oligo  (New England Biolabs)


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  • 94
    Name:
    Oligo dT 25 Cellulose
    Description:
    Oligo dT 25 Cellulose 250 mg
    Catalog Number:
    s1408s
    Price:
    136
    Size:
    250 mg
    Category:
    RNA Purification Kit Components
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    Structured Review

    New England Biolabs oligo
    Oligo dT 25 Cellulose
    Oligo dT 25 Cellulose 250 mg
    https://www.bioz.com/result/oligo/product/New England Biolabs
    Average 94 stars, based on 90 article reviews
    Price from $9.99 to $1999.99
    oligo - by Bioz Stars, 2020-07
    94/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Myosin VI regulates gene pairing and transcriptional pause release in T cells
    Article Snippet: .. For the nascent RNA qPCR analysis, mature poly(A)+ RNA was removed using oligo (dT) 25-cellulose beads (New England Biolabs). .. The PCR assay was performed in duplicate on a 7500 ABI Real-Time PCR System (Applied Biosystems) using TaqMan probes (Applied Biosystems) or SYBR Green.

    Isolation:

    Article Title: Pseudouridine profiling reveals regulated mRNA pseudouridylation in yeast and human cells
    Article Snippet: .. PolyA+ RNA was isolated from 10 mg (yeast) or 2 mg (HeLa) total RNA using oligo dT cellulose beads (NEB; S1408S), as described . .. For some libraries, two sequential rounds of polyA selection were performed.

    Article Title: Novel human ABCC9/SUR2 brain-expressed transcripts and an eQTL relevant to hippocampal sclerosis of aging
    Article Snippet: .. Poly-A mRNA isolation used an affinity oligo dT matrix (Oligo (dT)25 cellulose beads; New England Biolabs) isolation method following manufacturer’s instructions with minor modifications. .. Briefly, 0.5 –1 mg of total RNA isolated from hippocampus or skeletal muscle was dissolved in 0.5 mL loading buffer (0.5 M NaCl, 20 mM Tris-HCl, pH 7.5, 1 mM EDTA) and heated to 65 °C for 5 min. RNA samples were quickly cooled down in ice for 5 min before incubated with 12.5 mg of Oligo (dT)25 cellulose beads.

    Purification:

    Article Title: Transcriptional Interference by Independently Regulated Genes Occurs in Any Relative Arrangement of the Genes and Is Influenced by Chromosomal Integration Position
    Article Snippet: .. Poly(A) RNA was purified from approximately 100 μg of total RNA with an affinity matrix consisting of oligo(dT)25 cellulose beads (New England BioLabs, Beverly, Mass.). .. Five hundred nanograms of poly(A) RNA was subject to One-Step reverse transcription-PCR (RT-PCR) as directed by manufacturer (Roche Molecular Biologicals, Mannheim, Germany) by utilizing 300 nM oligo1 and oligo2 (described above under “Orientation analyses”), 400 nM each deoxynucleoside triphosphate (dNTP), and 1.0 μCi of [32 P]dCTP, at 62°C for 30 min for RT (0°C for the no-RT step), and cycling at 94°C for 30 s, 62°C for 30 s, and 71°C for 1 min (25 repetitions).

    Chromatography:

    Article Title: Differential Hepatic Gene Expression Profile of Male Fathead Minnows Exposed to Daily Varying Dose of Environmental Contaminants Individually and in Mixture
    Article Snippet: .. mRNA from 1 to 2 mg of pooled total RNA was extracted using oligo(dT) 25-cellulose beads (NEB, Massachusette, United States) in a Poly-Prep® Chromatography Column (BioRad, CA, United States). .. Isolated mRNA was quantified using a NanoDropTM 1000 Spectrophotometer (Thermo Scientific, Delaware, United States).

    Article Title: Differential Hepatic Gene Expression Profile of Male Fathead Minnows Exposed to Daily Varying Dose of Environmental Contaminants Individually and in Mixture
    Article Snippet: .. Microarray Protocol mRNA from 1 to 2 mg of pooled total RNA was extracted using oligo(dT) 25-cellulose beads (NEB, Massachusette, United States) in a Poly-Prep® Chromatography Column (BioRad, CA, United States). .. Isolated mRNA was quantified using a NanoDropTM 1000 Spectrophotometer (Thermo Scientific, Delaware, United States).

    Microarray:

    Article Title: Differential Hepatic Gene Expression Profile of Male Fathead Minnows Exposed to Daily Varying Dose of Environmental Contaminants Individually and in Mixture
    Article Snippet: .. Microarray Protocol mRNA from 1 to 2 mg of pooled total RNA was extracted using oligo(dT) 25-cellulose beads (NEB, Massachusette, United States) in a Poly-Prep® Chromatography Column (BioRad, CA, United States). .. Isolated mRNA was quantified using a NanoDropTM 1000 Spectrophotometer (Thermo Scientific, Delaware, United States).

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  • Team
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  • Bioz Stars
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  • 94
    New England Biolabs oligo
    Oligo, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo/product/New England Biolabs
    Average 94 stars, based on 89 article reviews
    Price from $9.99 to $1999.99
    oligo - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

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