rna clean concentratortm 5 kit  (Zymo Research)


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    Name:
    RNA Clean Concentrator 5
    Description:
    The RNA Clean Concentrator kits provide a simple and reliable method for the rapid preparation of high quality RT PCR ready DNA free R1013 R1014 RNA This simple procedure is based on the use of a unique single buffer system and Zymo Spin column technology that allows for selective recovery of total RNA 17 nt large RNAs 200 nt and or small RNAs 17 200 nt The procedure is easy Add binding buffer and ethanol to your sample then bind wash and elute ultra pure RNA The RNA can be eluted from the Zymo Spin IC Column in as little as 6 µl of RNase free water The highly concentrated purified RNA is suitable for all subsequent analyses and molecular manipulations
    Catalog Number:
    r1013
    Price:
    None
    Applications:
    RNA Purification
    Size:
    50 units
    Category:
    Life Science Reagents and Media
    Buy from Supplier


    Structured Review

    Zymo Research rna clean concentratortm 5 kit
    RNA Clean Concentrator 5
    The RNA Clean Concentrator kits provide a simple and reliable method for the rapid preparation of high quality RT PCR ready DNA free R1013 R1014 RNA This simple procedure is based on the use of a unique single buffer system and Zymo Spin column technology that allows for selective recovery of total RNA 17 nt large RNAs 200 nt and or small RNAs 17 200 nt The procedure is easy Add binding buffer and ethanol to your sample then bind wash and elute ultra pure RNA The RNA can be eluted from the Zymo Spin IC Column in as little as 6 µl of RNase free water The highly concentrated purified RNA is suitable for all subsequent analyses and molecular manipulations
    https://www.bioz.com/result/rna clean concentratortm 5 kit/product/Zymo Research
    Average 95 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    rna clean concentratortm 5 kit - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Centrifugation:

    Article Title: The YTH Domain Protein ECT2 Is an m6A Reader Required for Normal Trichome Branching in Arabidopsis [OPEN]
    Article Snippet: RNAs were precipitated by centrifugation, resuspended in 350 μL of RTL buffer (Qiagen RNEasy Micro kit), purified according to the manufacturer’s instructions, and eluted in 15 μL of RNase-free water. .. The RNA solution was concentrated into a 6 μL volume of RNase-free water using the RNA clean and concentrator kit R1015 from Zymo Research.

    Amplification:

    Article Title: A Genome-Wide Survey of Highly Expressed Non-Coding RNAs and Biological Validation of Selected Candidates in Agrobacterium tumefaciens
    Article Snippet: The detailed protocol describes the steps for total RNA fragmentation, adapter ligation, reverse transcription, PCR amplification, purification, cluster generation and sequencing, and can be found in Illumina TruSeq Small RNA SamplePrep Guide (#15004197). .. The fragmented total-RNA samples (small RNA) were purified with procedure 1 of the RNA clean & concentrator-5 (part#R1015, Zymo Research), and were then eluted with 6 µl of RNase-free water.

    Article Title: Robust B Cell Responses Predict Rapid Resolution of Lyme Disease
    Article Snippet: Total RNA from Lyme and control PBMCs was extracted with Trizol (Thermo Fisher) and subsequently purified using RNA Clean & Concentrator-5 (Zymo Research) according to the manufacturer’s procedure. .. UMI-barcoded cDNA was amplified with SeqAmp DNA polymerase (Takara Bio USA, Inc.) using the 5′ bridge sequence and gene-specific reverse sequences.

    Article Title: SRSF9 selectively represses ADAR2-mediated editing of brain-specific sites in primates
    Article Snippet: The RNA was then extracted by phenol:chloroform method and concentrated using RNA Clean & Concentrator-5 (Zymo research). .. The eluted RNA was reverse transcribed, ligated with adaptors and PCR amplified to make the library.

    Article Title: Comparison between NuGEN's WT-Ovation Pico and One-Direct Amplification Systems
    Article Snippet: RNA samples for amplification with the Pico kit were isolated from 150 control cells or from 80 giant cells using the PicoPure RNA isolation kit (Arcturus, Mountain View, CA, USA) with the optional DNase treatment, according to the manufacturer's instructions. .. Total RNA was concentrated and cleaned using a RNA Clean & Concentrator-5 (Zymo Research, Orange, CA, USA).

