random primer 9  (New England Biolabs)


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  • 99
    Name:
    Random Primer 9
    Description:
    Random Primer 9 1 0 A260 units
    Catalog Number:
    s1254s
    Price:
    99
    Size:
    1 0 A260 units
    Category:
    Probes and Primers
    Buy from Supplier


    Structured Review

    New England Biolabs random primer 9
    Random Primer 9
    Random Primer 9 1 0 A260 units
    https://www.bioz.com/result/random primer 9/product/New England Biolabs
    Average 99 stars, based on 56 article reviews
    Price from $9.99 to $1999.99
    random primer 9 - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Human Guanylate Binding Proteins Potentiate the Anti-Chlamydia Effects of Interferon-?
    Article Snippet: .. Real time PCR Expression of human Guanylate Binding Protein (GBP)-1, GBP2 and β-actin was assessed by isolating total RNA from 1×106 HeLa cells using the TRIZOL reagent per the manufacturer's instructions, followed by reverse transcription ( 500 ng total RNA, Superscript III Reverse Transcriptase system (Invitrogen cat #18080-044), nonamer Random Primer 9 (New England Biolabs cat #S1254S)) and quantitative real-time polymerase chain reaction PCR. .. The QuantiTect primers for the gene GBP-1 (NM_002053; cat #QT01669885 or #QT00011641) with a predicted amplicon length of 96 bp and custom designed primers for GBP2 (FWD 5′-GAC CAA ATG TTC CAG AGG AAA TTA GGG GC-3′ , REV 5′-AAT GTT CCC TGC TTG ACA TCT TCT TCT AAA GG-3′ ) were used.

    Amplification:

    Article Title: Sexual and asexual oogenesis require the expression of unique and shared sets of genes in the insect Acyrthosiphon pisum
    Article Snippet: .. First strand cDNAs were produced from 1 μg of total RNAs by using random primer 9 (New England BioLabs) and SuperScript® III Reverse Transcriptase (Invitrogen) following the supplier's instructions. cDNAs were used as a matrix for PCR amplification with specific primers (Additional file ). .. Amplified fragments were cloned into the StrataClone PCR Cloning Vector pSC-A-amp/kan (StrataClone) or pENTR Directional TOPO (Invitrogen) and sequenced (Genoscreen).

    Synthesized:

    Article Title: RsmV, a Small Noncoding Regulatory RNA in Pseudomonas aeruginosa That Sequesters RsmA and RsmF from Target mRNAs
    Article Snippet: .. First-strand cDNA was synthesized using Superscript II (Thermo Fisher) according to the manufacturer's protocol with Random Primer 9 (NEB). .. The copy number of the indicated genes was determined by qPCR using SYBR green master mix (Bio-Rad).

    Isolation:

    Article Title: Puumala and Tula Virus Differ in Replication Kinetics and Innate Immune Stimulation in Human Endothelial Cells and Macrophages
    Article Snippet: .. Isolated RNA from cultivated cells was converted to cDNA using SuperScript IV reverse transcriptase (Thermo Fisher Scientific) and random primer mix (New England Biolabs, Ipswich, MA, USA). .. Analysis of host cell gene expression was performed using commercially available TaqMan® Gene Expression Assays (Thermo Fisher Scientific; IFNB1 (Hs01077958_s1); IFNA1 (Hs04189288_g1); IFNAR1 (Hs01066116_m1); IFNAR2 (Hs01022059_m1); IFNE (Hs00703565_s1); IFNK (Hs00737883_m1); IFNW1 (Hs00958789_s1); IFNL1 (Hs00601677_g1); IFNL2 (Hs00820125_g1); IFNG (Hs00989291_m1).

    Generated:

    Article Title: Spatiotemporal regulation of the GPCR activity of BAI3 by C1qL4 and Stabilin-2 controls myoblast fusion
    Article Snippet: .. Total RNAs were treated with DNAse1 (Invitrogen) and cDNAs were generated using the M-MuLV Reverse Transcriptase and random primers (NEB), as recommended by the manufacturer. .. Specific knockdown of the genes of interest was confirmed by real-time Q-PCR in an Mx3005P (Stratagene) system using SYBR Green PCR Master Mix (Applied Biosystems).

    Purification:

    Article Title: Structural divergence creates new functional features in alphavirus genomes
    Article Snippet: .. Total purified RNAs were then incubated with 500 ng Random Primer 9 (NEB) at 65°C for 5 min, cooled to 0°C, and mixed with 10 mM dNTPs, 0.1 M DTT, 500 mM Tris pH 8.0, 750 KCl and 500 mM MnCl2 . .. The mix was then incubated at 42°C for 2 min, followed by the addition of 200 units of SuperScript II (Thermo Fisher Scientific) and a further incubation at 42°C for 180 min, heat inactivated at 70°C for 15 min, and then purified using illustra MicroSpin G-50 columns.

