recombinant bovine il 2  (Kingfisher Biotech)


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  • 90
    Name:
    Recombinant Bovine IL 2
    Description:

    Catalog Number:
    RP0026B-100
    Price:
    750.0
    Source:
    Yeast
    Purity:
    98%
    Quantity:
    100 ug
    Molecular Weight:
    15.5 kDa
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    Structured Review

    Kingfisher Biotech recombinant bovine il 2
    Inhibition of IFN‐γ and TNF‐α production from CD4 + T cells by TGF‐β. PBMCs from normal cattle (IFN‐γ: n = 12, TNF‐α: n = 9) and BLV‐infected cattle ( n = 11) were pretreated with TGF‐β for 2 h and were cultivated with <t>IL‐2</t> alone (A and B) or synthesized peptides from the BLV envelop region (C and D). IFN‐γ‐ or TNF‐α‐producing CD4 + T cells were detected using flow cytometry.

    https://www.bioz.com/result/recombinant bovine il 2/product/Kingfisher Biotech
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant bovine il 2 - by Bioz Stars, 2021-09
    90/100 stars

    Images

    1) Product Images from "Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells"

    Article Title: Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells

    Journal: Immunity, Inflammation and Disease

    doi: 10.1002/iid3.93

    Inhibition of IFN‐γ and TNF‐α production from CD4 + T cells by TGF‐β. PBMCs from normal cattle (IFN‐γ: n = 12, TNF‐α: n = 9) and BLV‐infected cattle ( n = 11) were pretreated with TGF‐β for 2 h and were cultivated with IL‐2 alone (A and B) or synthesized peptides from the BLV envelop region (C and D). IFN‐γ‐ or TNF‐α‐producing CD4 + T cells were detected using flow cytometry.
    Figure Legend Snippet: Inhibition of IFN‐γ and TNF‐α production from CD4 + T cells by TGF‐β. PBMCs from normal cattle (IFN‐γ: n = 12, TNF‐α: n = 9) and BLV‐infected cattle ( n = 11) were pretreated with TGF‐β for 2 h and were cultivated with IL‐2 alone (A and B) or synthesized peptides from the BLV envelop region (C and D). IFN‐γ‐ or TNF‐α‐producing CD4 + T cells were detected using flow cytometry.

    Techniques Used: Inhibition, Infection, Synthesized, Flow Cytometry, Cytometry

    2) Product Images from "Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells"

    Article Title: Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells

    Journal: Immunity, Inflammation and Disease

    doi: 10.1002/iid3.93

    Inhibition of IFN‐γ and TNF‐α production from CD4 + T cells by TGF‐β. PBMCs from normal cattle (IFN‐γ: n = 12, TNF‐α: n = 9) and BLV‐infected cattle ( n = 11) were pretreated with TGF‐β for 2 h and were cultivated with IL‐2 alone (A and B) or synthesized peptides from the BLV envelop region (C and D). IFN‐γ‐ or TNF‐α‐producing CD4 + T cells were detected using flow cytometry.
    Figure Legend Snippet: Inhibition of IFN‐γ and TNF‐α production from CD4 + T cells by TGF‐β. PBMCs from normal cattle (IFN‐γ: n = 12, TNF‐α: n = 9) and BLV‐infected cattle ( n = 11) were pretreated with TGF‐β for 2 h and were cultivated with IL‐2 alone (A and B) or synthesized peptides from the BLV envelop region (C and D). IFN‐γ‐ or TNF‐α‐producing CD4 + T cells were detected using flow cytometry.

    Techniques Used: Inhibition, Infection, Synthesized, Flow Cytometry, Cytometry

    3) Product Images from "Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells"

    Article Title: Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells

    Journal: Immunity, Inflammation and Disease

    doi: 10.1002/iid3.93

    Inhibition of IFN‐γ and TNF‐α production from CD4 + T cells by TGF‐β. PBMCs from normal cattle (IFN‐γ: n = 12, TNF‐α: n = 9) and BLV‐infected cattle ( n = 11) were pretreated with TGF‐β for 2 h and were cultivated with IL‐2 alone (A and B) or synthesized peptides from the BLV envelop region (C and D). IFN‐γ‐ or TNF‐α‐producing CD4 + T cells were detected using flow cytometry.
    Figure Legend Snippet: Inhibition of IFN‐γ and TNF‐α production from CD4 + T cells by TGF‐β. PBMCs from normal cattle (IFN‐γ: n = 12, TNF‐α: n = 9) and BLV‐infected cattle ( n = 11) were pretreated with TGF‐β for 2 h and were cultivated with IL‐2 alone (A and B) or synthesized peptides from the BLV envelop region (C and D). IFN‐γ‐ or TNF‐α‐producing CD4 + T cells were detected using flow cytometry.

    Techniques Used: Inhibition, Infection, Synthesized, Flow Cytometry, Cytometry

    4) Product Images from "Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells"

    Article Title: Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells

    Journal: Immunity, Inflammation and Disease

    doi: 10.1002/iid3.93

    Inhibition of IFN‐γ and TNF‐α production from CD4 + T cells by TGF‐β. PBMCs from normal cattle (IFN‐γ: n = 12, TNF‐α: n = 9) and BLV‐infected cattle ( n = 11) were pretreated with TGF‐β for 2 h and were cultivated with IL‐2 alone (A and B) or synthesized peptides from the BLV envelop region (C and D). IFN‐γ‐ or TNF‐α‐producing CD4 + T cells were detected using flow cytometry.
    Figure Legend Snippet: Inhibition of IFN‐γ and TNF‐α production from CD4 + T cells by TGF‐β. PBMCs from normal cattle (IFN‐γ: n = 12, TNF‐α: n = 9) and BLV‐infected cattle ( n = 11) were pretreated with TGF‐β for 2 h and were cultivated with IL‐2 alone (A and B) or synthesized peptides from the BLV envelop region (C and D). IFN‐γ‐ or TNF‐α‐producing CD4 + T cells were detected using flow cytometry.

    Techniques Used: Inhibition, Infection, Synthesized, Flow Cytometry, Cytometry

    5) Product Images from "Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells"

    Article Title: Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells

    Journal: Immunity, Inflammation and Disease

    doi: 10.1002/iid3.93

    Inhibition of IFN‐γ and TNF‐α production from CD4 + T cells by TGF‐β. PBMCs from normal cattle (IFN‐γ: n = 12, TNF‐α: n = 9) and BLV‐infected cattle ( n = 11) were pretreated with TGF‐β for 2 h and were cultivated with IL‐2 alone (A and B) or synthesized peptides from the BLV envelop region (C and D). IFN‐γ‐ or TNF‐α‐producing CD4 + T cells were detected using flow cytometry.
    Figure Legend Snippet: Inhibition of IFN‐γ and TNF‐α production from CD4 + T cells by TGF‐β. PBMCs from normal cattle (IFN‐γ: n = 12, TNF‐α: n = 9) and BLV‐infected cattle ( n = 11) were pretreated with TGF‐β for 2 h and were cultivated with IL‐2 alone (A and B) or synthesized peptides from the BLV envelop region (C and D). IFN‐γ‐ or TNF‐α‐producing CD4 + T cells were detected using flow cytometry.

    Techniques Used: Inhibition, Infection, Synthesized, Flow Cytometry, Cytometry

    6) Product Images from "The Chronic Stages of Bovine Fasciola hepatica Are Dominated by CD4 T-Cell Exhaustion"

    Article Title: The Chronic Stages of Bovine Fasciola hepatica Are Dominated by CD4 T-Cell Exhaustion

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2017.01002

    Anergic CD4 T-cells are suppressive toward autologous responsive counterparts. CD4 T-cells taken from animals’ 2 weeks of postinfection were cocultured with autologous CD4 T-cells isolated from the hepatic lymph node at postmortem. (A) Proliferation in response to LFH is still detectable in week 2 CD4 T-cells, as per previous findings; however, when anergic week 13 CD4 cells are added at a ratio of five cells per 1 week 2 responder cells, we see a strong suppression of proliferation. (B) A similar coculture was established and the effects of α-IL-10 (left graph) and exogenous IL-2 (right graph) was determined. In the presence of α-IL-10, there was significant reversal of the negative effects of anergic CD4 cells on their responsive counterparts; however, there was no positive effect of exogenous IL-2. (C) IFN-γ levels in LFH-stimulated cells untreated (isotype control) or treated with IL-2 and α-IL-10 were determined in the coculture system. In the presence of week 13 anergic CD4 cells, the IFN-γ response, from responsive week 2 cells, was suppressed while IL-2, but not α-IL-10 treatment, could abrogate the effect of week 13 CD4 T-cells on the responsive population. Bars represent the mean of seven animals’ ±SEM. Data in (A) and (B,C) were tested by Kruskal–Wallis with Dunn’s multiple comparisons and Friedman test with a post hoc comparison using a Wilcoxon ranked-sign test, respectively. ND, not detected; NS, not significant, * P
    Figure Legend Snippet: Anergic CD4 T-cells are suppressive toward autologous responsive counterparts. CD4 T-cells taken from animals’ 2 weeks of postinfection were cocultured with autologous CD4 T-cells isolated from the hepatic lymph node at postmortem. (A) Proliferation in response to LFH is still detectable in week 2 CD4 T-cells, as per previous findings; however, when anergic week 13 CD4 cells are added at a ratio of five cells per 1 week 2 responder cells, we see a strong suppression of proliferation. (B) A similar coculture was established and the effects of α-IL-10 (left graph) and exogenous IL-2 (right graph) was determined. In the presence of α-IL-10, there was significant reversal of the negative effects of anergic CD4 cells on their responsive counterparts; however, there was no positive effect of exogenous IL-2. (C) IFN-γ levels in LFH-stimulated cells untreated (isotype control) or treated with IL-2 and α-IL-10 were determined in the coculture system. In the presence of week 13 anergic CD4 cells, the IFN-γ response, from responsive week 2 cells, was suppressed while IL-2, but not α-IL-10 treatment, could abrogate the effect of week 13 CD4 T-cells on the responsive population. Bars represent the mean of seven animals’ ±SEM. Data in (A) and (B,C) were tested by Kruskal–Wallis with Dunn’s multiple comparisons and Friedman test with a post hoc comparison using a Wilcoxon ranked-sign test, respectively. ND, not detected; NS, not significant, * P

    Techniques Used: Isolation

    Failure to produce IL-2 and markers of anergy are coincident with non-responsive CD4 T-cells. (A) CD4 T-cells were isolated from the hepatic lymph node (HLN) of naïve or infected animals and cultured with α-CD3, LFH, or PBS (nil) for 72 h. Supernatants were recovered at every 24 h and tested for IL-2. There is a clear failure of CD4 T-cells from the HLNs of infected animals to secrete IL-2. However, naïve CD4 T-cells readily secrete IL-2 over time with a near linear accumulation of cytokine. (B) mRNA from CD4 T-cells was isolated, cDNA prepared, and qPCR carried out. There is a clear increase in pdcd1 expression from week 2 onward when compared to naïve animals. Ctla4 expression is significantly upregulated at week 13 but not week 2. (C) The effect of exogenous IL-2, or neutralizing IL-10 or TGF-β antibody on the expression of IFN-γ (left) and IL-13 (right) in infected CD4 T-cells from HLN was examined. There was a clear impact of IL-2 on IFN-γ, and IL-13 production was restored in the presence of all three treatments but to varying degrees. Ctrl represents cells treated with isotype Ab only at equal concentrations to neutralizing Ab. (D) CD4 T-cell proliferative responses in the HLN were assessed using the MTT dye assay after stimulation in the presence of treatments as in (C) . LFH and α-CD3 responsiveness was restored in the presence of α-IL-10 when compared within stimulation to non-treated controls. Bars represent the mean of seven animals ±SEM. Significant differences were determined using Friedman test with a post hoc comparison using a Wilcoxon ranked-sign test (A,C,D) and Kruskal–Wallis with Dunn’s multiple comparisons (B) where * P
    Figure Legend Snippet: Failure to produce IL-2 and markers of anergy are coincident with non-responsive CD4 T-cells. (A) CD4 T-cells were isolated from the hepatic lymph node (HLN) of naïve or infected animals and cultured with α-CD3, LFH, or PBS (nil) for 72 h. Supernatants were recovered at every 24 h and tested for IL-2. There is a clear failure of CD4 T-cells from the HLNs of infected animals to secrete IL-2. However, naïve CD4 T-cells readily secrete IL-2 over time with a near linear accumulation of cytokine. (B) mRNA from CD4 T-cells was isolated, cDNA prepared, and qPCR carried out. There is a clear increase in pdcd1 expression from week 2 onward when compared to naïve animals. Ctla4 expression is significantly upregulated at week 13 but not week 2. (C) The effect of exogenous IL-2, or neutralizing IL-10 or TGF-β antibody on the expression of IFN-γ (left) and IL-13 (right) in infected CD4 T-cells from HLN was examined. There was a clear impact of IL-2 on IFN-γ, and IL-13 production was restored in the presence of all three treatments but to varying degrees. Ctrl represents cells treated with isotype Ab only at equal concentrations to neutralizing Ab. (D) CD4 T-cell proliferative responses in the HLN were assessed using the MTT dye assay after stimulation in the presence of treatments as in (C) . LFH and α-CD3 responsiveness was restored in the presence of α-IL-10 when compared within stimulation to non-treated controls. Bars represent the mean of seven animals ±SEM. Significant differences were determined using Friedman test with a post hoc comparison using a Wilcoxon ranked-sign test (A,C,D) and Kruskal–Wallis with Dunn’s multiple comparisons (B) where * P

    Techniques Used: Isolation, Infection, Cell Culture, Real-time Polymerase Chain Reaction, Expressing, MTT Assay

    Related Articles

    Cell Culture:

    Article Title: Regulatory T Cell Activity and Signs of T Cell Unresponsiveness in Bovine Paratuberculosis
    Article Snippet: .. CD4+ CD25+ T cell enrichment The 3 × 105 CD4+ CD25+ lymphocytes were cultured with 4-day-old MAP-infected MDMs in RPMI complete media plus enrichment cocktail [1 nM rapamycin (Sigma-Aldrich catalog #R03950-1MG), 5 ng/mL recombinant bovine IL2 (Kingfisher Biotech, Inc. catalog #RP0026B-005), and 2.5 ng/mL purified human transforming growth factor beta 1 (BD Biosciences catalog #354039)] for 8 days at 38°C and 5% CO2 , with enriched media being refreshed every 72 h. ..

    Article Title: Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells
    Article Snippet: .. To evaluate cytotoxic effects of TGF‐β, CD3‐ IgM‐ NKp46+ cells were cultured in the presence of recombinant bovine IL‐2 (10 ng/mL; Kingfisher Biotech) alone or with recombinant bovine TGF‐β (5 ng/mL; Genorise Scientific) for 40 h. Cytotoxicity (%) was then determined as above at a NK:P815 cell ratio of 2:1. ..

    Article Title: The Chronic Stages of Bovine Fasciola hepatica Are Dominated by CD4 T-Cell Exhaustion
    Article Snippet: .. Where indicated, anti-IL-10 (AbDserotec—clone CC320) ( , ) or anti-TGF (R & D Systems—polyclonal AB-100-NA) ( ) were used at 1 µg/mL, alternatively cells were cultured in the presence of recombinant bovine IL-2 (Kingfisher Biotech RP0026B at the indicated concentrations), all cultures were in RMPI-1640 with 10% heat-inactivated fetal calf serum (FCS), penicillin (100 U/mL), and streptomycin (100 µg/mL). ..

    Recombinant:

    Article Title: Regulatory T Cell Activity and Signs of T Cell Unresponsiveness in Bovine Paratuberculosis
    Article Snippet: .. CD4+ CD25+ T cell enrichment The 3 × 105 CD4+ CD25+ lymphocytes were cultured with 4-day-old MAP-infected MDMs in RPMI complete media plus enrichment cocktail [1 nM rapamycin (Sigma-Aldrich catalog #R03950-1MG), 5 ng/mL recombinant bovine IL2 (Kingfisher Biotech, Inc. catalog #RP0026B-005), and 2.5 ng/mL purified human transforming growth factor beta 1 (BD Biosciences catalog #354039)] for 8 days at 38°C and 5% CO2 , with enriched media being refreshed every 72 h. ..

    Article Title: Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells
    Article Snippet: .. Purified NK cells (more than 98% purity) were cultivated in the presence of recombinant bovine IL‐2 (10 ng/mL; Kingfisher Biotech) for 16 h. Target P815 cells were pretreated with 0.1% bovine serum albumin (BSA) in PBS containing 0.2 µM carboxyfluorescein diacetate succinimidyl ester (CFSE)(Life technologies) for 15 min at room temperature. ..

    Article Title: Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells
    Article Snippet: .. To evaluate cytotoxic effects of TGF‐β, CD3‐ IgM‐ NKp46+ cells were cultured in the presence of recombinant bovine IL‐2 (10 ng/mL; Kingfisher Biotech) alone or with recombinant bovine TGF‐β (5 ng/mL; Genorise Scientific) for 40 h. Cytotoxicity (%) was then determined as above at a NK:P815 cell ratio of 2:1. ..

    Article Title: Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells
    Article Snippet: .. T‐cell bioassay with bovine recombinant TGF‐β PBMCs were pre‐cultured for 2 h in the presence of recombinant bovine TGF‐β (1 ng/mL; Genorise Scientific, Glen Mills, PA, USA) and were then incubated with mouse anti‐bovine CD3 antibody (2 µg/mL; VMRD), mouse anti‐bovine CD28 antibody (2 µg/mL; AbD serotec), and recombinant bovine IL‐2 (10 ng/mL; Kingfisher Biotech) alone or with BLV‐gp51 peptide mix (5 µg/mL) for 24 h. Brefeldin A (10 µg/mL; Sigma‐Aldrich) was added to the medium 6 h before cell harvest, and IFN‐γ and TNF‐α were determined in CD4+ cells using flow cytometry as described above. ..

    Article Title: Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells
    Article Snippet: .. Cytokine assay Bovine whole blood was cultivated in the presence of mouse anti‐bovine CD3 antibody (2 µg/mL; MM1A: VMRD, Pullman, WA), mouse anti‐bovine CD28 antibody (2 µg/mL; AbD Serotec, Oxford, UK), and recombinant bovine IL‐2 (10 ng/mL; Kingfisher Biotech, Saint Paul, MN) in 12‐well plates. ..

    Article Title: The Chronic Stages of Bovine Fasciola hepatica Are Dominated by CD4 T-Cell Exhaustion
    Article Snippet: .. Where indicated, anti-IL-10 (AbDserotec—clone CC320) ( , ) or anti-TGF (R & D Systems—polyclonal AB-100-NA) ( ) were used at 1 µg/mL, alternatively cells were cultured in the presence of recombinant bovine IL-2 (Kingfisher Biotech RP0026B at the indicated concentrations), all cultures were in RMPI-1640 with 10% heat-inactivated fetal calf serum (FCS), penicillin (100 U/mL), and streptomycin (100 µg/mL). ..

    Article Title: Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells
    Article Snippet: .. To determine IFN‐γ production by NK cells, PBMCs were cultivated in the presence of recombinant bovine IL‐2 (10 ng/mL; Kingfisher Biotech) and recombinant human IL‐12 (400 pg/mL; eBioscience) for 72 h. Brefeldin A (10 µg/mL; Sigma‐Aldrich) was then added to the medium 6 h before cell harvest. ..

    Purification:

    Article Title: Regulatory T Cell Activity and Signs of T Cell Unresponsiveness in Bovine Paratuberculosis
    Article Snippet: .. CD4+ CD25+ T cell enrichment The 3 × 105 CD4+ CD25+ lymphocytes were cultured with 4-day-old MAP-infected MDMs in RPMI complete media plus enrichment cocktail [1 nM rapamycin (Sigma-Aldrich catalog #R03950-1MG), 5 ng/mL recombinant bovine IL2 (Kingfisher Biotech, Inc. catalog #RP0026B-005), and 2.5 ng/mL purified human transforming growth factor beta 1 (BD Biosciences catalog #354039)] for 8 days at 38°C and 5% CO2 , with enriched media being refreshed every 72 h. ..

    Article Title: Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells
    Article Snippet: .. Purified NK cells (more than 98% purity) were cultivated in the presence of recombinant bovine IL‐2 (10 ng/mL; Kingfisher Biotech) for 16 h. Target P815 cells were pretreated with 0.1% bovine serum albumin (BSA) in PBS containing 0.2 µM carboxyfluorescein diacetate succinimidyl ester (CFSE)(Life technologies) for 15 min at room temperature. ..

    Incubation:

    Article Title: Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells
    Article Snippet: .. T‐cell bioassay with bovine recombinant TGF‐β PBMCs were pre‐cultured for 2 h in the presence of recombinant bovine TGF‐β (1 ng/mL; Genorise Scientific, Glen Mills, PA, USA) and were then incubated with mouse anti‐bovine CD3 antibody (2 µg/mL; VMRD), mouse anti‐bovine CD28 antibody (2 µg/mL; AbD serotec), and recombinant bovine IL‐2 (10 ng/mL; Kingfisher Biotech) alone or with BLV‐gp51 peptide mix (5 µg/mL) for 24 h. Brefeldin A (10 µg/mL; Sigma‐Aldrich) was added to the medium 6 h before cell harvest, and IFN‐γ and TNF‐α were determined in CD4+ cells using flow cytometry as described above. ..

    Flow Cytometry:

    Article Title: Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells
    Article Snippet: .. T‐cell bioassay with bovine recombinant TGF‐β PBMCs were pre‐cultured for 2 h in the presence of recombinant bovine TGF‐β (1 ng/mL; Genorise Scientific, Glen Mills, PA, USA) and were then incubated with mouse anti‐bovine CD3 antibody (2 µg/mL; VMRD), mouse anti‐bovine CD28 antibody (2 µg/mL; AbD serotec), and recombinant bovine IL‐2 (10 ng/mL; Kingfisher Biotech) alone or with BLV‐gp51 peptide mix (5 µg/mL) for 24 h. Brefeldin A (10 µg/mL; Sigma‐Aldrich) was added to the medium 6 h before cell harvest, and IFN‐γ and TNF‐α were determined in CD4+ cells using flow cytometry as described above. ..

    Cytometry:

    Article Title: Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells
    Article Snippet: .. T‐cell bioassay with bovine recombinant TGF‐β PBMCs were pre‐cultured for 2 h in the presence of recombinant bovine TGF‐β (1 ng/mL; Genorise Scientific, Glen Mills, PA, USA) and were then incubated with mouse anti‐bovine CD3 antibody (2 µg/mL; VMRD), mouse anti‐bovine CD28 antibody (2 µg/mL; AbD serotec), and recombinant bovine IL‐2 (10 ng/mL; Kingfisher Biotech) alone or with BLV‐gp51 peptide mix (5 µg/mL) for 24 h. Brefeldin A (10 µg/mL; Sigma‐Aldrich) was added to the medium 6 h before cell harvest, and IFN‐γ and TNF‐α were determined in CD4+ cells using flow cytometry as described above. ..

    Cytokine Assay:

    Article Title: Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells
    Article Snippet: .. Cytokine assay Bovine whole blood was cultivated in the presence of mouse anti‐bovine CD3 antibody (2 µg/mL; MM1A: VMRD, Pullman, WA), mouse anti‐bovine CD28 antibody (2 µg/mL; AbD Serotec, Oxford, UK), and recombinant bovine IL‐2 (10 ng/mL; Kingfisher Biotech, Saint Paul, MN) in 12‐well plates. ..

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  • 90
    Kingfisher Biotech recombinant bovine il 2
    Inhibition of IFN‐γ and TNF‐α production from CD4 + T cells by TGF‐β. PBMCs from normal cattle (IFN‐γ: n = 12, TNF‐α: n = 9) and BLV‐infected cattle ( n = 11) were pretreated with TGF‐β for 2 h and were cultivated with <t>IL‐2</t> alone (A and B) or synthesized peptides from the BLV envelop region (C and D). IFN‐γ‐ or TNF‐α‐producing CD4 + T cells were detected using flow cytometry.
    Recombinant Bovine Il 2, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant bovine il 2/product/Kingfisher Biotech
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant bovine il 2 - by Bioz Stars, 2021-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    Inhibition of IFN‐γ and TNF‐α production from CD4 + T cells by TGF‐β. PBMCs from normal cattle (IFN‐γ: n = 12, TNF‐α: n = 9) and BLV‐infected cattle ( n = 11) were pretreated with TGF‐β for 2 h and were cultivated with IL‐2 alone (A and B) or synthesized peptides from the BLV envelop region (C and D). IFN‐γ‐ or TNF‐α‐producing CD4 + T cells were detected using flow cytometry.

    Journal: Immunity, Inflammation and Disease

    Article Title: Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells

    doi: 10.1002/iid3.93

    Figure Lengend Snippet: Inhibition of IFN‐γ and TNF‐α production from CD4 + T cells by TGF‐β. PBMCs from normal cattle (IFN‐γ: n = 12, TNF‐α: n = 9) and BLV‐infected cattle ( n = 11) were pretreated with TGF‐β for 2 h and were cultivated with IL‐2 alone (A and B) or synthesized peptides from the BLV envelop region (C and D). IFN‐γ‐ or TNF‐α‐producing CD4 + T cells were detected using flow cytometry.

    Article Snippet: To evaluate cytotoxic effects of TGF‐β, CD3‐ IgM‐ NKp46+ cells were cultured in the presence of recombinant bovine IL‐2 (10 ng/mL; Kingfisher Biotech) alone or with recombinant bovine TGF‐β (5 ng/mL; Genorise Scientific) for 40 h. Cytotoxicity (%) was then determined as above at a NK:P815 cell ratio of 2:1.

    Techniques: Inhibition, Infection, Synthesized, Flow Cytometry, Cytometry