recombinant bovine tnf α (Kingfisher Biotech)


Structured Review

Recombinant Bovine Tnf α, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant bovine tnf α/product/Kingfisher Biotech
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Dynamics of Progesterone, TNF-α, and a Metabolite of PGF2α in Blood Plasma of Beef Cows following Embryo Transfer"
Article Title: Dynamics of Progesterone, TNF-α, and a Metabolite of PGF2α in Blood Plasma of Beef Cows following Embryo Transfer
Journal: Veterinary Medicine International
doi: 10.1155/2014/650272

Figure Legend Snippet: Concentrations (Mean ± SEM) of TNF- α among treatment groups on d 7, d 14, and d 21. Treatment x day interaction ( P ≤ 0.05); CIDR = controlled internal drug release; GnRH = gonadotropin releasing hormone; hCG = human chorionic gonadotropin.
Techniques Used:

Figure Legend Snippet: Concentrations (Mean ± SEM) of TNF- α in pregnant and nonpregnant cows on d 7, d 14, and d 21. Pregnancy status x day interaction ( P ≤ 0.05); CIDR = controlled internal drug release; GnRH = gonadotropin releasing hormone; hCG = human chorionic gonadotropin.
Techniques Used:
2) Product Images from "Prostaglandin E2 Induction Suppresses the Th1 Immune Responses in Cattle with Johne's Disease"
Article Title: Prostaglandin E2 Induction Suppresses the Th1 Immune Responses in Cattle with Johne's Disease
Journal: Infection and Immunity
doi: 10.1128/IAI.00910-17
![... assayed by flow cytometry (a). IFN-γ (b) and TNF-α (c) production was determined by ELISA in duplicate. ... Functional analysis of the COX-2 inhibitor. PBMCs from uninfected cattle ( n = 9 [a and b] or 8 [c]) were cultured with meloxicam in the presence of anti-CD3 and anti-CD28 MAbs. CD8 + cell proliferation was assayed by flow cytometry (a). IFN-γ (b) and TNF-α (c) production was determined by ELISA in duplicate. Statistical significance was determined by the Wilcoxon signed-rank test.](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5913856/bin/zii9990923900002.jpg)
Figure Legend Snippet: Functional analysis of the COX-2 inhibitor. PBMCs from uninfected cattle ( n = 9 [a and b] or 8 [c]) were cultured with meloxicam in the presence of anti-CD3 and anti-CD28 MAbs. CD8 + cell proliferation was assayed by flow cytometry (a). IFN-γ (b) and TNF-α (c) production was determined by ELISA in duplicate. Statistical significance was determined by the Wilcoxon signed-rank test.
Techniques Used: Functional Assay, Cell Culture, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay
![... was assayed by flow cytometry. IFN-γ (c) and TNF-α (d) production was determined by ELISA. (e to ... Immunosuppressive effects of PGE 2 . (a to d) PBMCs from uninfected cattle ( n = 6 [a to c] or 8 [d]) were cultured with PGE 2 in the presence of anti-CD3 and anti-CD28 MAbs for 72 h. The proliferation of CD4 + cells (a) and CD8 + cells (b) was assayed by flow cytometry. IFN-γ (c) and TNF-α (d) production was determined by ELISA. (e to k) PBMCs from uninfected cattle ( n = 6 [e to i] or 7 [j and k]) were cultured with PGE 2 for 24 h. Real-time PCR was performed in duplicate to quantitate the mRNA expression of IFN-γ (e), IL-2 (f), TNF-α (g), IL-10 (h), STAT3 (i), and PD-L1 (j). The expression of PD-L1 protein was measured by flow cytometry (k). Statistical significance was determined by the Steel-Dwass test (a to c) or the Wilcoxon signed-rank test (d to k).](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5913856/bin/zii9990923900001.jpg)
Figure Legend Snippet: Immunosuppressive effects of PGE 2 . (a to d) PBMCs from uninfected cattle ( n = 6 [a to c] or 8 [d]) were cultured with PGE 2 in the presence of anti-CD3 and anti-CD28 MAbs for 72 h. The proliferation of CD4 + cells (a) and CD8 + cells (b) was assayed by flow cytometry. IFN-γ (c) and TNF-α (d) production was determined by ELISA. (e to k) PBMCs from uninfected cattle ( n = 6 [e to i] or 7 [j and k]) were cultured with PGE 2 for 24 h. Real-time PCR was performed in duplicate to quantitate the mRNA expression of IFN-γ (e), IL-2 (f), TNF-α (g), IL-10 (h), STAT3 (i), and PD-L1 (j). The expression of PD-L1 protein was measured by flow cytometry (k). Statistical significance was determined by the Steel-Dwass test (a to c) or the Wilcoxon signed-rank test (d to k).
Techniques Used: Cell Culture, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Expressing
![... was assayed by flow cytometry. IFN-γ (b) and TNF-α (c) production was determined by ELISA in duplicate. ... Activation of T-cell responses by the COX-2 inhibitor or anti-PD-L1 MAb. (a to c) PBMCs from cattle experimentally infected with M. avium subsp. paratuberculosis ( n = 7 [a and b] or 6 [c]) were cultured with meloxicam in the presence of J-PPD. CD8 + cell proliferation (a) was assayed by flow cytometry. IFN-γ (b) and TNF-α (c) production was determined by ELISA in duplicate. (d and e) PBMCs from the infected cattle ( n = 7) were cultured with anti-PD-L1 MAb in the presence of J-PPD. CD8 + cell proliferation (d) was assayed by flow cytometry. IFN-γ production (e) was determined by ELISA in duplicate. Statistical significance was determined by the Wilcoxon signed-rank test. Cont Ig, rat control IgG.](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5913856/bin/zii9990923900007.jpg)
Figure Legend Snippet: Activation of T-cell responses by the COX-2 inhibitor or anti-PD-L1 MAb. (a to c) PBMCs from cattle experimentally infected with M. avium subsp. paratuberculosis ( n = 7 [a and b] or 6 [c]) were cultured with meloxicam in the presence of J-PPD. CD8 + cell proliferation (a) was assayed by flow cytometry. IFN-γ (b) and TNF-α (c) production was determined by ELISA in duplicate. (d and e) PBMCs from the infected cattle ( n = 7) were cultured with anti-PD-L1 MAb in the presence of J-PPD. CD8 + cell proliferation (d) was assayed by flow cytometry. IFN-γ production (e) was determined by ELISA in duplicate. Statistical significance was determined by the Wilcoxon signed-rank test. Cont Ig, rat control IgG.
Techniques Used: Activation Assay, Infection, Cell Culture, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay