collagen iii  (Boster Bio)


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    Structured Review

    Boster Bio collagen iii
    NaHS blocked <t>collagen</t> synthesis in keloid fibroblasts. Skin fibroblasts were extracted from skin tissues of normal controls (normal skin) and scar tissue from patients with keloid (keloid), respectively. After pretreatment with NaHS (50 μ M) or the same amount of culture medium (control) for 4 h, the fresh culture medium was replaced. (a) After 12 h, collagen I mRNA expression was detected with real-time PCR. (b) Collagen I was immunofluorescence stained with Cy3- (red) conjugated IgG. The nuclei were stained with DAPI (blue). Bar = 75 μ m. (c) <t>Collagen</t> <t>III</t> mRNA expression was detected with real-time PCR. (d) Collagen III was immunofluorescence stained with Cy3- (red) conjugated IgG. The nuclei were stained with DAPI (blue). Bar = 75 μ m. ∗∗ P
    Collagen Iii, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagen iii/product/Boster Bio
    Average 94 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    collagen iii - by Bioz Stars, 2022-12
    94/100 stars

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    1) Product Images from "Hydrogen Sulfide Suppresses Skin Fibroblast Proliferation via Oxidative Stress Alleviation and Necroptosis Inhibition"

    Article Title: Hydrogen Sulfide Suppresses Skin Fibroblast Proliferation via Oxidative Stress Alleviation and Necroptosis Inhibition

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2022/7434733

    NaHS blocked collagen synthesis in keloid fibroblasts. Skin fibroblasts were extracted from skin tissues of normal controls (normal skin) and scar tissue from patients with keloid (keloid), respectively. After pretreatment with NaHS (50 μ M) or the same amount of culture medium (control) for 4 h, the fresh culture medium was replaced. (a) After 12 h, collagen I mRNA expression was detected with real-time PCR. (b) Collagen I was immunofluorescence stained with Cy3- (red) conjugated IgG. The nuclei were stained with DAPI (blue). Bar = 75 μ m. (c) Collagen III mRNA expression was detected with real-time PCR. (d) Collagen III was immunofluorescence stained with Cy3- (red) conjugated IgG. The nuclei were stained with DAPI (blue). Bar = 75 μ m. ∗∗ P
    Figure Legend Snippet: NaHS blocked collagen synthesis in keloid fibroblasts. Skin fibroblasts were extracted from skin tissues of normal controls (normal skin) and scar tissue from patients with keloid (keloid), respectively. After pretreatment with NaHS (50 μ M) or the same amount of culture medium (control) for 4 h, the fresh culture medium was replaced. (a) After 12 h, collagen I mRNA expression was detected with real-time PCR. (b) Collagen I was immunofluorescence stained with Cy3- (red) conjugated IgG. The nuclei were stained with DAPI (blue). Bar = 75 μ m. (c) Collagen III mRNA expression was detected with real-time PCR. (d) Collagen III was immunofluorescence stained with Cy3- (red) conjugated IgG. The nuclei were stained with DAPI (blue). Bar = 75 μ m. ∗∗ P

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining

    NaHS prevented collagen synthesis in TGF- β 1 -stimulated fibroblasts. Skin fibroblasts were extracted from skin tissues of normal controls (normal skin). After pretreatment with NaHS (50 μ M) for 4 h, skin fibroblasts were stimulated with TGF- β 1 (10 ng/mL) for 12 h. (a) Collagen I mRNA expression was detected with real-time PCR. (b) Collagen I was immunofluorescence stained with Alexa Fluor 488- (green) conjugated IgG. The nuclei were stained with DAPI (blue). Bar = 25 μ m. (c) Collagen III mRNA expression was detected with real-time PCR. (d) Collagen III was immunofluorescence stained with Alexa Fluor 488- (green) conjugated IgG. The nuclei were stained with DAPI (blue). Bar = 25 μ m. ∗∗ P
    Figure Legend Snippet: NaHS prevented collagen synthesis in TGF- β 1 -stimulated fibroblasts. Skin fibroblasts were extracted from skin tissues of normal controls (normal skin). After pretreatment with NaHS (50 μ M) for 4 h, skin fibroblasts were stimulated with TGF- β 1 (10 ng/mL) for 12 h. (a) Collagen I mRNA expression was detected with real-time PCR. (b) Collagen I was immunofluorescence stained with Alexa Fluor 488- (green) conjugated IgG. The nuclei were stained with DAPI (blue). Bar = 25 μ m. (c) Collagen III mRNA expression was detected with real-time PCR. (d) Collagen III was immunofluorescence stained with Alexa Fluor 488- (green) conjugated IgG. The nuclei were stained with DAPI (blue). Bar = 25 μ m. ∗∗ P

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining

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  • 94
    Boster Bio collagen iii
    NaHS blocked <t>collagen</t> synthesis in keloid fibroblasts. Skin fibroblasts were extracted from skin tissues of normal controls (normal skin) and scar tissue from patients with keloid (keloid), respectively. After pretreatment with NaHS (50 μ M) or the same amount of culture medium (control) for 4 h, the fresh culture medium was replaced. (a) After 12 h, collagen I mRNA expression was detected with real-time PCR. (b) Collagen I was immunofluorescence stained with Cy3- (red) conjugated IgG. The nuclei were stained with DAPI (blue). Bar = 75 μ m. (c) <t>Collagen</t> <t>III</t> mRNA expression was detected with real-time PCR. (d) Collagen III was immunofluorescence stained with Cy3- (red) conjugated IgG. The nuclei were stained with DAPI (blue). Bar = 75 μ m. ∗∗ P
    Collagen Iii, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagen iii/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagen iii - by Bioz Stars, 2022-12
    94/100 stars
      Buy from Supplier

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    NaHS blocked collagen synthesis in keloid fibroblasts. Skin fibroblasts were extracted from skin tissues of normal controls (normal skin) and scar tissue from patients with keloid (keloid), respectively. After pretreatment with NaHS (50 μ M) or the same amount of culture medium (control) for 4 h, the fresh culture medium was replaced. (a) After 12 h, collagen I mRNA expression was detected with real-time PCR. (b) Collagen I was immunofluorescence stained with Cy3- (red) conjugated IgG. The nuclei were stained with DAPI (blue). Bar = 75 μ m. (c) Collagen III mRNA expression was detected with real-time PCR. (d) Collagen III was immunofluorescence stained with Cy3- (red) conjugated IgG. The nuclei were stained with DAPI (blue). Bar = 75 μ m. ∗∗ P

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Hydrogen Sulfide Suppresses Skin Fibroblast Proliferation via Oxidative Stress Alleviation and Necroptosis Inhibition

    doi: 10.1155/2022/7434733

    Figure Lengend Snippet: NaHS blocked collagen synthesis in keloid fibroblasts. Skin fibroblasts were extracted from skin tissues of normal controls (normal skin) and scar tissue from patients with keloid (keloid), respectively. After pretreatment with NaHS (50 μ M) or the same amount of culture medium (control) for 4 h, the fresh culture medium was replaced. (a) After 12 h, collagen I mRNA expression was detected with real-time PCR. (b) Collagen I was immunofluorescence stained with Cy3- (red) conjugated IgG. The nuclei were stained with DAPI (blue). Bar = 75 μ m. (c) Collagen III mRNA expression was detected with real-time PCR. (d) Collagen III was immunofluorescence stained with Cy3- (red) conjugated IgG. The nuclei were stained with DAPI (blue). Bar = 75 μ m. ∗∗ P

    Article Snippet: After incubation with α -smooth muscle actin (α-SMA, 1 : 100; Bosterbio, Wuhan, China), proliferating cell nuclear antigen (PCNA, 1 : 100; Abclonal, Wuhan, China), and collagen I and collagen III (1 : 100; Bosterbio, Wuhan, China) antibodies overnight at 4°C, skin fibroblasts were incubated with Cy3- or Alexa Fluor 488-conjugated IgG (1 : 500; Beyotime, Shanghai, China) for 2 h in the dark.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining

    NaHS prevented collagen synthesis in TGF- β 1 -stimulated fibroblasts. Skin fibroblasts were extracted from skin tissues of normal controls (normal skin). After pretreatment with NaHS (50 μ M) for 4 h, skin fibroblasts were stimulated with TGF- β 1 (10 ng/mL) for 12 h. (a) Collagen I mRNA expression was detected with real-time PCR. (b) Collagen I was immunofluorescence stained with Alexa Fluor 488- (green) conjugated IgG. The nuclei were stained with DAPI (blue). Bar = 25 μ m. (c) Collagen III mRNA expression was detected with real-time PCR. (d) Collagen III was immunofluorescence stained with Alexa Fluor 488- (green) conjugated IgG. The nuclei were stained with DAPI (blue). Bar = 25 μ m. ∗∗ P

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Hydrogen Sulfide Suppresses Skin Fibroblast Proliferation via Oxidative Stress Alleviation and Necroptosis Inhibition

    doi: 10.1155/2022/7434733

    Figure Lengend Snippet: NaHS prevented collagen synthesis in TGF- β 1 -stimulated fibroblasts. Skin fibroblasts were extracted from skin tissues of normal controls (normal skin). After pretreatment with NaHS (50 μ M) for 4 h, skin fibroblasts were stimulated with TGF- β 1 (10 ng/mL) for 12 h. (a) Collagen I mRNA expression was detected with real-time PCR. (b) Collagen I was immunofluorescence stained with Alexa Fluor 488- (green) conjugated IgG. The nuclei were stained with DAPI (blue). Bar = 25 μ m. (c) Collagen III mRNA expression was detected with real-time PCR. (d) Collagen III was immunofluorescence stained with Alexa Fluor 488- (green) conjugated IgG. The nuclei were stained with DAPI (blue). Bar = 25 μ m. ∗∗ P

    Article Snippet: After incubation with α -smooth muscle actin (α-SMA, 1 : 100; Bosterbio, Wuhan, China), proliferating cell nuclear antigen (PCNA, 1 : 100; Abclonal, Wuhan, China), and collagen I and collagen III (1 : 100; Bosterbio, Wuhan, China) antibodies overnight at 4°C, skin fibroblasts were incubated with Cy3- or Alexa Fluor 488-conjugated IgG (1 : 500; Beyotime, Shanghai, China) for 2 h in the dark.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining