s1411  (New England Biolabs)


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    Name:
    3 O Me m7G 5 ppp 5 G RNA Cap Structure Analog
    Description:
    3 O Me m7G 5 ppp 5 G RNA Cap Structure Analog 5 umol
    Catalog Number:
    s1411l
    Price:
    604
    Size:
    5 umol
    Category:
    Capping Reagents for DNA RNA Synthesis
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    Structured Review

    New England Biolabs s1411
    3 O Me m7G 5 ppp 5 G RNA Cap Structure Analog
    3 O Me m7G 5 ppp 5 G RNA Cap Structure Analog 5 umol
    https://www.bioz.com/result/s1411/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    s1411 - by Bioz Stars, 2020-09
    97/100 stars

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    Related Articles

    Modification:

    Article Title: Dom34 mediates targeting of exogenous RNA in the antiviral OAS/RNase L pathway
    Article Snippet: .. For the synthesis of mRNA whose 5′-terminus was modified with a cap structure, 3′-O-Me-m7G(5′)ppp(5′)G cap analog (New England Biolabs) was added during the T7 polymerase reaction. .. Following DNase I treatment, the synthetic RNAs were purified using a illustra MicroSpin G-25 column (GE Healthcare) or illustra MicroSpin S-400 HR column (GE Healthcare) and quantified using NanoDrop One (Thermo Fisher Scientific).

    Article Title: Lipid-mRNA Nanoparticle Designed to Enhance Intracellular Delivery Mediated by Shock Waves
    Article Snippet: .. The mMESSAGE mMACHINE Kit (Invitrogen AM1344) was used for in vitro transcription using native NTP s. The MEGAscript T7 Transcription Kit (Invitrogen AM1333) was used for mRNA synthesis using ARCA (NEB S1411S) and pseudo-UTP (Jena Bioscience NU-1139S) with a modified protocol. .. The reaction (1 μg of PCR product, 7.5 mM of ATP, CTP, and pseudo-UTP, 1.5 mM of GTP, 6 mM of ARCA, 2 μL of 10× reaction buffer and 2 μL of enzyme mix) was diluted to 20 μL of the final volume with nuclease-free water and incubated at 37 °C for 4 h followed by TURBO DNase (final concentration of 2 U/μL) treatment at 37 °C for 15 min. A poly(A) tail was added to the synthesized mRNA using a poly(A) Tailing Kit (Invitrogen AM1350).

    Generated:

    Article Title: Incorporation of pseudouridine into mRNA enhances translation by diminishing PKR activation
    Article Snippet: .. Except where otherwise specified, capped mRNA was generated by performing transcription in the presence of cap analog 3′-O-Me-m7G(5′)ppp(5′)G (New England Biolabs). ..

    In Vitro:

    Article Title: An IGF1R-dependent pathway drives epicardial adipose tissue formation after myocardial injury
    Article Snippet: .. ModRNAs were in vitro transcribed from plasmid templates (sequences provided in the ) using a custom ribonucleoside blend of 3′-O-Me-m7G(5′)ppp(5′)G cap analog (6 mM, New England Biolabs), guanosine triphosphate (1.5 mM, USB), adenosine triphosphate (7.5 mM, USB), and 5-methylcytidine triphosphate and pseudouridine triphosphate (7.5 mM, TriLink Biotechnologies)as described previously , , RNA was treated with Antarctic Phosphatase (New England Biolabs), quantitated by Nanodrop (Thermo Scientific), precipitated with ethanol and Ammonium Acetate, and resuspended in 10 mM TrisHCl, 1 mM EDTA. .. ModRNA was transfected into cultured cells using RNAiMAX (Life Technologies).

    Article Title: Lipid-mRNA Nanoparticle Designed to Enhance Intracellular Delivery Mediated by Shock Waves
    Article Snippet: .. The mMESSAGE mMACHINE Kit (Invitrogen AM1344) was used for in vitro transcription using native NTP s. The MEGAscript T7 Transcription Kit (Invitrogen AM1333) was used for mRNA synthesis using ARCA (NEB S1411S) and pseudo-UTP (Jena Bioscience NU-1139S) with a modified protocol. .. The reaction (1 μg of PCR product, 7.5 mM of ATP, CTP, and pseudo-UTP, 1.5 mM of GTP, 6 mM of ARCA, 2 μL of 10× reaction buffer and 2 μL of enzyme mix) was diluted to 20 μL of the final volume with nuclease-free water and incubated at 37 °C for 4 h followed by TURBO DNase (final concentration of 2 U/μL) treatment at 37 °C for 15 min. A poly(A) tail was added to the synthesized mRNA using a poly(A) Tailing Kit (Invitrogen AM1350).

    Article Title: Identification and characterization of hippuristanol-resistant mutants reveals eIF4A1 dependencies within mRNA 5' leader regions.
    Article Snippet: .. In vitro transcription Reporters were linearized with BamHI, purified using the EZ-10 spin column DNA Cleanup Miniprep Kit (BioBasic, BS367) and 3 g was transcribed with T3 RNA polymerase (NEB, M0378), 10× RNA polymerase buffer (NEB), 0.5 mM CTP, 0.5 mM ATP, 0.5 mM UTP, 0.1 mM GTP, 0.5 mM 3′-O-Me-m7GpppG (Anti-Reverse Cap Analog [ARCA]; NEB, S1411), 100 U RNase Inhibitor (NEB, M0307) in a volume of 100 l for 3 h at 37◦C. .. DNaseI (5U, NEB, M0303) was then added, and samples were incubated for an additional 30 min at 37◦C.

    Plasmid Preparation:

    Article Title: An IGF1R-dependent pathway drives epicardial adipose tissue formation after myocardial injury
    Article Snippet: .. ModRNAs were in vitro transcribed from plasmid templates (sequences provided in the ) using a custom ribonucleoside blend of 3′-O-Me-m7G(5′)ppp(5′)G cap analog (6 mM, New England Biolabs), guanosine triphosphate (1.5 mM, USB), adenosine triphosphate (7.5 mM, USB), and 5-methylcytidine triphosphate and pseudouridine triphosphate (7.5 mM, TriLink Biotechnologies)as described previously , , RNA was treated with Antarctic Phosphatase (New England Biolabs), quantitated by Nanodrop (Thermo Scientific), precipitated with ethanol and Ammonium Acetate, and resuspended in 10 mM TrisHCl, 1 mM EDTA. .. ModRNA was transfected into cultured cells using RNAiMAX (Life Technologies).

    Purification:

    Article Title: Identification and characterization of hippuristanol-resistant mutants reveals eIF4A1 dependencies within mRNA 5' leader regions.
    Article Snippet: .. In vitro transcription Reporters were linearized with BamHI, purified using the EZ-10 spin column DNA Cleanup Miniprep Kit (BioBasic, BS367) and 3 g was transcribed with T3 RNA polymerase (NEB, M0378), 10× RNA polymerase buffer (NEB), 0.5 mM CTP, 0.5 mM ATP, 0.5 mM UTP, 0.1 mM GTP, 0.5 mM 3′-O-Me-m7GpppG (Anti-Reverse Cap Analog [ARCA]; NEB, S1411), 100 U RNase Inhibitor (NEB, M0307) in a volume of 100 l for 3 h at 37◦C. .. DNaseI (5U, NEB, M0303) was then added, and samples were incubated for an additional 30 min at 37◦C.

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  • 97
    New England Biolabs 3 o me m7g 5 ppp 5 g rna cap structure analog
    3 O Me M7g 5 Ppp 5 G Rna Cap Structure Analog, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 o me m7g 5 ppp 5 g rna cap structure analog/product/New England Biolabs
    Average 97 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    3 o me m7g 5 ppp 5 g rna cap structure analog - by Bioz Stars, 2020-09
    97/100 stars
      Buy from Supplier

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