bstz17i  (New England Biolabs)


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  • 92
    Name:
    BstZ17I HF
    Description:
    BstZ17I HF 5 000 units
    Catalog Number:
    r3594l
    Price:
    282
    Size:
    5 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs bstz17i
    BstZ17I HF
    BstZ17I HF 5 000 units
    https://www.bioz.com/result/bstz17i/product/New England Biolabs
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    bstz17i - by Bioz Stars, 2020-09
    92/100 stars

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    Related Articles

    Clone Assay:

    Article Title: The Eukaryotic Proteome is Shaped by E3 Ubiquitin Ligases Targeting C-terminal Degrons
    Article Snippet: .. The pool of barcoded vectors was then linearized with BstZ17I (NEB), gel purified (Qiagen QIAEX II Gel Extraction Kit), and subjected to a Gateway LR recombination reaction (Thermo Fisher Scientific) using entry clones from the Ultimate ORF collection assembled into six pools containing approximately 3000 ORFs each. .. ORF-barcode pairs were subsequently mapped by linearizing the plasmids with I-SceI, followed by fragmentation (NEBNext dsDNA Fragmentase), end repair, dA tailing and adaptor ligation (NEBNext DNA Library Prep kit).

    Amplification:

    Article Title: Drug-Associated Changes in Amino Acid Residues in Gag p2, p7NC, and p6Gag/p6Pol in Human Immunodeficiency Virus Type 1 (HIV-1) Display a Dominant Effect on Replicative Fitness and Drug Response
    Article Snippet: .. Briefly, the molecular clone pLAI.2 ( ) was modified by site-directed mutagenesis to remove the SpeI restriction site at nucleotide position 7 to produce the molecular clone pLAI.4. pLAI.4 was then digested with the restriction enzymes SpeI (HIVLAI position 1553, New England Biolabs, Inc, Beverly, MA, NEB) and Bstz17I (HIVLAI position 3011, NEB) to remove the LAI gag-pol region, and a 1.5 kb fragment amplified from patient samples was inserted into the pLAI.4 backbone. .. Recombinant viruses were made with gag-pol alleles obtained from an HIV-infected pediatric subject (D1) enrolled in a protocol to examine the impact of HIV-1 genotype on therapy outcome.

    Mutagenesis:

    Article Title: Drug-Associated Changes in Amino Acid Residues in Gag p2, p7NC, and p6Gag/p6Pol in Human Immunodeficiency Virus Type 1 (HIV-1) Display a Dominant Effect on Replicative Fitness and Drug Response
    Article Snippet: .. Briefly, the molecular clone pLAI.2 ( ) was modified by site-directed mutagenesis to remove the SpeI restriction site at nucleotide position 7 to produce the molecular clone pLAI.4. pLAI.4 was then digested with the restriction enzymes SpeI (HIVLAI position 1553, New England Biolabs, Inc, Beverly, MA, NEB) and Bstz17I (HIVLAI position 3011, NEB) to remove the LAI gag-pol region, and a 1.5 kb fragment amplified from patient samples was inserted into the pLAI.4 backbone. .. Recombinant viruses were made with gag-pol alleles obtained from an HIV-infected pediatric subject (D1) enrolled in a protocol to examine the impact of HIV-1 genotype on therapy outcome.

    Purification:

    Article Title: The Eukaryotic Proteome is Shaped by E3 Ubiquitin Ligases Targeting C-terminal Degrons
    Article Snippet: .. The pool of barcoded vectors was then linearized with BstZ17I (NEB), gel purified (Qiagen QIAEX II Gel Extraction Kit), and subjected to a Gateway LR recombination reaction (Thermo Fisher Scientific) using entry clones from the Ultimate ORF collection assembled into six pools containing approximately 3000 ORFs each. .. ORF-barcode pairs were subsequently mapped by linearizing the plasmids with I-SceI, followed by fragmentation (NEBNext dsDNA Fragmentase), end repair, dA tailing and adaptor ligation (NEBNext DNA Library Prep kit).

    Modification:

    Article Title: Drug-Associated Changes in Amino Acid Residues in Gag p2, p7NC, and p6Gag/p6Pol in Human Immunodeficiency Virus Type 1 (HIV-1) Display a Dominant Effect on Replicative Fitness and Drug Response
    Article Snippet: .. Briefly, the molecular clone pLAI.2 ( ) was modified by site-directed mutagenesis to remove the SpeI restriction site at nucleotide position 7 to produce the molecular clone pLAI.4. pLAI.4 was then digested with the restriction enzymes SpeI (HIVLAI position 1553, New England Biolabs, Inc, Beverly, MA, NEB) and Bstz17I (HIVLAI position 3011, NEB) to remove the LAI gag-pol region, and a 1.5 kb fragment amplified from patient samples was inserted into the pLAI.4 backbone. .. Recombinant viruses were made with gag-pol alleles obtained from an HIV-infected pediatric subject (D1) enrolled in a protocol to examine the impact of HIV-1 genotype on therapy outcome.

    Gel Extraction:

    Article Title: The Eukaryotic Proteome is Shaped by E3 Ubiquitin Ligases Targeting C-terminal Degrons
    Article Snippet: .. The pool of barcoded vectors was then linearized with BstZ17I (NEB), gel purified (Qiagen QIAEX II Gel Extraction Kit), and subjected to a Gateway LR recombination reaction (Thermo Fisher Scientific) using entry clones from the Ultimate ORF collection assembled into six pools containing approximately 3000 ORFs each. .. ORF-barcode pairs were subsequently mapped by linearizing the plasmids with I-SceI, followed by fragmentation (NEBNext dsDNA Fragmentase), end repair, dA tailing and adaptor ligation (NEBNext DNA Library Prep kit).

    Plasmid Preparation:

    Article Title: Iron deficiency drives an autosomal dominant hypophosphatemic rickets (ADHR) phenotype in fibroblast growth factor-23 (Fgf23) knock-in mice
    Article Snippet: .. The neomycin resistance gene ( Neor ) flanked by LoxP sites was removed from the pBSK-PGKneoLox2 vector (the generous gift of Fred Dick, University of Ottawa, Ottawa) using an EcoRV-SmaI digest (New England Biolabs, Inc.) and was ligated in the forward direction into a Bstz17I (New England Biolabs, Inc) site in Fgf23 intron 2 ( ). .. The R176Q-Fgf23 ADHR mutation (from nt 528c gc 530–528c ag 530) and a silent SacI site 3′ to the R176Q mutation (from nt 627 gagct G 632–627 gagct C 632) were directed to the AatII-SbfI fragment of Fgf23 exon 3 using PCR with complementary primers containing the mutated nucleotides in the center position and the high-fidelity polymerase PfU (Agilent Technologies).

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  • 92
    New England Biolabs bstz17i
    Bstz17i, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bstz17i/product/New England Biolabs
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    bstz17i - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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