g 5 ppp 5 a rna cap structure analog  (New England Biolabs)


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    Name:
    G 5 ppp 5 A RNA Cap Structure Analog
    Description:
    G 5 ppp 5 A RNA Cap Structure Analog 5 umol
    Catalog Number:
    s1406l
    Price:
    562
    Size:
    5 umol
    Category:
    Capping Reagents for DNA RNA Synthesis
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    Structured Review

    New England Biolabs g 5 ppp 5 a rna cap structure analog
    G 5 ppp 5 A RNA Cap Structure Analog
    G 5 ppp 5 A RNA Cap Structure Analog 5 umol
    https://www.bioz.com/result/g 5 ppp 5 a rna cap structure analog/product/New England Biolabs
    Average 94 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    g 5 ppp 5 a rna cap structure analog - by Bioz Stars, 2020-07
    94/100 stars

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    Mutagenesis:

    Article Title: A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity
    Article Snippet: .. The WT and mutant infectious clone plasmids were linearized by XbaI digestion and in vitro transcribed using the T7 promoter (RiboMAX Large Scale RNA Production System, Promega) with the addition of a m7 G(5′)ppp(5′)A RNA cap structure analog (New England Biolabs). .. The in vitro transcribed RNA was purified using Micro Bio-Spin columns (Bio Rad) and transfected into Vero cells using Lipofectamine 2000.

    Incubation:

    Article Title: Toward the identification of viral cap-methyltransferase inhibitors by fluorescence screening assay
    Article Snippet: .. Reaction was started with 4.6 μl of a mix containing both GpppA (NEB ref S1406) and SAM at 8 μM and 2 μM final concentrations respectively and was incubated 20 min at 30 °C. .. For the detection of released SAH, the EPIgeneous™ methyltransferase kit was purchased from CisBio Bioassays and reconstituted according to the supplier protocols.

    Article Title: Toward the identification of viral cap-methyltransferase inhibitors by fluorescence screening assay.
    Article Snippet: .. Reaction was started with 4.6 ml of a mix containing both GpppA (NEB ref S1406) and SAM at 8 mM and 2 mM final concentrations respectively and was incubated 20 min at 30 C. For the detection of released SAH, the EPIgeneous™ methyltransferase kit was purchased from CisBio Bioassays and reconstituted according to the supplier protocols. .. HTRF assays were performed in white 384 Well Small Volume™ HiBase Polystyrene Microplates (Greiner) with a final volume of 20 ml.

    In Vitro:

    Article Title: A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity
    Article Snippet: .. The WT and mutant infectious clone plasmids were linearized by XbaI digestion and in vitro transcribed using the T7 promoter (RiboMAX Large Scale RNA Production System, Promega) with the addition of a m7 G(5′)ppp(5′)A RNA cap structure analog (New England Biolabs). .. The in vitro transcribed RNA was purified using Micro Bio-Spin columns (Bio Rad) and transfected into Vero cells using Lipofectamine 2000.

    Article Title: A Unique 5′ Translation Element Discovered in Triticum Mosaic Virus
    Article Snippet: .. All mRNAs, unless otherwise specified, were in vitro transcribed with either the 3′-0-Me-m7G(5′)ppp(5′)G or G(5′)ppp(5′)A cap analog (New England BioLabs) at 4 mM concentrations. ..

    Article Title: Characterization of Recombinant Dengue-2 Virus Derived from a Single Nucleotide Substitution in the 5? Noncoding Region
    Article Snippet: .. Transcription of Viral RNA and Derivation of Virus In vitro transcription of the XbaI-linearized infectious clone plasmids was performed for 2 h at 37°C by using the Ampliscribe T7 transcription kit (EpiCentre Technologies) and RNA cap structure analog 7 mG (5′) ppp (5′) A (New England Biolabs). .. 200–500 ng plasmid DNA was used for each transcription reaction.

    Synthesized:

    Article Title: Non-canonical translation initiation of the spliced mRNA encoding the human T-cell leukemia virus type 1 basic leucine zipper protein
    Article Snippet: .. Bicistronic mRNAs with a nonfunctional Cap (Acap) were synthesized in the presence of 3 mM A(5′)ppp(5′)G cap-analog (#S1406S; New England Biolabs). .. In vitro translation reactions were carried out in nuclease-treated rabbit reticulocyte lysate (RRL; #L4960; Promega) as previously described ( ).

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    New England Biolabs g 5 ppp 5 a rna cap structure analog
    G 5 Ppp 5 A Rna Cap Structure Analog, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/g 5 ppp 5 a rna cap structure analog/product/New England Biolabs
    Average 94 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    g 5 ppp 5 a rna cap structure analog - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

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