styi hf  (New England Biolabs)


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Bioz Manufacturer Symbol New England Biolabs manufactures this product  
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  • 94
    Name:
    StyI HF
    Description:
    StyI HF 15 000 units
    Catalog Number:
    r3500l
    Price:
    290
    Size:
    15 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs styi hf
    StyI HF
    StyI HF 15 000 units
    https://www.bioz.com/result/styi hf/product/New England Biolabs
    Average 94 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    styi hf - by Bioz Stars, 2020-09
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: The effect of ERCC1 and ERCC2 gene polymorphysims on response to cisplatin based therapy in osteosarcoma patients
    Article Snippet: .. In case of ERCC2 G312A, the PCR products of 165 bp were digested for 16 h at 37 °C by StyI-HF™ enzyme (NEB, Shanghai, China). .. StyI-HF™ enzyme cuts only in the amplified fragment of the variant allele (A) to give two bands for AA genotype (139 bp and 26 bp), single band for GG genotype (165 bp) and three bands for GA genotype (165 bp, 139 bp and 26 bp).

    Article Title: A sequence polymorphism on 8q24 is associated with survival in hepatocellular carcinoma patients who received radiation therapy
    Article Snippet: .. The 203 bp PCR products were digested by the StyI-HF restriction enzyme (New England BioLabs, USA), and a 2% agarose gel electrophoresis was conducted to identify the rs9642880 genotypes. .. The genotypes were assessed as follows: the wild-type homozygotes (GG) produced one band at 203 bp; the variant homozygotes (TT) produced two bands at 135 and 68 bp; and the heterozygotes (GT) produced three bands at 203, 135, and 68 bp (Fig. ).

    Article Title: Nonsense mutation in PMEL is associated with yellowish plumage colour phenotype in Japanese quail
    Article Snippet: .. After digestion in a 20 μl reaction mixture containing 5 μl of PCR product, 10 units of StyI-HF (New England Biolabs), and 1 × CutSmart Buffer for 6 h at 37 °C, PCR products were electrophoresed in 2% agarose gel, followed by visualization with ethidium bromide. .. Complimentary DNA synthesis, Sanger sequencing, and quantitative PCR analysis According to a previous study , in which 14-day-old whole chicken embryos were used for PMEL expression analysis, total RNA was isolated from 11-day-old whole embryos (three individuals of each strain), whose developmental stage were almost equivalent to 14-day-old chicken embryos , using TRIZOL reagent (Thermo Fisher Scientific, Waltham, MA, USA).

    Recombinant:

    Article Title: In vivo production of RNA nanostructures via programmed folding of single-stranded RNAs
    Article Snippet: .. Recombinant plasmid preparation Double-stranded DNA template and pET23a plasmid (GenScript Inc.) were double-digested by XbaI and StyI-HF restriction enzymes (New England BioLabs Inc.) by following the manufacturer-recommended protocol. ..

    Agarose Gel Electrophoresis:

    Article Title: A sequence polymorphism on 8q24 is associated with survival in hepatocellular carcinoma patients who received radiation therapy
    Article Snippet: .. The 203 bp PCR products were digested by the StyI-HF restriction enzyme (New England BioLabs, USA), and a 2% agarose gel electrophoresis was conducted to identify the rs9642880 genotypes. .. The genotypes were assessed as follows: the wild-type homozygotes (GG) produced one band at 203 bp; the variant homozygotes (TT) produced two bands at 135 and 68 bp; and the heterozygotes (GT) produced three bands at 203, 135, and 68 bp (Fig. ).

    Article Title: Nonsense mutation in PMEL is associated with yellowish plumage colour phenotype in Japanese quail
    Article Snippet: .. After digestion in a 20 μl reaction mixture containing 5 μl of PCR product, 10 units of StyI-HF (New England Biolabs), and 1 × CutSmart Buffer for 6 h at 37 °C, PCR products were electrophoresed in 2% agarose gel, followed by visualization with ethidium bromide. .. Complimentary DNA synthesis, Sanger sequencing, and quantitative PCR analysis According to a previous study , in which 14-day-old whole chicken embryos were used for PMEL expression analysis, total RNA was isolated from 11-day-old whole embryos (three individuals of each strain), whose developmental stage were almost equivalent to 14-day-old chicken embryos , using TRIZOL reagent (Thermo Fisher Scientific, Waltham, MA, USA).

    Plasmid Preparation:

    Article Title: In vivo production of RNA nanostructures via programmed folding of single-stranded RNAs
    Article Snippet: .. Recombinant plasmid preparation Double-stranded DNA template and pET23a plasmid (GenScript Inc.) were double-digested by XbaI and StyI-HF restriction enzymes (New England BioLabs Inc.) by following the manufacturer-recommended protocol. ..

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  • 94
    New England Biolabs styi hf
    Styi Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/styi hf/product/New England Biolabs
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    styi hf - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

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