avrii  (New England Biolabs)


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  • 99
    Name:
    BbsI HF
    Description:
    BbsI HF 1 500 units
    Catalog Number:
    R3539L
    Price:
    282
    Category:
    Restriction Enzymes
    Size:
    1 500 units
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    Structured Review

    New England Biolabs avrii
    BbsI HF
    BbsI HF 1 500 units
    https://www.bioz.com/result/avrii/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avrii - by Bioz Stars, 2021-06
    99/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: GFAP hyperpalmitoylation exacerbates astrogliosis and neurodegenerative pathology in PPT1-deficient mice
    Article Snippet: The sgRNA were synthesized by Shanghai Bioligo Biotechnology. .. The Px458 vector was digested by BbsI (New England Biolabs #R3539), annealed with gRNA and transferred to DH5α competent cells (Beijing protein Biotechnology) for transformation. ..

    Article Title: A fat-tissue sensor couples growth to oxygen availability by remotely controlling insulin secretion
    Article Snippet: The Hph construct should lead to the deletion of part of the genomic region encoding the dioxygenase domain common to all Hph isoforms, and the sima gRNAs target the region encoding that protein’s DNA-binding domain. .. The Cas9 target sequences below were synthesized as part of long oligonucleotides that were used to amplify gRNA structural sequences from pCFD6 using Q5 polymerase (New England Biolabs, #M0491S); plasmid template DNA was then removed by DpnI digestion (NEB, #R0176S). pCFD6 was linearized by BbsI digestion (NEB, #R3539S) and treated with alkaline phosphatase (NEB, #M0290S) to prevent self-ligation. .. The PCR products were recombined into the linearized, phosphatase-treated vector by Gibson assembly using the NEBuilder HiFi DNA Assembly Master Mix (NEB, #E2621S).

    Article Title: Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology
    Article Snippet: In the first step, equimolar amounts of the inserts are assembled without destination vector as in conventional modular cloning assemblies. .. Meanwhile, the destination vector is digested with AarI (Thermo Fisher Scientific Inc.) or BbsI-HF (NEB) and Shrimp Alkaline Phosphatase (NEB). .. In the second step, equimolar amounts of insert assembly and destination vector are ligated with freshly added T4 ligase enzyme and T4 ligase buffer (NEB) for one hour at 16°C.

    Article Title: Use of the CRISPR-Cas9 System in Drosophila Cultured Cells to Introduce Fluorescent Tags into Endogenous Genes.
    Article Snippet: .. The CRISPR-Cas9 system makes it possible to cause double-strand breaks in specific regions, inducing repair. .. The CRISPR-Cas9 system makes it possible to cause double-strand breaks in specific regions, inducing repair.

    Transformation Assay:

    Article Title: GFAP hyperpalmitoylation exacerbates astrogliosis and neurodegenerative pathology in PPT1-deficient mice
    Article Snippet: The sgRNA were synthesized by Shanghai Bioligo Biotechnology. .. The Px458 vector was digested by BbsI (New England Biolabs #R3539), annealed with gRNA and transferred to DH5α competent cells (Beijing protein Biotechnology) for transformation. ..

    other:

    Article Title: Use of the CRISPR-Cas9 system in Drosophila cultured cells to introduce fluorescent tags into endogenous genes
    Article Snippet: BbsI-HF enzyme (New England Biolabs, R3539).

    Synthesized:

    Article Title: A fat-tissue sensor couples growth to oxygen availability by remotely controlling insulin secretion
    Article Snippet: The Hph construct should lead to the deletion of part of the genomic region encoding the dioxygenase domain common to all Hph isoforms, and the sima gRNAs target the region encoding that protein’s DNA-binding domain. .. The Cas9 target sequences below were synthesized as part of long oligonucleotides that were used to amplify gRNA structural sequences from pCFD6 using Q5 polymerase (New England Biolabs, #M0491S); plasmid template DNA was then removed by DpnI digestion (NEB, #R0176S). pCFD6 was linearized by BbsI digestion (NEB, #R3539S) and treated with alkaline phosphatase (NEB, #M0290S) to prevent self-ligation. .. The PCR products were recombined into the linearized, phosphatase-treated vector by Gibson assembly using the NEBuilder HiFi DNA Assembly Master Mix (NEB, #E2621S).

    Expressing:

    Article Title: The Biogenesis of SRP RNA Is Modulated by an RNA Folding Intermediate Attained during Transcription.
    Article Snippet: .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality.

    Recombinant:

    Article Title: The Biogenesis of SRP RNA Is Modulated by an RNA Folding Intermediate Attained during Transcription.
    Article Snippet: .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality.

