Journal: bioRxiv
Article Title: Latitudinal cline does not predict variation in the microbiome of wild Drosophila melanogaster
doi: 10.1101/2022.02.19.481158
Figure Lengend Snippet: PCoA plot for the effects of BstZ17I restriction digest on substrate samples. BstZ17I digest reduces the amount of Wolbachia amplicons, but not other common bacteria in the fly microbiome. To ensure that the BstZ17I digestion did not affect the apple or grape samples, we sequenced in parallel libraries without (circles, p93.12_nodig) and with the digest (star, p93.13_dig). Points are colored by the name of the orchards or vineyard and represent PCoA based on Bray-Curtis dissimilarity. There was no significant effects of the digest on apple or grape microbiomes (PERMANOVA: F 1,16 = 0.31, r 2 = 0.02, p=0.91).
Article Snippet: Samples (200-400 ng DNA) were digested with BstZ17I enzyme (NEB R3594) following manufacturer’s instructions (37ºC for 20 min).
Techniques: