mrna library preparation  (New England Biolabs)


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    Name:
    Oligo dT25 Magnetic Beads
    Description:
    Oligo dT25 Magnetic Beads 5 ml
    Catalog Number:
    s1419s
    Price:
    292
    Size:
    5 ml
    Category:
    RNA Purification Kit Components
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    Structured Review

    New England Biolabs mrna library preparation
    Oligo dT25 Magnetic Beads
    Oligo dT25 Magnetic Beads 5 ml
    https://www.bioz.com/result/mrna library preparation/product/New England Biolabs
    Average 90 stars, based on 203 article reviews
    Price from $9.99 to $1999.99
    mrna library preparation - by Bioz Stars, 2020-07
    90/100 stars

    Images

    1) Product Images from "Comprehensive Analysis of the Characteristics and Differences in Adult and Newborn Brown Adipose Tissue (BAT): Newborn BAT Is a More Active/Dynamic BAT"

    Article Title: Comprehensive Analysis of the Characteristics and Differences in Adult and Newborn Brown Adipose Tissue (BAT): Newborn BAT Is a More Active/Dynamic BAT

    Journal: Cells

    doi: 10.3390/cells9010201

    Competing endogenous RNA network ( A ) with mRNA, circRNA and DNA methylation regulation networks ( B , C ) in neBAT and aBAT. ( A ) The competing endogenous RNA network was based on miRNA, circRNA, and mRNA interactions. ( B ) The mRNA, circRNA, and DNA methylation regulation network was based on mRNA, circRNA, and DNA methylation interactions. ( C ) The regulation network of circRNA, lnc RNA, and mRNA in aBAT and neBAT.
    Figure Legend Snippet: Competing endogenous RNA network ( A ) with mRNA, circRNA and DNA methylation regulation networks ( B , C ) in neBAT and aBAT. ( A ) The competing endogenous RNA network was based on miRNA, circRNA, and mRNA interactions. ( B ) The mRNA, circRNA, and DNA methylation regulation network was based on mRNA, circRNA, and DNA methylation interactions. ( C ) The regulation network of circRNA, lnc RNA, and mRNA in aBAT and neBAT.

    Techniques Used: DNA Methylation Assay

    Related Articles

    Amplification:

    Article Title: DOT1L-Mediated H3K79 Methylation in Chromatin Is Dispensable for Wnt Pathway-Specific and Other Intestinal Epithelial Functions
    Article Snippet: .. For RNA sequencing (RNA-seq), mRNA was isolated from total RNA using oligo(dT)25 magnetic beads (New England BioLabs). cDNA was synthesized, sonicated, amplified using the Encore Complete RNA-Seq Library System (Nugen), and sequenced using Illumina Hi-seq 2000. .. Sequence tags were mapped to the mouse reference genome, build mm9, and differences in transcript levels were determined using Tophat and Cufflinks software ( ) at a false-discovery rate (FDR) of 0.001.

    Article Title: Ovaries absent links dLsd1 to HP1a for local H3K4 demethylation required for heterochromatic gene silencing
    Article Snippet: .. 10 μg of total RNA from each sample used for library preparation after poly(A)-containing mRNA molecule purification (NEB, #S1419S), RNA amplification, double-strand cDNA synthesis, and adaptor ligation (NEB, #E7645S). .. 10 μg of total RNA from each sample used for library preparation after poly(A)-containing mRNA molecule purification (NEB, #S1419S), RNA amplification, double-strand cDNA synthesis, and adaptor ligation (NEB, #E7645S).

    RNA Sequencing Assay:

    Article Title: DOT1L-Mediated H3K79 Methylation in Chromatin Is Dispensable for Wnt Pathway-Specific and Other Intestinal Epithelial Functions
    Article Snippet: .. For RNA sequencing (RNA-seq), mRNA was isolated from total RNA using oligo(dT)25 magnetic beads (New England BioLabs). cDNA was synthesized, sonicated, amplified using the Encore Complete RNA-Seq Library System (Nugen), and sequenced using Illumina Hi-seq 2000. .. Sequence tags were mapped to the mouse reference genome, build mm9, and differences in transcript levels were determined using Tophat and Cufflinks software ( ) at a false-discovery rate (FDR) of 0.001.

    Magnetic Beads:

    Article Title: Discovery of RNA-binding proteins and characterization of their dynamic responses by enhanced RNA interactome capture
    Article Snippet: .. Capture of RNP complexes using RIC Lysates were thawed in a 37 °C water bath, and after taking 100 μL as input, they were incubated with 300 μL of equilibrated oligo(dT)25 magnetic beads (NEB) at 4 °C for 1 h with gentle rotation. ..

