bsteii hf  (New England Biolabs)


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  • 99
    Name:
    BstEII HF
    Description:
    BstEII HF 10 000 units
    Catalog Number:
    r3162l
    Price:
    257
    Size:
    10 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs bsteii hf
    BstEII HF
    BstEII HF 10 000 units
    https://www.bioz.com/result/bsteii hf/product/New England Biolabs
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    bsteii hf - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: From the oligonucleotide pool generated by OLS, we generated edit-directing plasmid pools via a ligation-mediated cloning scheme described below (graphically summarized in ). .. Reactions were stopped during linear amplification, and the amplified library was then purified with the QIAquick PCR purification kit (Qiagen, Hilden, Germany), digested with BstEII-HF and EagI-HF (New England Biolab, Ipswich, MA), and purified again.

    Article Title: Generation and characterization of CD1d‐specific single‐domain antibodies with distinct functional features
    Article Snippet: Agarose gel electrophoresed purified genes encoding heavy‐chain‐only immunoglobulin (~ 700 bp) were digested with Sfi I and Bst EII (catalogue nos R0123L, R3162L; New England Biolabs, Ipswich, MA) followed by cDNA VHH gene (~ 300–400 bp) extraction through agarose gel electrophoresis. .. Isolated VHH genes were subsequently ligated into the phagemid vector pUR8100 (a derivate of pHen1 with addition of an HC‐V cassette, to enable Sfi I‐ Bst EII cloning, conferring Amp‐resistance for selection, and encoding a C‐terminal Myc and His6 tag for detection (kind gift from Dr M. El Khattabi, QVQ, Utrecht, the Netherlands) and transformed into Escherichia coli TG1 for display on filamentous bacteriophage.

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: The amplified library was cloned into pLK88, a version of pLK78 modified to include the BstEII and SphI sites. pLK88 was isolated with a QIAGEN Plasmid Plus Maxiprep kit (Qiagen, Hilden, Germany) from 200 milliliters of Escherichia coli culture. .. 20 micrograms of plasmid was then digested by BstEII-HF and EagI-HF, treated with Shrimp Alkaline Phosphatase (New England Biolabs, Ipswich, MA), and purified with the Qiagen PCR purification kit.

    Article Title: Tighter αC-helix–αL16-helix interactions seem to make p38α less prone to activation by autophosphorylation than Hog1
    Article Snippet: .. Then, the DNA was cloned to the vector of interest, using the BstEII (NEB # R3162S) and BlpI (NEB # R0585S) restriction enzymes for HOG1 and the EcoRI (NEB #R3101S) restriction enzyme for p38α. .. All HOG1 molecules were expressed from the pES86 vector as N-terminal 3X-HemeAgluttinin (3XHA)-tagged proteins, as described previously [ ].

    Flow Cytometry:

    Article Title: Generation and characterization of CD1d‐specific single‐domain antibodies with distinct functional features
    Article Snippet: Serially obtained pre‐immune and immune sera were tested for the presence of anti‐C1R‐CD1d antibodies through sequential incubation of sera with C1R‐CD1d cells, rabbit‐anti‐llama sera and FITC‐labelled swine‐anti‐rabbit antibody and analysed by flow cytometry. .. Agarose gel electrophoresed purified genes encoding heavy‐chain‐only immunoglobulin (~ 700 bp) were digested with Sfi I and Bst EII (catalogue nos R0123L, R3162L; New England Biolabs, Ipswich, MA) followed by cDNA VHH gene (~ 300–400 bp) extraction through agarose gel electrophoresis.

    Amplification:

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: .. Reactions were stopped during linear amplification, and the amplified library was then purified with the QIAquick PCR purification kit (Qiagen, Hilden, Germany), digested with BstEII-HF and EagI-HF (New England Biolab, Ipswich, MA), and purified again. .. The amplified library was cloned into pLK88, a version of pLK78 modified to include the BstEII and SphI sites. pLK88 was isolated with a QIAGEN Plasmid Plus Maxiprep kit (Qiagen, Hilden, Germany) from 200 milliliters of Escherichia coli culture.

    Article Title: Generation and characterization of CD1d‐specific single‐domain antibodies with distinct functional features
    Article Snippet: For this purpose total RNA was extracted from the peripheral blood lymphocyte, transcribed into cDNA, purified and used as a template for immunoglobulin heavy‐chain‐encoding gene amplification, as described previously. .. Agarose gel electrophoresed purified genes encoding heavy‐chain‐only immunoglobulin (~ 700 bp) were digested with Sfi I and Bst EII (catalogue nos R0123L, R3162L; New England Biolabs, Ipswich, MA) followed by cDNA VHH gene (~ 300–400 bp) extraction through agarose gel electrophoresis.

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: The amplified library was cloned into pLK88, a version of pLK78 modified to include the BstEII and SphI sites. pLK88 was isolated with a QIAGEN Plasmid Plus Maxiprep kit (Qiagen, Hilden, Germany) from 200 milliliters of Escherichia coli culture. .. 20 micrograms of plasmid was then digested by BstEII-HF and EagI-HF, treated with Shrimp Alkaline Phosphatase (New England Biolabs, Ipswich, MA), and purified with the Qiagen PCR purification kit.

    Agarose Gel Electrophoresis:

    Article Title: Generation and characterization of CD1d‐specific single‐domain antibodies with distinct functional features
    Article Snippet: .. Agarose gel electrophoresed purified genes encoding heavy‐chain‐only immunoglobulin (~ 700 bp) were digested with Sfi I and Bst EII (catalogue nos R0123L, R3162L; New England Biolabs, Ipswich, MA) followed by cDNA VHH gene (~ 300–400 bp) extraction through agarose gel electrophoresis. .. Isolated VHH genes were subsequently ligated into the phagemid vector pUR8100 (a derivate of pHen1 with addition of an HC‐V cassette, to enable Sfi I‐ Bst EII cloning, conferring Amp‐resistance for selection, and encoding a C‐terminal Myc and His6 tag for detection (kind gift from Dr M. El Khattabi, QVQ, Utrecht, the Netherlands) and transformed into Escherichia coli TG1 for display on filamentous bacteriophage.

    Ligation:

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: From the oligonucleotide pool generated by OLS, we generated edit-directing plasmid pools via a ligation-mediated cloning scheme described below (graphically summarized in ). .. Reactions were stopped during linear amplification, and the amplified library was then purified with the QIAquick PCR purification kit (Qiagen, Hilden, Germany), digested with BstEII-HF and EagI-HF (New England Biolab, Ipswich, MA), and purified again.

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: From the oligonucleotide pool generated by OLS, we generated edit-directing plasmid pools via a ligation-mediated cloning scheme described below (graphically summarized in ). .. 20 micrograms of plasmid was then digested by BstEII-HF and EagI-HF, treated with Shrimp Alkaline Phosphatase (New England Biolabs, Ipswich, MA), and purified with the Qiagen PCR purification kit.

    Mutagenesis:

    Article Title: Tighter αC-helix–αL16-helix interactions seem to make p38α less prone to activation by autophosphorylation than Hog1
    Article Snippet: Paragraph title: Plasmids constructions and mutagenesis procedures ... Then, the DNA was cloned to the vector of interest, using the BstEII (NEB # R3162S) and BlpI (NEB # R0585S) restriction enzymes for HOG1 and the EcoRI (NEB #R3101S) restriction enzyme for p38α.

    Isolation:

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: Reactions were stopped during linear amplification, and the amplified library was then purified with the QIAquick PCR purification kit (Qiagen, Hilden, Germany), digested with BstEII-HF and EagI-HF (New England Biolab, Ipswich, MA), and purified again. .. The amplified library was cloned into pLK88, a version of pLK78 modified to include the BstEII and SphI sites. pLK88 was isolated with a QIAGEN Plasmid Plus Maxiprep kit (Qiagen, Hilden, Germany) from 200 milliliters of Escherichia coli culture.

    Article Title: Generation and characterization of CD1d‐specific single‐domain antibodies with distinct functional features
    Article Snippet: One week after the last immunization, a 150‐ml blood sample was collected for peripheral blood lymphocyte isolation. .. Agarose gel electrophoresed purified genes encoding heavy‐chain‐only immunoglobulin (~ 700 bp) were digested with Sfi I and Bst EII (catalogue nos R0123L, R3162L; New England Biolabs, Ipswich, MA) followed by cDNA VHH gene (~ 300–400 bp) extraction through agarose gel electrophoresis.

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: The amplified library was cloned into pLK88, a version of pLK78 modified to include the BstEII and SphI sites. pLK88 was isolated with a QIAGEN Plasmid Plus Maxiprep kit (Qiagen, Hilden, Germany) from 200 milliliters of Escherichia coli culture. .. 20 micrograms of plasmid was then digested by BstEII-HF and EagI-HF, treated with Shrimp Alkaline Phosphatase (New England Biolabs, Ipswich, MA), and purified with the Qiagen PCR purification kit.

    Synthesized:

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: Generation of barcoded edit-directing plasmid pools The single-stranded oligonucleotides were synthesized by the Oligo Library Synthesis (OLS) platform (Agilent Technologies, Santa Clara, CA) in either the Watson or Crick orientation in order to minimize each oligonucleotide’s frequency of adenine bases. .. Reactions were stopped during linear amplification, and the amplified library was then purified with the QIAquick PCR purification kit (Qiagen, Hilden, Germany), digested with BstEII-HF and EagI-HF (New England Biolab, Ipswich, MA), and purified again.

    Negative Control:

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: 20 micrograms of plasmid was then digested by BstEII-HF and EagI-HF, treated with Shrimp Alkaline Phosphatase (New England Biolabs, Ipswich, MA), and purified with the Qiagen PCR purification kit. .. Concurrently, we ran negative control ligations lacking the insert DNA.

    Introduce:

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: The amplification primers were designed to introduce an EagI cut site into the 3’ end of the amplification product ( , primers named OLS Library Amplification F and R). .. Reactions were stopped during linear amplification, and the amplified library was then purified with the QIAquick PCR purification kit (Qiagen, Hilden, Germany), digested with BstEII-HF and EagI-HF (New England Biolab, Ipswich, MA), and purified again.

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: The amplification primers were designed to introduce an EagI cut site into the 3’ end of the amplification product ( , primers named OLS Library Amplification F and R). .. 20 micrograms of plasmid was then digested by BstEII-HF and EagI-HF, treated with Shrimp Alkaline Phosphatase (New England Biolabs, Ipswich, MA), and purified with the Qiagen PCR purification kit.

    Purification:

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: .. Reactions were stopped during linear amplification, and the amplified library was then purified with the QIAquick PCR purification kit (Qiagen, Hilden, Germany), digested with BstEII-HF and EagI-HF (New England Biolab, Ipswich, MA), and purified again. .. The amplified library was cloned into pLK88, a version of pLK78 modified to include the BstEII and SphI sites. pLK88 was isolated with a QIAGEN Plasmid Plus Maxiprep kit (Qiagen, Hilden, Germany) from 200 milliliters of Escherichia coli culture.

    Article Title: Generation and characterization of CD1d‐specific single‐domain antibodies with distinct functional features
    Article Snippet: .. Agarose gel electrophoresed purified genes encoding heavy‐chain‐only immunoglobulin (~ 700 bp) were digested with Sfi I and Bst EII (catalogue nos R0123L, R3162L; New England Biolabs, Ipswich, MA) followed by cDNA VHH gene (~ 300–400 bp) extraction through agarose gel electrophoresis. .. Isolated VHH genes were subsequently ligated into the phagemid vector pUR8100 (a derivate of pHen1 with addition of an HC‐V cassette, to enable Sfi I‐ Bst EII cloning, conferring Amp‐resistance for selection, and encoding a C‐terminal Myc and His6 tag for detection (kind gift from Dr M. El Khattabi, QVQ, Utrecht, the Netherlands) and transformed into Escherichia coli TG1 for display on filamentous bacteriophage.

