bbsi  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Name:
    BbsI HF
    Description:
    BbsI HF 1 500 units
    Catalog Number:
    r3539l
    Price:
    282
    Category:
    Restriction Enzymes
    Size:
    1 500 units
    Buy from Supplier


    Structured Review

    New England Biolabs bbsi
    BbsI HF
    BbsI HF 1 500 units
    https://www.bioz.com/result/bbsi/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bbsi - by Bioz Stars, 2021-03
    95/100 stars

    Images

    Related Articles

    Plasmid Preparation:

    Article Title: Joint Universal Modular Plasmids (JUMP): A flexible and comprehensive platform for synthetic biology
    Article Snippet: In the first step of the two-step assembly in secondary sties, equimolar amounts of the inserts are assembled without destination vector as in conventional modular cloning assemblies. .. Meanwhile, the destination vector is digested with AarI (Thermofisher) or BbsI-HF (NEB) and Shrimp Alkaline Phosphatase (NEB). .. In the second step, equimolar amounts of insert assembly and destination vector are ligated with freshly added T4 ligase enzyme and T4 ligase buffer (NEB) for one hour at 16 °C.

    Article Title: Downregulation of KCTD12 contributes to melanoma stemness by modulating CD271
    Article Snippet: .. First, the PX458 plasmid (Micro Helix, Beijing, China), which contained an eGFP tag, was enzyme digested by the BbsI-HF restriction endonuclease (New England Biolabs, USA). .. Second, the annealed double strand sgRNA was connected to the open-loop PX458 plasmid.

    Article Title: The Biogenesis of SRP RNA Is Modulated by an RNA Folding Intermediate Attained during Transcription.
    Article Snippet: The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality.

    Polymerase Chain Reaction:

    Article Title: A Drosophila CRISPR/Cas9 Toolkit for Conditionally Manipulating Gene Expression in the Prothoracic Gland as a Test Case for Polytene Tissues
    Article Snippet: Optimal target sites were then confirmed by sequencing the loci from genomic DNA extracted from corresponding fly lines we used for plasmid injection. .. The pCFD5 and PG-gRNA plasmids were pre-digested with BbsI (NEB R3539S) and fused to appropriate gRNA-containing PCR fragments via the Gibson reaction, followed by Sanger sequencing. .. For PG2-gRNA, which only has a single BbsI cutting site, we used PG1 as a template to extend the scaffold for multiple gRNA sequences in the PG2 vector.

    Sequencing:

    Article Title: A Drosophila CRISPR/Cas9 Toolkit for Conditionally Manipulating Gene Expression in the Prothoracic Gland as a Test Case for Polytene Tissues
    Article Snippet: Optimal target sites were then confirmed by sequencing the loci from genomic DNA extracted from corresponding fly lines we used for plasmid injection. .. The pCFD5 and PG-gRNA plasmids were pre-digested with BbsI (NEB R3539S) and fused to appropriate gRNA-containing PCR fragments via the Gibson reaction, followed by Sanger sequencing. .. For PG2-gRNA, which only has a single BbsI cutting site, we used PG1 as a template to extend the scaffold for multiple gRNA sequences in the PG2 vector.

    Article Title: The Biogenesis of SRP RNA Is Modulated by an RNA Folding Intermediate Attained during Transcription.
    Article Snippet: The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality.

    Clone Assay:

    Article Title: Cancer avatars derived from genetically engineered pluripotent stem cells allow for longitudinal assessment of tumor development
    Article Snippet: Each of top and bottom oligonucleotides were phosphorylated and annealed by incubating 10 µM each of oligonucleotides, 1 x T4 DNA ligase buffer (New England Biolabs), 5U T4 polynucleotide kinase (New England Biolabs) at 37°C for 30 minutes, 95°C for five minutes and by cooling down to 25°C at 0.1°C / second using a thermocycler. .. Annealed oligonucleotides were cloned into px458 by incubating 25 ng px458, 1uM annealed oligonucleotides, 1 X CutSmart buffer (New England Biolabs), 1 mM ATP (New England Biolabs), 10U BBSI-HF (New England Biolabs) and 200U T4 ligase (New England Biolabs) at 37°C for 5 minutes, 23°C for five minutes for 30 cycles. .. Correct cloning of each sgRNA sequence was confirmed by Sanger sequencing using U6 sequencing primer: 5’-GATACAAGGCTGTTAGAGAGATAATT-3’.

    Article Title: The Biogenesis of SRP RNA Is Modulated by an RNA Folding Intermediate Attained during Transcription.
    Article Snippet: The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality.

    Construct:

    Article Title: The Biogenesis of SRP RNA Is Modulated by an RNA Folding Intermediate Attained during Transcription.
    Article Snippet: The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality.

    Variant Assay:

    Article Title: The Biogenesis of SRP RNA Is Modulated by an RNA Folding Intermediate Attained during Transcription.
    Article Snippet: The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality.

    Transformation Assay:

    Article Title: The Biogenesis of SRP RNA Is Modulated by an RNA Folding Intermediate Attained during Transcription.
    Article Snippet: The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs bbsi hf
    Bbsi Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bbsi hf/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bbsi hf - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    Image Search Results