oligo d  (New England Biolabs)


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    Name:
    Oligo d T 23VN
    Description:
    Oligo d T 23VN 1 0 A260 units
    Catalog Number:
    s1327s
    Price:
    99
    Size:
    1 0 A260 units
    Category:
    Probes and Primers
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    Structured Review

    New England Biolabs oligo d
    Oligo d T 23VN
    Oligo d T 23VN 1 0 A260 units
    https://www.bioz.com/result/oligo d/product/New England Biolabs
    Average 94 stars, based on 106 article reviews
    Price from $9.99 to $1999.99
    oligo d - by Bioz Stars, 2020-07
    94/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Isolation and Live Imaging of Enteric Progenitors based on Sox10-Histone2BVenus Transgene Expression
    Article Snippet: .. To evaluate the presence of mRNAs for neural crest, stem cell, neuronal and glial genes in the limiting quantities of RNA obtained from flow-sorted cells, TaqMan Assay-On-Demand (AoD) gene expression assays were implemented using the PreAmp Master Mix System (ABI). cDNA was synthesized from 25 ng of total RNA by reverse transcription in a reaction containing dNTPs (Invitrogen), OligodT23VN (NEB), Random Hexamers (NEB), RT Buffer (ABI), RNasin (Promega), and MultiScribe Reverse Transcriptase (ABI). ..

    Agarose Gel Electrophoresis:

    Article Title: Genome-wide microarray analysis of tomato roots showed defined responses to iron deficiency
    Article Snippet: .. Real-time RT-PCR experiments 0.5 μg of total RNA (checked for quality and quantity using a spectrophotometer NanoDrop™ 1000 (Thermo Scientific), followed by a migration in an agarose gel) of each sample was retrotranscribed using 1 pmol of Oligo d(T)23VN (New England Biolabs, Beverly, USA) and 10 U M-MulV RNase H for 1 h at 42°C (Finnzymes, Helsinki, Finland) following the application protocol of the manufacturers. .. After RNA digestion with 1 U RNase A (USB, Cleveland, USA) for 1 h at 37°C, gene expression analyses were performed by adding 0.16 μl of the cDNA to the realtime PCR complete mix, FluoCycleTM sybr green (20 μl final volume; Euroclone, Pero, Italy), in a DNA Engine Opticon Real-Time PCR Detection (Biorad, Hercules, USA).

    Synthesized:

    Article Title: Isolation and Live Imaging of Enteric Progenitors based on Sox10-Histone2BVenus Transgene Expression
    Article Snippet: .. To evaluate the presence of mRNAs for neural crest, stem cell, neuronal and glial genes in the limiting quantities of RNA obtained from flow-sorted cells, TaqMan Assay-On-Demand (AoD) gene expression assays were implemented using the PreAmp Master Mix System (ABI). cDNA was synthesized from 25 ng of total RNA by reverse transcription in a reaction containing dNTPs (Invitrogen), OligodT23VN (NEB), Random Hexamers (NEB), RT Buffer (ABI), RNasin (Promega), and MultiScribe Reverse Transcriptase (ABI). ..

    Isolation:

    Article Title: Early transcriptomic response to Fe supply in Fe-deficient tomato plants is strongly influenced by the nature of the chelating agent
    Article Snippet: .. Real-time RT-PCR experiments Five hundreds nanograms of total RNA (isolated as previously described) of each sample was retrotranscribed using 1 pmol of Oligo d(T)23VN (New England Biolabs, Beverly, USA) and 10 U M-MulV RNase H- for 1 h at 42 °C (Finnzymes, Helsinki, Finland) following the application protocol of the manufacturers. .. After RNA digestion with 1 U RNase A (USB, Cleveland, USA) for 1 h at 37 °C, gene expression analyses were performed by adding 0.16 μL of the cDNA to the realtime PCR complete mix, FluoCycleTM sybr green (20 μL final volume; Euroclone, Pero, Italy), in a DNA Engine Opticon Real-Time PCR Detection (Biorad, Hercules, USA).

    Quantitative RT-PCR:

    Article Title: Genome-wide microarray analysis of tomato roots showed defined responses to iron deficiency
    Article Snippet: .. Real-time RT-PCR experiments 0.5 μg of total RNA (checked for quality and quantity using a spectrophotometer NanoDrop™ 1000 (Thermo Scientific), followed by a migration in an agarose gel) of each sample was retrotranscribed using 1 pmol of Oligo d(T)23VN (New England Biolabs, Beverly, USA) and 10 U M-MulV RNase H for 1 h at 42°C (Finnzymes, Helsinki, Finland) following the application protocol of the manufacturers. .. After RNA digestion with 1 U RNase A (USB, Cleveland, USA) for 1 h at 37°C, gene expression analyses were performed by adding 0.16 μl of the cDNA to the realtime PCR complete mix, FluoCycleTM sybr green (20 μl final volume; Euroclone, Pero, Italy), in a DNA Engine Opticon Real-Time PCR Detection (Biorad, Hercules, USA).

