bstz17i (New England Biolabs)

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Name:

BstZ17I HF

Description:

BstZ17I HF 5 000 units

Catalog Number:

R3594L

Price:

282

Category:

Restriction Enzymes

Size:

5 000 units

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Structured Review

New England Biolabs bstz17i

BstZ17I HF 5 000 units
https://www.bioz.com/result/bstz17i/product/New England Biolabs
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99

bstz17i - by Bioz Stars, 2021-05

96/100 stars

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1) Product Images from "Edwardsiellosis Caused by Edwardsiella ictaluri in Laboratory Populations of Zebrafish Danio rerio"

Article Title: Edwardsiellosis Caused by Edwardsiella ictaluri in Laboratory Populations of Zebrafish Danio rerio

Journal: Journal of aquatic animal health

doi: 10.1080/08997659.2013.782226

Linearized plasmid profiles of Edwardsiella ictaluri isolates. Plasmid DNA from E. ictaluri isolated from Channel Catfish or Zebrafish was digested with EcoRI or BstZ17I, respectively, and separated by 0.6% agarose gel electrophoresis using a 1-kb DNA

Figure Legend Snippet: Linearized plasmid profiles of Edwardsiella ictaluri isolates. Plasmid DNA from E. ictaluri isolated from Channel Catfish or Zebrafish was digested with EcoRI or BstZ17I, respectively, and separated by 0.6% agarose gel electrophoresis using a 1-kb DNA

Techniques Used: Plasmid Preparation, Isolation, Agarose Gel Electrophoresis

Agarose Gel Electrophoresis:

Article Title: Edwardsiellosis Caused by Edwardsiella ictaluri in Laboratory Populations of Zebrafish Danio rerio
Article Snippet: Supercoiled plasmids were separated by 0.6% agarose gel electrophoresis with supercoiled ladder (New England Biolabs., Ipswich, Massachusetts) as the size standard. .. Plasmids were digested with either EcoRI or BstZ17I and were separated by 0.6% agarose gel electrophoresis with 1-kb ladder (New England Biolabs) as the size standard. .. Bacterial cultures were grown to late log phase and were pelleted by centrifugation, followed by two washes with PBS at pH 7.4.

Construct:

Article Title: Aedes cadherin mediates the in vivo toxicity of the Cry11Aa toxin to Aedes aegypti
Article Snippet: All PCR products were cloned into the pCR2.1 vector (Invitrogen, Grand Island, NY) and fully sequenced (Institute for Integrative Genome Biology, University of California, Riverside, CA). .. To construct the modified AaeCad, the 5EM and AaeCad were each digested with NotI and Bstz17I (New England Biolabs, Ipswich, MA), the fragments then gel-purified and ligated. .. Next, this construct and the 3EM were digested with BlpI and SacI, gel-purified, and ligated.

Modification:

Plasmid Preparation:

Article Title: Iron deficiency drives an autosomal dominant hypophosphatemic rickets (ADHR) phenotype in fibroblast growth factor-23 (Fgf23) knock-in mice
Article Snippet: This Fgf23 129-strain clone (a generous gift of Beate Lanske, Harvard University, Cambridge, MA) was used to make an Fgf23-null mouse ( ) and was subcloned into pBluescript(SK+) (pBSK). .. The neomycin resistance gene ( Neor ) flanked by LoxP sites was removed from the pBSK-PGKneoLox2 vector (the generous gift of Fred Dick, University of Ottawa, Ottawa) using an EcoRV-SmaI digest (New England Biolabs, Inc.) and was ligated in the forward direction into a Bstz17I (New England Biolabs, Inc) site in Fgf23 intron 2 ( ). .. The R176Q-Fgf23 ADHR mutation (from nt 528c gc 530–528c ag 530) and a silent SacI site 3′ to the R176Q mutation (from nt 627 gagct G 632–627 gagct C 632) were directed to the AatII-SbfI fragment of Fgf23 exon 3 using PCR with complementary primers containing the mutated nucleotides in the center position and the high-fidelity polymerase PfU (Agilent Technologies).

Article Title: Chemogenetic ON and OFF switches for RNA virus replication
Article Snippet: VSV-DsRed-ΔP (recombinant VSV Indiana strain lacking the viral envelope protein P) was generated by exchange of P with DsRed via pDsRed-Express-N1 (Clontech) . .. In brief, a VSV Indiana plasmid was digested with BstZ17I and MluI. .. C-terminal N after BstZ17I and the intergenic region after N were PCR amplified by primer pair XhoI-N-for/EcoRI-N-rev and digested with XhoI and EcoRI.