    Gel Permeation Chromatography:

    Article Title: The Biogenesis of SRP RNA Is Modulated by an RNA Folding Intermediate Attained during Transcription.
    Article Snippet: .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality.

    SYBR Green Assay:

    Article Title: The Biogenesis of SRP RNA Is Modulated by an RNA Folding Intermediate Attained during Transcription.
    Article Snippet: .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality.

    Staining:

    Article Title: The Biogenesis of SRP RNA Is Modulated by an RNA Folding Intermediate Attained during Transcription.
    Article Snippet: .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality.

    Ligation:

    Article Title: The Biogenesis of SRP RNA Is Modulated by an RNA Folding Intermediate Attained during Transcription.
    Article Snippet: .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality.

    Mutagenesis:

    Article Title: The Biogenesis of SRP RNA Is Modulated by an RNA Folding Intermediate Attained during Transcription.
    Article Snippet: .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality.

    Polymerase Chain Reaction:

    Article Title: The Biogenesis of SRP RNA Is Modulated by an RNA Folding Intermediate Attained during Transcription.
    Article Snippet: .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality.

    Purification:

    Article Title: The Biogenesis of SRP RNA Is Modulated by an RNA Folding Intermediate Attained during Transcription.
    Article Snippet: .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality.

    Lambda DNA Preparation:

    Article Title: The Biogenesis of SRP RNA Is Modulated by an RNA Folding Intermediate Attained during Transcription.
    Article Snippet: .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality.

    Polyacrylamide Gel Electrophoresis:

    Article Title: The Biogenesis of SRP RNA Is Modulated by an RNA Folding Intermediate Attained during Transcription.
    Article Snippet: .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. .. The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality.

    Incubation:

    Article Title: Use of the CRISPR-Cas9 System in Drosophila Cultured Cells to Introduce Fluorescent Tags into Endogenous Genes.
    Article Snippet: .. The CRISPR-Cas9 system makes it possible to cause double-strand breaks in specific regions, inducing repair. .. The CRISPR-Cas9 system makes it possible to cause double-strand breaks in specific regions, inducing repair.

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  • 99
    New England Biolabs bbsi hf
    JUMP design and secondary sites. ( A ) In Standard European Vector Architecture (SEVA) plasmids, three common short DNA sequences (black) flank three variable regions (colored). Variable regions are the OriV (origin of replication), AbR (antibiotic selection marker) and ‘cargo’ (any expression cassette). The invariable regions are two transcription terminators flanking the cargo (T1 and T0) and origin of conjugation (oriT). Invariable regions also contain rare cutting sites, forbidden in the sequence of variable regions. ( B ) JUMP is designed as a special cargo of SEVA vectors to allow compatibility with future OriV's and AbR's of the collection. The cargo contains the upstream modular site (with outward-facing <t>AarI</t> sites); BioBrick prefix (XbaI, EcoRI); main modular site (a screening reporter gene flanked by outwards-facing BsaI and inwards-facing BsmBI sites for level 1, and vice versa for level 2); BioBrick suffix (SpeI, PstI); and downstream modular site (with outwards-facing <t>BbsI</t> sites). SEVA's canonical SpeI site was removed to allow BioBrick compatibility. ( C ) Building constructs to test similar genes (G 1 to G n ) as sequences of interest (SOI) that depend on common auxiliary factors (Aux.) with conventional modular cloning might require multiple assembly steps per SOI: the SOI is first assembled by itself and then combined with the auxiliary elements. ( D ) Introduction of the auxiliary factors in vector chassis using orthogonal use of secondary sites reduces number of assembly steps to combine the SOI with the auxiliary factor. Squares indicate restriction sites for BsaI (blue), BsmBI (red), AarI (yellow) and BbsI (green).
    Bbsi Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bbsi hf/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bbsi hf - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

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    JUMP design and secondary sites. ( A ) In Standard European Vector Architecture (SEVA) plasmids, three common short DNA sequences (black) flank three variable regions (colored). Variable regions are the OriV (origin of replication), AbR (antibiotic selection marker) and ‘cargo’ (any expression cassette). The invariable regions are two transcription terminators flanking the cargo (T1 and T0) and origin of conjugation (oriT). Invariable regions also contain rare cutting sites, forbidden in the sequence of variable regions. ( B ) JUMP is designed as a special cargo of SEVA vectors to allow compatibility with future OriV's and AbR's of the collection. The cargo contains the upstream modular site (with outward-facing AarI sites); BioBrick prefix (XbaI, EcoRI); main modular site (a screening reporter gene flanked by outwards-facing BsaI and inwards-facing BsmBI sites for level 1, and vice versa for level 2); BioBrick suffix (SpeI, PstI); and downstream modular site (with outwards-facing BbsI sites). SEVA's canonical SpeI site was removed to allow BioBrick compatibility. ( C ) Building constructs to test similar genes (G 1 to G n ) as sequences of interest (SOI) that depend on common auxiliary factors (Aux.) with conventional modular cloning might require multiple assembly steps per SOI: the SOI is first assembled by itself and then combined with the auxiliary elements. ( D ) Introduction of the auxiliary factors in vector chassis using orthogonal use of secondary sites reduces number of assembly steps to combine the SOI with the auxiliary factor. Squares indicate restriction sites for BsaI (blue), BsmBI (red), AarI (yellow) and BbsI (green).

