mfe i hf  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Name:
    MfeI HF
    Description:
    MfeI HF 2 500 units
    Catalog Number:
    r3589l
    Price:
    302
    Size:
    2 500 units
    Category:
    Restriction Enzymes
    Buy from Supplier


    Structured Review

    New England Biolabs mfe i hf
    MfeI HF
    MfeI HF 2 500 units
    https://www.bioz.com/result/mfe i hf/product/New England Biolabs
    Average 94 stars, based on 3393 article reviews
    Price from $9.99 to $1999.99
    mfe i hf - by Bioz Stars, 2020-09
    94/100 stars

    Images

    1) Product Images from "Biallelic Gene Targeting in Rice 1Biallelic Gene Targeting in Rice 1 [OPEN]"

    Article Title: Biallelic Gene Targeting in Rice 1Biallelic Gene Targeting in Rice 1 [OPEN]

    Journal: Plant Physiology

    doi: 10.1104/pp.15.01663

    -tolerant calli. A, Schematic representation of PCR- Mfe tolerance and two silent mutations (T− > A at +1479, C− > T at +1545) are marked by blue and red vertical lines, respectively. The W548L and S627I mutations create novel Mfe -22) used for PCR, and the expected size of PCR-amplified fragments and their Mfe -tolerant calli by PCR- Mfe locus. Heteroduplex of mutated and nonmutated amplicons generated by PCR reaction is partially tolerant to Mfe I-HF and imperfect digested products at W548L appeared as an approximately 700-bp fragment (*).
    Figure Legend Snippet: -tolerant calli. A, Schematic representation of PCR- Mfe tolerance and two silent mutations (T− > A at +1479, C− > T at +1545) are marked by blue and red vertical lines, respectively. The W548L and S627I mutations create novel Mfe -22) used for PCR, and the expected size of PCR-amplified fragments and their Mfe -tolerant calli by PCR- Mfe locus. Heteroduplex of mutated and nonmutated amplicons generated by PCR reaction is partially tolerant to Mfe I-HF and imperfect digested products at W548L appeared as an approximately 700-bp fragment (*).

    Techniques Used: Polymerase Chain Reaction, Amplification, Generated

    locus. A, Diagram showing the location of probes and expected band size in Southern-blot analysis using probes A and B. B, Southern-blot analysis of Mfe gene with the W548L mutation. The two bands other than 11.8 kb and 4.8 kb are due to nonspecific hybridization, since these additional bands are also seen in the wild-type lane. C, Southern-blot analysis of Mfe locus. Abbreviations: LB, left border; RB, right border; M , Mfe I.
    Figure Legend Snippet: locus. A, Diagram showing the location of probes and expected band size in Southern-blot analysis using probes A and B. B, Southern-blot analysis of Mfe gene with the W548L mutation. The two bands other than 11.8 kb and 4.8 kb are due to nonspecific hybridization, since these additional bands are also seen in the wild-type lane. C, Southern-blot analysis of Mfe locus. Abbreviations: LB, left border; RB, right border; M , Mfe I.

    Techniques Used: Southern Blot, Mutagenesis, Hybridization

    2) Product Images from "A Structural Basis for BRD2/4-Mediated Host Chromatin Interaction and Oligomer Assembly of Kaposi Sarcoma-Associated Herpesvirus and Murine Gammaherpesvirus LANA ProteinsMolecular Basis for Oligomeric-DNA Binding and Episome Maintenance by KSHV LANACrystal Structure of the Gamma-2 Herpesvirus LANA DNA Binding Domain Identifies Charged Surface Residues Which Impact Viral Latency"

    Article Title: A Structural Basis for BRD2/4-Mediated Host Chromatin Interaction and Oligomer Assembly of Kaposi Sarcoma-Associated Herpesvirus and Murine Gammaherpesvirus LANA ProteinsMolecular Basis for Oligomeric-DNA Binding and Episome Maintenance by KSHV LANACrystal Structure of the Gamma-2 Herpesvirus LANA DNA Binding Domain Identifies Charged Surface Residues Which Impact Viral Latency

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1003640

    Oligomerization of the KSHV and MHV-68 LANA CTDs. A : Oligomeric assemblies of kLANA (top) and mLANA (below) CTD dimers as found in the respective crystals. Inter-chain contact areas within and between dimers are indicated. B : Details on the oligomerization sites of kLANA (left) and mLANA CTDs (right), viewed from the center of the ring (kLANA, monoclinic crystal) and the top of the linear chain (mLANA). Color scheme corresponds to Figure 1A . C : Flow profiles (black graph) and molecular weight (red graph) of kLANA(1013-1149) (top) and mLANA(124-260) (bottom) in asymmetric field flow fractionation. D : Oligomerization assay with kLANA mutants. Top left: Western blot detecting FL kLANA wt or mutants bound to GST-fused kLANA wt or mutant CTDs. Bottom left: Ponceau S –stained WB membrane showing GST-LANA(934–1162) used in this assay. (e.v.) empty vector, (-) GST only, (MUT) mutant GST-LANA CTD always corresponding to the FL LANA mutant indicated above. Right: Expression of FL LANA proteins in eukaryotic cells. The aberrant running behavior of some mutants might be due to different posttranslational modification. E : EMSA with LBS1+2 oligonucleotide and GST-LANA(934-1162) oligomerization deficient mutants. (wt+comp.) control with 10× excess of unlabeled probe. Right: Expression of GST-LANA CTD proteins; Coomassie stained SDS PAGE gel. F : Transient replication assay with oligomerization mutants and pGTR4 vector in HeLa cells. Panel I: Southern blot of replicated DNA, remaining after digest with MfeI and DpnI. Panel II: Southern blot of input DNA linearized with MfeI; pEGFP does not replicate and serves as an internal control. Assay was performed in duplicates. Panel III: LANA expression. Panel IV: Actin loading control. (-) empty vector control.
    Figure Legend Snippet: Oligomerization of the KSHV and MHV-68 LANA CTDs. A : Oligomeric assemblies of kLANA (top) and mLANA (below) CTD dimers as found in the respective crystals. Inter-chain contact areas within and between dimers are indicated. B : Details on the oligomerization sites of kLANA (left) and mLANA CTDs (right), viewed from the center of the ring (kLANA, monoclinic crystal) and the top of the linear chain (mLANA). Color scheme corresponds to Figure 1A . C : Flow profiles (black graph) and molecular weight (red graph) of kLANA(1013-1149) (top) and mLANA(124-260) (bottom) in asymmetric field flow fractionation. D : Oligomerization assay with kLANA mutants. Top left: Western blot detecting FL kLANA wt or mutants bound to GST-fused kLANA wt or mutant CTDs. Bottom left: Ponceau S –stained WB membrane showing GST-LANA(934–1162) used in this assay. (e.v.) empty vector, (-) GST only, (MUT) mutant GST-LANA CTD always corresponding to the FL LANA mutant indicated above. Right: Expression of FL LANA proteins in eukaryotic cells. The aberrant running behavior of some mutants might be due to different posttranslational modification. E : EMSA with LBS1+2 oligonucleotide and GST-LANA(934-1162) oligomerization deficient mutants. (wt+comp.) control with 10× excess of unlabeled probe. Right: Expression of GST-LANA CTD proteins; Coomassie stained SDS PAGE gel. F : Transient replication assay with oligomerization mutants and pGTR4 vector in HeLa cells. Panel I: Southern blot of replicated DNA, remaining after digest with MfeI and DpnI. Panel II: Southern blot of input DNA linearized with MfeI; pEGFP does not replicate and serves as an internal control. Assay was performed in duplicates. Panel III: LANA expression. Panel IV: Actin loading control. (-) empty vector control.

    Techniques Used: Flow Cytometry, Molecular Weight, Field Flow Fractionation, Western Blot, Mutagenesis, Staining, Plasmid Preparation, Expressing, Modification, SDS Page, Southern Blot, Control Assay

    3) Product Images from "Multiple Pairwise Analysis of Non-homologous Centromere Coupling Reveals Preferential Chromosome Size-Dependent Interactions and a Role for Bouquet Formation in Establishing the Interaction Pattern"

    Article Title: Multiple Pairwise Analysis of Non-homologous Centromere Coupling Reveals Preferential Chromosome Size-Dependent Interactions and a Role for Bouquet Formation in Establishing the Interaction Pattern

    Journal: PLoS Genetics

    doi: 10.1371/journal.pgen.1006347

    3C2D-qPCR design for characterizing centromere coupling. (A) Design of two primers (arrow) and one Taqman probe (ball-and-stick) to quantify the interaction between restriction fragments ligated together, each encompassing a non-homologous centromere (oval). (B) Distribution of restriction enzyme sites on fragments encompassing the centromere ( CEN ) on all 16 chromosomes using an EcoRI single digestion (3C) (left) or an EcoRI-MfeI double digestion (3C2D) (right). For each chromosome (on y-axis), the distances of the restriction sites delimitating the CEN fragment are given in kilobases (kb), in relation to the center of the CEN (x-axis). Blue vertical lines indicate EcoRI sites and red lines indicate MfeI sites.
    Figure Legend Snippet: 3C2D-qPCR design for characterizing centromere coupling. (A) Design of two primers (arrow) and one Taqman probe (ball-and-stick) to quantify the interaction between restriction fragments ligated together, each encompassing a non-homologous centromere (oval). (B) Distribution of restriction enzyme sites on fragments encompassing the centromere ( CEN ) on all 16 chromosomes using an EcoRI single digestion (3C) (left) or an EcoRI-MfeI double digestion (3C2D) (right). For each chromosome (on y-axis), the distances of the restriction sites delimitating the CEN fragment are given in kilobases (kb), in relation to the center of the CEN (x-axis). Blue vertical lines indicate EcoRI sites and red lines indicate MfeI sites.

    Techniques Used: Real-time Polymerase Chain Reaction

    Related Articles

    Southern Blot:

    Article Title: Nuclear pores as versatile reference standards for quantitative superresolution microscopy
    Article Snippet: .. Southern blotting was performed in accordance Koch et al. Genomic DNA was prepared using the Wizard Genomic DNA Purification kit (Promega) and digested with SspI-HF and MfeI-HF (NEB). ..

    Multiple Displacement Amplification:

    Article Title: Chromatin remodeling by the CHD7 protein is impaired by mutations that cause human developmental disorders
    Article Snippet: .. Here, we measured the ability of remodeling factors to expose an MfeI restriction site at +28-bp inside the nucleosomes and used 1.5 U/μL of MfeI-HF (New England Biolabs). hSWI/SNF complex concentrations are given assuming a Molecular Mass of ∼1.2 MDa). .. Data were quantified using the ImageQuantTL (GE Healthcare Life Sciences) and cut DNA intensity was normalized to cytosine content before calculating cut/uncut ratios and plotting the data using GraphPad Prism.

    Purification:

    Article Title: Rapid identification and expression of human TCRs in retrogenic mice
    Article Snippet: .. Purified PCR products from the beta nested reaction and the TCR-pMIA vector were digested with Bst bI (NEB #R0519S) and Mfe I (NEB #R3589L). .. The digested PCR products were purified using a DNA purification kit (Zymo Research #11-305C).

    Generated:

    Article Title: Multiple Pairwise Analysis of Non-homologous Centromere Coupling Reveals Preferential Chromosome Size-Dependent Interactions and a Role for Bouquet Formation in Establishing the Interaction Pattern
    Article Snippet: .. For the double digestion, EcoRI-HF and MfeI-HF (NEB) generated cohesive ends that were compatible, yet recognizing slightly different sequences (GAATTC and CAATTG respectively). .. They were selected due to their extremely low star activity, extended enzymatic half-life suitable for overnight digestion and stronger activity in samples of lower purity, making them appropriate for digestion of crosslinked chromatin.

    DNA Purification:

    Article Title: Nuclear pores as versatile reference standards for quantitative superresolution microscopy
    Article Snippet: .. Southern blotting was performed in accordance Koch et al. Genomic DNA was prepared using the Wizard Genomic DNA Purification kit (Promega) and digested with SspI-HF and MfeI-HF (NEB). ..

