g 5 ppp 5 a rna cap structure analog  (New England Biolabs)


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    Name:
    G 5 ppp 5 A RNA Cap Structure Analog
    Description:
    G 5 ppp 5 A RNA Cap Structure Analog 5 umol
    Catalog Number:
    s1406l
    Price:
    551
    Size:
    5 umol
    Category:
    Capping Reagents for DNA RNA Synthesis
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    New England Biolabs g 5 ppp 5 a rna cap structure analog
    G 5 ppp 5 A RNA Cap Structure Analog
    G 5 ppp 5 A RNA Cap Structure Analog 5 umol
    https://www.bioz.com/result/g 5 ppp 5 a rna cap structure analog/product/New England Biolabs
    Average 86 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    g 5 ppp 5 a rna cap structure analog - by Bioz Stars, 2020-01
    86/100 stars

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    Related Articles

    Luciferase:

    Article Title: Role for the Phosphatidylinositol 3-Kinase-Akt-TOR Pathway during Sindbis Virus Replication in Arthropods
    Article Snippet: Firefly luciferase (FFluc)-encoding mRNA was produced from a pUC19-derived plasmid possessing a T7 promoter followed by a short nonviral 5′-UTR, the luciferase open reading frame (ORF), a short 3′-UTR, and a poly(A) sequence. .. Both templates were linearized with XhoI (New England BioLabs [NEB]) and transcribed in the presence of the RNA cap analog mGpppG (NEB) with T7 or Sp6 RNA polymerase (NEB), followed by DNase treatment for 15 min.

    Article Title: Requirement for the Amino-Terminal Domain of Sindbis Virus nsP4 during Virus Infection ▿
    Article Snippet: .. Templates were linearized with XhoI (New England Biolabs [NEB]) and transcribed in the presence of the RNA cap analog mGpppG (NEB) with Sp6 or T7 RNA polymerase (NEB) for SIN or luciferase, respectively. .. Transcription reactions were used directly for transfection into BHK-21 cells in serum-free DMEM using Lipofectamine 2000 (Invitrogen).

    Synthesized:

    Article Title: Non-canonical translation initiation of the spliced mRNA encoding the human T-cell leukemia virus type 1 basic leucine zipper protein
    Article Snippet: .. Bicistronic mRNAs with a nonfunctional Cap (Acap) were synthesized in the presence of 3 mM A(5′)ppp(5′)G cap-analog (#S1406S; New England Biolabs). .. In vitro translation In vitro translation reactions were carried out in nuclease-treated rabbit reticulocyte lysate (RRL; #L4960; Promega) as previously described ( ).

    Article Title: Mutagenic Analysis of the 3? cis-Acting Elements of the Rubella Virus Genome
    Article Snippet: The reaction contained reaction buffer (40 mM Tris-HCl [pH 7.5], 6 mM MgCl2 , 2 mM spermidine, 10 mM dithiothreitol [DTT]) (New England BioLabs), 1 mM ATP, CTP, GTP, and UTP (Pharmacia, Piscataway, N.J.), 2 mM RNA cap analog [m7 G(5′)pppG] (New England BioLabs), RNasin (1 U/μl; Boehringer Mannheim Biochemicals), and SP6 RNA polymerase (1 to 2 U/μl; Epicentre, Madison, Wis.). .. RNA transcripts for RNase probing or competition assays were synthesized by using T7 RNA polymerase (New England BioLabs) under the same conditions described above for SP6 transcription with no RNA cap analog added to the reaction (these plasmids contained the T7 promoter rather than the SP6 promoter used in Robo302 constructs).

    Construct:

    Article Title: Mutagenic Analysis of the 3? cis-Acting Elements of the Rubella Virus Genome
    Article Snippet: The reaction contained reaction buffer (40 mM Tris-HCl [pH 7.5], 6 mM MgCl2 , 2 mM spermidine, 10 mM dithiothreitol [DTT]) (New England BioLabs), 1 mM ATP, CTP, GTP, and UTP (Pharmacia, Piscataway, N.J.), 2 mM RNA cap analog [m7 G(5′)pppG] (New England BioLabs), RNasin (1 U/μl; Boehringer Mannheim Biochemicals), and SP6 RNA polymerase (1 to 2 U/μl; Epicentre, Madison, Wis.). .. RNA transcripts for RNase probing or competition assays were synthesized by using T7 RNA polymerase (New England BioLabs) under the same conditions described above for SP6 transcription with no RNA cap analog added to the reaction (these plasmids contained the T7 promoter rather than the SP6 promoter used in Robo302 constructs).

    Article Title: Requirement for the Amino-Terminal Domain of Sindbis Virus nsP4 during Virus Infection ▿
    Article Snippet: SIN- or luciferase-carrying constructs were purified with commercial spin columns (Zymo) and transcribed as described previously , with modification. .. Templates were linearized with XhoI (New England Biolabs [NEB]) and transcribed in the presence of the RNA cap analog mGpppG (NEB) with Sp6 or T7 RNA polymerase (NEB) for SIN or luciferase, respectively.

    Electrophoresis:

    Article Title: Secondary RNA structure and nucleotide specificity contribute to internal initiation mediated by the human tau 5? leader
    Article Snippet: Cap priming of messages with an A-cap was done by the addition of 3 µL 40mM G(5′)ppp(5′)A RNA cap structure analog (New England Biolabs Inc. cat# S1406L) and 2 µL 15 mM GTP to transcriptions. m7G capped RNAs were capped with ScriptCap™ m7G Capping system and all RNAs were tailed with the A-Plus™ Poly(A) Polymerase Tailing Kit (EPICENTRE Biotechnologies/CellScript, Inc. cat# SCCE0625, PAP5104H). .. RNA quality was verified by electrophoresis of 1 µg of RNA on a Reliant™ Precast RNA gel (LONZA cat# 54922) for 1.5 hrs and stained with SYBR® gold (Invitrogen cat# S11494).

    Article Title: Non-canonical translation initiation of the spliced mRNA encoding the human T-cell leukemia virus type 1 basic leucine zipper protein
    Article Snippet: RNA concentrations were determined spectrophotometrically using a Nanodrop 1000 (NanoDrop Technology, Wilmington, Delaware, USA), and RNA integrity was assessed by electrophoresis on denaturing formaldehyde agarose gel as detailed in ( ). .. Bicistronic mRNAs with a nonfunctional Cap (Acap) were synthesized in the presence of 3 mM A(5′)ppp(5′)G cap-analog (#S1406S; New England Biolabs).

    Incubation:

    Article Title: Characterization of Recombinant Dengue-2 Virus Derived from a Single Nucleotide Substitution in the 5? Noncoding Region
    Article Snippet: Transcription of Viral RNA and Derivation of Virus In vitro transcription of the XbaI-linearized infectious clone plasmids was performed for 2 h at 37°C by using the Ampliscribe T7 transcription kit (EpiCentre Technologies) and RNA cap structure analog 7 mG (5′) ppp (5′) A (New England Biolabs). .. The cells were shocked twice, then transferred to a 75 cm2 flask in Dulbecco's modified minimal essential medium (DMEM) containing 10% fetal bovine serum (FBS) supplemented with penicillin/streptomycin, and finally incubated in a 5% CO2 incubator at 37°C.

    Modification:

    Article Title: Requirement for the Amino-Terminal Domain of Sindbis Virus nsP4 during Virus Infection ▿
    Article Snippet: SIN- or luciferase-carrying constructs were purified with commercial spin columns (Zymo) and transcribed as described previously , with modification. .. Templates were linearized with XhoI (New England Biolabs [NEB]) and transcribed in the presence of the RNA cap analog mGpppG (NEB) with Sp6 or T7 RNA polymerase (NEB) for SIN or luciferase, respectively.

    Article Title: Characterization of Recombinant Dengue-2 Virus Derived from a Single Nucleotide Substitution in the 5? Noncoding Region
    Article Snippet: Transcription of Viral RNA and Derivation of Virus In vitro transcription of the XbaI-linearized infectious clone plasmids was performed for 2 h at 37°C by using the Ampliscribe T7 transcription kit (EpiCentre Technologies) and RNA cap structure analog 7 mG (5′) ppp (5′) A (New England Biolabs). .. The cells were shocked twice, then transferred to a 75 cm2 flask in Dulbecco's modified minimal essential medium (DMEM) containing 10% fetal bovine serum (FBS) supplemented with penicillin/streptomycin, and finally incubated in a 5% CO2 incubator at 37°C.

    Electroporation:

    Article Title: Characterization of Recombinant Dengue-2 Virus Derived from a Single Nucleotide Substitution in the 5? Noncoding Region
    Article Snippet: Transcription of Viral RNA and Derivation of Virus In vitro transcription of the XbaI-linearized infectious clone plasmids was performed for 2 h at 37°C by using the Ampliscribe T7 transcription kit (EpiCentre Technologies) and RNA cap structure analog 7 mG (5′) ppp (5′) A (New England Biolabs). .. The transcribed viral RNA was transfected into LLC-MK2 cells (0.5 mL containing 4–6 × 106 cells/mL) by electroporation (Bio-Rad Gene Pulser).

    Transfection:

    Article Title: Mutagenic Analysis of the 3? cis-Acting Elements of the Rubella Virus Genome
    Article Snippet: For transfection, 5′-capped RNA transcripts were runoff transcribed (transcription terminates at the end of the restricted template) from the wild-type (wt) Robo302 virus or one of the mutagenized derivatives that had been linearized with Eco RI. .. The reaction contained reaction buffer (40 mM Tris-HCl [pH 7.5], 6 mM MgCl2 , 2 mM spermidine, 10 mM dithiothreitol [DTT]) (New England BioLabs), 1 mM ATP, CTP, GTP, and UTP (Pharmacia, Piscataway, N.J.), 2 mM RNA cap analog [m7 G(5′)pppG] (New England BioLabs), RNasin (1 U/μl; Boehringer Mannheim Biochemicals), and SP6 RNA polymerase (1 to 2 U/μl; Epicentre, Madison, Wis.).

    Article Title: Requirement for the Amino-Terminal Domain of Sindbis Virus nsP4 during Virus Infection ▿
    Article Snippet: Paragraph title: In vitro RNA transcriptions and transfections. ... Templates were linearized with XhoI (New England Biolabs [NEB]) and transcribed in the presence of the RNA cap analog mGpppG (NEB) with Sp6 or T7 RNA polymerase (NEB) for SIN or luciferase, respectively.

    Article Title: Characterization of Recombinant Dengue-2 Virus Derived from a Single Nucleotide Substitution in the 5? Noncoding Region
    Article Snippet: Transcription of Viral RNA and Derivation of Virus In vitro transcription of the XbaI-linearized infectious clone plasmids was performed for 2 h at 37°C by using the Ampliscribe T7 transcription kit (EpiCentre Technologies) and RNA cap structure analog 7 mG (5′) ppp (5′) A (New England Biolabs). .. The transcribed viral RNA was transfected into LLC-MK2 cells (0.5 mL containing 4–6 × 106 cells/mL) by electroporation (Bio-Rad Gene Pulser).

    Generated:

    Article Title: Role for the Phosphatidylinositol 3-Kinase-Akt-TOR Pathway during Sindbis Virus Replication in Arthropods
    Article Snippet: The SINrep/GFP plasmid was generated by the removal of lacZ from the pSINrep5LacZ plasmid by XbaI/SphI digestion and replacement with a PCR product encoding GFP ( ). .. Both templates were linearized with XhoI (New England BioLabs [NEB]) and transcribed in the presence of the RNA cap analog mGpppG (NEB) with T7 or Sp6 RNA polymerase (NEB), followed by DNase treatment for 15 min.