    Journal: Synthetic Biology

    Article Title: Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology

    doi: 10.1093/synbio/ysab003

    Figure Lengend Snippet: JUMP design and secondary sites. ( A ) In Standard European Vector Architecture (SEVA) plasmids, three common short DNA sequences (black) flank three variable regions (colored). Variable regions are the OriV (origin of replication), AbR (antibiotic selection marker) and ‘cargo’ (any expression cassette). The invariable regions are two transcription terminators flanking the cargo (T1 and T0) and origin of conjugation (oriT). Invariable regions also contain rare cutting sites, forbidden in the sequence of variable regions. ( B ) JUMP is designed as a special cargo of SEVA vectors to allow compatibility with future OriV's and AbR's of the collection. The cargo contains the upstream modular site (with outward-facing AarI sites); BioBrick prefix (XbaI, EcoRI); main modular site (a screening reporter gene flanked by outwards-facing BsaI and inwards-facing BsmBI sites for level 1, and vice versa for level 2); BioBrick suffix (SpeI, PstI); and downstream modular site (with outwards-facing BbsI sites). SEVA's canonical SpeI site was removed to allow BioBrick compatibility. ( C ) Building constructs to test similar genes (G 1 to G n ) as sequences of interest (SOI) that depend on common auxiliary factors (Aux.) with conventional modular cloning might require multiple assembly steps per SOI: the SOI is first assembled by itself and then combined with the auxiliary elements. ( D ) Introduction of the auxiliary factors in vector chassis using orthogonal use of secondary sites reduces number of assembly steps to combine the SOI with the auxiliary factor. Squares indicate restriction sites for BsaI (blue), BsmBI (red), AarI (yellow) and BbsI (green).

    Article Snippet: Meanwhile, the destination vector is digested with AarI (Thermo Fisher Scientific Inc.) or BbsI-HF (NEB) and Shrimp Alkaline Phosphatase (NEB).

    Techniques: Plasmid Preparation, Selection, Marker, Expressing, Conjugation Assay, Sequencing, Construct, Clone Assay

    Use of secondary sites with two-step assembly. ( A ) Two-step assembly works by assembling inserts first and then ligating the assembly reaction with the destination vector. Squares indicate restriction sites for BsaI (blue), BsmBI (red), AarI (yellow) and BbsI (green). ( B ) Domestication and characterization of promoters using level-0 promoter acceptor. ( C ) Domestication and characterization of terminators using level-0 terminator acceptor. T and CT terminators differ in the 5' end of the part ( Supplementary Figure S2 ), with CT terminators including a stop codon. In B and C, fluorescence was normalized to OD (600 nm) and is shown as % of that of the J23100 promoter. Error bars indicate standard deviation, n = 9 (biological and technical triplicate).

    Journal: Synthetic Biology

    Article Title: Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology

    doi: 10.1093/synbio/ysab003

    Figure Lengend Snippet: Use of secondary sites with two-step assembly. ( A ) Two-step assembly works by assembling inserts first and then ligating the assembly reaction with the destination vector. Squares indicate restriction sites for BsaI (blue), BsmBI (red), AarI (yellow) and BbsI (green). ( B ) Domestication and characterization of promoters using level-0 promoter acceptor. ( C ) Domestication and characterization of terminators using level-0 terminator acceptor. T and CT terminators differ in the 5' end of the part ( Supplementary Figure S2 ), with CT terminators including a stop codon. In B and C, fluorescence was normalized to OD (600 nm) and is shown as % of that of the J23100 promoter. Error bars indicate standard deviation, n = 9 (biological and technical triplicate).

    Article Snippet: Meanwhile, the destination vector is digested with AarI (Thermo Fisher Scientific Inc.) or BbsI-HF (NEB) and Shrimp Alkaline Phosphatase (NEB).

    Techniques: Plasmid Preparation, Fluorescence, Standard Deviation