oligodeoxyribonucleotide microrna  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 80

    Structured Review

    New England Biolabs oligodeoxyribonucleotide microrna
    Oligodeoxyribonucleotide Microrna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligodeoxyribonucleotide microrna/product/New England Biolabs
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    oligodeoxyribonucleotide microrna - by Bioz Stars, 2020-01
    80/100 stars

    Related Products / Commonly Used Together

    t4 rna ligase 2
    noncoding rna

    Images

    Related Articles

    Clone Assay:

    Article Title:
    Article Snippet: .. A 5′-adenylated and 3′-blocked oligodeoxyribonucleotide microRNA (miRNA) cloning adaptor (5′ rApp CTG TAG GCA CCA TCA AT–NH2 3′; S1315; NEB) was ligated to the 3′ OH of the noncoding RNA using T4 RNA ligase 2 (M0351; NEB) by following the manufacturer's instructions. .. The cDNA was synthesized using a reverse primer (5′-ATT GAT GGT GCC TAC AG-3′) complementary to the adaptor sequence and MMLV reverse transcriptase (Invitrogen).

    Amplification:

    Article Title:
    Article Snippet: A 5′-adenylated and 3′-blocked oligodeoxyribonucleotide microRNA (miRNA) cloning adaptor (5′ rApp CTG TAG GCA CCA TCA AT–NH2 3′; S1315; NEB) was ligated to the 3′ OH of the noncoding RNA using T4 RNA ligase 2 (M0351; NEB) by following the manufacturer's instructions. .. Then the 5′ cDNA end was PCR amplified using forward primer SP4FW (5′-GTG AAG GGT GAC TGT AGT CCT GAG C-3′; nt 5227 to 5251) and the reverse primer.

    Agarose Gel Electrophoresis:

    Article Title:
    Article Snippet: A 5′-adenylated and 3′-blocked oligodeoxyribonucleotide microRNA (miRNA) cloning adaptor (5′ rApp CTG TAG GCA CCA TCA AT–NH2 3′; S1315; NEB) was ligated to the 3′ OH of the noncoding RNA using T4 RNA ligase 2 (M0351; NEB) by following the manufacturer's instructions. .. PCR fragments were electrophoresed on a 1.5% agarose gel.

    Synthesized:

    Article Title:
    Article Snippet: A 5′-adenylated and 3′-blocked oligodeoxyribonucleotide microRNA (miRNA) cloning adaptor (5′ rApp CTG TAG GCA CCA TCA AT–NH2 3′; S1315; NEB) was ligated to the 3′ OH of the noncoding RNA using T4 RNA ligase 2 (M0351; NEB) by following the manufacturer's instructions. .. The cDNA was synthesized using a reverse primer (5′-ATT GAT GGT GCC TAC AG-3′) complementary to the adaptor sequence and MMLV reverse transcriptase (Invitrogen).

    CTG Assay:

    Article Title:
    Article Snippet: .. A 5′-adenylated and 3′-blocked oligodeoxyribonucleotide microRNA (miRNA) cloning adaptor (5′ rApp CTG TAG GCA CCA TCA AT–NH2 3′; S1315; NEB) was ligated to the 3′ OH of the noncoding RNA using T4 RNA ligase 2 (M0351; NEB) by following the manufacturer's instructions. .. The cDNA was synthesized using a reverse primer (5′-ATT GAT GGT GCC TAC AG-3′) complementary to the adaptor sequence and MMLV reverse transcriptase (Invitrogen).

    Sequencing:

    Article Title:
    Article Snippet: A 5′-adenylated and 3′-blocked oligodeoxyribonucleotide microRNA (miRNA) cloning adaptor (5′ rApp CTG TAG GCA CCA TCA AT–NH2 3′; S1315; NEB) was ligated to the 3′ OH of the noncoding RNA using T4 RNA ligase 2 (M0351; NEB) by following the manufacturer's instructions. .. The cDNA was synthesized using a reverse primer (5′-ATT GAT GGT GCC TAC AG-3′) complementary to the adaptor sequence and MMLV reverse transcriptase (Invitrogen).

    Polymerase Chain Reaction:

    Article Title:
    Article Snippet: A 5′-adenylated and 3′-blocked oligodeoxyribonucleotide microRNA (miRNA) cloning adaptor (5′ rApp CTG TAG GCA CCA TCA AT–NH2 3′; S1315; NEB) was ligated to the 3′ OH of the noncoding RNA using T4 RNA ligase 2 (M0351; NEB) by following the manufacturer's instructions. .. Then the 5′ cDNA end was PCR amplified using forward primer SP4FW (5′-GTG AAG GGT GAC TGT AGT CCT GAG C-3′; nt 5227 to 5251) and the reverse primer.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    New England Biolabs universal mirna cloning linker
    Universal Mirna Cloning Linker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/universal mirna cloning linker/product/New England Biolabs
    Average 90 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    universal mirna cloning linker - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    Image Search Results