    Polymerase Chain Reaction:

    Article Title: Role for the Phosphatidylinositol 3-Kinase-Akt-TOR Pathway during Sindbis Virus Replication in Arthropods
    Article Snippet: The SINrep/GFP plasmid was generated by the removal of lacZ from the pSINrep5LacZ plasmid by XbaI/SphI digestion and replacement with a PCR product encoding GFP ( ). .. Both templates were linearized with XhoI (New England BioLabs [NEB]) and transcribed in the presence of the RNA cap analog mGpppG (NEB) with T7 or Sp6 RNA polymerase (NEB), followed by DNase treatment for 15 min.

    Recombinant:

    Article Title: Mutagenic Analysis of the 3? cis-Acting Elements of the Rubella Virus Genome
    Article Snippet: Standard recombinant DNA techniques were used, with minor modifications. .. The reaction contained reaction buffer (40 mM Tris-HCl [pH 7.5], 6 mM MgCl2 , 2 mM spermidine, 10 mM dithiothreitol [DTT]) (New England BioLabs), 1 mM ATP, CTP, GTP, and UTP (Pharmacia, Piscataway, N.J.), 2 mM RNA cap analog [m7 G(5′)pppG] (New England BioLabs), RNasin (1 U/μl; Boehringer Mannheim Biochemicals), and SP6 RNA polymerase (1 to 2 U/μl; Epicentre, Madison, Wis.).

    Molecular Cloning:

    Article Title: Mutagenic Analysis of the 3? cis-Acting Elements of the Rubella Virus Genome
    Article Snippet: Paragraph title: Molecular cloning, in vitro transcription, and reverse transcription. ... The reaction contained reaction buffer (40 mM Tris-HCl [pH 7.5], 6 mM MgCl2 , 2 mM spermidine, 10 mM dithiothreitol [DTT]) (New England BioLabs), 1 mM ATP, CTP, GTP, and UTP (Pharmacia, Piscataway, N.J.), 2 mM RNA cap analog [m7 G(5′)pppG] (New England BioLabs), RNasin (1 U/μl; Boehringer Mannheim Biochemicals), and SP6 RNA polymerase (1 to 2 U/μl; Epicentre, Madison, Wis.).

    Plaque Assay:

    Article Title: Requirement for the Amino-Terminal Domain of Sindbis Virus nsP4 during Virus Infection ▿
    Article Snippet: Templates were linearized with XhoI (New England Biolabs [NEB]) and transcribed in the presence of the RNA cap analog mGpppG (NEB) with Sp6 or T7 RNA polymerase (NEB) for SIN or luciferase, respectively. .. For virus stock generation, medium was harvested after the cytopathic effect (CPE) was observed, at between 24 and 48 h. The titers of the recovered virus stocks were determined by plaque assay using BHK-21 cells.

    Purification:

    Article Title: Secondary RNA structure and nucleotide specificity contribute to internal initiation mediated by the human tau 5? leader
    Article Snippet: Plasmid DNA was linearized with either BamHI or NotI, and purified. .. Cap priming of messages with an A-cap was done by the addition of 3 µL 40mM G(5′)ppp(5′)A RNA cap structure analog (New England Biolabs Inc. cat# S1406L) and 2 µL 15 mM GTP to transcriptions. m7G capped RNAs were capped with ScriptCap™ m7G Capping system and all RNAs were tailed with the A-Plus™ Poly(A) Polymerase Tailing Kit (EPICENTRE Biotechnologies/CellScript, Inc. cat# SCCE0625, PAP5104H).

    Article Title: eIF4A alleviates the translational repression mediated by classical secondary structures more than by G-quadruplexes
    Article Snippet: This kit uses the anti-reverse cap analogue (ARCA) to ensure the cap is in the correct orientation. mRNA was purified using the MEGAclear kit (ThermoFisher AM1908) and quantified by nanodrop. .. For , 7.5 mM ATP/CTP/UTP, 1.5 mM GTP and either 6 mM ARCA (NEB S1411S) or 6 mM G(5′)ppp(5′)A RNA Cap Structure Analog (NEB S1406S) was used whereas for Figure , 7.5 mM ATP/CTP/UTP, 6 mM ARCA (S1411S) and either 1.5 mM GTP or 1.5 mM 7-deazaguanine (TriLink N-1044) was used.

    Article Title: Mutagenic Analysis of the 3? cis-Acting Elements of the Rubella Virus Genome
    Article Snippet: The reaction contained reaction buffer (40 mM Tris-HCl [pH 7.5], 6 mM MgCl2 , 2 mM spermidine, 10 mM dithiothreitol [DTT]) (New England BioLabs), 1 mM ATP, CTP, GTP, and UTP (Pharmacia, Piscataway, N.J.), 2 mM RNA cap analog [m7 G(5′)pppG] (New England BioLabs), RNasin (1 U/μl; Boehringer Mannheim Biochemicals), and SP6 RNA polymerase (1 to 2 U/μl; Epicentre, Madison, Wis.). .. The plasmids used in these reactions were prepared from large cultures and CsCl purified.

    Sequencing:

    Article Title: Role for the Phosphatidylinositol 3-Kinase-Akt-TOR Pathway during Sindbis Virus Replication in Arthropods
    Article Snippet: Firefly luciferase (FFluc)-encoding mRNA was produced from a pUC19-derived plasmid possessing a T7 promoter followed by a short nonviral 5′-UTR, the luciferase open reading frame (ORF), a short 3′-UTR, and a poly(A) sequence. .. Both templates were linearized with XhoI (New England BioLabs [NEB]) and transcribed in the presence of the RNA cap analog mGpppG (NEB) with T7 or Sp6 RNA polymerase (NEB), followed by DNase treatment for 15 min.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Secondary RNA structure and nucleotide specificity contribute to internal initiation mediated by the human tau 5? leader
    Article Snippet: MEGAscript® T7 kit (Applied Biosystems/Ambion catAM1334) was used for in-vitro transcription reactions. .. Cap priming of messages with an A-cap was done by the addition of 3 µL 40mM G(5′)ppp(5′)A RNA cap structure analog (New England Biolabs Inc. cat# S1406L) and 2 µL 15 mM GTP to transcriptions. m7G capped RNAs were capped with ScriptCap™ m7G Capping system and all RNAs were tailed with the A-Plus™ Poly(A) Polymerase Tailing Kit (EPICENTRE Biotechnologies/CellScript, Inc. cat# SCCE0625, PAP5104H).

    Plasmid Preparation:

    Article Title: Secondary RNA structure and nucleotide specificity contribute to internal initiation mediated by the human tau 5? leader
    Article Snippet: One µg of linearized plasmid DNA was transcribed at 37°C for 3hrs. .. Cap priming of messages with an A-cap was done by the addition of 3 µL 40mM G(5′)ppp(5′)A RNA cap structure analog (New England Biolabs Inc. cat# S1406L) and 2 µL 15 mM GTP to transcriptions. m7G capped RNAs were capped with ScriptCap™ m7G Capping system and all RNAs were tailed with the A-Plus™ Poly(A) Polymerase Tailing Kit (EPICENTRE Biotechnologies/CellScript, Inc. cat# SCCE0625, PAP5104H).

    Article Title: Mutagenic Analysis of the 3? cis-Acting Elements of the Rubella Virus Genome
    Article Snippet: The reaction contained reaction buffer (40 mM Tris-HCl [pH 7.5], 6 mM MgCl2 , 2 mM spermidine, 10 mM dithiothreitol [DTT]) (New England BioLabs), 1 mM ATP, CTP, GTP, and UTP (Pharmacia, Piscataway, N.J.), 2 mM RNA cap analog [m7 G(5′)pppG] (New England BioLabs), RNasin (1 U/μl; Boehringer Mannheim Biochemicals), and SP6 RNA polymerase (1 to 2 U/μl; Epicentre, Madison, Wis.). .. The plasmid DNA used as a template in these reactions was prepared by standard miniprep procedures.

    Article Title: Role for the Phosphatidylinositol 3-Kinase-Akt-TOR Pathway during Sindbis Virus Replication in Arthropods
    Article Snippet: The SINrep/GFP plasmid was generated by the removal of lacZ from the pSINrep5LacZ plasmid by XbaI/SphI digestion and replacement with a PCR product encoding GFP ( ). .. Both templates were linearized with XhoI (New England BioLabs [NEB]) and transcribed in the presence of the RNA cap analog mGpppG (NEB) with T7 or Sp6 RNA polymerase (NEB), followed by DNase treatment for 15 min.

    Article Title: Characterization of Recombinant Dengue-2 Virus Derived from a Single Nucleotide Substitution in the 5? Noncoding Region
    Article Snippet: Transcription of Viral RNA and Derivation of Virus In vitro transcription of the XbaI-linearized infectious clone plasmids was performed for 2 h at 37°C by using the Ampliscribe T7 transcription kit (EpiCentre Technologies) and RNA cap structure analog 7 mG (5′) ppp (5′) A (New England Biolabs). .. 200–500 ng plasmid DNA was used for each transcription reaction.

    Agarose Gel Electrophoresis:

    Article Title: eIF4A alleviates the translational repression mediated by classical secondary structures more than by G-quadruplexes
    Article Snippet: Integrity of RNA was checked by inspection on a native agarose gel. mRNAs used in and Figure were transcribed using the TranscriptAid T7 High Yield Transcription Kit (ThermoFisher K0441) as per manufacturer's instructions. .. For , 7.5 mM ATP/CTP/UTP, 1.5 mM GTP and either 6 mM ARCA (NEB S1411S) or 6 mM G(5′)ppp(5′)A RNA Cap Structure Analog (NEB S1406S) was used whereas for Figure , 7.5 mM ATP/CTP/UTP, 6 mM ARCA (S1411S) and either 1.5 mM GTP or 1.5 mM 7-deazaguanine (TriLink N-1044) was used.

    Article Title: Non-canonical translation initiation of the spliced mRNA encoding the human T-cell leukemia virus type 1 basic leucine zipper protein
    Article Snippet: RNA concentrations were determined spectrophotometrically using a Nanodrop 1000 (NanoDrop Technology, Wilmington, Delaware, USA), and RNA integrity was assessed by electrophoresis on denaturing formaldehyde agarose gel as detailed in ( ). .. Bicistronic mRNAs with a nonfunctional Cap (Acap) were synthesized in the presence of 3 mM A(5′)ppp(5′)G cap-analog (#S1406S; New England Biolabs).

    In Vitro:

    Article Title: Secondary RNA structure and nucleotide specificity contribute to internal initiation mediated by the human tau 5? leader
    Article Snippet: Paragraph title: In-vitro transcription ... Cap priming of messages with an A-cap was done by the addition of 3 µL 40mM G(5′)ppp(5′)A RNA cap structure analog (New England Biolabs Inc. cat# S1406L) and 2 µL 15 mM GTP to transcriptions. m7G capped RNAs were capped with ScriptCap™ m7G Capping system and all RNAs were tailed with the A-Plus™ Poly(A) Polymerase Tailing Kit (EPICENTRE Biotechnologies/CellScript, Inc. cat# SCCE0625, PAP5104H).

    Article Title: eIF4A alleviates the translational repression mediated by classical secondary structures more than by G-quadruplexes
    Article Snippet: Paragraph title: In vitro transcription ... For , 7.5 mM ATP/CTP/UTP, 1.5 mM GTP and either 6 mM ARCA (NEB S1411S) or 6 mM G(5′)ppp(5′)A RNA Cap Structure Analog (NEB S1406S) was used whereas for Figure , 7.5 mM ATP/CTP/UTP, 6 mM ARCA (S1411S) and either 1.5 mM GTP or 1.5 mM 7-deazaguanine (TriLink N-1044) was used.

    Article Title: Non-canonical translation initiation of the spliced mRNA encoding the human T-cell leukemia virus type 1 basic leucine zipper protein
    Article Snippet: Paragraph title: In vitro transcription ... Bicistronic mRNAs with a nonfunctional Cap (Acap) were synthesized in the presence of 3 mM A(5′)ppp(5′)G cap-analog (#S1406S; New England Biolabs).

    Article Title: Mutagenic Analysis of the 3? cis-Acting Elements of the Rubella Virus Genome
    Article Snippet: Paragraph title: Molecular cloning, in vitro transcription, and reverse transcription. ... The reaction contained reaction buffer (40 mM Tris-HCl [pH 7.5], 6 mM MgCl2 , 2 mM spermidine, 10 mM dithiothreitol [DTT]) (New England BioLabs), 1 mM ATP, CTP, GTP, and UTP (Pharmacia, Piscataway, N.J.), 2 mM RNA cap analog [m7 G(5′)pppG] (New England BioLabs), RNasin (1 U/μl; Boehringer Mannheim Biochemicals), and SP6 RNA polymerase (1 to 2 U/μl; Epicentre, Madison, Wis.).

    Article Title: Requirement for the Amino-Terminal Domain of Sindbis Virus nsP4 during Virus Infection ▿
    Article Snippet: Paragraph title: In vitro RNA transcriptions and transfections. ... Templates were linearized with XhoI (New England Biolabs [NEB]) and transcribed in the presence of the RNA cap analog mGpppG (NEB) with Sp6 or T7 RNA polymerase (NEB) for SIN or luciferase, respectively.

    Article Title: Characterization of Recombinant Dengue-2 Virus Derived from a Single Nucleotide Substitution in the 5? Noncoding Region
    Article Snippet: .. Transcription of Viral RNA and Derivation of Virus In vitro transcription of the XbaI-linearized infectious clone plasmids was performed for 2 h at 37°C by using the Ampliscribe T7 transcription kit (EpiCentre Technologies) and RNA cap structure analog 7 mG (5′) ppp (5′) A (New England Biolabs). .. 200–500 ng plasmid DNA was used for each transcription reaction.

    Produced:

    Article Title: Role for the Phosphatidylinositol 3-Kinase-Akt-TOR Pathway during Sindbis Virus Replication in Arthropods
    Article Snippet: Firefly luciferase (FFluc)-encoding mRNA was produced from a pUC19-derived plasmid possessing a T7 promoter followed by a short nonviral 5′-UTR, the luciferase open reading frame (ORF), a short 3′-UTR, and a poly(A) sequence. .. Both templates were linearized with XhoI (New England BioLabs [NEB]) and transcribed in the presence of the RNA cap analog mGpppG (NEB) with T7 or Sp6 RNA polymerase (NEB), followed by DNase treatment for 15 min.

    Staining:

    Article Title: Secondary RNA structure and nucleotide specificity contribute to internal initiation mediated by the human tau 5? leader
    Article Snippet: Cap priming of messages with an A-cap was done by the addition of 3 µL 40mM G(5′)ppp(5′)A RNA cap structure analog (New England Biolabs Inc. cat# S1406L) and 2 µL 15 mM GTP to transcriptions. m7G capped RNAs were capped with ScriptCap™ m7G Capping system and all RNAs were tailed with the A-Plus™ Poly(A) Polymerase Tailing Kit (EPICENTRE Biotechnologies/CellScript, Inc. cat# SCCE0625, PAP5104H). .. RNA quality was verified by electrophoresis of 1 µg of RNA on a Reliant™ Precast RNA gel (LONZA cat# 54922) for 1.5 hrs and stained with SYBR® gold (Invitrogen cat# S11494).

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    New England Biolabs g 5 ppp 5 a rna cap structure analog
    G 5 Ppp 5 A Rna Cap Structure Analog, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/g 5 ppp 5 a rna cap structure analog/product/New England Biolabs
    Average 86 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    g 5 ppp 5 a rna cap structure analog - by Bioz Stars, 2020-01
    86/100 stars
      Buy from Supplier

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