recombinant bovine il 4  (Kingfisher Biotech)


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    Name:
    Recombinant Bovine IL 4
    Description:

    Catalog Number:
    RP0001B-100
    Price:
    750.0
    Source:
    Yeast
    Purity:
    98%
    Quantity:
    100 ug
    Molecular Weight:
    12.6 kDa
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    Structured Review

    Kingfisher Biotech recombinant bovine il 4
    IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or <t>IL-4</t> (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown

    https://www.bioz.com/result/recombinant bovine il 4/product/Kingfisher Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant bovine il 4 - by Bioz Stars, 2021-09
    93/100 stars

    Images

    1) Product Images from "Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity"

    Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

    Journal: BMC Veterinary Research

    doi: 10.1186/s12917-019-1785-0

    IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or IL-4 (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown
    Figure Legend Snippet: IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or IL-4 (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown

    Techniques Used: Concentration Assay

    Fold changes in MDM production of nos2 ( a ) and arg1 ( b ) following stimulation with LPS (1000 ng/ml) IFNγ (20 ng/ml) or IL-4 (20 ng/ml) IL-13 (20 ng/ml) compared with changes in control cells, normalised with reference to transcription levels of GAPDH at 12 24 h. Fold changes in levels of nitric oxide in MDM cell supernatants from stimulated cells in comparison to unstimulated controls are shown in c . Supernatants and mRNA were harvested contemporaneously from the same cell populations and were performed in triplicate. Data from the same representative individual are shown in a , b and c
    Figure Legend Snippet: Fold changes in MDM production of nos2 ( a ) and arg1 ( b ) following stimulation with LPS (1000 ng/ml) IFNγ (20 ng/ml) or IL-4 (20 ng/ml) IL-13 (20 ng/ml) compared with changes in control cells, normalised with reference to transcription levels of GAPDH at 12 24 h. Fold changes in levels of nitric oxide in MDM cell supernatants from stimulated cells in comparison to unstimulated controls are shown in c . Supernatants and mRNA were harvested contemporaneously from the same cell populations and were performed in triplicate. Data from the same representative individual are shown in a , b and c

    Techniques Used:

    Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h
    Figure Legend Snippet: Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h

    Techniques Used: Derivative Assay

    2) Product Images from "Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity"

    Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

    Journal: BMC Veterinary Research

    doi: 10.1186/s12917-019-1785-0

    IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or IL-4 (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown
    Figure Legend Snippet: IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or IL-4 (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown

    Techniques Used: Concentration Assay

    Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h
    Figure Legend Snippet: Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h

    Techniques Used: Derivative Assay

    3) Product Images from "Generating Bovine Monocyte-Derived Dendritic Cells for Experimental and Clinical Applications Using Commercially Available Serum-Free Medium"

    Article Title: Generating Bovine Monocyte-Derived Dendritic Cells for Experimental and Clinical Applications Using Commercially Available Serum-Free Medium

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2020.591185

    The percent positive cells or MdFI of CD14 (A) , CD86 (B) , and CD205 (C , D) expressed by bovine MoDC generated from enriched monocytes via MACS and plate adherence (MACS+Adh) and cultured in AIM-V serum-free medium (AV-SF) for 4 days. MoDC were differentiated using various concentrations of recombinant bovine IL-4 (rbIL-4) and recombinant bovine granulocyte-macrophage colony-stimulating factor (rbGM-CSF). Each circle represents that data point for each individual animal (n = 3) used for this experiment. Horizontal lines represent the mean ± SEM for that condition. *P
    Figure Legend Snippet: The percent positive cells or MdFI of CD14 (A) , CD86 (B) , and CD205 (C , D) expressed by bovine MoDC generated from enriched monocytes via MACS and plate adherence (MACS+Adh) and cultured in AIM-V serum-free medium (AV-SF) for 4 days. MoDC were differentiated using various concentrations of recombinant bovine IL-4 (rbIL-4) and recombinant bovine granulocyte-macrophage colony-stimulating factor (rbGM-CSF). Each circle represents that data point for each individual animal (n = 3) used for this experiment. Horizontal lines represent the mean ± SEM for that condition. *P

    Techniques Used: Generated, Magnetic Cell Separation, Cell Culture, Recombinant

    4) Product Images from "Bovine WC1+ and WC1neg γδ T Lymphocytes Influence Monocyte Differentiation and Monocyte-Derived Dendritic Cell Maturation during In Vitro Mycobacterium avium Subspecies paratuberculosis Infection"