    Article Title: Early transcriptomic response to Fe supply in Fe-deficient tomato plants is strongly influenced by the nature of the chelating agent
    Article Snippet: .. Real-time RT-PCR experiments Five hundreds nanograms of total RNA (isolated as previously described) of each sample was retrotranscribed using 1 pmol of Oligo d(T)23VN (New England Biolabs, Beverly, USA) and 10 U M-MulV RNase H- for 1 h at 42 °C (Finnzymes, Helsinki, Finland) following the application protocol of the manufacturers. .. After RNA digestion with 1 U RNase A (USB, Cleveland, USA) for 1 h at 37 °C, gene expression analyses were performed by adding 0.16 μL of the cDNA to the realtime PCR complete mix, FluoCycleTM sybr green (20 μL final volume; Euroclone, Pero, Italy), in a DNA Engine Opticon Real-Time PCR Detection (Biorad, Hercules, USA).

    Article Title: Microfluidic platform combining droplets and magnetic tweezers: application to HER2 expression in cancer diagnosis
    Article Snippet: .. CellsDirect™ One-Step qRT-PCR kits (11732, Life Technologies) was used to prepare the Reverse transcription mix as follow: 12.5 μL of 2X Reaction Mix, 1 μL of anchored Oligo(dT)23VN (S1327S, NEB), 1 μL of each Forward and Reverse primers (custom primers to study HER2 or TBP or Actin b from IDT), 1 μL of SuperScript® III RT/Platinum® Taq Mix with RNaseOUT™ Ribonuclease Inhibitor and qsp to 25 μL with DEPC-treated water. ..

    Spectrophotometry:

    Article Title: Genome-wide microarray analysis of tomato roots showed defined responses to iron deficiency
    Article Snippet: .. Real-time RT-PCR experiments 0.5 μg of total RNA (checked for quality and quantity using a spectrophotometer NanoDrop™ 1000 (Thermo Scientific), followed by a migration in an agarose gel) of each sample was retrotranscribed using 1 pmol of Oligo d(T)23VN (New England Biolabs, Beverly, USA) and 10 U M-MulV RNase H for 1 h at 42°C (Finnzymes, Helsinki, Finland) following the application protocol of the manufacturers. .. After RNA digestion with 1 U RNase A (USB, Cleveland, USA) for 1 h at 37°C, gene expression analyses were performed by adding 0.16 μl of the cDNA to the realtime PCR complete mix, FluoCycleTM sybr green (20 μl final volume; Euroclone, Pero, Italy), in a DNA Engine Opticon Real-Time PCR Detection (Biorad, Hercules, USA).

    Expressing:

    Article Title: Isolation and Live Imaging of Enteric Progenitors based on Sox10-Histone2BVenus Transgene Expression
    Article Snippet: .. To evaluate the presence of mRNAs for neural crest, stem cell, neuronal and glial genes in the limiting quantities of RNA obtained from flow-sorted cells, TaqMan Assay-On-Demand (AoD) gene expression assays were implemented using the PreAmp Master Mix System (ABI). cDNA was synthesized from 25 ng of total RNA by reverse transcription in a reaction containing dNTPs (Invitrogen), OligodT23VN (NEB), Random Hexamers (NEB), RT Buffer (ABI), RNasin (Promega), and MultiScribe Reverse Transcriptase (ABI). ..

    Polymerase Chain Reaction:

    Article Title: Voltage-gated calcium channel subunit α2δ-3 shapes light responses of mouse retinal ganglion cells mainly in low and moderate light levels
    Article Snippet: .. In each reaction, 48-55 ng (adult mouse) or 300-450 ng (developmental time line) of RNA was used as template and retrotranscribed with oligo d(T) primers according to the manufacturer’s instructions. cDNA products were stored at −20°C and used as templates for polymerase chain reaction (PCR) using cDNA-specific primers for all α2 δ transcripts. ..

    Migration:

    Article Title: Genome-wide microarray analysis of tomato roots showed defined responses to iron deficiency
    Article Snippet: .. Real-time RT-PCR experiments 0.5 μg of total RNA (checked for quality and quantity using a spectrophotometer NanoDrop™ 1000 (Thermo Scientific), followed by a migration in an agarose gel) of each sample was retrotranscribed using 1 pmol of Oligo d(T)23VN (New England Biolabs, Beverly, USA) and 10 U M-MulV RNase H for 1 h at 42°C (Finnzymes, Helsinki, Finland) following the application protocol of the manufacturers. .. After RNA digestion with 1 U RNase A (USB, Cleveland, USA) for 1 h at 37°C, gene expression analyses were performed by adding 0.16 μl of the cDNA to the realtime PCR complete mix, FluoCycleTM sybr green (20 μl final volume; Euroclone, Pero, Italy), in a DNA Engine Opticon Real-Time PCR Detection (Biorad, Hercules, USA).

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  • Bioz Stars
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  • 94
    New England Biolabs oligo d
    Oligo D, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo d/product/New England Biolabs
    Average 94 stars, based on 103 article reviews
    Price from $9.99 to $1999.99
    oligo d - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

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