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: .. 20 micrograms of plasmid was then digested by BstEII-HF and EagI-HF, treated with Shrimp Alkaline Phosphatase (New England Biolabs, Ipswich, MA), and purified with the Qiagen PCR purification kit. .. We tested two ligation reactions: 1 microgram of digested vector was ligated with either 100 nanograms or 800 nanograms of the digested insert, with 4 microliters of T4 DNA Ligase M0202M (New England Biolabs, Ipswich, MA), in an 800 or 200 microliter reaction, respectively, at room temperature for 10 minutes.

    Real-time Polymerase Chain Reaction:

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: Oligonucleotides were amplified on an AriaMx real-time PCR system (Agilent Technologies, Santa Clara, CA), using the KAPA Library Amplification kit (Kapa Biosystems, Wilmington, MA). .. Reactions were stopped during linear amplification, and the amplified library was then purified with the QIAquick PCR purification kit (Qiagen, Hilden, Germany), digested with BstEII-HF and EagI-HF (New England Biolab, Ipswich, MA), and purified again.

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: Oligonucleotides were amplified on an AriaMx real-time PCR system (Agilent Technologies, Santa Clara, CA), using the KAPA Library Amplification kit (Kapa Biosystems, Wilmington, MA). .. 20 micrograms of plasmid was then digested by BstEII-HF and EagI-HF, treated with Shrimp Alkaline Phosphatase (New England Biolabs, Ipswich, MA), and purified with the Qiagen PCR purification kit.

    Polymerase Chain Reaction:

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: .. Reactions were stopped during linear amplification, and the amplified library was then purified with the QIAquick PCR purification kit (Qiagen, Hilden, Germany), digested with BstEII-HF and EagI-HF (New England Biolab, Ipswich, MA), and purified again. .. The amplified library was cloned into pLK88, a version of pLK78 modified to include the BstEII and SphI sites. pLK88 was isolated with a QIAGEN Plasmid Plus Maxiprep kit (Qiagen, Hilden, Germany) from 200 milliliters of Escherichia coli culture.

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: .. 20 micrograms of plasmid was then digested by BstEII-HF and EagI-HF, treated with Shrimp Alkaline Phosphatase (New England Biolabs, Ipswich, MA), and purified with the Qiagen PCR purification kit. .. We tested two ligation reactions: 1 microgram of digested vector was ligated with either 100 nanograms or 800 nanograms of the digested insert, with 4 microliters of T4 DNA Ligase M0202M (New England Biolabs, Ipswich, MA), in an 800 or 200 microliter reaction, respectively, at room temperature for 10 minutes.

    Incubation:

    Article Title: Generation and characterization of CD1d‐specific single‐domain antibodies with distinct functional features
    Article Snippet: Serially obtained pre‐immune and immune sera were tested for the presence of anti‐C1R‐CD1d antibodies through sequential incubation of sera with C1R‐CD1d cells, rabbit‐anti‐llama sera and FITC‐labelled swine‐anti‐rabbit antibody and analysed by flow cytometry. .. Agarose gel electrophoresed purified genes encoding heavy‐chain‐only immunoglobulin (~ 700 bp) were digested with Sfi I and Bst EII (catalogue nos R0123L, R3162L; New England Biolabs, Ipswich, MA) followed by cDNA VHH gene (~ 300–400 bp) extraction through agarose gel electrophoresis.

    Selection:

    Article Title: Generation and characterization of CD1d‐specific single‐domain antibodies with distinct functional features
    Article Snippet: Agarose gel electrophoresed purified genes encoding heavy‐chain‐only immunoglobulin (~ 700 bp) were digested with Sfi I and Bst EII (catalogue nos R0123L, R3162L; New England Biolabs, Ipswich, MA) followed by cDNA VHH gene (~ 300–400 bp) extraction through agarose gel electrophoresis. .. Isolated VHH genes were subsequently ligated into the phagemid vector pUR8100 (a derivate of pHen1 with addition of an HC‐V cassette, to enable Sfi I‐ Bst EII cloning, conferring Amp‐resistance for selection, and encoding a C‐terminal Myc and His6 tag for detection (kind gift from Dr M. El Khattabi, QVQ, Utrecht, the Netherlands) and transformed into Escherichia coli TG1 for display on filamentous bacteriophage.

    Construct:

    Article Title: Generation and characterization of CD1d‐specific single‐domain antibodies with distinct functional features
    Article Snippet: Phage libraries were constructed by QVQ, Utrecht, the Netherlands. .. Agarose gel electrophoresed purified genes encoding heavy‐chain‐only immunoglobulin (~ 700 bp) were digested with Sfi I and Bst EII (catalogue nos R0123L, R3162L; New England Biolabs, Ipswich, MA) followed by cDNA VHH gene (~ 300–400 bp) extraction through agarose gel electrophoresis.

    Modification:

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: Reactions were stopped during linear amplification, and the amplified library was then purified with the QIAquick PCR purification kit (Qiagen, Hilden, Germany), digested with BstEII-HF and EagI-HF (New England Biolab, Ipswich, MA), and purified again. .. The amplified library was cloned into pLK88, a version of pLK78 modified to include the BstEII and SphI sites. pLK88 was isolated with a QIAGEN Plasmid Plus Maxiprep kit (Qiagen, Hilden, Germany) from 200 milliliters of Escherichia coli culture.

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: The amplified library was cloned into pLK88, a version of pLK78 modified to include the BstEII and SphI sites. pLK88 was isolated with a QIAGEN Plasmid Plus Maxiprep kit (Qiagen, Hilden, Germany) from 200 milliliters of Escherichia coli culture. .. 20 micrograms of plasmid was then digested by BstEII-HF and EagI-HF, treated with Shrimp Alkaline Phosphatase (New England Biolabs, Ipswich, MA), and purified with the Qiagen PCR purification kit.

    Transformation Assay:

    Article Title: Generation and characterization of CD1d‐specific single‐domain antibodies with distinct functional features
    Article Snippet: Agarose gel electrophoresed purified genes encoding heavy‐chain‐only immunoglobulin (~ 700 bp) were digested with Sfi I and Bst EII (catalogue nos R0123L, R3162L; New England Biolabs, Ipswich, MA) followed by cDNA VHH gene (~ 300–400 bp) extraction through agarose gel electrophoresis. .. Isolated VHH genes were subsequently ligated into the phagemid vector pUR8100 (a derivate of pHen1 with addition of an HC‐V cassette, to enable Sfi I‐ Bst EII cloning, conferring Amp‐resistance for selection, and encoding a C‐terminal Myc and His6 tag for detection (kind gift from Dr M. El Khattabi, QVQ, Utrecht, the Netherlands) and transformed into Escherichia coli TG1 for display on filamentous bacteriophage.

    Cytometry:

    Article Title: Generation and characterization of CD1d‐specific single‐domain antibodies with distinct functional features
    Article Snippet: Serially obtained pre‐immune and immune sera were tested for the presence of anti‐C1R‐CD1d antibodies through sequential incubation of sera with C1R‐CD1d cells, rabbit‐anti‐llama sera and FITC‐labelled swine‐anti‐rabbit antibody and analysed by flow cytometry. .. Agarose gel electrophoresed purified genes encoding heavy‐chain‐only immunoglobulin (~ 700 bp) were digested with Sfi I and Bst EII (catalogue nos R0123L, R3162L; New England Biolabs, Ipswich, MA) followed by cDNA VHH gene (~ 300–400 bp) extraction through agarose gel electrophoresis.

    Plasmid Preparation:

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: Paragraph title: Generation of barcoded edit-directing plasmid pools ... Reactions were stopped during linear amplification, and the amplified library was then purified with the QIAquick PCR purification kit (Qiagen, Hilden, Germany), digested with BstEII-HF and EagI-HF (New England Biolab, Ipswich, MA), and purified again.

    Article Title: Generation and characterization of CD1d‐specific single‐domain antibodies with distinct functional features
    Article Snippet: Agarose gel electrophoresed purified genes encoding heavy‐chain‐only immunoglobulin (~ 700 bp) were digested with Sfi I and Bst EII (catalogue nos R0123L, R3162L; New England Biolabs, Ipswich, MA) followed by cDNA VHH gene (~ 300–400 bp) extraction through agarose gel electrophoresis. .. Isolated VHH genes were subsequently ligated into the phagemid vector pUR8100 (a derivate of pHen1 with addition of an HC‐V cassette, to enable Sfi I‐ Bst EII cloning, conferring Amp‐resistance for selection, and encoding a C‐terminal Myc and His6 tag for detection (kind gift from Dr M. El Khattabi, QVQ, Utrecht, the Netherlands) and transformed into Escherichia coli TG1 for display on filamentous bacteriophage.

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: .. 20 micrograms of plasmid was then digested by BstEII-HF and EagI-HF, treated with Shrimp Alkaline Phosphatase (New England Biolabs, Ipswich, MA), and purified with the Qiagen PCR purification kit. .. We tested two ligation reactions: 1 microgram of digested vector was ligated with either 100 nanograms or 800 nanograms of the digested insert, with 4 microliters of T4 DNA Ligase M0202M (New England Biolabs, Ipswich, MA), in an 800 or 200 microliter reaction, respectively, at room temperature for 10 minutes.

    Article Title: Tighter αC-helix–αL16-helix interactions seem to make p38α less prone to activation by autophosphorylation than Hog1
    Article Snippet: .. Then, the DNA was cloned to the vector of interest, using the BstEII (NEB # R3162S) and BlpI (NEB # R0585S) restriction enzymes for HOG1 and the EcoRI (NEB #R3101S) restriction enzyme for p38α. .. All HOG1 molecules were expressed from the pES86 vector as N-terminal 3X-HemeAgluttinin (3XHA)-tagged proteins, as described previously [ ].

    Generated:

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: From the oligonucleotide pool generated by OLS, we generated edit-directing plasmid pools via a ligation-mediated cloning scheme described below (graphically summarized in ). .. Reactions were stopped during linear amplification, and the amplified library was then purified with the QIAquick PCR purification kit (Qiagen, Hilden, Germany), digested with BstEII-HF and EagI-HF (New England Biolab, Ipswich, MA), and purified again.

    Article Title: Generation and characterization of CD1d‐specific single‐domain antibodies with distinct functional features
    Article Snippet: Agarose gel electrophoresed purified genes encoding heavy‐chain‐only immunoglobulin (~ 700 bp) were digested with Sfi I and Bst EII (catalogue nos R0123L, R3162L; New England Biolabs, Ipswich, MA) followed by cDNA VHH gene (~ 300–400 bp) extraction through agarose gel electrophoresis. .. In this way two immune phage libraries were generated containing approximately 108 colony‐forming units (CFU) each.

    Article Title: Highly parallel genome variant engineering with CRISPR/Cas9
    Article Snippet: From the oligonucleotide pool generated by OLS, we generated edit-directing plasmid pools via a ligation-mediated cloning scheme described below (graphically summarized in ). .. 20 micrograms of plasmid was then digested by BstEII-HF and EagI-HF, treated with Shrimp Alkaline Phosphatase (New England Biolabs, Ipswich, MA), and purified with the Qiagen PCR purification kit.

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  • 99
    New England Biolabs bsteii hf
    Bsteii Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsteii hf/product/New England Biolabs
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    bsteii hf - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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