anti mouse igg  (New England Biolabs)


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    Name:
    Goat Anti Mouse IgG Magnetic Beads
    Description:
    Goat Anti Mouse IgG Magnetic Beads 20 mg
    Catalog Number:
    s1431s
    Price:
    188
    Size:
    20 mg
    Category:
    Magnetic Separation Equipment
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    Structured Review

    New England Biolabs anti mouse igg
    Goat Anti Mouse IgG Magnetic Beads
    Goat Anti Mouse IgG Magnetic Beads 20 mg
    https://www.bioz.com/result/anti mouse igg/product/New England Biolabs
    Average 94 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    anti mouse igg - by Bioz Stars, 2020-08
    94/100 stars

    Images

    1) Product Images from "Impairing autophagy in retinal pigment epithelium leads to inflammasome activation and enhanced macrophage-mediated angiogenesis"

    Article Title: Impairing autophagy in retinal pigment epithelium leads to inflammasome activation and enhanced macrophage-mediated angiogenesis

    Journal: Scientific Reports

    doi: 10.1038/srep20639

    Contribution of RPE degeneration and infiltrating CCR2 + monocytes to laser-induced CNV in mice. Five days after intravitreal injection of WORT, laser photocoagulation was induced in mice. On day 7 post laser induction, RPE/choroidal whole-mounts were processed and stained with IB4 and anti-Iba1 for assessment of neovascular membrane and macrophage accumulation, respectively. ( A ) Representative images show 3D reconstructions of CNV lesions captured by confocal microscopy and analyzed by Volocity Imaging software. Quantitative analysis shows that the WORT-induced RPE pathology exaggerates CNV volume ( B ) and lesional macrophages ( C ) compared to control. ( D ) Flow cytometric analysis confirms significant depletion of peripheral Ly6C hi CCR2 + monocytes by ip. injection of anti-CCR2 mAb (MC-21), compared with the rat IgG2b isotype. ( E ) MFI analysis using ImageJ software demonstrates reduced macrophage accumulation at CNV lesions by MC-21 injection. ( F ) Comparison of CNV volume shows abolishment of WORT induced laser-CNV by depletion of CCR2 + monocytes. ( B,C,E,F ) n ≥ 24. ( D ) n = 8. * P
    Figure Legend Snippet: Contribution of RPE degeneration and infiltrating CCR2 + monocytes to laser-induced CNV in mice. Five days after intravitreal injection of WORT, laser photocoagulation was induced in mice. On day 7 post laser induction, RPE/choroidal whole-mounts were processed and stained with IB4 and anti-Iba1 for assessment of neovascular membrane and macrophage accumulation, respectively. ( A ) Representative images show 3D reconstructions of CNV lesions captured by confocal microscopy and analyzed by Volocity Imaging software. Quantitative analysis shows that the WORT-induced RPE pathology exaggerates CNV volume ( B ) and lesional macrophages ( C ) compared to control. ( D ) Flow cytometric analysis confirms significant depletion of peripheral Ly6C hi CCR2 + monocytes by ip. injection of anti-CCR2 mAb (MC-21), compared with the rat IgG2b isotype. ( E ) MFI analysis using ImageJ software demonstrates reduced macrophage accumulation at CNV lesions by MC-21 injection. ( F ) Comparison of CNV volume shows abolishment of WORT induced laser-CNV by depletion of CCR2 + monocytes. ( B,C,E,F ) n ≥ 24. ( D ) n = 8. * P

    Techniques Used: Mouse Assay, Injection, Staining, Confocal Microscopy, Imaging, Software, Flow Cytometry

    Related Articles

    Magnetic Beads:

    Article Title: Mycobacterium paratuberculosis detection in cow's milk in Argentina by immunomagnetic separation-PCR
    Article Snippet: .. Coating of immunomagnetic beads Goat anti-mouse IgG magnetic beads (New England BioLabs Inc., Ipswich, MA, USA) were mixed for 1 h at 4 °C under constant shaking. ..

    Incubation:

    Article Title: Identification of C/EBPα as a novel target of the HPV8 E6 protein regulating miR-203 in human keratinocytes
    Article Snippet: .. The cleared supernatant was incubated for 1 h at 4˚C with 3 μg of p300 monoclonal RW128 mouse antibody (Millipore) or mouse IgG1 (New England BioLabs). .. The immune complexes were precipitated with 60 μl protein A/G Sepharose (Santa Cruz Biotechnology) in the presence of salmon sperm DNA overnight at 4˚C.

    Article Title: Human cytomegalovirus uracil DNA glycosylase associates with ppUL44 and accelerates the accumulation of viral DNA
    Article Snippet: .. Blots were washed three times with washing buffer, and incubated at room temperature for 1 h with anti-Mouse IgG(H+L) HRP conjugate (New England Biolabs) diluted 1:2500 in washing buffer. ..

    other:

    Article Title: Impairing autophagy in retinal pigment epithelium leads to inflammasome activation and enhanced macrophage-mediated angiogenesis
    Article Snippet: Rabbit anti-cleaved caspase-3 mAb (5A1E), HRP conjugated goat anti-rabbit and anti-mouse IgG were from New England Biolabs (Hitchin, UK).

    Expressing:

    Article Title: An ancestral non-proteolytic role for presenilin proteins in multicellular development of the social amoeba Dictyostelium discoideum
    Article Snippet: .. For immunofluorescence labelling, cell lines expressing the D. discoideum or human genes were grown overnight in low-fluorescence medium, fixed, probed with anti-calnexin antibodies and labelled with goat anti-mouse-IgG (NEB 4409s; Alexa Fluor 555; Ipswich, MA). .. Rabbit monoclonal anti-presenilin-1 D39D1 antibody (#5643) was obtained from Cell Signaling Technology (Danvers, MA) and anti-presenilin-2 (EP1515Y) antibody (ab51249) was obtained from Abcam (Cambridge, MA) and used at 1∶1000 for western analysis.

    Western Blot:

    Article Title: Interaction of Bestrophin-1 and Ca2+ Channel ?-Subunits: Identification of New Binding Domains on the Bestrophin-1 C-Terminus
    Article Snippet: .. Proteins were visualized by Western blot using HRP-conjugated goat anti-rabbit or goat anti-mouse antibodies (New England Biolabs). .. Immunoprecipitation Subconfluent (70-80%) culture of CHO, HEK-293 or COS-7 cells were transiently transfected with combinations of plasmids encoding a human-bestrophin-1 (N-terminal EGFP-tagged or untagged), β3 (untagged), β4-His6 and CaV 1.3 (N-terminal eGFP-tagged or untagged) subunits of voltage-dependent calcium channels using Lipofectamine 2000 transfection reagent (Invitrogen) according to the manufacturer's protocol.

    Immunofluorescence:

    Article Title: An ancestral non-proteolytic role for presenilin proteins in multicellular development of the social amoeba Dictyostelium discoideum
    Article Snippet: .. For immunofluorescence labelling, cell lines expressing the D. discoideum or human genes were grown overnight in low-fluorescence medium, fixed, probed with anti-calnexin antibodies and labelled with goat anti-mouse-IgG (NEB 4409s; Alexa Fluor 555; Ipswich, MA). .. Rabbit monoclonal anti-presenilin-1 D39D1 antibody (#5643) was obtained from Cell Signaling Technology (Danvers, MA) and anti-presenilin-2 (EP1515Y) antibody (ab51249) was obtained from Abcam (Cambridge, MA) and used at 1∶1000 for western analysis.

