ifnγ  (Kingfisher Biotech)


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    Structured Review

    Kingfisher Biotech ifnγ
    Direct stimulation of canine ASCs and PMSCs leads to production of IDO and PGE 2 . Canine adipose-derived MSCs ( ASCs ) and placenta-derived MSCs ( PMSCs ) secrete increased levels of indoleamine 2,3 dioxygenase (IDO) activity and prostaglandin E 2 (PGE 2 ) in response to direct stimulation using recombinant interferon gamma <t>(IFNγ)</t> and tumor necrosis factor alpha (TNFα ) . a IDO activity is directly proportional to the conversion of tryptophan to N -formyl kynurenine. ASCs and PMSCs increase IDO activity in response to dual stimulation with IFNγ and TNFα. IFNγ is the main contributor to the production of IDO. IFNγ- and TNFα-stimulated ASCs promote significantly higher levels of IDO activity as compared to PMSCs. b Canine ASCs and PMSCs produce comparable levels of prostaglandin E 2 (PGE 2 ) in response to dual stimulation with IFNγ and TNFα. TNFα is the major contributor to MSC-mediated PGE 2 production. Data presented as mean and standard error. * p
    Ifnγ, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ifnγ/product/Kingfisher Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ifnγ - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Placenta-derived multipotent mesenchymal stromal cells: a promising potential cell-based therapy for canine inflammatory brain disease"

    Article Title: Placenta-derived multipotent mesenchymal stromal cells: a promising potential cell-based therapy for canine inflammatory brain disease

    Journal: Stem Cell Research & Therapy

    doi: 10.1186/s13287-020-01799-0

    Direct stimulation of canine ASCs and PMSCs leads to production of IDO and PGE 2 . Canine adipose-derived MSCs ( ASCs ) and placenta-derived MSCs ( PMSCs ) secrete increased levels of indoleamine 2,3 dioxygenase (IDO) activity and prostaglandin E 2 (PGE 2 ) in response to direct stimulation using recombinant interferon gamma (IFNγ) and tumor necrosis factor alpha (TNFα ) . a IDO activity is directly proportional to the conversion of tryptophan to N -formyl kynurenine. ASCs and PMSCs increase IDO activity in response to dual stimulation with IFNγ and TNFα. IFNγ is the main contributor to the production of IDO. IFNγ- and TNFα-stimulated ASCs promote significantly higher levels of IDO activity as compared to PMSCs. b Canine ASCs and PMSCs produce comparable levels of prostaglandin E 2 (PGE 2 ) in response to dual stimulation with IFNγ and TNFα. TNFα is the major contributor to MSC-mediated PGE 2 production. Data presented as mean and standard error. * p
    Figure Legend Snippet: Direct stimulation of canine ASCs and PMSCs leads to production of IDO and PGE 2 . Canine adipose-derived MSCs ( ASCs ) and placenta-derived MSCs ( PMSCs ) secrete increased levels of indoleamine 2,3 dioxygenase (IDO) activity and prostaglandin E 2 (PGE 2 ) in response to direct stimulation using recombinant interferon gamma (IFNγ) and tumor necrosis factor alpha (TNFα ) . a IDO activity is directly proportional to the conversion of tryptophan to N -formyl kynurenine. ASCs and PMSCs increase IDO activity in response to dual stimulation with IFNγ and TNFα. IFNγ is the main contributor to the production of IDO. IFNγ- and TNFα-stimulated ASCs promote significantly higher levels of IDO activity as compared to PMSCs. b Canine ASCs and PMSCs produce comparable levels of prostaglandin E 2 (PGE 2 ) in response to dual stimulation with IFNγ and TNFα. TNFα is the major contributor to MSC-mediated PGE 2 production. Data presented as mean and standard error. * p

    Techniques Used: Derivative Assay, Activity Assay, Recombinant

    Related Articles

    Recombinant:

    Article Title: Characterization and Immunomodulatory Effects of Canine Adipose Tissue- and Bone Marrow-Derived Mesenchymal Stromal Cells
    Article Snippet: .. On day 5, 4 treatments of each MSC were designated: 1) 200 ng/mL recombinant canine interferon-gamma (IFN-γ, Kingfisher Biotech, Saint Paul, Minnesota, Cat# RP0271D-025) added, 2) 50 ng/mL recombinant canine tumour necrosis factor-alpha (TNF-α, R & D Systems, Minneapolis, Minnesota, Cat#1507-CT-025) added, 3) both IFN-γ and TNF-α added, and 4) neither IFN-γ nor TNF-α added. ..

    Article Title: Expression of PD-L1 on Canine Tumor Cells and Enhancement of IFN-γ Production from Tumor-Infiltrating Cells by PD-L1 Blockade
    Article Snippet: .. To stimulate the cells, the canine tumor cell lines were treated with 100 ng/mL canine recombinant IFN-γ (Kingfisher Biotech, St. Paul, MN, USA) and cultured for 24 h. Canine peripheral blood mononuclear cells (PBMCs) were purified from heparinized blood samples by density gradient centrifugation on Percoll (GE Healthcare UK, Buckinghamshire, UK) and cultured in RPMI 1640 medium supplemented with 10% FCS, 2 mM L-glutamine, 200 µg/mL streptomycin, and 200 U/mL penicillin at 37°C and 5% CO2 . ..

    Article Title: Placenta-derived multipotent mesenchymal stromal cells: a promising potential cell-based therapy for canine inflammatory brain disease
    Article Snippet: .. At plating, MSC was stimulated with IFNγ (50 ng/mL; canine recombinant IFNγ, Kingfisher, St. Paul, MN) or TNFα (50 ng/mL; canine recombinant TNFα, Kingfisher) or a dual stimulation with both IFNγ (50 ng/mL, Kingfisher) and TNFα (50 ng/mL, Kingfisher). ..

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Characterization and Immunomodulatory Effects of Canine Adipose Tissue- and Bone Marrow-Derived Mesenchymal Stromal Cells
    Article Snippet: .. On day 5, 4 treatments of each MSC were designated: 1) 200 ng/mL recombinant canine interferon-gamma (IFN-γ, Kingfisher Biotech, Saint Paul, Minnesota, Cat# RP0271D-025) added, 2) 50 ng/mL recombinant canine tumour necrosis factor-alpha (TNF-α, R & D Systems, Minneapolis, Minnesota, Cat#1507-CT-025) added, 3) both IFN-γ and TNF-α added, and 4) neither IFN-γ nor TNF-α added. ..

    Cell Culture:

    Article Title: Expression of PD-L1 on Canine Tumor Cells and Enhancement of IFN-γ Production from Tumor-Infiltrating Cells by PD-L1 Blockade
    Article Snippet: .. To stimulate the cells, the canine tumor cell lines were treated with 100 ng/mL canine recombinant IFN-γ (Kingfisher Biotech, St. Paul, MN, USA) and cultured for 24 h. Canine peripheral blood mononuclear cells (PBMCs) were purified from heparinized blood samples by density gradient centrifugation on Percoll (GE Healthcare UK, Buckinghamshire, UK) and cultured in RPMI 1640 medium supplemented with 10% FCS, 2 mM L-glutamine, 200 µg/mL streptomycin, and 200 U/mL penicillin at 37°C and 5% CO2 . ..

    Purification:

    Article Title: Expression of PD-L1 on Canine Tumor Cells and Enhancement of IFN-γ Production from Tumor-Infiltrating Cells by PD-L1 Blockade
    Article Snippet: .. To stimulate the cells, the canine tumor cell lines were treated with 100 ng/mL canine recombinant IFN-γ (Kingfisher Biotech, St. Paul, MN, USA) and cultured for 24 h. Canine peripheral blood mononuclear cells (PBMCs) were purified from heparinized blood samples by density gradient centrifugation on Percoll (GE Healthcare UK, Buckinghamshire, UK) and cultured in RPMI 1640 medium supplemented with 10% FCS, 2 mM L-glutamine, 200 µg/mL streptomycin, and 200 U/mL penicillin at 37°C and 5% CO2 . ..

