grna sequence (New England Biolabs)

New England Biolabs is a verified supplier

New England Biolabs manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Name:

BbsI HF

Description:

BbsI HF 1 500 units

Catalog Number:

r3539l

Price:

282

Size:

1 500 units

Category:

Restriction Enzymes

Buy from Supplier

Structured Review

New England Biolabs grna sequence
BbsI HF

BbsI HF 1 500 units
https://www.bioz.com/result/grna sequence/product/New England Biolabs
Average 94 stars, based on 74 article reviews
Price from $9.99 to $1999.99

grna sequence - by Bioz Stars, 2020-07

94/100 stars

Related Products / Commonly Used Together

pspcas9 bb 2a puro
calf alkaline intestine phosphatase
pcfd6
t4 dna ligase
bbsi overhangs
alkaline phosphatase
q5 high-fidelity
dpni
bbsi
pspcas9 bb
protospacer sequence
px33034
lenticrispr v2 vector
guanidine base 5
grna sequence

Images

Polymerase Chain Reaction:

Article Title: A Drosophila CRISPR/Cas9 Toolkit for Conditionally Manipulating Gene Expression in the Prothoracic Gland as a Test Case for Polytene Tissues
Article Snippet: .. The pCFD5 and PG-gRNA plasmids were pre-digested with BbsI (NEB R3539S) and fused to appropriate gRNA-containing PCR fragments via the Gibson reaction, followed by Sanger sequencing. .. For PG2-gRNA, which only has a single BbsI cutting site, we used PG1 as a template to extend the scaffold for multiple gRNA sequences in the PG2 vector.

Sequencing:

Plasmid Preparation:

Article Title: Joint Universal Modular Plasmids (JUMP): A flexible and comprehensive platform for synthetic biology
Article Snippet: .. Meanwhile, the destination vector is digested with AarI (Thermofisher) or BbsI-HF (NEB) and Shrimp Alkaline Phosphatase (NEB). .. In the second step, equimolar amounts of insert assembly and destination vector are ligated with freshly added T4 ligase enzyme and T4 ligase buffer (NEB) for one hour at 16 °C.

Article Title: Downregulation of KCTD12 contributes to melanoma stemness by modulating CD271
Article Snippet: .. First, the PX458 plasmid (Micro Helix, Beijing, China), which contained an eGFP tag, was enzyme digested by the BbsI-HF restriction endonuclease (New England Biolabs, USA). .. Second, the annealed double strand sgRNA was connected to the open-loop PX458 plasmid.

Article Title: Dynamic nanoscale morphology of the ER surveyed by STED microscopy
Article Snippet: .. The annealed oligonucleotides were cloned into pSPCas9(BB)-2A-Puro (PX459) v2.0 (a gift from F. Zhang; Addgene plasmid 62988; ) that had been digested with BbsI-HF (R3539; New England BioLabs). .. The HDR template was generated to add a five-glycine linker and HaloTag7 sequence to the 3′ end of the NUP160 gene, fusing Halo to the C terminus of all NUP160 isoforms.

Clone Assay:

Article Title: Cancer avatars derived from genetically engineered pluripotent stem cells allow for longitudinal assessment of tumor development
Article Snippet: .. Annealed oligonucleotides were cloned into px458 by incubating 25 ng px458, 1uM annealed oligonucleotides, 1 X CutSmart buffer (New England Biolabs), 1 mM ATP (New England Biolabs), 10U BBSI-HF (New England Biolabs) and 200U T4 ligase (New England Biolabs) at 37°C for 5 minutes, 23°C for five minutes for 30 cycles. .. Correct cloning of each sgRNA sequence was confirmed by Sanger sequencing using U6 sequencing primer: 5’-GATACAAGGCTGTTAGAGAGATAATT-3’.