recombinant bovine tnf α  (Kingfisher Biotech)

Journal: Veterinary Medicine International

Article Title: Dynamics of Progesterone, TNF-α, and a Metabolite of PGF2α in Blood Plasma of Beef Cows following Embryo Transfer

doi: 10.1155/2014/650272

Figure Lengend Snippet: Concentrations (Mean ± SEM) of TNF- α among treatment groups on d 7, d 14, and d 21. Treatment x day interaction ( P ≤ 0.05); CIDR = controlled internal drug release; GnRH = gonadotropin releasing hormone; hCG = human chorionic gonadotropin.

Article Snippet: Antibody (rabbit anti-bovine TNF-α R7-93) generated against recombinant bovine TNF-α (kindly donated by Ciba-Geigy, Basel, Switzerland) was used as the primary antibody at a final tube dilution of 1 : 120,000 and recombinant bovine TNF-α (Kingfisher Biotech, St. Paul, MN) was radioiodinated and used as the assay tracer.

Techniques:

Journal: Veterinary Medicine International

Article Title: Dynamics of Progesterone, TNF-α, and a Metabolite of PGF2α in Blood Plasma of Beef Cows following Embryo Transfer

doi: 10.1155/2014/650272

Figure Lengend Snippet: Concentrations (Mean ± SEM) of TNF- α in pregnant and nonpregnant cows on d 7, d 14, and d 21. Pregnancy status x day interaction ( P ≤ 0.05); CIDR = controlled internal drug release; GnRH = gonadotropin releasing hormone; hCG = human chorionic gonadotropin.

Article Snippet: Antibody (rabbit anti-bovine TNF-α R7-93) generated against recombinant bovine TNF-α (kindly donated by Ciba-Geigy, Basel, Switzerland) was used as the primary antibody at a final tube dilution of 1 : 120,000 and recombinant bovine TNF-α (Kingfisher Biotech, St. Paul, MN) was radioiodinated and used as the assay tracer.

Techniques:

Journal: Infection and Immunity

Article Title: Prostaglandin E2 Induction Suppresses the Th1 Immune Responses in Cattle with Johne's Disease

doi: 10.1128/IAI.00910-17

Figure Lengend Snippet: Functional analysis of the COX-2 inhibitor. PBMCs from uninfected cattle ( n = 9 [a and b] or 8 [c]) were cultured with meloxicam in the presence of anti-CD3 and anti-CD28 MAbs. CD8 + cell proliferation was assayed by flow cytometry (a). IFN-γ (b) and TNF-α (c) production was determined by ELISA in duplicate. Statistical significance was determined by the Wilcoxon signed-rank test.

Article Snippet: To demonstrate the effects of PGE2 on IFN-γ and TNF-α production, 72-h culture supernatants of CFSE-labeled PBMCs were collected, and IFN-γ and TNF-α were determined by enzyme-linked immunosorbent assay (ELISA) for bovine IFN-γ (Mabtech, Nacka Strand, Sweden) and bovine TNF-α (Kingfisher Biotech, St. Paul, MN, USA), following the manufacturer's instructions.

Techniques: Functional Assay, Cell Culture, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Infection and Immunity

Article Title: Prostaglandin E2 Induction Suppresses the Th1 Immune Responses in Cattle with Johne's Disease

doi: 10.1128/IAI.00910-17

Figure Lengend Snippet: Immunosuppressive effects of PGE 2 . (a to d) PBMCs from uninfected cattle ( n = 6 [a to c] or 8 [d]) were cultured with PGE 2 in the presence of anti-CD3 and anti-CD28 MAbs for 72 h. The proliferation of CD4 + cells (a) and CD8 + cells (b) was assayed by flow cytometry. IFN-γ (c) and TNF-α (d) production was determined by ELISA. (e to k) PBMCs from uninfected cattle ( n = 6 [e to i] or 7 [j and k]) were cultured with PGE 2 for 24 h. Real-time PCR was performed in duplicate to quantitate the mRNA expression of IFN-γ (e), IL-2 (f), TNF-α (g), IL-10 (h), STAT3 (i), and PD-L1 (j). The expression of PD-L1 protein was measured by flow cytometry (k). Statistical significance was determined by the Steel-Dwass test (a to c) or the Wilcoxon signed-rank test (d to k).

Article Snippet: To demonstrate the effects of PGE2 on IFN-γ and TNF-α production, 72-h culture supernatants of CFSE-labeled PBMCs were collected, and IFN-γ and TNF-α were determined by enzyme-linked immunosorbent assay (ELISA) for bovine IFN-γ (Mabtech, Nacka Strand, Sweden) and bovine TNF-α (Kingfisher Biotech, St. Paul, MN, USA), following the manufacturer's instructions.

Techniques: Cell Culture, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Expressing

Journal: Infection and Immunity

Article Title: Prostaglandin E2 Induction Suppresses the Th1 Immune Responses in Cattle with Johne's Disease

doi: 10.1128/IAI.00910-17

Figure Lengend Snippet: Activation of T-cell responses by the COX-2 inhibitor or anti-PD-L1 MAb. (a to c) PBMCs from cattle experimentally infected with M. avium subsp. paratuberculosis ( n = 7 [a and b] or 6 [c]) were cultured with meloxicam in the presence of J-PPD. CD8 + cell proliferation (a) was assayed by flow cytometry. IFN-γ (b) and TNF-α (c) production was determined by ELISA in duplicate. (d and e) PBMCs from the infected cattle ( n = 7) were cultured with anti-PD-L1 MAb in the presence of J-PPD. CD8 + cell proliferation (d) was assayed by flow cytometry. IFN-γ production (e) was determined by ELISA in duplicate. Statistical significance was determined by the Wilcoxon signed-rank test. Cont Ig, rat control IgG.

Article Snippet: To demonstrate the effects of PGE2 on IFN-γ and TNF-α production, 72-h culture supernatants of CFSE-labeled PBMCs were collected, and IFN-γ and TNF-α were determined by enzyme-linked immunosorbent assay (ELISA) for bovine IFN-γ (Mabtech, Nacka Strand, Sweden) and bovine TNF-α (Kingfisher Biotech, St. Paul, MN, USA), following the manufacturer's instructions.

Techniques: Activation Assay, Infection, Cell Culture, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay