random primer 9  (New England Biolabs)


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  • 99
    Name:
    Random Primer 9
    Description:
    Random Primer 9 1 0 A260 units
    Catalog Number:
    s1254s
    Price:
    99
    Size:
    1 0 A260 units
    Category:
    Probes and Primers
    Buy from Supplier


    Structured Review

    New England Biolabs random primer 9
    Random Primer 9
    Random Primer 9 1 0 A260 units
    https://www.bioz.com/result/random primer 9/product/New England Biolabs
    Average 99 stars, based on 52 article reviews
    Price from $9.99 to $1999.99
    random primer 9 - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Generated:

    Article Title: Spatiotemporal regulation of the GPCR activity of BAI3 by C1qL4 and Stabilin-2 controls myoblast fusion
    Article Snippet: .. Total RNAs were treated with DNAse1 (Invitrogen) and cDNAs were generated using the M-MuLV Reverse Transcriptase and random primers (NEB), as recommended by the manufacturer. .. Specific knockdown of the genes of interest was confirmed by real-time Q-PCR in an Mx3005P (Stratagene) system using SYBR Green PCR Master Mix (Applied Biosystems).

    Synthesized:

    Article Title: RsmV, a Small Noncoding Regulatory RNA in Pseudomonas aeruginosa That Sequesters RsmA and RsmF from Target mRNAs
    Article Snippet: .. First-strand cDNA was synthesized using Superscript II (Thermo Fisher) according to the manufacturer's protocol with Random Primer 9 (NEB). .. The copy number of the indicated genes was determined by qPCR using SYBR green master mix (Bio-Rad).

    Incubation:

    Article Title: Structural divergence creates new functional features in alphavirus genomes
    Article Snippet: .. Total purified RNAs were then incubated with 500 ng Random Primer 9 (NEB) at 65°C for 5 min, cooled to 0°C, and mixed with 10 mM dNTPs, 0.1 M DTT, 500 mM Tris pH 8.0, 750 KCl and 500 mM MnCl2 . .. The mix was then incubated at 42°C for 2 min, followed by the addition of 200 units of SuperScript II (Thermo Fisher Scientific) and a further incubation at 42°C for 180 min, heat inactivated at 70°C for 15 min, and then purified using illustra MicroSpin G-50 columns.

    Spectrophotometry:

    Article Title: A Chemical Genetics Screen Reveals Influence of p38 Mitogen-Activated Protein Kinase and Autophagy on Phagosome Development and Intracellular Replication of Brucella neotomae in Macrophages
    Article Snippet: .. Equivalent amounts of cellular RNA were analyzed based on Nanodrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA) measurements. cDNA synthesis was performed using Moloney murine leukemia virus (M-MuLV) reverse transcriptase with a random primer mix (New England Biolabs, New Bedford, MA). .. The DyNAmo HS SYBR green qPCR kit (Thermo Fisher Scientific) was used for qPCR reactions.

    Purification:

    Article Title: Structural divergence creates new functional features in alphavirus genomes
    Article Snippet: .. Total purified RNAs were then incubated with 500 ng Random Primer 9 (NEB) at 65°C for 5 min, cooled to 0°C, and mixed with 10 mM dNTPs, 0.1 M DTT, 500 mM Tris pH 8.0, 750 KCl and 500 mM MnCl2 . .. The mix was then incubated at 42°C for 2 min, followed by the addition of 200 units of SuperScript II (Thermo Fisher Scientific) and a further incubation at 42°C for 180 min, heat inactivated at 70°C for 15 min, and then purified using illustra MicroSpin G-50 columns.

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  • 99
    New England Biolabs random primer 9
    Random Primer 9, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/random primer 9/product/New England Biolabs
    Average 99 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    random primer 9 - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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