    Article Title: DOT1L-Mediated H3K79 Methylation in Chromatin Is Dispensable for Wnt Pathway-Specific and Other Intestinal Epithelial Functions
    Article Snippet: .. For RNA sequencing (RNA-seq), mRNA was isolated from total RNA using oligo(dT)25 magnetic beads (New England BioLabs). cDNA was synthesized, sonicated, amplified using the Encore Complete RNA-Seq Library System (Nugen), and sequenced using Illumina Hi-seq 2000. .. Sequence tags were mapped to the mouse reference genome, build mm9, and differences in transcript levels were determined using Tophat and Cufflinks software ( ) at a false-discovery rate (FDR) of 0.001.

    Article Title: The RNA-binding protein repertoire of Arabidopsis thaliana
    Article Snippet: .. Following the capture poly(A)+ RNAs using Oligo(dT)25 magnetic beads, the eluted sample was split into three fractions. .. One fraction was directly concentrated using a freeze drier to concentrate RNA-RBP complexes, the second fraction was used for RBP isolation as described previously and the third fraction was used for RNA isolation as described previously .

    Article Title: The C9ORF72 Gene, Implicated in Amyotrophic Lateral Sclerosis and Frontotemporal Dementia, Encodes a Protein That Functions in Control of Endothelin and Glutamate Signaling
    Article Snippet: .. Total RNA was subjected to 2 rounds of poly(A) selection using oligo(dT)25 magnetic beads (New England BioLabs). .. Single-read cDNA libraries were prepared following the Illumina TrueSeq small RNA protocol for strand-specific RNA-seq , with minor modifications.

    Article Title: Evolution of the RNA N6-Methyladenosine Methylome Mediated by Genomic Duplication
    Article Snippet: .. Polyadenylated (PolyA+) mRNA selection was performed using oligo(dT)25 magnetic beads (code no. S1419S; New England Biolabs) following the manufacturer’s protocol. .. mRNA was randomly fragmented into ∼200-nucleotide-long fragments by RNA Fragmentation Reagents (Ambion).

    Isolation:

    Article Title: DOT1L-Mediated H3K79 Methylation in Chromatin Is Dispensable for Wnt Pathway-Specific and Other Intestinal Epithelial Functions
    Article Snippet: .. For RNA sequencing (RNA-seq), mRNA was isolated from total RNA using oligo(dT)25 magnetic beads (New England BioLabs). cDNA was synthesized, sonicated, amplified using the Encore Complete RNA-Seq Library System (Nugen), and sequenced using Illumina Hi-seq 2000. .. Sequence tags were mapped to the mouse reference genome, build mm9, and differences in transcript levels were determined using Tophat and Cufflinks software ( ) at a false-discovery rate (FDR) of 0.001.

    Article Title: scFTD-seq: freeze-thaw lysis based, portable approach toward highly distributed single-cell 3′ mRNA profiling
    Article Snippet: .. Following lysis, mRNA from lysates were isolated using magnetic oligo-dt beads (NEB, S1419S) following manufacturer's instructions. mRNA was quantified using NanoDrop 2000 spectrophotometer. .. For qPCR, 10 ng of mRNA was used as input for all conditions and reactions were run on CFX Connect Real-Time PCR machine (Biorad) using SsoFast EvaGreen PCR supermix (Biorad) following manufacturer's instructions.

    Ligation:

    Article Title: Ovaries absent links dLsd1 to HP1a for local H3K4 demethylation required for heterochromatic gene silencing
    Article Snippet: .. 10 μg of total RNA from each sample used for library preparation after poly(A)-containing mRNA molecule purification (NEB, #S1419S), RNA amplification, double-strand cDNA synthesis, and adaptor ligation (NEB, #E7645S). .. 10 μg of total RNA from each sample used for library preparation after poly(A)-containing mRNA molecule purification (NEB, #S1419S), RNA amplification, double-strand cDNA synthesis, and adaptor ligation (NEB, #E7645S).

    Spectrophotometry:

    Article Title: scFTD-seq: freeze-thaw lysis based, portable approach toward highly distributed single-cell 3′ mRNA profiling
    Article Snippet: .. Following lysis, mRNA from lysates were isolated using magnetic oligo-dt beads (NEB, S1419S) following manufacturer's instructions. mRNA was quantified using NanoDrop 2000 spectrophotometer. .. For qPCR, 10 ng of mRNA was used as input for all conditions and reactions were run on CFX Connect Real-Time PCR machine (Biorad) using SsoFast EvaGreen PCR supermix (Biorad) following manufacturer's instructions.

    Purification:

    Article Title: Ovaries absent links dLsd1 to HP1a for local H3K4 demethylation required for heterochromatic gene silencing
    Article Snippet: .. 10 μg of total RNA from each sample used for library preparation after poly(A)-containing mRNA molecule purification (NEB, #S1419S), RNA amplification, double-strand cDNA synthesis, and adaptor ligation (NEB, #E7645S). .. 10 μg of total RNA from each sample used for library preparation after poly(A)-containing mRNA molecule purification (NEB, #S1419S), RNA amplification, double-strand cDNA synthesis, and adaptor ligation (NEB, #E7645S).

    Synthesized:

    Article Title: DOT1L-Mediated H3K79 Methylation in Chromatin Is Dispensable for Wnt Pathway-Specific and Other Intestinal Epithelial Functions
    Article Snippet: .. For RNA sequencing (RNA-seq), mRNA was isolated from total RNA using oligo(dT)25 magnetic beads (New England BioLabs). cDNA was synthesized, sonicated, amplified using the Encore Complete RNA-Seq Library System (Nugen), and sequenced using Illumina Hi-seq 2000. .. Sequence tags were mapped to the mouse reference genome, build mm9, and differences in transcript levels were determined using Tophat and Cufflinks software ( ) at a false-discovery rate (FDR) of 0.001.

    Incubation:

    Article Title: Discovery of RNA-binding proteins and characterization of their dynamic responses by enhanced RNA interactome capture
    Article Snippet: .. Capture of RNP complexes using RIC Lysates were thawed in a 37 °C water bath, and after taking 100 μL as input, they were incubated with 300 μL of equilibrated oligo(dT)25 magnetic beads (NEB) at 4 °C for 1 h with gentle rotation. ..

    Selection:

    Article Title: The C9ORF72 Gene, Implicated in Amyotrophic Lateral Sclerosis and Frontotemporal Dementia, Encodes a Protein That Functions in Control of Endothelin and Glutamate Signaling
    Article Snippet: .. Total RNA was subjected to 2 rounds of poly(A) selection using oligo(dT)25 magnetic beads (New England BioLabs). .. Single-read cDNA libraries were prepared following the Illumina TrueSeq small RNA protocol for strand-specific RNA-seq , with minor modifications.

    Article Title: Evolution of the RNA N6-Methyladenosine Methylome Mediated by Genomic Duplication
    Article Snippet: .. Polyadenylated (PolyA+) mRNA selection was performed using oligo(dT)25 magnetic beads (code no. S1419S; New England Biolabs) following the manufacturer’s protocol. .. mRNA was randomly fragmented into ∼200-nucleotide-long fragments by RNA Fragmentation Reagents (Ambion).

    Sonication:

    Article Title: DOT1L-Mediated H3K79 Methylation in Chromatin Is Dispensable for Wnt Pathway-Specific and Other Intestinal Epithelial Functions
    Article Snippet: .. For RNA sequencing (RNA-seq), mRNA was isolated from total RNA using oligo(dT)25 magnetic beads (New England BioLabs). cDNA was synthesized, sonicated, amplified using the Encore Complete RNA-Seq Library System (Nugen), and sequenced using Illumina Hi-seq 2000. .. Sequence tags were mapped to the mouse reference genome, build mm9, and differences in transcript levels were determined using Tophat and Cufflinks software ( ) at a false-discovery rate (FDR) of 0.001.

    Lysis:

    Article Title: scFTD-seq: freeze-thaw lysis based, portable approach toward highly distributed single-cell 3′ mRNA profiling
    Article Snippet: .. Following lysis, mRNA from lysates were isolated using magnetic oligo-dt beads (NEB, S1419S) following manufacturer's instructions. mRNA was quantified using NanoDrop 2000 spectrophotometer. .. For qPCR, 10 ng of mRNA was used as input for all conditions and reactions were run on CFX Connect Real-Time PCR machine (Biorad) using SsoFast EvaGreen PCR supermix (Biorad) following manufacturer's instructions.

    Article Title: Capturing the interactome of newly transcribed RNA
    Article Snippet: .. 100 μl oligo(dT) beads (New England Biolabs, cat# S1419) were washed twice using lysis buffer and added to the cell lysate to incubate for 1 h at 4 °C. ..

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  • 99
    New England Biolabs oligo dt25 magnetic beads
    Oligo Dt25 Magnetic Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo dt25 magnetic beads/product/New England Biolabs
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    oligo dt25 magnetic beads - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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