    Article Title: Insertion of a chimeric retrotransposon sequence in mouse Axin1 locus causes metastable kinky tail phenotype
    Article Snippet: The gRNAs were in vitro transcribed using HiScribe™ T7 High Yield RNA Synthesis Kit (E2040S, NEB) and purified using RNA Clean & Concentrator™-5 (R1013, Zymo Research). .. The IAP LTR and the chimeric L1 were synthesized by IDT, and two homology arms (~ 1 kb) flanking the gRNA cutting sites of A or Axin1 locus were amplified by Q5® Hot Start High-Fidelity 2X Master Mix (M0494S, NEB) from mouse tail genomic DNA.

    Synthesized:

    Article Title: Insertion of a chimeric retrotransposon sequence in mouse Axin1 locus causes metastable kinky tail phenotype
    Article Snippet: The gRNAs were in vitro transcribed using HiScribe™ T7 High Yield RNA Synthesis Kit (E2040S, NEB) and purified using RNA Clean & Concentrator™-5 (R1013, Zymo Research). .. The IAP LTR and the chimeric L1 were synthesized by IDT, and two homology arms (~ 1 kb) flanking the gRNA cutting sites of A or Axin1 locus were amplified by Q5® Hot Start High-Fidelity 2X Master Mix (M0494S, NEB) from mouse tail genomic DNA.

    Quantitative RT-PCR:

    Article Title: 2,4-Thiazolidinedione Treatment Improves the Innate Immune Response in Dairy Goats with Induced Subclinical Mastitis
    Article Snippet: .. RNA Isolation and Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) RNA extraction was performed using RNA Clean & Concentrator™-5 (cat# R1013, Zymo Research, USA) following the vendor protocol. .. Prior to RNA extraction, the adipose tissue was disrupted using a Bullet Blender Next Advance (Laboratory Instruments, USA).

    Concentration Assay:

    Article Title: Transcriptome-wide mapping of signaling pathways and early immune responses in lumpfish leukocytes upon in vitro bacterial exposure
    Article Snippet: Samples were treated with DNase I (Sigma) to removed traces of genomic DNA and the concentration of total RNA determined in a Nanodrop® ND-1000 UV-Vis spectrophotometer (Nanodrop Technologies). .. The pooled RNA (5 µg) was cleaned using RNA clean & concentrator-5 (zymo research) according to the manufacturer’s instructions and the quality of the RNA were determined in an Agilent 2100 bioanalyzer.

    Real-time Polymerase Chain Reaction:

    Article Title: The function and molecular identity of inward rectifier channels in vestibular hair cells of the mouse inner ear
    Article Snippet: Paragraph title: qPCR. ... RNA was purified using an RNA purification kit (DNA-Free RNA Kit; Cat. #R1013, Zymo Research, Irvine, CA).

    Article Title: 2,4-Thiazolidinedione Treatment Improves the Innate Immune Response in Dairy Goats with Induced Subclinical Mastitis
    Article Snippet: .. RNA Isolation and Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) RNA extraction was performed using RNA Clean & Concentrator™-5 (cat# R1013, Zymo Research, USA) following the vendor protocol. .. Prior to RNA extraction, the adipose tissue was disrupted using a Bullet Blender Next Advance (Laboratory Instruments, USA).

    Incubation:

    Article Title: A Genome-Wide Survey of Highly Expressed Non-Coding RNAs and Biological Validation of Selected Candidates in Agrobacterium tumefaciens
    Article Snippet: A 25 µl reaction mixture was incubated at 37°C for 1 hour on a pre-heated thermal cycler. .. The fragmented total-RNA samples (small RNA) were purified with procedure 1 of the RNA clean & concentrator-5 (part#R1015, Zymo Research), and were then eluted with 6 µl of RNase-free water.

    Article Title: SRSF9 selectively represses ADAR2-mediated editing of brain-specific sites in primates
    Article Snippet: The cleared lysates were incubated with MYC-coupled Protein G Dynabeads (Invitrogen) for 4 h at 4°C, and then eluted and transferred overnight onto nitrocellulose membrane. .. The RNA was then extracted by phenol:chloroform method and concentrated using RNA Clean & Concentrator-5 (Zymo research).