    Polymerase Chain Reaction:

    Article Title: Rapid identification and expression of human TCRs in retrogenic mice
    Article Snippet: .. Purified PCR products from the beta nested reaction and the TCR-pMIA vector were digested with Bst bI (NEB #R0519S) and Mfe I (NEB #R3589L). .. The digested PCR products were purified using a DNA purification kit (Zymo Research #11-305C).

    Article Title: Biallelic Gene Targeting in Rice 1Biallelic Gene Targeting in Rice 1 [OPEN]
    Article Snippet: .. To confirm the occurrence of in -tolerant rice plants, we performed PCR analysis coupled with Mfe I digestion using Mfe I-HF; a high fidelity version of Mfe I was supplied by New England Biolabs (Ipswich, MA). .. PCR products were subjected to direct sequence analysis or cloned into pCR-Blunt II-TOPO (Invitrogen, San Diego, CA) and then subjected to sequencing analysis using an ABI3130 sequencer (Applied Biosystems, Foster City, CA).

    Plasmid Preparation:

    Article Title: Rapid identification and expression of human TCRs in retrogenic mice
    Article Snippet: .. Purified PCR products from the beta nested reaction and the TCR-pMIA vector were digested with Bst bI (NEB #R0519S) and Mfe I (NEB #R3589L). .. The digested PCR products were purified using a DNA purification kit (Zymo Research #11-305C).

    Article Title: Mafb lineage tracing to distinguish macrophages from other immune lineages reveals dual identity of Langerhans cells
    Article Snippet: .. Second, the intermediate pENTR plasmid was digested using MfeI-HF (New England Biolabs, Inc.) and BamHI, and then was ligated to a Pgk1 -NeoR fragment released from pENTR lox-FRT-rNeo using EcoRI and BamHI. .. Orientation of the synthetic fragment in the intermediate pENTR plasmid determined orientation of the Pgk1 -NeoR fragment in the final product, yielding either pENTR FRT-Neo or pENTR FRT-rNeo.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    New England Biolabs mfe i hf
    -tolerant calli. A, Schematic representation of <t>PCR-</t> Mfe tolerance and two silent mutations (T− > A at +1479, C− > T at +1545) are marked by blue and red vertical lines, respectively. The W548L and S627I mutations create novel Mfe -22) used for PCR, and the expected size of PCR-amplified fragments and their Mfe -tolerant calli by PCR- Mfe locus. Heteroduplex of mutated and nonmutated amplicons generated by PCR reaction is partially tolerant to Mfe I-HF and imperfect digested products at W548L appeared as an approximately 700-bp fragment (*).
    Mfe I Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mfe i hf/product/New England Biolabs
    Average 94 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    mfe i hf - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    -tolerant calli. A, Schematic representation of PCR- Mfe tolerance and two silent mutations (T− > A at +1479, C− > T at +1545) are marked by blue and red vertical lines, respectively. The W548L and S627I mutations create novel Mfe -22) used for PCR, and the expected size of PCR-amplified fragments and their Mfe -tolerant calli by PCR- Mfe locus. Heteroduplex of mutated and nonmutated amplicons generated by PCR reaction is partially tolerant to Mfe I-HF and imperfect digested products at W548L appeared as an approximately 700-bp fragment (*).

    Journal: Plant Physiology

    Article Title: Biallelic Gene Targeting in Rice 1Biallelic Gene Targeting in Rice 1 [OPEN]

    doi: 10.1104/pp.15.01663

    Figure Lengend Snippet: -tolerant calli. A, Schematic representation of PCR- Mfe tolerance and two silent mutations (T− > A at +1479, C− > T at +1545) are marked by blue and red vertical lines, respectively. The W548L and S627I mutations create novel Mfe -22) used for PCR, and the expected size of PCR-amplified fragments and their Mfe -tolerant calli by PCR- Mfe locus. Heteroduplex of mutated and nonmutated amplicons generated by PCR reaction is partially tolerant to Mfe I-HF and imperfect digested products at W548L appeared as an approximately 700-bp fragment (*).

    Article Snippet: To confirm the occurrence of in -tolerant rice plants, we performed PCR analysis coupled with Mfe I digestion using Mfe I-HF; a high fidelity version of Mfe I was supplied by New England Biolabs (Ipswich, MA).

    Techniques: Polymerase Chain Reaction, Amplification, Generated

    locus. A, Diagram showing the location of probes and expected band size in Southern-blot analysis using probes A and B. B, Southern-blot analysis of Mfe gene with the W548L mutation. The two bands other than 11.8 kb and 4.8 kb are due to nonspecific hybridization, since these additional bands are also seen in the wild-type lane. C, Southern-blot analysis of Mfe locus. Abbreviations: LB, left border; RB, right border; M , Mfe I.

    Journal: Plant Physiology

    Article Title: Biallelic Gene Targeting in Rice 1Biallelic Gene Targeting in Rice 1 [OPEN]

    doi: 10.1104/pp.15.01663

    Figure Lengend Snippet: locus. A, Diagram showing the location of probes and expected band size in Southern-blot analysis using probes A and B. B, Southern-blot analysis of Mfe gene with the W548L mutation. The two bands other than 11.8 kb and 4.8 kb are due to nonspecific hybridization, since these additional bands are also seen in the wild-type lane. C, Southern-blot analysis of Mfe locus. Abbreviations: LB, left border; RB, right border; M , Mfe I.

    Article Snippet: To confirm the occurrence of in -tolerant rice plants, we performed PCR analysis coupled with Mfe I digestion using Mfe I-HF; a high fidelity version of Mfe I was supplied by New England Biolabs (Ipswich, MA).

    Techniques: Southern Blot, Mutagenesis, Hybridization

    Oligomerization of the KSHV and MHV-68 LANA CTDs. A : Oligomeric assemblies of kLANA (top) and mLANA (below) CTD dimers as found in the respective crystals. Inter-chain contact areas within and between dimers are indicated. B : Details on the oligomerization sites of kLANA (left) and mLANA CTDs (right), viewed from the center of the ring (kLANA, monoclinic crystal) and the top of the linear chain (mLANA). Color scheme corresponds to Figure 1A . C : Flow profiles (black graph) and molecular weight (red graph) of kLANA(1013-1149) (top) and mLANA(124-260) (bottom) in asymmetric field flow fractionation. D : Oligomerization assay with kLANA mutants. Top left: Western blot detecting FL kLANA wt or mutants bound to GST-fused kLANA wt or mutant CTDs. Bottom left: Ponceau S –stained WB membrane showing GST-LANA(934–1162) used in this assay. (e.v.) empty vector, (-) GST only, (MUT) mutant GST-LANA CTD always corresponding to the FL LANA mutant indicated above. Right: Expression of FL LANA proteins in eukaryotic cells. The aberrant running behavior of some mutants might be due to different posttranslational modification. E : EMSA with LBS1+2 oligonucleotide and GST-LANA(934-1162) oligomerization deficient mutants. (wt+comp.) control with 10× excess of unlabeled probe. Right: Expression of GST-LANA CTD proteins; Coomassie stained SDS PAGE gel. F : Transient replication assay with oligomerization mutants and pGTR4 vector in HeLa cells. Panel I: Southern blot of replicated DNA, remaining after digest with MfeI and DpnI. Panel II: Southern blot of input DNA linearized with MfeI; pEGFP does not replicate and serves as an internal control. Assay was performed in duplicates. Panel III: LANA expression. Panel IV: Actin loading control. (-) empty vector control.

    Journal: PLoS Pathogens

    Article Title: A Structural Basis for BRD2/4-Mediated Host Chromatin Interaction and Oligomer Assembly of Kaposi Sarcoma-Associated Herpesvirus and Murine Gammaherpesvirus LANA ProteinsMolecular Basis for Oligomeric-DNA Binding and Episome Maintenance by KSHV LANACrystal Structure of the Gamma-2 Herpesvirus LANA DNA Binding Domain Identifies Charged Surface Residues Which Impact Viral Latency

    doi: 10.1371/journal.ppat.1003640

    Figure Lengend Snippet: Oligomerization of the KSHV and MHV-68 LANA CTDs. A : Oligomeric assemblies of kLANA (top) and mLANA (below) CTD dimers as found in the respective crystals. Inter-chain contact areas within and between dimers are indicated. B : Details on the oligomerization sites of kLANA (left) and mLANA CTDs (right), viewed from the center of the ring (kLANA, monoclinic crystal) and the top of the linear chain (mLANA). Color scheme corresponds to Figure 1A . C : Flow profiles (black graph) and molecular weight (red graph) of kLANA(1013-1149) (top) and mLANA(124-260) (bottom) in asymmetric field flow fractionation. D : Oligomerization assay with kLANA mutants. Top left: Western blot detecting FL kLANA wt or mutants bound to GST-fused kLANA wt or mutant CTDs. Bottom left: Ponceau S –stained WB membrane showing GST-LANA(934–1162) used in this assay. (e.v.) empty vector, (-) GST only, (MUT) mutant GST-LANA CTD always corresponding to the FL LANA mutant indicated above. Right: Expression of FL LANA proteins in eukaryotic cells. The aberrant running behavior of some mutants might be due to different posttranslational modification. E : EMSA with LBS1+2 oligonucleotide and GST-LANA(934-1162) oligomerization deficient mutants. (wt+comp.) control with 10× excess of unlabeled probe. Right: Expression of GST-LANA CTD proteins; Coomassie stained SDS PAGE gel. F : Transient replication assay with oligomerization mutants and pGTR4 vector in HeLa cells. Panel I: Southern blot of replicated DNA, remaining after digest with MfeI and DpnI. Panel II: Southern blot of input DNA linearized with MfeI; pEGFP does not replicate and serves as an internal control. Assay was performed in duplicates. Panel III: LANA expression. Panel IV: Actin loading control. (-) empty vector control.

    Article Snippet: 90% of DNA was digested for 72 h with 60 U of MfeI HF (or KpnI) (NEB) and 60 U DpnI (NEB) and the remaining 10% with 40 U MfeI HF (or KpnI) only.

    Techniques: Flow Cytometry, Molecular Weight, Field Flow Fractionation, Western Blot, Mutagenesis, Staining, Plasmid Preparation, Expressing, Modification, SDS Page, Southern Blot, Control Assay

    3C2D-qPCR design for characterizing centromere coupling. (A) Design of two primers (arrow) and one Taqman probe (ball-and-stick) to quantify the interaction between restriction fragments ligated together, each encompassing a non-homologous centromere (oval). (B) Distribution of restriction enzyme sites on fragments encompassing the centromere ( CEN ) on all 16 chromosomes using an EcoRI single digestion (3C) (left) or an EcoRI-MfeI double digestion (3C2D) (right). For each chromosome (on y-axis), the distances of the restriction sites delimitating the CEN fragment are given in kilobases (kb), in relation to the center of the CEN (x-axis). Blue vertical lines indicate EcoRI sites and red lines indicate MfeI sites.

    Journal: PLoS Genetics

    Article Title: Multiple Pairwise Analysis of Non-homologous Centromere Coupling Reveals Preferential Chromosome Size-Dependent Interactions and a Role for Bouquet Formation in Establishing the Interaction Pattern

    doi: 10.1371/journal.pgen.1006347

    Figure Lengend Snippet: 3C2D-qPCR design for characterizing centromere coupling. (A) Design of two primers (arrow) and one Taqman probe (ball-and-stick) to quantify the interaction between restriction fragments ligated together, each encompassing a non-homologous centromere (oval). (B) Distribution of restriction enzyme sites on fragments encompassing the centromere ( CEN ) on all 16 chromosomes using an EcoRI single digestion (3C) (left) or an EcoRI-MfeI double digestion (3C2D) (right). For each chromosome (on y-axis), the distances of the restriction sites delimitating the CEN fragment are given in kilobases (kb), in relation to the center of the CEN (x-axis). Blue vertical lines indicate EcoRI sites and red lines indicate MfeI sites.

    Article Snippet: For the double digestion, EcoRI-HF and MfeI-HF (NEB) generated cohesive ends that were compatible, yet recognizing slightly different sequences (GAATTC and CAATTG respectively).

    Techniques: Real-time Polymerase Chain Reaction