    Gradient Centrifugation:

    Article Title: Expression of PD-L1 on Canine Tumor Cells and Enhancement of IFN-γ Production from Tumor-Infiltrating Cells by PD-L1 Blockade
    Article Snippet: .. To stimulate the cells, the canine tumor cell lines were treated with 100 ng/mL canine recombinant IFN-γ (Kingfisher Biotech, St. Paul, MN, USA) and cultured for 24 h. Canine peripheral blood mononuclear cells (PBMCs) were purified from heparinized blood samples by density gradient centrifugation on Percoll (GE Healthcare UK, Buckinghamshire, UK) and cultured in RPMI 1640 medium supplemented with 10% FCS, 2 mM L-glutamine, 200 µg/mL streptomycin, and 200 U/mL penicillin at 37°C and 5% CO2 . ..

    RNA Extraction:

    Article Title: 3D-printed nerve guidance conduits multi-functionalized with canine multipotent mesenchymal stromal cells promote neuroregeneration after sciatic nerve injury in rats
    Article Snippet: .. Subsequently, cells were stimulated with 0.75 mL basal medium containing IFN-γ (50 ng/mL, IFN- γ canine recombinant; Kingfisher Biotech, Saint Paul, USA) for 96 h. At this point, the cells were collected using TRIzol reagent (Invitrogen, São Paulo, Brazil) and stored at − 80 °C for RNA extraction and analysis of gene expression. ..

    Expressing:

    Article Title: 3D-printed nerve guidance conduits multi-functionalized with canine multipotent mesenchymal stromal cells promote neuroregeneration after sciatic nerve injury in rats
    Article Snippet: .. Subsequently, cells were stimulated with 0.75 mL basal medium containing IFN-γ (50 ng/mL, IFN- γ canine recombinant; Kingfisher Biotech, Saint Paul, USA) for 96 h. At this point, the cells were collected using TRIzol reagent (Invitrogen, São Paulo, Brazil) and stored at − 80 °C for RNA extraction and analysis of gene expression. ..

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  • News
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  • 94
    Kingfisher Biotech ifnγ
    Direct stimulation of canine ASCs and PMSCs leads to production of IDO and PGE 2 . Canine adipose-derived MSCs ( ASCs ) and placenta-derived MSCs ( PMSCs ) secrete increased levels of indoleamine 2,3 dioxygenase (IDO) activity and prostaglandin E 2 (PGE 2 ) in response to direct stimulation using recombinant interferon gamma <t>(IFNγ)</t> and tumor necrosis factor alpha (TNFα ) . a IDO activity is directly proportional to the conversion of tryptophan to N -formyl kynurenine. ASCs and PMSCs increase IDO activity in response to dual stimulation with IFNγ and TNFα. IFNγ is the main contributor to the production of IDO. IFNγ- and TNFα-stimulated ASCs promote significantly higher levels of IDO activity as compared to PMSCs. b Canine ASCs and PMSCs produce comparable levels of prostaglandin E 2 (PGE 2 ) in response to dual stimulation with IFNγ and TNFα. TNFα is the major contributor to MSC-mediated PGE 2 production. Data presented as mean and standard error. * p
    Ifnγ, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ifnγ/product/Kingfisher Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ifnγ - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Direct stimulation of canine ASCs and PMSCs leads to production of IDO and PGE 2 . Canine adipose-derived MSCs ( ASCs ) and placenta-derived MSCs ( PMSCs ) secrete increased levels of indoleamine 2,3 dioxygenase (IDO) activity and prostaglandin E 2 (PGE 2 ) in response to direct stimulation using recombinant interferon gamma (IFNγ) and tumor necrosis factor alpha (TNFα ) . a IDO activity is directly proportional to the conversion of tryptophan to N -formyl kynurenine. ASCs and PMSCs increase IDO activity in response to dual stimulation with IFNγ and TNFα. IFNγ is the main contributor to the production of IDO. IFNγ- and TNFα-stimulated ASCs promote significantly higher levels of IDO activity as compared to PMSCs. b Canine ASCs and PMSCs produce comparable levels of prostaglandin E 2 (PGE 2 ) in response to dual stimulation with IFNγ and TNFα. TNFα is the major contributor to MSC-mediated PGE 2 production. Data presented as mean and standard error. * p

    Journal: Stem Cell Research & Therapy

    Article Title: Placenta-derived multipotent mesenchymal stromal cells: a promising potential cell-based therapy for canine inflammatory brain disease

    doi: 10.1186/s13287-020-01799-0

    Figure Lengend Snippet: Direct stimulation of canine ASCs and PMSCs leads to production of IDO and PGE 2 . Canine adipose-derived MSCs ( ASCs ) and placenta-derived MSCs ( PMSCs ) secrete increased levels of indoleamine 2,3 dioxygenase (IDO) activity and prostaglandin E 2 (PGE 2 ) in response to direct stimulation using recombinant interferon gamma (IFNγ) and tumor necrosis factor alpha (TNFα ) . a IDO activity is directly proportional to the conversion of tryptophan to N -formyl kynurenine. ASCs and PMSCs increase IDO activity in response to dual stimulation with IFNγ and TNFα. IFNγ is the main contributor to the production of IDO. IFNγ- and TNFα-stimulated ASCs promote significantly higher levels of IDO activity as compared to PMSCs. b Canine ASCs and PMSCs produce comparable levels of prostaglandin E 2 (PGE 2 ) in response to dual stimulation with IFNγ and TNFα. TNFα is the major contributor to MSC-mediated PGE 2 production. Data presented as mean and standard error. * p

    Article Snippet: At plating, MSC was stimulated with IFNγ (50 ng/mL; canine recombinant IFNγ, Kingfisher, St. Paul, MN) or TNFα (50 ng/mL; canine recombinant TNFα, Kingfisher) or a dual stimulation with both IFNγ (50 ng/mL, Kingfisher) and TNFα (50 ng/mL, Kingfisher).

    Techniques: Derivative Assay, Activity Assay, Recombinant

    Relative gene expression after 96 h following AdMSC stimulation with interferon-gamma (IFN-γ). a Relative expression of BDNF, b GDNF, c HGF, and d IL-10. The samples were tested with two reference genes, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and hypoxanthine phosphoribosyl transferase (HPRT). Data are represented as mean ± SEM. p

    Journal: Stem Cell Research & Therapy

    Article Title: 3D-printed nerve guidance conduits multi-functionalized with canine multipotent mesenchymal stromal cells promote neuroregeneration after sciatic nerve injury in rats

    doi: 10.1186/s13287-021-02315-8

    Figure Lengend Snippet: Relative gene expression after 96 h following AdMSC stimulation with interferon-gamma (IFN-γ). a Relative expression of BDNF, b GDNF, c HGF, and d IL-10. The samples were tested with two reference genes, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and hypoxanthine phosphoribosyl transferase (HPRT). Data are represented as mean ± SEM. p

    Article Snippet: Subsequently, cells were stimulated with 0.75 mL basal medium containing IFN-γ (50 ng/mL, IFN- γ canine recombinant; Kingfisher Biotech, Saint Paul, USA) for 96 h. At this point, the cells were collected using TRIzol reagent (Invitrogen, São Paulo, Brazil) and stored at − 80 °C for RNA extraction and analysis of gene expression.

    Techniques: Expressing