r3510  (New England Biolabs)


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  • 97
    Name:
    DraIII HF
    Description:
    DraIII HF 5 000 units
    Catalog Number:
    r3510l
    Price:
    282
    Size:
    5 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs r3510
    DraIII HF
    DraIII HF 5 000 units
    https://www.bioz.com/result/r3510/product/New England Biolabs
    Average 97 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    r3510 - by Bioz Stars, 2020-08
    97/100 stars

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    Related Articles

    Isolation:

    Article Title: Acetylation Mimics Within a Single Nucleosome Alter Local DNA Accessibility In Compacted Nucleosome Arrays
    Article Snippet: .. Ten mg of the purified plasmid was digested with DraIII HF enzyme (New England Biolabs, NEB), followed by gel isolation, ethanol precipitation, and resuspension of the 204 bp DC DNA fragment into 500 μl TE. .. A 2.5 kb DNA fragment containing twelve tandem repeats of a Lytechinus variegates 5S rDNA was prepared from the p12-5S-C1 plasmid as described previously .

    Incubation:

    Article Title: Acetylation Mimics Within a Single Nucleosome Alter Local DNA Accessibility In Compacted Nucleosome Arrays
    Article Snippet: .. The remaining 100 μl was warmed to 37 °C and 2 μl of DraIII HF enzyme (diluted 1:5 to 4 U/μl) was added, followed by rapid mixing and further incubation at 37 °C. ..

    Construct:

    Article Title: Synonymous nucleotide modification of the KCNH2 gene affects both mRNA characteristics and translation of the encoded hERG ion channel
    Article Snippet: .. mRNA for CD was generated using the T7 mMessage mMachine kit (catalogue no. AM1344, Ambion, Austin, TX) and purified using the MegaClear Transcription Clean Up kit (catalogue no. AM1908, ThermoFisher Scientific). pcDNA plasmid constructs for hERG-NT and hERG-CM were digested using DraIII-HF (New England Biolabs), and vectors were purified using the Monarch PCR purification kit (catalogue no. T1030S, New England Biolabs). .. 1 μg of digested plasmid was used per transcription reaction.

    Purification:

    Article Title: Synonymous nucleotide modification of the KCNH2 gene affects both mRNA characteristics and translation of the encoded hERG ion channel
    Article Snippet: .. mRNA for CD was generated using the T7 mMessage mMachine kit (catalogue no. AM1344, Ambion, Austin, TX) and purified using the MegaClear Transcription Clean Up kit (catalogue no. AM1908, ThermoFisher Scientific). pcDNA plasmid constructs for hERG-NT and hERG-CM were digested using DraIII-HF (New England Biolabs), and vectors were purified using the Monarch PCR purification kit (catalogue no. T1030S, New England Biolabs). .. 1 μg of digested plasmid was used per transcription reaction.

    Article Title: Acetylation Mimics Within a Single Nucleosome Alter Local DNA Accessibility In Compacted Nucleosome Arrays
    Article Snippet: .. Ten mg of the purified plasmid was digested with DraIII HF enzyme (New England Biolabs, NEB), followed by gel isolation, ethanol precipitation, and resuspension of the 204 bp DC DNA fragment into 500 μl TE. .. A 2.5 kb DNA fragment containing twelve tandem repeats of a Lytechinus variegates 5S rDNA was prepared from the p12-5S-C1 plasmid as described previously .

    Generated:

    Article Title: Synonymous nucleotide modification of the KCNH2 gene affects both mRNA characteristics and translation of the encoded hERG ion channel
    Article Snippet: .. mRNA for CD was generated using the T7 mMessage mMachine kit (catalogue no. AM1344, Ambion, Austin, TX) and purified using the MegaClear Transcription Clean Up kit (catalogue no. AM1908, ThermoFisher Scientific). pcDNA plasmid constructs for hERG-NT and hERG-CM were digested using DraIII-HF (New England Biolabs), and vectors were purified using the Monarch PCR purification kit (catalogue no. T1030S, New England Biolabs). .. 1 μg of digested plasmid was used per transcription reaction.

    Article Title: Chromatin Fiber Invasion and Nucleosome Displacement by the Rap1 Transcription Factor
    Article Snippet: .. About 0.33 nmol of PCR generated DNA (P3_S1, P3_S2, P3_S2∗ , P3_S1S2, P3_Rpl30 and P3_Rpl30_S1, ) was digested in 200 μl of 1 x CutSmart buffer using 100 units of BsaI-HF (NEB) and 100 units of DraIII-HF (NEB) for 8-10h at 37°C. ..

    Expressing:

    Article Title: Antibody-drug conjugate library prepared by scanning insertion of the aldehyde tag into IgG1 constant regions
    Article Snippet: .. Light and heavy chain expression vector backbone preparation Catalent plasmid pRW449 (encoding human antibody #1 kappa light chain) was used as the starting material for generating the light chain vector backbone. pRW449 was digested with BamH1-HF (NEB) and DraIII-HF (NEB) to remove the wild type human kappa light chain constant region. .. The digested plasmid DNA was purified by 1% agarose gel electrophoresis and QIAquick gel extraction kit (QIAGEN).

    Ethanol Precipitation:

    Article Title: Acetylation Mimics Within a Single Nucleosome Alter Local DNA Accessibility In Compacted Nucleosome Arrays
    Article Snippet: .. Ten mg of the purified plasmid was digested with DraIII HF enzyme (New England Biolabs, NEB), followed by gel isolation, ethanol precipitation, and resuspension of the 204 bp DC DNA fragment into 500 μl TE. .. A 2.5 kb DNA fragment containing twelve tandem repeats of a Lytechinus variegates 5S rDNA was prepared from the p12-5S-C1 plasmid as described previously .

    Polymerase Chain Reaction:

    Article Title: Synonymous nucleotide modification of the KCNH2 gene affects both mRNA characteristics and translation of the encoded hERG ion channel
    Article Snippet: .. mRNA for CD was generated using the T7 mMessage mMachine kit (catalogue no. AM1344, Ambion, Austin, TX) and purified using the MegaClear Transcription Clean Up kit (catalogue no. AM1908, ThermoFisher Scientific). pcDNA plasmid constructs for hERG-NT and hERG-CM were digested using DraIII-HF (New England Biolabs), and vectors were purified using the Monarch PCR purification kit (catalogue no. T1030S, New England Biolabs). .. 1 μg of digested plasmid was used per transcription reaction.

    Article Title: Chromatin Fiber Invasion and Nucleosome Displacement by the Rap1 Transcription Factor
    Article Snippet: .. About 0.33 nmol of PCR generated DNA (P3_S1, P3_S2, P3_S2∗ , P3_S1S2, P3_Rpl30 and P3_Rpl30_S1, ) was digested in 200 μl of 1 x CutSmart buffer using 100 units of BsaI-HF (NEB) and 100 units of DraIII-HF (NEB) for 8-10h at 37°C. ..

    Variant Assay:

    Article Title: Identification and functional analysis of missense mutations in the lecithin cholesterol acyltransferase gene in a Chilean patient with hypoalphalipoproteinemia
    Article Snippet: .. For Restriction Fragment Length Polymorphism (RFLP) analysis, we used DraIII-HF restriction enzyme (New England Biolabs, Massachusetts, USA) in order to identify c.997G > A variant (p.V333 M) and HpyCH4IV restriction enzyme (New England Biolabs, Massachusetts, USA) for detection of the c.1210A > G variant (p.M404 V). .. Visualization of restriction fragments was carried out via DNA electrophoresis on 3% agarose gels (SeaKen, Lonza, Rockland, ME, USA) with 1X SYBR Safe (Invitrogen, California, USA).

    Plasmid Preparation:

    Article Title: Synonymous nucleotide modification of the KCNH2 gene affects both mRNA characteristics and translation of the encoded hERG ion channel
    Article Snippet: .. mRNA for CD was generated using the T7 mMessage mMachine kit (catalogue no. AM1344, Ambion, Austin, TX) and purified using the MegaClear Transcription Clean Up kit (catalogue no. AM1908, ThermoFisher Scientific). pcDNA plasmid constructs for hERG-NT and hERG-CM were digested using DraIII-HF (New England Biolabs), and vectors were purified using the Monarch PCR purification kit (catalogue no. T1030S, New England Biolabs). .. 1 μg of digested plasmid was used per transcription reaction.

    Article Title: Antibody-drug conjugate library prepared by scanning insertion of the aldehyde tag into IgG1 constant regions
    Article Snippet: .. Light and heavy chain expression vector backbone preparation Catalent plasmid pRW449 (encoding human antibody #1 kappa light chain) was used as the starting material for generating the light chain vector backbone. pRW449 was digested with BamH1-HF (NEB) and DraIII-HF (NEB) to remove the wild type human kappa light chain constant region. .. The digested plasmid DNA was purified by 1% agarose gel electrophoresis and QIAquick gel extraction kit (QIAGEN).

    Article Title: Chromatin Fiber Invasion and Nucleosome Displacement by the Rap1 Transcription Factor
    Article Snippet: .. For a typical reaction, either 200 units of DraIII-HF (NEB) (for recP1P2) or 200 units of BsaI-HF (NEB) (for recP4P5) or 200 units of both DraIII-HF and BsaI-HF (NEB) (for recP1 and recP5) were added to 200 pmol of plasmid DNA in 200μl 1x NEB CutSmart buffer. .. After 8-10 h digestion at 37°C, digestion progress was analyzed by gel electrophoresis on a 1% agarose gel (run in 1 x TBE running buffer, 100 V, for 50 min) to check completeness.

    Article Title: Acetylation Mimics Within a Single Nucleosome Alter Local DNA Accessibility In Compacted Nucleosome Arrays
    Article Snippet: .. Ten mg of the purified plasmid was digested with DraIII HF enzyme (New England Biolabs, NEB), followed by gel isolation, ethanol precipitation, and resuspension of the 204 bp DC DNA fragment into 500 μl TE. .. A 2.5 kb DNA fragment containing twelve tandem repeats of a Lytechinus variegates 5S rDNA was prepared from the p12-5S-C1 plasmid as described previously .

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  • 97
    New England Biolabs draiii hf enzyme
    Draiii Hf Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/draiii hf enzyme/product/New England Biolabs
    Average 97 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    draiii hf enzyme - by Bioz Stars, 2020-08
    97/100 stars
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