    Article Title: Bovine WC1+ and WC1neg γδ T Lymphocytes Influence Monocyte Differentiation and Monocyte-Derived Dendritic Cell Maturation during In Vitro Mycobacterium avium Subspecies paratuberculosis Infection

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2017.00534

    Dendritic cell maturation assays . Immature monocyte-derived dendritic cells (iMDDCs) were generated by adding IL-4 and GM-CSF to fresh monocyte cultures. After 6 days of differentiation, 1 × 10 6 sorted γδ T lymphocytes were added to wells containing 2 × 10 5 iMDDCs for 48 h (iMDDC + WC1 + and iMDDC + WC1 neg ). On day 6, live Mycobacterium avium subspecies paratuberculosis was added at a multiplicity of infection of 10:1 to evaluate if it affected iMDDC maturation after 48 h.
    Figure Legend Snippet: Dendritic cell maturation assays . Immature monocyte-derived dendritic cells (iMDDCs) were generated by adding IL-4 and GM-CSF to fresh monocyte cultures. After 6 days of differentiation, 1 × 10 6 sorted γδ T lymphocytes were added to wells containing 2 × 10 5 iMDDCs for 48 h (iMDDC + WC1 + and iMDDC + WC1 neg ). On day 6, live Mycobacterium avium subspecies paratuberculosis was added at a multiplicity of infection of 10:1 to evaluate if it affected iMDDC maturation after 48 h.

    Techniques Used: Derivative Assay, Generated, Infection

    Related Articles

    Recombinant:

    Article Title: Generating Bovine Monocyte-Derived Dendritic Cells for Experimental and Clinical Applications Using Commercially Available Serum-Free Medium
    Article Snippet: .. The adhered, enriched monocytes were then supplemented with fresh medium containing 250 ng/ml recombinant bovine interleukin 4 (rbIL-4) and 25 ng/ml recombinant bovine granulocyte-macrophage colony-stimulating factor (rbGM-CSF) sourced from Kingfisher Biotech (Saint Paul, MN) and cells were incubated at 37°C and 5% CO2 . ..

    Article Title: Bovine WC1+ and WC1neg γδ T Lymphocytes Influence Monocyte Differentiation and Monocyte-Derived Dendritic Cell Maturation during In Vitro Mycobacterium avium Subspecies paratuberculosis Infection
    Article Snippet: .. To obtain iMDDCs, complete RPMI was supplemented with 200 ng/mL of recombinant bovine interleukin-4 (Kingfisher Biotech, MN, USA) and 100 ng/mL of recombinant bovine GM-CSF (Kingfisher Biotech). ..

    Article Title: Efficacy of mucosal polyanhydride nanovaccine against respiratory syncytial virus infection in the neonatal calf
    Article Snippet: .. Recombinant bovine IL-4 and recombinant bovine GM-CSF were purchased from Kingfisher Biotech, Inc. After 6 days, moDC were seeded at 5 × 105 cells per well in 24-well plates and incubated with 10 μg/mL ‘empty’ or BRSV-F/G loaded CPTEG:CPH particles. ..

    Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity
    Article Snippet: .. MDM or SM were stimulated by addition of 50–2000 ng/ml LPS from E. coli 0111:B4 (Insight Biotechnology Ltd., Middlesex) and/or 20-100 ng/ml bovine IFNγ (ThermoFisher Scientific, UK) or with 10-40 ng/ml recombinant bovine IL-4 (Kingfisher Biotech Inc., St Paul) and/or 10-20 ng/ml recombinant bovine IL-13 (Kingfisher Biotech Inc). ..

    Incubation:

    Article Title: Generating Bovine Monocyte-Derived Dendritic Cells for Experimental and Clinical Applications Using Commercially Available Serum-Free Medium
    Article Snippet: .. The adhered, enriched monocytes were then supplemented with fresh medium containing 250 ng/ml recombinant bovine interleukin 4 (rbIL-4) and 25 ng/ml recombinant bovine granulocyte-macrophage colony-stimulating factor (rbGM-CSF) sourced from Kingfisher Biotech (Saint Paul, MN) and cells were incubated at 37°C and 5% CO2 . ..

    Article Title: Efficacy of mucosal polyanhydride nanovaccine against respiratory syncytial virus infection in the neonatal calf
    Article Snippet: .. Recombinant bovine IL-4 and recombinant bovine GM-CSF were purchased from Kingfisher Biotech, Inc. After 6 days, moDC were seeded at 5 × 105 cells per well in 24-well plates and incubated with 10 μg/mL ‘empty’ or BRSV-F/G loaded CPTEG:CPH particles. ..

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  • 93
    Kingfisher Biotech recombinant bovine il 4
    IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or <t>IL-4</t> (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown
    Recombinant Bovine Il 4, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant bovine il 4/product/Kingfisher Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant bovine il 4 - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or IL-4 (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown

    Journal: BMC Veterinary Research

    Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

    doi: 10.1186/s12917-019-1785-0

    Figure Lengend Snippet: IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or IL-4 (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown

    Article Snippet: Cell stimulation MDM or SM were stimulated by addition of 50–2000 ng/ml LPS from E. coli 0111:B4 (Insight Biotechnology Ltd., Middlesex) and/or 20-100 ng/ml bovine IFNγ (ThermoFisher Scientific, UK) or with 10-40 ng/ml recombinant bovine IL-4 (Kingfisher Biotech Inc., St Paul) and/or 10-20 ng/ml recombinant bovine IL-13 (Kingfisher Biotech Inc).

    Techniques: Concentration Assay

    Fold changes in MDM production of nos2 ( a ) and arg1 ( b ) following stimulation with LPS (1000 ng/ml) IFNγ (20 ng/ml) or IL-4 (20 ng/ml) IL-13 (20 ng/ml) compared with changes in control cells, normalised with reference to transcription levels of GAPDH at 12 24 h. Fold changes in levels of nitric oxide in MDM cell supernatants from stimulated cells in comparison to unstimulated controls are shown in c . Supernatants and mRNA were harvested contemporaneously from the same cell populations and were performed in triplicate. Data from the same representative individual are shown in a , b and c

    Journal: BMC Veterinary Research

    Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

    doi: 10.1186/s12917-019-1785-0

    Figure Lengend Snippet: Fold changes in MDM production of nos2 ( a ) and arg1 ( b ) following stimulation with LPS (1000 ng/ml) IFNγ (20 ng/ml) or IL-4 (20 ng/ml) IL-13 (20 ng/ml) compared with changes in control cells, normalised with reference to transcription levels of GAPDH at 12 24 h. Fold changes in levels of nitric oxide in MDM cell supernatants from stimulated cells in comparison to unstimulated controls are shown in c . Supernatants and mRNA were harvested contemporaneously from the same cell populations and were performed in triplicate. Data from the same representative individual are shown in a , b and c

    Article Snippet: Cell stimulation MDM or SM were stimulated by addition of 50–2000 ng/ml LPS from E. coli 0111:B4 (Insight Biotechnology Ltd., Middlesex) and/or 20-100 ng/ml bovine IFNγ (ThermoFisher Scientific, UK) or with 10-40 ng/ml recombinant bovine IL-4 (Kingfisher Biotech Inc., St Paul) and/or 10-20 ng/ml recombinant bovine IL-13 (Kingfisher Biotech Inc).

    Techniques:

    Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h

    Journal: BMC Veterinary Research

    Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

    doi: 10.1186/s12917-019-1785-0

    Figure Lengend Snippet: Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h

    Article Snippet: Cell stimulation MDM or SM were stimulated by addition of 50–2000 ng/ml LPS from E. coli 0111:B4 (Insight Biotechnology Ltd., Middlesex) and/or 20-100 ng/ml bovine IFNγ (ThermoFisher Scientific, UK) or with 10-40 ng/ml recombinant bovine IL-4 (Kingfisher Biotech Inc., St Paul) and/or 10-20 ng/ml recombinant bovine IL-13 (Kingfisher Biotech Inc).

    Techniques: Derivative Assay

    IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or IL-4 (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown

    Journal: BMC Veterinary Research

    Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

    doi: 10.1186/s12917-019-1785-0

    Figure Lengend Snippet: IL-6 concentration in supernatants from splenic macrophages stimulated with LPS (1000 ng/ml) and IFNγ (20 ng/ml) or IL-4 (20 ng/ml) for 20 h. OD values were determined from samples comprising combined supernatants from triplicate wells, run in duplicate. Results for two individual animals ( a and b ) are shown

    Article Snippet: MDM or SM were stimulated by addition of 50–2000 ng/ml LPS from E. coli 0111:B4 (Insight Biotechnology Ltd., Middlesex) and/or 20-100 ng/ml bovine IFNγ (ThermoFisher Scientific, UK) or with 10-40 ng/ml recombinant bovine IL-4 (Kingfisher Biotech Inc., St Paul) and/or 10-20 ng/ml recombinant bovine IL-13 (Kingfisher Biotech Inc).

    Techniques: Concentration Assay

    Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h

    Journal: BMC Veterinary Research

    Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

    doi: 10.1186/s12917-019-1785-0

    Figure Lengend Snippet: Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h

    Article Snippet: MDM or SM were stimulated by addition of 50–2000 ng/ml LPS from E. coli 0111:B4 (Insight Biotechnology Ltd., Middlesex) and/or 20-100 ng/ml bovine IFNγ (ThermoFisher Scientific, UK) or with 10-40 ng/ml recombinant bovine IL-4 (Kingfisher Biotech Inc., St Paul) and/or 10-20 ng/ml recombinant bovine IL-13 (Kingfisher Biotech Inc).

    Techniques: Derivative Assay

    The percent positive cells or MdFI of CD14 (A) , CD86 (B) , and CD205 (C , D) expressed by bovine MoDC generated from enriched monocytes via MACS and plate adherence (MACS+Adh) and cultured in AIM-V serum-free medium (AV-SF) for 4 days. MoDC were differentiated using various concentrations of recombinant bovine IL-4 (rbIL-4) and recombinant bovine granulocyte-macrophage colony-stimulating factor (rbGM-CSF). Each circle represents that data point for each individual animal (n = 3) used for this experiment. Horizontal lines represent the mean ± SEM for that condition. *P

    Journal: Frontiers in Immunology

    Article Title: Generating Bovine Monocyte-Derived Dendritic Cells for Experimental and Clinical Applications Using Commercially Available Serum-Free Medium

    doi: 10.3389/fimmu.2020.591185

    Figure Lengend Snippet: The percent positive cells or MdFI of CD14 (A) , CD86 (B) , and CD205 (C , D) expressed by bovine MoDC generated from enriched monocytes via MACS and plate adherence (MACS+Adh) and cultured in AIM-V serum-free medium (AV-SF) for 4 days. MoDC were differentiated using various concentrations of recombinant bovine IL-4 (rbIL-4) and recombinant bovine granulocyte-macrophage colony-stimulating factor (rbGM-CSF). Each circle represents that data point for each individual animal (n = 3) used for this experiment. Horizontal lines represent the mean ± SEM for that condition. *P

    Article Snippet: The adhered, enriched monocytes were then supplemented with fresh medium containing 250 ng/ml recombinant bovine interleukin 4 (rbIL-4) and 25 ng/ml recombinant bovine granulocyte-macrophage colony-stimulating factor (rbGM-CSF) sourced from Kingfisher Biotech (Saint Paul, MN) and cells were incubated at 37°C and 5% CO2 .

    Techniques: Generated, Magnetic Cell Separation, Cell Culture, Recombinant

    Dendritic cell maturation assays . Immature monocyte-derived dendritic cells (iMDDCs) were generated by adding IL-4 and GM-CSF to fresh monocyte cultures. After 6 days of differentiation, 1 × 10 6 sorted γδ T lymphocytes were added to wells containing 2 × 10 5 iMDDCs for 48 h (iMDDC + WC1 + and iMDDC + WC1 neg ). On day 6, live Mycobacterium avium subspecies paratuberculosis was added at a multiplicity of infection of 10:1 to evaluate if it affected iMDDC maturation after 48 h.

    Journal: Frontiers in Immunology

    Article Title: Bovine WC1+ and WC1neg γδ T Lymphocytes Influence Monocyte Differentiation and Monocyte-Derived Dendritic Cell Maturation during In Vitro Mycobacterium avium Subspecies paratuberculosis Infection

    doi: 10.3389/fimmu.2017.00534

    Figure Lengend Snippet: Dendritic cell maturation assays . Immature monocyte-derived dendritic cells (iMDDCs) were generated by adding IL-4 and GM-CSF to fresh monocyte cultures. After 6 days of differentiation, 1 × 10 6 sorted γδ T lymphocytes were added to wells containing 2 × 10 5 iMDDCs for 48 h (iMDDC + WC1 + and iMDDC + WC1 neg ). On day 6, live Mycobacterium avium subspecies paratuberculosis was added at a multiplicity of infection of 10:1 to evaluate if it affected iMDDC maturation after 48 h.

    Article Snippet: To obtain iMDDCs, complete RPMI was supplemented with 200 ng/mL of recombinant bovine interleukin-4 (Kingfisher Biotech, MN, USA) and 100 ng/mL of recombinant bovine GM-CSF (Kingfisher Biotech).

    Techniques: Derivative Assay, Generated, Infection