    Article Title: A Rapid Spin Column-Based Method to Enrich Pathogen Transcripts from Eukaryotic Host Cells Prior to Sequencing
    Article Snippet: .. The RNA was next fragmented using the NEBNext Magnesium RNA Fragmentation Module [ ] with a 3 minute incubation at 94°C followed by a cleanup step using the RNA Clean & Concentrator-5 (Zymo Research). .. Double-stranded, tagged cDNA was generated from 50 ng of fragmented RNA by the Peregrine method as previously described [ , ].

    Article Title: The YTH Domain Protein ECT2 Is an m6A Reader Required for Normal Trichome Branching in Arabidopsis [OPEN]
    Article Snippet: To monitor protein immunoprecipitation efficiency, a parallel immunoprecipitation experiment was conducted using 700 μL of crude extract incubated with 7.5 μL of GFP-trap. .. The RNA solution was concentrated into a 6 μL volume of RNase-free water using the RNA clean and concentrator kit R1015 from Zymo Research.

    Knock-In:

    Article Title: Insertion of a chimeric retrotransposon sequence in mouse Axin1 locus causes metastable kinky tail phenotype
    Article Snippet: Paragraph title: Generation of knock-in mice and breeding scheme ... The gRNAs were in vitro transcribed using HiScribe™ T7 High Yield RNA Synthesis Kit (E2040S, NEB) and purified using RNA Clean & Concentrator™-5 (R1013, Zymo Research).

    Over Expression:

    Article Title: SRSF9 selectively represses ADAR2-mediated editing of brain-specific sites in primates
    Article Snippet: Briefly, HEK293T cells overexpressing SRSF9 (with or without ADAR2 overexpression) were ultraviolet-irradiated at 254 nm (UV-C), 1500 mJ cm2 (Stratagene Stratalinker). .. The RNA was then extracted by phenol:chloroform method and concentrated using RNA Clean & Concentrator-5 (Zymo research).

    Ligation:

    Article Title: A Genome-Wide Survey of Highly Expressed Non-Coding RNAs and Biological Validation of Selected Candidates in Agrobacterium tumefaciens
    Article Snippet: The detailed protocol describes the steps for total RNA fragmentation, adapter ligation, reverse transcription, PCR amplification, purification, cluster generation and sequencing, and can be found in Illumina TruSeq Small RNA SamplePrep Guide (#15004197). .. The fragmented total-RNA samples (small RNA) were purified with procedure 1 of the RNA clean & concentrator-5 (part#R1015, Zymo Research), and were then eluted with 6 µl of RNase-free water.

    Infection:

    Article Title: Transcriptome-wide mapping of signaling pathways and early immune responses in lumpfish leukocytes upon in vitro bacterial exposure
    Article Snippet: The pooled RNA (5 µg) was cleaned using RNA clean & concentrator-5 (zymo research) according to the manufacturer’s instructions and the quality of the RNA were determined in an Agilent 2100 bioanalyzer. .. RNA isolated from virus infected leukocytes was kept separately.

    Generated:

    Article Title: A Rapid Spin Column-Based Method to Enrich Pathogen Transcripts from Eukaryotic Host Cells Prior to Sequencing
    Article Snippet: The RNA was next fragmented using the NEBNext Magnesium RNA Fragmentation Module [ ] with a 3 minute incubation at 94°C followed by a cleanup step using the RNA Clean & Concentrator-5 (Zymo Research). .. Double-stranded, tagged cDNA was generated from 50 ng of fragmented RNA by the Peregrine method as previously described [ , ].

    Article Title: Analysis of chromatin accessibility uncovers TEAD1 as a regulator of migration in human glioblastoma
    Article Snippet: .. Total RNA was isolated from cell lines via Trizol/chloroform/isopropanol extraction using standard protocol (ThermoFisher, 15596–026), followed by RNA cleanup-and-concentrator kit with DNase treatment (Zymo Research, R1013). cDNA was generated using the High-Capacity RNA-to-cDNA Kit (Life Technologies, 4387406). .. RT-qPCR reactions were run in duplicates, and Ct values were normalized to the ACTB housekeeping gene.

    Article Title: Insertion of a chimeric retrotransposon sequence in mouse Axin1 locus causes metastable kinky tail phenotype
    Article Snippet: The gRNAs were in vitro transcribed using HiScribe™ T7 High Yield RNA Synthesis Kit (E2040S, NEB) and purified using RNA Clean & Concentrator™-5 (R1013, Zymo Research). .. Donor DNA templates that contain homology arms and the IAP LTR or the chimeric L1 were generated with NEBuilder® HiFi DNA Assembly Master Mix (E2621L, NEB).

    Sequencing:

    Article Title: A Genome-Wide Survey of Highly Expressed Non-Coding RNAs and Biological Validation of Selected Candidates in Agrobacterium tumefaciens
    Article Snippet: The detailed protocol describes the steps for total RNA fragmentation, adapter ligation, reverse transcription, PCR amplification, purification, cluster generation and sequencing, and can be found in Illumina TruSeq Small RNA SamplePrep Guide (#15004197). .. The fragmented total-RNA samples (small RNA) were purified with procedure 1 of the RNA clean & concentrator-5 (part#R1015, Zymo Research), and were then eluted with 6 µl of RNase-free water.

    Article Title: Robust B Cell Responses Predict Rapid Resolution of Lyme Disease
    Article Snippet: Paragraph title: Bulk Heavy-Chain Sequencing ... Total RNA from Lyme and control PBMCs was extracted with Trizol (Thermo Fisher) and subsequently purified using RNA Clean & Concentrator-5 (Zymo Research) according to the manufacturer’s procedure.

    Sonication:

    Article Title: SRSF9 selectively represses ADAR2-mediated editing of brain-specific sites in primates
    Article Snippet: Subsequently, the cells were lysed in iCLIP lysis buffer with short pulses of sonication using the bioruptor. .. The RNA was then extracted by phenol:chloroform method and concentrated using RNA Clean & Concentrator-5 (Zymo research).

    RNA Sequencing Assay:

    Article Title: Transcriptome-wide mapping of signaling pathways and early immune responses in lumpfish leukocytes upon in vitro bacterial exposure
    Article Snippet: For each time point three parallels were prepared for RNA sequencing. .. The pooled RNA (5 µg) was cleaned using RNA clean & concentrator-5 (zymo research) according to the manufacturer’s instructions and the quality of the RNA were determined in an Agilent 2100 bioanalyzer.

    Article Title: Analysis of chromatin accessibility uncovers TEAD1 as a regulator of migration in human glioblastoma
    Article Snippet: All RNA-seq tests were FDR adjusted for multiple testing correction. .. Total RNA was isolated from cell lines via Trizol/chloroform/isopropanol extraction using standard protocol (ThermoFisher, 15596–026), followed by RNA cleanup-and-concentrator kit with DNase treatment (Zymo Research, R1013). cDNA was generated using the High-Capacity RNA-to-cDNA Kit (Life Technologies, 4387406).

    Magnetic Beads:

    Article Title: The YTH Domain Protein ECT2 Is an m6A Reader Required for Normal Trichome Branching in Arabidopsis [OPEN]
    Article Snippet: The immunoprecipitation was performed using 11.25 mL of crude extract incubated for 1.5 h at 4°C on a rotating wheel with 120 μL of GFP-trap magnetic beads (Chromotek). .. The RNA solution was concentrated into a 6 μL volume of RNase-free water using the RNA clean and concentrator kit R1015 from Zymo Research.

    Mutagenesis:

    Article Title: A Genome-Wide Survey of Highly Expressed Non-Coding RNAs and Biological Validation of Selected Candidates in Agrobacterium tumefaciens
    Article Snippet: The fragmented total-RNA samples (small RNA) were purified with procedure 1 of the RNA clean & concentrator-5 (part#R1015, Zymo Research), and were then eluted with 6 µl of RNase-free water. .. Next, a master mix of 4 µl was prepared as following before adding to the 6 µl reaction: 2 µl Ligation Buffer (HML) (part#15013206, Illumina), 1 µl RNase Inhibitor (part#15003548, Illumina), and 1 µl T4 RNA Ligase 2 Deletion Mutant (part# M0242S, NEB).

    Isolation:

    Article Title: The function and molecular identity of inward rectifier channels in vestibular hair cells of the mouse inner ear
    Article Snippet: RNA was isolated using an RNAqueous-Micro kit (Cat. #1931, Ambion, Austin, TX). .. RNA was purified using an RNA purification kit (DNA-Free RNA Kit; Cat. #R1013, Zymo Research, Irvine, CA).

    Article Title: Single-cell full-length total RNA sequencing uncovers dynamics of recursive splicing and enhancer RNAs
    Article Snippet: We prepared both poly(A)-depleted RNA and poly(A)-selected RNA from each 1 µg of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) as follows. .. Purified poly(A)-depleted RNA was also obtained from the supernatant using RNA Clean & Concentrator-5 (Zymo Research).

    Article Title: Transcriptome-wide mapping of signaling pathways and early immune responses in lumpfish leukocytes upon in vitro bacterial exposure
    Article Snippet: Paragraph title: Isolation of total RNA ... The pooled RNA (5 µg) was cleaned using RNA clean & concentrator-5 (zymo research) according to the manufacturer’s instructions and the quality of the RNA were determined in an Agilent 2100 bioanalyzer.

    Article Title: 2,4-Thiazolidinedione Treatment Improves the Innate Immune Response in Dairy Goats with Induced Subclinical Mastitis
    Article Snippet: .. RNA Isolation and Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) RNA extraction was performed using RNA Clean & Concentrator™-5 (cat# R1013, Zymo Research, USA) following the vendor protocol. .. Prior to RNA extraction, the adipose tissue was disrupted using a Bullet Blender Next Advance (Laboratory Instruments, USA).

    Article Title: Analysis of chromatin accessibility uncovers TEAD1 as a regulator of migration in human glioblastoma
    Article Snippet: .. Total RNA was isolated from cell lines via Trizol/chloroform/isopropanol extraction using standard protocol (ThermoFisher, 15596–026), followed by RNA cleanup-and-concentrator kit with DNase treatment (Zymo Research, R1013). cDNA was generated using the High-Capacity RNA-to-cDNA Kit (Life Technologies, 4387406). .. RT-qPCR reactions were run in duplicates, and Ct values were normalized to the ACTB housekeeping gene.

    Article Title: Comparison between NuGEN's WT-Ovation Pico and One-Direct Amplification Systems
    Article Snippet: RNA samples for amplification with the Pico kit were isolated from 150 control cells or from 80 giant cells using the PicoPure RNA isolation kit (Arcturus, Mountain View, CA, USA) with the optional DNase treatment, according to the manufacturer's instructions. .. Total RNA was concentrated and cleaned using a RNA Clean & Concentrator-5 (Zymo Research, Orange, CA, USA).

    RNA Extraction:

    Article Title: 2,4-Thiazolidinedione Treatment Improves the Innate Immune Response in Dairy Goats with Induced Subclinical Mastitis
    Article Snippet: .. RNA Isolation and Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) RNA extraction was performed using RNA Clean & Concentrator™-5 (cat# R1013, Zymo Research, USA) following the vendor protocol. .. Prior to RNA extraction, the adipose tissue was disrupted using a Bullet Blender Next Advance (Laboratory Instruments, USA).

    Article Title: A Rapid Spin Column-Based Method to Enrich Pathogen Transcripts from Eukaryotic Host Cells Prior to Sequencing
    Article Snippet: Paragraph title: RNA extraction and cDNA Synthesis ... The RNA was next fragmented using the NEBNext Magnesium RNA Fragmentation Module [ ] with a 3 minute incubation at 94°C followed by a cleanup step using the RNA Clean & Concentrator-5 (Zymo Research).

    Purification:

    Article Title: A Genome-Wide Survey of Highly Expressed Non-Coding RNAs and Biological Validation of Selected Candidates in Agrobacterium tumefaciens
    Article Snippet: .. The fragmented total-RNA samples (small RNA) were purified with procedure 1 of the RNA clean & concentrator-5 (part#R1015, Zymo Research), and were then eluted with 6 µl of RNase-free water. .. Five microliter of purified fragmented RNAs were ligated with 1 µl RNA 3′ Adapter (RA3) (part# 15013207, Illumina); reactions were heated at 70°C for 2 minutes, then immediately cooled on ice.

    Article Title: The function and molecular identity of inward rectifier channels in vestibular hair cells of the mouse inner ear
    Article Snippet: .. RNA was purified using an RNA purification kit (DNA-Free RNA Kit; Cat. #R1013, Zymo Research, Irvine, CA). .. To eliminate genomic DNA and reverse transcribe isolated RNA into cDNA, we used the QuantiTect reverse transcription kit (Qiagen, Valencia, CA).

    Article Title: Single-cell full-length total RNA sequencing uncovers dynamics of recursive splicing and enhancer RNAs
    Article Snippet: .. Purified poly(A)-depleted RNA was also obtained from the supernatant using RNA Clean & Concentrator-5 (Zymo Research). .. We performed RT-qPCR using the abovementioned total RNA, poly(A)-depleted RNA, and poly(A)-selected RNA.

    Article Title: Robust B Cell Responses Predict Rapid Resolution of Lyme Disease
    Article Snippet: .. Total RNA from Lyme and control PBMCs was extracted with Trizol (Thermo Fisher) and subsequently purified using RNA Clean & Concentrator-5 (Zymo Research) according to the manufacturer’s procedure. .. Purified RNA was tagged with UMI sequences and a common primer site by template switching during reverse transcription (SmartScribe Reverse Transcriptase, Takara Bio USA, Inc., Mountain View, CA, USA).

    Article Title: Shifts in the Gut Metabolome and Clostridium difficile Transcriptome throughout Colonization and Infection in a Mouse Model
    Article Snippet: .. RNA samples were depleted of DNA by two rounds of treatment with Turbo DNase (Thermo Fisher) per the manufacturer’s protocol; all samples were column purified with a Zymo Clean and Concentrator kit (R1015). .. Depletion of contaminating genomic DNA was confirmed via PCR performed with rpoC primers (see in the supplemental material).

    Article Title: The YTH Domain Protein ECT2 Is an m6A Reader Required for Normal Trichome Branching in Arabidopsis [OPEN]
    Article Snippet: RNAs were precipitated by centrifugation, resuspended in 350 μL of RTL buffer (Qiagen RNEasy Micro kit), purified according to the manufacturer’s instructions, and eluted in 15 μL of RNase-free water. .. The RNA solution was concentrated into a 6 μL volume of RNase-free water using the RNA clean and concentrator kit R1015 from Zymo Research.

    Article Title: Insertion of a chimeric retrotransposon sequence in mouse Axin1 locus causes metastable kinky tail phenotype
    Article Snippet: .. The gRNAs were in vitro transcribed using HiScribe™ T7 High Yield RNA Synthesis Kit (E2040S, NEB) and purified using RNA Clean & Concentrator™-5 (R1013, Zymo Research). .. Cas9 mRNA was purchased from TriLink BioTechnologies (L7606).

    Dot Blot:

    Article Title: The YTH Domain Protein ECT2 Is an m6A Reader Required for Normal Trichome Branching in Arabidopsis [OPEN]
    Article Snippet: Paragraph title: RIP and m6 A Dot Blot Assays ... The RNA solution was concentrated into a 6 μL volume of RNase-free water using the RNA clean and concentrator kit R1015 from Zymo Research.

    Polymerase Chain Reaction:

    Article Title: A Genome-Wide Survey of Highly Expressed Non-Coding RNAs and Biological Validation of Selected Candidates in Agrobacterium tumefaciens
    Article Snippet: The detailed protocol describes the steps for total RNA fragmentation, adapter ligation, reverse transcription, PCR amplification, purification, cluster generation and sequencing, and can be found in Illumina TruSeq Small RNA SamplePrep Guide (#15004197). .. The fragmented total-RNA samples (small RNA) were purified with procedure 1 of the RNA clean & concentrator-5 (part#R1015, Zymo Research), and were then eluted with 6 µl of RNase-free water.

    Article Title: Robust B Cell Responses Predict Rapid Resolution of Lyme Disease
    Article Snippet: Total RNA from Lyme and control PBMCs was extracted with Trizol (Thermo Fisher) and subsequently purified using RNA Clean & Concentrator-5 (Zymo Research) according to the manufacturer’s procedure. .. This product was then split into two separate reactions per sample, for which Illumina sequencing adapters were added by overlap extension PCR in opposite orientations between the two reactions using Q5 DNA Polymerase (New England Biolabs, Ipswich, MA, USA).

    Article Title: SRSF9 selectively represses ADAR2-mediated editing of brain-specific sites in primates
    Article Snippet: The RNA was then extracted by phenol:chloroform method and concentrated using RNA Clean & Concentrator-5 (Zymo research). .. The eluted RNA was reverse transcribed, ligated with adaptors and PCR amplified to make the library.

    Article Title: Shifts in the Gut Metabolome and Clostridium difficile Transcriptome throughout Colonization and Infection in a Mouse Model
    Article Snippet: RNA samples were depleted of DNA by two rounds of treatment with Turbo DNase (Thermo Fisher) per the manufacturer’s protocol; all samples were column purified with a Zymo Clean and Concentrator kit (R1015). .. Depletion of contaminating genomic DNA was confirmed via PCR performed with rpoC primers (see in the supplemental material).

    cDNA Library Assay:

    Article Title: A Genome-Wide Survey of Highly Expressed Non-Coding RNAs and Biological Validation of Selected Candidates in Agrobacterium tumefaciens
    Article Snippet: Paragraph title: cDNA library preparation ... The fragmented total-RNA samples (small RNA) were purified with procedure 1 of the RNA clean & concentrator-5 (part#R1015, Zymo Research), and were then eluted with 6 µl of RNase-free water.

    Mouse Assay:

    Article Title: The function and molecular identity of inward rectifier channels in vestibular hair cells of the mouse inner ear
    Article Snippet: Utricles were acutely dissected from pre- and postnatal wild-type mice at six different ages: E15 ( n = 20), E18 ( n = 15), P0 ( n = 24), P7 ( n = 14), P25 ( n = 30), and P180 ( n = 14). .. RNA was purified using an RNA purification kit (DNA-Free RNA Kit; Cat. #R1013, Zymo Research, Irvine, CA).

    Article Title: Insertion of a chimeric retrotransposon sequence in mouse Axin1 locus causes metastable kinky tail phenotype
    Article Snippet: Paragraph title: Generation of knock-in mice and breeding scheme ... The gRNAs were in vitro transcribed using HiScribe™ T7 High Yield RNA Synthesis Kit (E2040S, NEB) and purified using RNA Clean & Concentrator™-5 (R1013, Zymo Research).

    Functional Assay:

    Article Title: Analysis of chromatin accessibility uncovers TEAD1 as a regulator of migration in human glioblastoma
    Article Snippet: Functional enrichment analyses were performed using GSEA, DAVID, and Pantherdb tools (see Supplementary references). .. Total RNA was isolated from cell lines via Trizol/chloroform/isopropanol extraction using standard protocol (ThermoFisher, 15596–026), followed by RNA cleanup-and-concentrator kit with DNase treatment (Zymo Research, R1013). cDNA was generated using the High-Capacity RNA-to-cDNA Kit (Life Technologies, 4387406).

    In Vitro:

    Article Title: Insertion of a chimeric retrotransposon sequence in mouse Axin1 locus causes metastable kinky tail phenotype
    Article Snippet: .. The gRNAs were in vitro transcribed using HiScribe™ T7 High Yield RNA Synthesis Kit (E2040S, NEB) and purified using RNA Clean & Concentrator™-5 (R1013, Zymo Research). .. Cas9 mRNA was purchased from TriLink BioTechnologies (L7606).

    Fluorescence In Situ Hybridization:

    Article Title: Transcriptome-wide mapping of signaling pathways and early immune responses in lumpfish leukocytes upon in vitro bacterial exposure
    Article Snippet: Total RNA extracts from three-five fish were pooled, in total 5 µg per pooled sample. .. The pooled RNA (5 µg) was cleaned using RNA clean & concentrator-5 (zymo research) according to the manufacturer’s instructions and the quality of the RNA were determined in an Agilent 2100 bioanalyzer.

    Spectrophotometry:

    Article Title: Transcriptome-wide mapping of signaling pathways and early immune responses in lumpfish leukocytes upon in vitro bacterial exposure
    Article Snippet: Samples were treated with DNase I (Sigma) to removed traces of genomic DNA and the concentration of total RNA determined in a Nanodrop® ND-1000 UV-Vis spectrophotometer (Nanodrop Technologies). .. The pooled RNA (5 µg) was cleaned using RNA clean & concentrator-5 (zymo research) according to the manufacturer’s instructions and the quality of the RNA were determined in an Agilent 2100 bioanalyzer.

    Article Title: 2,4-Thiazolidinedione Treatment Improves the Innate Immune Response in Dairy Goats with Induced Subclinical Mastitis
    Article Snippet: RNA Isolation and Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) RNA extraction was performed using RNA Clean & Concentrator™-5 (cat# R1013, Zymo Research, USA) following the vendor protocol. .. RNA was quantified with a Nanodrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE).

    Article Title: Comparison between NuGEN's WT-Ovation Pico and One-Direct Amplification Systems
    Article Snippet: Total RNA was concentrated and cleaned using a RNA Clean & Concentrator-5 (Zymo Research, Orange, CA, USA). .. Total RNA was quantified using a Nanodrop Spectrophotometer 2000 (Thermo Scientific Inc., Waltham, MA, USA) and the quality confirmed on an Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).

    Immunoprecipitation:

    Article Title: The YTH Domain Protein ECT2 Is an m6A Reader Required for Normal Trichome Branching in Arabidopsis [OPEN]
    Article Snippet: To monitor protein immunoprecipitation efficiency, a parallel immunoprecipitation experiment was conducted using 700 μL of crude extract incubated with 7.5 μL of GFP-trap. .. The RNA solution was concentrated into a 6 μL volume of RNase-free water using the RNA clean and concentrator kit R1015 from Zymo Research.

    Lysis:

    Article Title: SRSF9 selectively represses ADAR2-mediated editing of brain-specific sites in primates
    Article Snippet: Subsequently, the cells were lysed in iCLIP lysis buffer with short pulses of sonication using the bioruptor. .. The RNA was then extracted by phenol:chloroform method and concentrated using RNA Clean & Concentrator-5 (Zymo research).

    Article Title: Comparison between NuGEN's WT-Ovation Pico and One-Direct Amplification Systems
    Article Snippet: Total RNA was concentrated and cleaned using a RNA Clean & Concentrator-5 (Zymo Research, Orange, CA, USA). .. For amplification with the One-Direct kit, samples containing 150 control or 80 giant cells were collected directly into 2 μl of the NuGEN lysis buffer.

    Article Title: The YTH Domain Protein ECT2 Is an m6A Reader Required for Normal Trichome Branching in Arabidopsis [OPEN]
    Article Snippet: The beads were washed six times with 9 mL of lysis buffer at 4°C. .. The RNA solution was concentrated into a 6 μL volume of RNase-free water using the RNA clean and concentrator kit R1015 from Zymo Research.

    Cross-linking Immunoprecipitation:

    Article Title: SRSF9 selectively represses ADAR2-mediated editing of brain-specific sites in primates
    Article Snippet: Paragraph title: Enhanced CLIP ... The RNA was then extracted by phenol:chloroform method and concentrated using RNA Clean & Concentrator-5 (Zymo research).

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  • 95
    Zymo Research rna clean concentratortm 5 kit
    Rna Clean Concentratortm 5 Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna clean concentratortm 5 kit/product/Zymo Research
    Average 95 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    rna clean concentratortm 5 kit - by Bioz Stars, 2020-02
    95/100 stars
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    95
    Zymo Research rna clean concentratortm 25 kit
    Rna Clean Concentratortm 25 Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 1 article reviews
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    rna clean concentratortm 25 kit - by Bioz Stars, 2020-02
    95/100 stars
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