styi hf  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
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  • 99
    Name:
    StyI HF
    Description:
    StyI HF 15 000 units
    Catalog Number:
    r3500l
    Price:
    290
    Size:
    15 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs styi hf
    StyI HF
    StyI HF 15 000 units
    https://www.bioz.com/result/styi hf/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    styi hf - by Bioz Stars, 2020-04
    99/100 stars

    Related Products / Commonly Used Together

    pcr
    6-base cutter
    msei

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    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Nonsense mutation in PMEL is associated with yellowish plumage colour phenotype in Japanese quail
    Article Snippet: .. After digestion in a 20 μl reaction mixture containing 5 μl of PCR product, 10 units of StyI-HF (New England Biolabs), and 1 × CutSmart Buffer for 6 h at 37 °C, PCR products were electrophoresed in 2% agarose gel, followed by visualization with ethidium bromide. .. Complimentary DNA synthesis, Sanger sequencing, and quantitative PCR analysis According to a previous study , in which 14-day-old whole chicken embryos were used for PMEL expression analysis, total RNA was isolated from 11-day-old whole embryos (three individuals of each strain), whose developmental stage were almost equivalent to 14-day-old chicken embryos , using TRIZOL reagent (Thermo Fisher Scientific, Waltham, MA, USA).

    Mutagenesis:

    Article Title: Nonsense mutation in PMEL is associated with yellowish plumage colour phenotype in Japanese quail
    Article Snippet: By using this difference, we performed PCR-RFLP analysis to determine genotypes at the site of mutation in F2 offspring. .. After digestion in a 20 μl reaction mixture containing 5 μl of PCR product, 10 units of StyI-HF (New England Biolabs), and 1 × CutSmart Buffer for 6 h at 37 °C, PCR products were electrophoresed in 2% agarose gel, followed by visualization with ethidium bromide.

    Concentration Assay:

    Article Title: Development of Transcriptomic Markers for Population Analysis Using Restriction Site Associated RNA Sequencing (RARseq)
    Article Snippet: For double digestion we used MseI and the 6-base cutter, StyI-HF (NEB, R3500S.) .. The digestion reaction steps are as following: Normalize the cleaned double stranded cDNA to the appropriate concentration (~30ng/μl.)

    Incubation:

    Article Title: In vivo production of RNA nanostructures via programmed folding of single-stranded RNAs
    Article Snippet: Recombinant plasmid preparation Double-stranded DNA template and pET23a plasmid (GenScript Inc.) were double-digested by XbaI and StyI-HF restriction enzymes (New England BioLabs Inc.) by following the manufacturer-recommended protocol. .. Then the template and plasmid were ligated by T4 DNA ligase (New England BioLabs Inc.), and were incubated overnight at 16 °C.

    Polymerase Chain Reaction:

    Article Title: Nonsense mutation in PMEL is associated with yellowish plumage colour phenotype in Japanese quail
    Article Snippet: .. After digestion in a 20 μl reaction mixture containing 5 μl of PCR product, 10 units of StyI-HF (New England Biolabs), and 1 × CutSmart Buffer for 6 h at 37 °C, PCR products were electrophoresed in 2% agarose gel, followed by visualization with ethidium bromide. .. Complimentary DNA synthesis, Sanger sequencing, and quantitative PCR analysis According to a previous study , in which 14-day-old whole chicken embryos were used for PMEL expression analysis, total RNA was isolated from 11-day-old whole embryos (three individuals of each strain), whose developmental stage were almost equivalent to 14-day-old chicken embryos , using TRIZOL reagent (Thermo Fisher Scientific, Waltham, MA, USA).

    Recombinant:

    Article Title: In vivo production of RNA nanostructures via programmed folding of single-stranded RNAs
    Article Snippet: .. Recombinant plasmid preparation Double-stranded DNA template and pET23a plasmid (GenScript Inc.) were double-digested by XbaI and StyI-HF restriction enzymes (New England BioLabs Inc.) by following the manufacturer-recommended protocol. ..

    Plasmid Preparation:

    Article Title: In vivo production of RNA nanostructures via programmed folding of single-stranded RNAs
    Article Snippet: .. Recombinant plasmid preparation Double-stranded DNA template and pET23a plasmid (GenScript Inc.) were double-digested by XbaI and StyI-HF restriction enzymes (New England BioLabs Inc.) by following the manufacturer-recommended protocol. ..

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  • 99
    New England Biolabs styi hf
    Styi Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/styi hf/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    styi hf - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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