rtu kit (Vector Laboratories)

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Vector Laboratories rtu kit
Rtu Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rtu kit/product/Vector Laboratories
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99

rtu kit - by Bioz Stars, 2021-03

93/100 stars

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Activity Assay:

Article Title: Hepcidin as a Major Component of Renal Antibacterial Defenses against Uropathogenic Escherichia coli
Article Snippet: Antigen retrieval from kidney sections was performed in citrate buffer at pH 6 (Vector Laboratories, Burlingame, CA) in a steamer. .. Endogenous peroxidase activity was blocked with 3% H2 O2 , and nonspecific protein binding was blocked with serumfree protein blocking solution (RTU Kit; Vector Laboratories). .. Kidney sections were first incubated overnight at 4°C with primary anti-F4/80 monoclonal antibody (AbD Serotec, a Bio-Rad company, Marnes-la-Coquette, France) applied at 1:50 dilution and incubated.

Protein Binding:

Blocking Assay:

Incubation:

Article Title: Nordihydroguaiaretic acid (NDGA) inhibits ritonavir-induced endothelial dysfunction in porcine pulmonary arteries
Article Snippet: Samples were then sectioned at a 5-μm thickness and slides were incubated with monoclonal antibody against human eNOS (1:500) for 30 min at room temperature. .. After being washed with PBS, the slides were incubated with biotinylated secondary antibody for 30 min. Then, an avidin-biotin reaction using peroxidase enzyme was used for protein detection (ABC kit; Vector Laboratories, Burlingham, CA). .. ROS measurement Levels of O2 − produced by the endothelial cells of porcine arteries were detected using the lucigenin-enhanced chemiluminescence method previously described in our studies [ , ].

Article Title: Transplantation of Human Neural Stem Cells in a Parkinsonian Model Exerts Neuroprotection via Regulation of the Host Microenvironment
Article Snippet: Brain slices were processed free floating, pretreated for peroxidase activity using 3% hydrogen peroxide (H2 O2 ) in 0.1 M PBS containing 5% Triton X-100 for 20 min, and blocked with 5% normal horse serum (NHS) (Vector laboratories, Burlingame, CA, USA) for 1 h. Thereafter, selected tissue slices were incubated with primary antibodies of TH (1:800, Millipore, Billerica, MA, USA), GFAP (1:600, Millipore, Billerica, MA, USA), and IBA-1 (1:400, Wako, Chūō-ku, Osaka, Japan) at 4 °C overnight. .. Slices were again washed 3 times with 0.1 M PBS, incubated with biotinylated horse anti-rabbit IgG (Vector laboratories, Burlingame, CA, USA) or goat anti-chicken IgG (Vector laboratories, Burlingame, CA, USA) secondary antibody for 1 h, and then treated with biotinylated protein A and avidinbiotinylated horseradish peroxidase complexes (ABC kit, Vector laboratories, Burlingame, CA, USA) for another 1 h at room temperature. .. Immunoreactive cells were visualized with 3,3′-diaminobenzidine (DAB) and urea H2 O2 tablets (Sigma-Aldrich, St. Louis, MO, USA) dissolved in double distilled water.

Article Title: Gene Profile Identifies Zinc Transporters Differentially Expressed in Normal Human Organs and Human Pancreatic Cancer
Article Snippet: .. After washing with PBS, the section was incubated with biotinylated secondary antibody for 30 min. An avidin-biotin reaction using peroxidase enzyme was used for protein detection (ABC kit; Vector Laboratories, Burlingham, CA). .. Immune complexes were detected with diaminobenzidine (DAB) under a phase contrast microscope.

Avidin-Biotin Assay:

Immunohistochemistry:

Article Title: Intermittent PTH administration improves alveolar bone formation in type 1 diabetic rats with periodontitis
Article Snippet: To inhibit endogenous peroxidase activity, sections were quenched in 3% H2 O2 for 20 min and then treated with trypsin (Thermo Fisher Scientific, Waltham, MA, USA) for antigen retrieval. .. Immunohistochemistry was carried out using universal kits (Vector Laboratories, Burlingame, CA, USA) according to the manufacturer’s instructions. .. Sections were preincubated with normal horse blocking solution for 20 min and then incubated overnight at 4 °C with goat anti-sclerostin antibody (1:300 dilution, R & D Systems, Minneapolis, MN, USA).