primer 6  (New England Biolabs)


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  • 97
    Name:
    Random Primer 6
    Description:
    Random Primer 6 1 0 A260 units
    Catalog Number:
    s1230s
    Price:
    99
    Size:
    1 0 A260 units
    Category:
    Probes and Primers
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    Structured Review

    New England Biolabs primer 6
    Random Primer 6
    Random Primer 6 1 0 A260 units
    https://www.bioz.com/result/primer 6/product/New England Biolabs
    Average 97 stars, based on 139 article reviews
    Price from $9.99 to $1999.99
    primer 6 - by Bioz Stars, 2020-09
    97/100 stars

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    Related Articles

    Amplification:

    Article Title: Construction and application of a novel hepatocyte-directed vector to simultaneous knockdown and overexpression of multiple genes.
    Article Snippet: .. Liver disease, such as malignancy and hepatitis, often correlates with several genetic disorders. ..

    RNA Sequencing Assay:

    Article Title: Genomics and transcriptomics of Xanthomonas campestris species challenge the concept of core type III effectome
    Article Snippet: .. Quantitative RT-PCR analyses Two micrograms of total RNA used for the RNA sequencing were subjected to reverse transcription using Transcriptor reverse trancriptase (RT, Roche Applied Science) and 1 μM Random Primer 6 (New England BioLabs). .. Quantitative PCR was performed using diluted cDNA with 1 μM gene-specific oligonucleotides (Additional file ) and LightCycler 480 SYBR Green I Master kit on a LightCycler 480 (Roche Applied Science) machine with the following cycling parameters: 9 min 95 °C; 50 cycles of 15 sec 95 °C, 10 sec 60 °C and 10 sec 72 °C.

    Synthesized:

    Article Title: Detection of the Emerging Picornavirus Senecavirus A in Pigs, Mice, and Houseflies
    Article Snippet: .. Viral cDNA was synthesized using ProtoScript II reverse transcriptase (New England BioLabs, Ipswich, MA) and a mixture of oligo d(T)18 and random primer 6 (NEB, Ipswich, MA). .. Two microliters of cDNA was used in PCR amplification reactions with the Q5 hot start high-fidelity 2× master mix (NEB, Ipswich, MA) by following the manufacturer's protocols.

    Quantitative RT-PCR:

    Article Title: Genomics and transcriptomics of Xanthomonas campestris species challenge the concept of core type III effectome
    Article Snippet: .. Quantitative RT-PCR analyses Two micrograms of total RNA used for the RNA sequencing were subjected to reverse transcription using Transcriptor reverse trancriptase (RT, Roche Applied Science) and 1 μM Random Primer 6 (New England BioLabs). .. Quantitative PCR was performed using diluted cDNA with 1 μM gene-specific oligonucleotides (Additional file ) and LightCycler 480 SYBR Green I Master kit on a LightCycler 480 (Roche Applied Science) machine with the following cycling parameters: 9 min 95 °C; 50 cycles of 15 sec 95 °C, 10 sec 60 °C and 10 sec 72 °C.

    Polymerase Chain Reaction:

    Article Title: Construction and application of a novel hepatocyte-directed vector to simultaneous knockdown and overexpression of multiple genes.
    Article Snippet: .. Liver disease, such as malignancy and hepatitis, often correlates with several genetic disorders. ..

    Article Title: Moraxella catarrhalis phase-variable loci show differences in expression during conditions relevant to disease
    Article Snippet: .. RNA was subsequently treated with DNaseI (NEB) and the absence of contaminating DNA was confirmed by PCR using GoTaq polymerase (Promega) and copB primers ( ). cDNA was prepared using ProtoScript II Reverse Transcriptase (NEB), with random Primer 6 (NEB) and 1 μg RNA. .. 2.5 ng of cDNA was used as the template in 20 μl qRT-PCR reactions with SsoAdvanced Universal SYBR Green Supermix (Biorad). qRT-PCR reactions were performed in triplicate using a CFX96 Real-Time PCR Detection System (Biorad), and primers for these reactions are listed in .

    Random Hexamer Labeling:

    Article Title: Nanopore sequencing as a revolutionary diagnostic tool for porcine viral enteric disease complexes identifies porcine kobuvirus as an important enteric virus
    Article Snippet: .. Ten microliters of template nucleic acids were mixed with 0.5 µl random hexamer primers (Random Primer 6, New England Biolabs), 1 µl dNTP mix (NEB) and 2.5 µl nuclease-free water. .. Primer annealing was conducted at 65 °C for 5 min, after which 4 µl Superscript IV Reaction Buffer (ThermoScientific), 1 µl dithiothreitol (ThermoScientific) and 1 µl SuperScript IV Reverse Transcriptase (ThermoScientific) were added in a total reaction volume of 20 µl.

    shRNA:

    Article Title: Construction and application of a novel hepatocyte-directed vector to simultaneous knockdown and overexpression of multiple genes.
    Article Snippet: .. Liver disease, such as malignancy and hepatitis, often correlates with several genetic disorders. ..

    Expressing:

    Article Title: Construction and application of a novel hepatocyte-directed vector to simultaneous knockdown and overexpression of multiple genes.
    Article Snippet: .. Liver disease, such as malignancy and hepatitis, often correlates with several genetic disorders. ..

    Modification:

    Article Title: Construction and application of a novel hepatocyte-directed vector to simultaneous knockdown and overexpression of multiple genes.
    Article Snippet: .. Liver disease, such as malignancy and hepatitis, often correlates with several genetic disorders. ..

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  • 97
    New England Biolabs primer 6
    Primer 6, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primer 6/product/New England Biolabs
    Average 97 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    primer 6 - by Bioz Stars, 2020-09
    97/100 stars
      Buy from Supplier

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