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  • 94
    New England Biolabs anti mouse igg
    Contribution of RPE degeneration and infiltrating CCR2 + monocytes to laser-induced CNV in mice. Five days after intravitreal injection of WORT, laser photocoagulation was induced in mice. On day 7 post laser induction, RPE/choroidal whole-mounts were processed and stained with IB4 and anti-Iba1 for assessment of neovascular membrane and macrophage accumulation, respectively. ( A ) Representative images show 3D reconstructions of CNV lesions captured by confocal microscopy and analyzed by Volocity Imaging software. Quantitative analysis shows that the WORT-induced RPE pathology exaggerates CNV volume ( B ) and lesional macrophages ( C ) compared to control. ( D ) Flow cytometric analysis confirms significant depletion of peripheral Ly6C hi CCR2 + monocytes by ip. injection of anti-CCR2 <t>mAb</t> (MC-21), compared with the rat <t>IgG2b</t> isotype. ( E ) MFI analysis using ImageJ software demonstrates reduced macrophage accumulation at CNV lesions by MC-21 injection. ( F ) Comparison of CNV volume shows abolishment of WORT induced laser-CNV by depletion of CCR2 + monocytes. ( B,C,E,F ) n ≥ 24. ( D ) n = 8. * P
    Anti Mouse Igg, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse igg/product/New England Biolabs
    Average 94 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    anti mouse igg - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    Image Search Results


    Contribution of RPE degeneration and infiltrating CCR2 + monocytes to laser-induced CNV in mice. Five days after intravitreal injection of WORT, laser photocoagulation was induced in mice. On day 7 post laser induction, RPE/choroidal whole-mounts were processed and stained with IB4 and anti-Iba1 for assessment of neovascular membrane and macrophage accumulation, respectively. ( A ) Representative images show 3D reconstructions of CNV lesions captured by confocal microscopy and analyzed by Volocity Imaging software. Quantitative analysis shows that the WORT-induced RPE pathology exaggerates CNV volume ( B ) and lesional macrophages ( C ) compared to control. ( D ) Flow cytometric analysis confirms significant depletion of peripheral Ly6C hi CCR2 + monocytes by ip. injection of anti-CCR2 mAb (MC-21), compared with the rat IgG2b isotype. ( E ) MFI analysis using ImageJ software demonstrates reduced macrophage accumulation at CNV lesions by MC-21 injection. ( F ) Comparison of CNV volume shows abolishment of WORT induced laser-CNV by depletion of CCR2 + monocytes. ( B,C,E,F ) n ≥ 24. ( D ) n = 8. * P

    Journal: Scientific Reports

    Article Title: Impairing autophagy in retinal pigment epithelium leads to inflammasome activation and enhanced macrophage-mediated angiogenesis

    doi: 10.1038/srep20639

    Figure Lengend Snippet: Contribution of RPE degeneration and infiltrating CCR2 + monocytes to laser-induced CNV in mice. Five days after intravitreal injection of WORT, laser photocoagulation was induced in mice. On day 7 post laser induction, RPE/choroidal whole-mounts were processed and stained with IB4 and anti-Iba1 for assessment of neovascular membrane and macrophage accumulation, respectively. ( A ) Representative images show 3D reconstructions of CNV lesions captured by confocal microscopy and analyzed by Volocity Imaging software. Quantitative analysis shows that the WORT-induced RPE pathology exaggerates CNV volume ( B ) and lesional macrophages ( C ) compared to control. ( D ) Flow cytometric analysis confirms significant depletion of peripheral Ly6C hi CCR2 + monocytes by ip. injection of anti-CCR2 mAb (MC-21), compared with the rat IgG2b isotype. ( E ) MFI analysis using ImageJ software demonstrates reduced macrophage accumulation at CNV lesions by MC-21 injection. ( F ) Comparison of CNV volume shows abolishment of WORT induced laser-CNV by depletion of CCR2 + monocytes. ( B,C,E,F ) n ≥ 24. ( D ) n = 8. * P

    Article Snippet: Rabbit anti-cleaved caspase-3 mAb (5A1E), HRP conjugated goat anti-rabbit and anti-mouse IgG were from New England Biolabs (Hitchin, UK).

    Techniques: Mouse Assay, Injection, Staining, Confocal Microscopy, Imaging, Software, Flow Cytometry