruzi TcNRBD1 Protein in Translation
    Article Snippet: Immunoprecipitation–Proteomics Twenty microliters of anti-NRBD1 were incubated with 50 μL of anti-rabbit IgG magnetic beads (New England Biolabs) for 2 hours at room temperature with continuous orbital shaking. .. The cytoplasmic extracts of T . cruzi obtained from 109 parasites were washed twice with PBS and incubated with 1 ml of hypotonic lysis buffer (50 mM Tris-HCl, pH 7.5, 1 mM MgCl2 , and 150 mM NaCl) for 10 minutes followed by cavitation at 2,000 PSI for 30 minutes.

    Article Title: Nrf2 Transcription Factor Can Directly Regulate mTOR
    Article Snippet: Protease inhibitors, PMSF, and NaF were added directly before lysis. .. Normal rabbit IgG antibody (New England BioLabs; catalog #2729S, lot #7) was used as a negative control.

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  • 95
    New England Biolabs goat anti rabbit magnetic beads
    Goat Anti Rabbit Magnetic Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit magnetic beads/product/New England Biolabs
    Average 95 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit magnetic beads - by Bioz Stars, 2020-02
    95/100 stars
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