    Produced:

    Article Title: Sexual and asexual oogenesis require the expression of unique and shared sets of genes in the insect Acyrthosiphon pisum
    Article Snippet: .. First strand cDNAs were produced from 1 μg of total RNAs by using random primer 9 (New England BioLabs) and SuperScript® III Reverse Transcriptase (Invitrogen) following the supplier's instructions. cDNAs were used as a matrix for PCR amplification with specific primers (Additional file ). .. Amplified fragments were cloned into the StrataClone PCR Cloning Vector pSC-A-amp/kan (StrataClone) or pENTR Directional TOPO (Invitrogen) and sequenced (Genoscreen).

    Incubation:

    Article Title: Structural divergence creates new functional features in alphavirus genomes
    Article Snippet: .. Total purified RNAs were then incubated with 500 ng Random Primer 9 (NEB) at 65°C for 5 min, cooled to 0°C, and mixed with 10 mM dNTPs, 0.1 M DTT, 500 mM Tris pH 8.0, 750 KCl and 500 mM MnCl2 . .. The mix was then incubated at 42°C for 2 min, followed by the addition of 200 units of SuperScript II (Thermo Fisher Scientific) and a further incubation at 42°C for 180 min, heat inactivated at 70°C for 15 min, and then purified using illustra MicroSpin G-50 columns.

    Expressing:

    Article Title: Human Guanylate Binding Proteins Potentiate the Anti-Chlamydia Effects of Interferon-?
    Article Snippet: .. Real time PCR Expression of human Guanylate Binding Protein (GBP)-1, GBP2 and β-actin was assessed by isolating total RNA from 1×106 HeLa cells using the TRIZOL reagent per the manufacturer's instructions, followed by reverse transcription ( 500 ng total RNA, Superscript III Reverse Transcriptase system (Invitrogen cat #18080-044), nonamer Random Primer 9 (New England Biolabs cat #S1254S)) and quantitative real-time polymerase chain reaction PCR. .. The QuantiTect primers for the gene GBP-1 (NM_002053; cat #QT01669885 or #QT00011641) with a predicted amplicon length of 96 bp and custom designed primers for GBP2 (FWD 5′-GAC CAA ATG TTC CAG AGG AAA TTA GGG GC-3′ , REV 5′-AAT GTT CCC TGC TTG ACA TCT TCT TCT AAA GG-3′ ) were used.

    Polymerase Chain Reaction:

    Article Title: Human Guanylate Binding Proteins Potentiate the Anti-Chlamydia Effects of Interferon-?
    Article Snippet: .. Real time PCR Expression of human Guanylate Binding Protein (GBP)-1, GBP2 and β-actin was assessed by isolating total RNA from 1×106 HeLa cells using the TRIZOL reagent per the manufacturer's instructions, followed by reverse transcription ( 500 ng total RNA, Superscript III Reverse Transcriptase system (Invitrogen cat #18080-044), nonamer Random Primer 9 (New England Biolabs cat #S1254S)) and quantitative real-time polymerase chain reaction PCR. .. The QuantiTect primers for the gene GBP-1 (NM_002053; cat #QT01669885 or #QT00011641) with a predicted amplicon length of 96 bp and custom designed primers for GBP2 (FWD 5′-GAC CAA ATG TTC CAG AGG AAA TTA GGG GC-3′ , REV 5′-AAT GTT CCC TGC TTG ACA TCT TCT TCT AAA GG-3′ ) were used.

    Article Title: Sexual and asexual oogenesis require the expression of unique and shared sets of genes in the insect Acyrthosiphon pisum
    Article Snippet: .. First strand cDNAs were produced from 1 μg of total RNAs by using random primer 9 (New England BioLabs) and SuperScript® III Reverse Transcriptase (Invitrogen) following the supplier's instructions. cDNAs were used as a matrix for PCR amplification with specific primers (Additional file ). .. Amplified fragments were cloned into the StrataClone PCR Cloning Vector pSC-A-amp/kan (StrataClone) or pENTR Directional TOPO (Invitrogen) and sequenced (Genoscreen).

    Binding Assay:

    Article Title: Human Guanylate Binding Proteins Potentiate the Anti-Chlamydia Effects of Interferon-?
    Article Snippet: .. Real time PCR Expression of human Guanylate Binding Protein (GBP)-1, GBP2 and β-actin was assessed by isolating total RNA from 1×106 HeLa cells using the TRIZOL reagent per the manufacturer's instructions, followed by reverse transcription ( 500 ng total RNA, Superscript III Reverse Transcriptase system (Invitrogen cat #18080-044), nonamer Random Primer 9 (New England Biolabs cat #S1254S)) and quantitative real-time polymerase chain reaction PCR. .. The QuantiTect primers for the gene GBP-1 (NM_002053; cat #QT01669885 or #QT00011641) with a predicted amplicon length of 96 bp and custom designed primers for GBP2 (FWD 5′-GAC CAA ATG TTC CAG AGG AAA TTA GGG GC-3′ , REV 5′-AAT GTT CCC TGC TTG ACA TCT TCT TCT AAA GG-3′ ) were used.

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  • About
  • News
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  • Team
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  • Bioz Stars
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  • 99
    New England Biolabs random primer 9
    Random Primer 9, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/random primer 9/product/New England Biolabs
    Average 99 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    random primer 9 - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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