eag i hf  (New England Biolabs)


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  • 95
    Name:
    EagI HF
    Description:
    EagI HF 2 500 units
    Catalog Number:
    r3505l
    Price:
    269
    Size:
    2 500 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs eag i hf
    EagI HF
    EagI HF 2 500 units
    https://www.bioz.com/result/eag i hf/product/New England Biolabs
    Average 95 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    eag i hf - by Bioz Stars, 2020-08
    95/100 stars

    Images

    1) Product Images from "Novel Multidrug-Resistant Enterococcal Mobile Linear Plasmid pELF1 Encoding vanA and vanM Gene Clusters From a Japanese Vancomycin-Resistant Enterococci Isolate"

    Article Title: Novel Multidrug-Resistant Enterococcal Mobile Linear Plasmid pELF1 Encoding vanA and vanM Gene Clusters From a Japanese Vancomycin-Resistant Enterococci Isolate

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2019.02568

    Genetic structure of vancomycin resistance, other antimicrobial drug resistance genes, and pELF1. (A) The genetic structure of vancomycin resistance genes and other antimicrobial drug resistance genes is shown. Elements IS 1216 V and IS 1542 were inserted in the Tn 1546 -like element harboring vanA gene cluster. In contrast, two IS 1216E elements were located in the same direction at both ends of the vanM gene cluster. aadE , sat4 , aphA-3 , and ermB were located in pELF1 in this order. sat4 was interrupted by IS Efm1 and IS Efa11 . (B) The schematic structure of pLFE1 is shown. The left terminal end formed a hairpin structure. On the other hand, the right end is presumed to be of the invertron type. Drug resistance genes as well as presumed replication machinery and transfer machinery genes are shown. The schematic structure was prepared using easyfig ( Sullivan et al., 2011 ). (C) Restriction map of pELF1 (143.3 kb) is shown. A single Sac I, Sal I, and Sma I site each and two Eag I sites are present in pELF1. The fragments produced by these for restriction enzymes are denoted by letters.
    Figure Legend Snippet: Genetic structure of vancomycin resistance, other antimicrobial drug resistance genes, and pELF1. (A) The genetic structure of vancomycin resistance genes and other antimicrobial drug resistance genes is shown. Elements IS 1216 V and IS 1542 were inserted in the Tn 1546 -like element harboring vanA gene cluster. In contrast, two IS 1216E elements were located in the same direction at both ends of the vanM gene cluster. aadE , sat4 , aphA-3 , and ermB were located in pELF1 in this order. sat4 was interrupted by IS Efm1 and IS Efa11 . (B) The schematic structure of pLFE1 is shown. The left terminal end formed a hairpin structure. On the other hand, the right end is presumed to be of the invertron type. Drug resistance genes as well as presumed replication machinery and transfer machinery genes are shown. The schematic structure was prepared using easyfig ( Sullivan et al., 2011 ). (C) Restriction map of pELF1 (143.3 kb) is shown. A single Sac I, Sal I, and Sma I site each and two Eag I sites are present in pELF1. The fragments produced by these for restriction enzymes are denoted by letters.

    Techniques Used: Produced

    PFGE of the restriction fragments of pELF1 with proteinase K treatment. Lanes: Low Range PFG Marker; proteinase K-treatment only; proteinase K-treated Sal I digests; proteinase K-treated Sma I digests; proteinase K-treated Eag I digests. N. D., not digested.
    Figure Legend Snippet: PFGE of the restriction fragments of pELF1 with proteinase K treatment. Lanes: Low Range PFG Marker; proteinase K-treatment only; proteinase K-treated Sal I digests; proteinase K-treated Sma I digests; proteinase K-treated Eag I digests. N. D., not digested.

    Techniques Used: Marker

    Related Articles

    Amplification:

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: .. Reactions were stopped during linear amplification, and the amplified library was then purified with the QIAquick PCR purification kit (Qiagen, Hilden, Germany), digested with BstEII-HF and EagI-HF (New England Biolab, Ipswich, MA), and purified again. .. The amplified library was cloned into pLK88, a version of pLK78 modified to include the BstEII and SphI sites. pLK88 was isolated with a QIAGEN Plasmid Plus Maxiprep kit (Qiagen, Hilden, Germany) from 200 milliliters of Escherichia coli culture.

    Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
    Article Snippet: .. Purification of Recombinant S Protein and Production of Polyclonal Rabbit Antibodies A recombinant version of S protein containing an N-terminal 6 × His-tag was obtained by amplification of the DNA region encoding S protein, excluding the start codon, with primer pair ress -F/ress -R. The purified 502 base pair PCR product and N-HisPP-pET-28a(+) ( ) were cleaved with BamHI-HF and EagI-HF restriction enzymes (New England BioLabs), re-purified, ligated, and introduced into E. coli NEB 5-alpha competent cells as described above. .. Individual bacterial colonies were passaged onto fresh solid medium and screened for the presence of N-HisPP-pET-28a(+)::ess by PCR with the primers, NHpET28-Ver-F and NHpET28-Ver-R, binding approximately 200 base pairs up- and downstream respectively form the insertion site (preparation of the DNA template for PCR was performed as described above).

    Purification:

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: .. Reactions were stopped during linear amplification, and the amplified library was then purified with the QIAquick PCR purification kit (Qiagen, Hilden, Germany), digested with BstEII-HF and EagI-HF (New England Biolab, Ipswich, MA), and purified again. .. The amplified library was cloned into pLK88, a version of pLK78 modified to include the BstEII and SphI sites. pLK88 was isolated with a QIAGEN Plasmid Plus Maxiprep kit (Qiagen, Hilden, Germany) from 200 milliliters of Escherichia coli culture.

    Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
    Article Snippet: .. Purification of Recombinant S Protein and Production of Polyclonal Rabbit Antibodies A recombinant version of S protein containing an N-terminal 6 × His-tag was obtained by amplification of the DNA region encoding S protein, excluding the start codon, with primer pair ress -F/ress -R. The purified 502 base pair PCR product and N-HisPP-pET-28a(+) ( ) were cleaved with BamHI-HF and EagI-HF restriction enzymes (New England BioLabs), re-purified, ligated, and introduced into E. coli NEB 5-alpha competent cells as described above. .. Individual bacterial colonies were passaged onto fresh solid medium and screened for the presence of N-HisPP-pET-28a(+)::ess by PCR with the primers, NHpET28-Ver-F and NHpET28-Ver-R, binding approximately 200 base pairs up- and downstream respectively form the insertion site (preparation of the DNA template for PCR was performed as described above).

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: .. 20 micrograms of plasmid was then digested by BstEII-HF and EagI-HF, treated with Shrimp Alkaline Phosphatase (New England Biolabs, Ipswich, MA), and purified with the Qiagen PCR purification kit. .. We tested two ligation reactions: 1 microgram of digested vector was ligated with either 100 nanograms or 800 nanograms of the digested insert, with 4 microliters of T4 DNA Ligase M0202M (New England Biolabs, Ipswich, MA), in an 800 or 200 microliter reaction, respectively, at room temperature for 10 minutes.

    Generated:

    Article Title: Nuclear pores as versatile reference standards for quantitative superresolution microscopy
    Article Snippet: .. Donor plasmids encoding for mMaple , SNAPf tag (NEB) and HaloTag (Promega) were generated by swapping out mEGFP using restriction enzymes EagI-HF and NheI-HF (NEB). ..

    other:

    Article Title: Generation of Full-Length Infectious cDNA Clones of Middle East Respiratory Syndrome Coronavirus.
    Article Snippet: T4 DNA ligase, Gibson Assembly Master Mix, restriction enzymes such as SfiI, AsiAI, BamHI-HF, StuI, MluI-HF, PacI and EagI-HF, and Quick-Load 1 kb Extended DNA Ladder were purchased from NEB.

    Polymerase Chain Reaction:

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: .. Reactions were stopped during linear amplification, and the amplified library was then purified with the QIAquick PCR purification kit (Qiagen, Hilden, Germany), digested with BstEII-HF and EagI-HF (New England Biolab, Ipswich, MA), and purified again. .. The amplified library was cloned into pLK88, a version of pLK78 modified to include the BstEII and SphI sites. pLK88 was isolated with a QIAGEN Plasmid Plus Maxiprep kit (Qiagen, Hilden, Germany) from 200 milliliters of Escherichia coli culture.

    Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
    Article Snippet: .. Purification of Recombinant S Protein and Production of Polyclonal Rabbit Antibodies A recombinant version of S protein containing an N-terminal 6 × His-tag was obtained by amplification of the DNA region encoding S protein, excluding the start codon, with primer pair ress -F/ress -R. The purified 502 base pair PCR product and N-HisPP-pET-28a(+) ( ) were cleaved with BamHI-HF and EagI-HF restriction enzymes (New England BioLabs), re-purified, ligated, and introduced into E. coli NEB 5-alpha competent cells as described above. .. Individual bacterial colonies were passaged onto fresh solid medium and screened for the presence of N-HisPP-pET-28a(+)::ess by PCR with the primers, NHpET28-Ver-F and NHpET28-Ver-R, binding approximately 200 base pairs up- and downstream respectively form the insertion site (preparation of the DNA template for PCR was performed as described above).

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: .. 20 micrograms of plasmid was then digested by BstEII-HF and EagI-HF, treated with Shrimp Alkaline Phosphatase (New England Biolabs, Ipswich, MA), and purified with the Qiagen PCR purification kit. .. We tested two ligation reactions: 1 microgram of digested vector was ligated with either 100 nanograms or 800 nanograms of the digested insert, with 4 microliters of T4 DNA Ligase M0202M (New England Biolabs, Ipswich, MA), in an 800 or 200 microliter reaction, respectively, at room temperature for 10 minutes.

    Staining:

    Article Title: Targeted mutagenesis in a human-parasitic nematode
    Article Snippet: .. For each sample, ~1 μg of template DNA was digested with EagI-HF (NEB, Cat. # R3505S) at 37°C for 1 h. Digested products were resolved on ~1% agarose gels stained with GelRed using 1-kb and 100-bp markers. .. Illumina sequencing of S . stercoralis Genomic DNA from populations of wild-type iL3s or Ss-unc-22- targeted F1 iL3s were collected as described above.

    Recombinant:

    Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
    Article Snippet: .. Purification of Recombinant S Protein and Production of Polyclonal Rabbit Antibodies A recombinant version of S protein containing an N-terminal 6 × His-tag was obtained by amplification of the DNA region encoding S protein, excluding the start codon, with primer pair ress -F/ress -R. The purified 502 base pair PCR product and N-HisPP-pET-28a(+) ( ) were cleaved with BamHI-HF and EagI-HF restriction enzymes (New England BioLabs), re-purified, ligated, and introduced into E. coli NEB 5-alpha competent cells as described above. .. Individual bacterial colonies were passaged onto fresh solid medium and screened for the presence of N-HisPP-pET-28a(+)::ess by PCR with the primers, NHpET28-Ver-F and NHpET28-Ver-R, binding approximately 200 base pairs up- and downstream respectively form the insertion site (preparation of the DNA template for PCR was performed as described above).

    Plasmid Preparation:

    Article Title: PHASTpep: Analysis Software for Discovery of Cell-Selective Peptides via Phage Display and Next-Generation Sequencing
    Article Snippet: .. The M13KE vector (NEB) was digested with ACC651 and EagI-HF (NEB) followed by CIP. .. Vector was purified using the Qiaquick kit (Qiagen) according to manufacturer’s instructions with a final elution into 40 uL of EB.

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: .. 20 micrograms of plasmid was then digested by BstEII-HF and EagI-HF, treated with Shrimp Alkaline Phosphatase (New England Biolabs, Ipswich, MA), and purified with the Qiagen PCR purification kit. .. We tested two ligation reactions: 1 microgram of digested vector was ligated with either 100 nanograms or 800 nanograms of the digested insert, with 4 microliters of T4 DNA Ligase M0202M (New England Biolabs, Ipswich, MA), in an 800 or 200 microliter reaction, respectively, at room temperature for 10 minutes.

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    New England Biolabs eag i hf
    Genetic structure of vancomycin resistance, other antimicrobial drug resistance genes, and pELF1. (A) The genetic structure of vancomycin resistance genes and other antimicrobial drug resistance genes is shown. Elements IS 1216 V and IS 1542 were inserted in the Tn 1546 -like element harboring vanA gene cluster. In contrast, two IS 1216E elements were located in the same direction at both ends of the vanM gene cluster. aadE , sat4 , aphA-3 , and ermB were located in pELF1 in this order. sat4 was interrupted by IS Efm1 and IS Efa11 . (B) The schematic structure of pLFE1 is shown. The left terminal end formed a hairpin structure. On the other hand, the right end is presumed to be of the invertron type. Drug resistance genes as well as presumed replication machinery and transfer machinery genes are shown. The schematic structure was prepared using easyfig ( Sullivan et al., 2011 ). (C) Restriction map of pELF1 (143.3 kb) is shown. A single Sac I, Sal I, and Sma I site each and two <t>Eag</t> I sites are present in pELF1. The fragments produced by these for restriction enzymes are denoted by letters.
    Eag I Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eag i hf/product/New England Biolabs
    Average 95 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    eag i hf - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

    Image Search Results


    Genetic structure of vancomycin resistance, other antimicrobial drug resistance genes, and pELF1. (A) The genetic structure of vancomycin resistance genes and other antimicrobial drug resistance genes is shown. Elements IS 1216 V and IS 1542 were inserted in the Tn 1546 -like element harboring vanA gene cluster. In contrast, two IS 1216E elements were located in the same direction at both ends of the vanM gene cluster. aadE , sat4 , aphA-3 , and ermB were located in pELF1 in this order. sat4 was interrupted by IS Efm1 and IS Efa11 . (B) The schematic structure of pLFE1 is shown. The left terminal end formed a hairpin structure. On the other hand, the right end is presumed to be of the invertron type. Drug resistance genes as well as presumed replication machinery and transfer machinery genes are shown. The schematic structure was prepared using easyfig ( Sullivan et al., 2011 ). (C) Restriction map of pELF1 (143.3 kb) is shown. A single Sac I, Sal I, and Sma I site each and two Eag I sites are present in pELF1. The fragments produced by these for restriction enzymes are denoted by letters.

    Journal: Frontiers in Microbiology

    Article Title: Novel Multidrug-Resistant Enterococcal Mobile Linear Plasmid pELF1 Encoding vanA and vanM Gene Clusters From a Japanese Vancomycin-Resistant Enterococci Isolate

    doi: 10.3389/fmicb.2019.02568

    Figure Lengend Snippet: Genetic structure of vancomycin resistance, other antimicrobial drug resistance genes, and pELF1. (A) The genetic structure of vancomycin resistance genes and other antimicrobial drug resistance genes is shown. Elements IS 1216 V and IS 1542 were inserted in the Tn 1546 -like element harboring vanA gene cluster. In contrast, two IS 1216E elements were located in the same direction at both ends of the vanM gene cluster. aadE , sat4 , aphA-3 , and ermB were located in pELF1 in this order. sat4 was interrupted by IS Efm1 and IS Efa11 . (B) The schematic structure of pLFE1 is shown. The left terminal end formed a hairpin structure. On the other hand, the right end is presumed to be of the invertron type. Drug resistance genes as well as presumed replication machinery and transfer machinery genes are shown. The schematic structure was prepared using easyfig ( Sullivan et al., 2011 ). (C) Restriction map of pELF1 (143.3 kb) is shown. A single Sac I, Sal I, and Sma I site each and two Eag I sites are present in pELF1. The fragments produced by these for restriction enzymes are denoted by letters.

    Article Snippet: To determine the physical map of pELF1, the excised plugs containing pELF1 DNAs were separately digested at 37°C for 24 h with 20 U of Sal I-HF (New England BioLabs, Ipswich, MA, United States), at 25°C for 24 h with 100 U of Sma I (New England BioLabs), at 37°C for 24 h with 150 U of Eag I-HF (New England BioLabs), and at 37°C for 24 h with 150 U of Sac I-HF (New England BioLabs).

    Techniques: Produced

    PFGE of the restriction fragments of pELF1 with proteinase K treatment. Lanes: Low Range PFG Marker; proteinase K-treatment only; proteinase K-treated Sal I digests; proteinase K-treated Sma I digests; proteinase K-treated Eag I digests. N. D., not digested.

    Journal: Frontiers in Microbiology

    Article Title: Novel Multidrug-Resistant Enterococcal Mobile Linear Plasmid pELF1 Encoding vanA and vanM Gene Clusters From a Japanese Vancomycin-Resistant Enterococci Isolate

    doi: 10.3389/fmicb.2019.02568

    Figure Lengend Snippet: PFGE of the restriction fragments of pELF1 with proteinase K treatment. Lanes: Low Range PFG Marker; proteinase K-treatment only; proteinase K-treated Sal I digests; proteinase K-treated Sma I digests; proteinase K-treated Eag I digests. N. D., not digested.

    Article Snippet: To determine the physical map of pELF1, the excised plugs containing pELF1 DNAs were separately digested at 37°C for 24 h with 20 U of Sal I-HF (New England BioLabs, Ipswich, MA, United States), at 25°C for 24 h with 100 U of Sma I (New England BioLabs), at 37°C for 24 h with 150 U of Eag I-HF (New England BioLabs), and at 37°C for 24 h with 150 U of Sac I-HF (New England BioLabs).

    Techniques: Marker