kpn1 (New England Biolabs)
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New England Biolabs manufactures this product 
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Name:
KpnI HF
Description:
KpnI HF 20 000 units
Catalog Number:
r3142l
Price:
269
Category:
Restriction Enzymes
Size:
20 000 units
|
Buy from Supplier |
Structured Review
New England Biolabs
kpn1
KpnI HF 20 000 units
https://www.bioz.com/result/kpn1/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
KpnI HF 20 000 units
https://www.bioz.com/result/kpn1/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
kpn1 - by Bioz Stars,
2021-03
94/100 stars
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Related Articles
Plasmid Preparation:Article Title: A split transcriptional repressor that links protein solubility to an orthogonal genetic circuit Article Snippet: Samples yielding normalized fluorescence at least 5σ lower than that of the sample transformed with pTAC were selected, purified vectors were sequenced, and the sTetR variants were named after the last residue of the N-terminal TetR fragment followed by the letters designating the coiled-coil fused to the termini at the split site, where EK signifies IAAL-E3 and IAAL-K3 and SZ17/18 signifies SYNZIP17 and SYNZIP18. .. The TetR gene and pGEX vector backbone were PCR amplified from pcDNA6/TR (Invitrogen) and pGEX2TK-IFP1.4, respectively, and these were assembled to create a plasmid that expresses TetR using tac promoter (pTAC-TetR) via Gibson Assembly using the Gibson Assembly® Master Mix (New England Biolabs). pTAC-TetR was digested with BamHI-HF and Article Title: Hypoxia-inducible lipid droplet-associated protein inhibits adipose triglyceride lipase Article Snippet: The vector was isolated using Qiagen plasmid maxi kit (Qiagen) according to the manufacturer’s instructions. .. The EGFP fragment was then excised from the pEGFP-N2 parent vector by enzyme digestion with Article Title: Identification of the crp gene in avian Pasteurella multocida and evaluation of the effects of crp deletion on its phenotype, virulence and immunogenicity Article Snippet: The amplified DNA fragment was inserted into pQK663 derived from pYA3332 [ ] between the Nco I and BamH I digestion sites to generate pQK163, which was then transformed into the S. Typhimurium Δasd Δcrp strain for a maltose fermentation assay. .. For expression of the Crp protein, the complete P. multocida crp sequence was amplified from P. multocida 0818 chromosomal DNA using the primers pET-crp -F and pET-crp -R. The PCR fragment was then purified and digested with Article Title: Influence of promoter/enhancer region haplotypes on MGMT transcriptional regulation: a potential biomarker for human sensitivity to alkylating agents Article Snippet: The MGMT P/E region inserts were then PCR amplified with these two new primers from the identified positive pGEM-T-MGMT clones followed the PCR protocol described above. .. Both the amplicons and pGL4.11 vector were double digested by HindIII-HF and Polymerase Chain Reaction:Article Title: A split transcriptional repressor that links protein solubility to an orthogonal genetic circuit Article Snippet: Samples yielding normalized fluorescence at least 5σ lower than that of the sample transformed with pTAC were selected, purified vectors were sequenced, and the sTetR variants were named after the last residue of the N-terminal TetR fragment followed by the letters designating the coiled-coil fused to the termini at the split site, where EK signifies IAAL-E3 and IAAL-K3 and SZ17/18 signifies SYNZIP17 and SYNZIP18. .. The TetR gene and pGEX vector backbone were PCR amplified from pcDNA6/TR (Invitrogen) and pGEX2TK-IFP1.4, respectively, and these were assembled to create a plasmid that expresses TetR using tac promoter (pTAC-TetR) via Gibson Assembly using the Gibson Assembly® Master Mix (New England Biolabs). pTAC-TetR was digested with BamHI-HF and Article Title: The ter Mutation in the Rat Dnd1 Gene Initiates Gonadal Teratomas and Infertility in Both Genders Article Snippet: Genotyping To identify the ter allele by PCR, primers terFor2 5′-GTCTGGTCTTAAGTGCTTGG-′3 and terRev2 5′-TCACTGCTTCACCACAGAAC-3′ amplified a 560 bp sequence in a total volume of 25 µl containing 12.5 µl HotStarTaq Master Mix Kit (Qiagen, Hilden, Germany), 10 pmol of each primer and 1 µl template DNA (15 min at 95°C; 35 cycles: 95°C for 30 s, 54°C for 30 s, 72°C for 30 s; 72°C for 1 min). .. The PCR products were digested by adding 0.5 µl Article Title: Identification of the crp gene in avian Pasteurella multocida and evaluation of the effects of crp deletion on its phenotype, virulence and immunogenicity Article Snippet: The amplified DNA fragment was inserted into pQK663 derived from pYA3332 [ ] between the Nco I and BamH I digestion sites to generate pQK163, which was then transformed into the S. Typhimurium Δasd Δcrp strain for a maltose fermentation assay. .. For expression of the Crp protein, the complete P. multocida crp sequence was amplified from P. multocida 0818 chromosomal DNA using the primers pET-crp -F and pET-crp -R. The PCR fragment was then purified and digested with Article Title: Effective CRISPR/Cas9-mediated correction of a Fanconi anemia defect by error-prone end joining or templated repair Article Snippet: The Surveyor assay was performed following manufacturer’s instructions (Transgenomic, UK) and gene editing frequencies were calculated as described . .. In experiments with a template ssODN for Amplification:Article Title: A split transcriptional repressor that links protein solubility to an orthogonal genetic circuit Article Snippet: Samples yielding normalized fluorescence at least 5σ lower than that of the sample transformed with pTAC were selected, purified vectors were sequenced, and the sTetR variants were named after the last residue of the N-terminal TetR fragment followed by the letters designating the coiled-coil fused to the termini at the split site, where EK signifies IAAL-E3 and IAAL-K3 and SZ17/18 signifies SYNZIP17 and SYNZIP18. .. The TetR gene and pGEX vector backbone were PCR amplified from pcDNA6/TR (Invitrogen) and pGEX2TK-IFP1.4, respectively, and these were assembled to create a plasmid that expresses TetR using tac promoter (pTAC-TetR) via Gibson Assembly using the Gibson Assembly® Master Mix (New England Biolabs). pTAC-TetR was digested with BamHI-HF and Article Title: Identification of the crp gene in avian Pasteurella multocida and evaluation of the effects of crp deletion on its phenotype, virulence and immunogenicity Article Snippet: The amplified DNA fragment was inserted into pQK663 derived from pYA3332 [ ] between the Nco I and BamH I digestion sites to generate pQK163, which was then transformed into the S. Typhimurium Δasd Δcrp strain for a maltose fermentation assay. .. For expression of the Crp protein, the complete P. multocida crp sequence was amplified from P. multocida 0818 chromosomal DNA using the primers pET-crp -F and pET-crp -R. The PCR fragment was then purified and digested with Expressing:Article Title: Identification of the crp gene in avian Pasteurella multocida and evaluation of the effects of crp deletion on its phenotype, virulence and immunogenicity Article Snippet: The amplified DNA fragment was inserted into pQK663 derived from pYA3332 [ ] between the Nco I and BamH I digestion sites to generate pQK163, which was then transformed into the S. Typhimurium Δasd Δcrp strain for a maltose fermentation assay. .. For expression of the Crp protein, the complete P. multocida crp sequence was amplified from P. multocida 0818 chromosomal DNA using the primers pET-crp -F and pET-crp -R. The PCR fragment was then purified and digested with Sequencing:Article Title: Identification of the crp gene in avian Pasteurella multocida and evaluation of the effects of crp deletion on its phenotype, virulence and immunogenicity Article Snippet: The amplified DNA fragment was inserted into pQK663 derived from pYA3332 [ ] between the Nco I and BamH I digestion sites to generate pQK163, which was then transformed into the S. Typhimurium Δasd Δcrp strain for a maltose fermentation assay. .. For expression of the Crp protein, the complete P. multocida crp sequence was amplified from P. multocida 0818 chromosomal DNA using the primers pET-crp -F and pET-crp -R. The PCR fragment was then purified and digested with Positron Emission Tomography:Article Title: Identification of the crp gene in avian Pasteurella multocida and evaluation of the effects of crp deletion on its phenotype, virulence and immunogenicity Article Snippet: The amplified DNA fragment was inserted into pQK663 derived from pYA3332 [ ] between the Nco I and BamH I digestion sites to generate pQK163, which was then transformed into the S. Typhimurium Δasd Δcrp strain for a maltose fermentation assay. .. For expression of the Crp protein, the complete P. multocida crp sequence was amplified from P. multocida 0818 chromosomal DNA using the primers pET-crp -F and pET-crp -R. The PCR fragment was then purified and digested with Purification:Article Title: Identification of the crp gene in avian Pasteurella multocida and evaluation of the effects of crp deletion on its phenotype, virulence and immunogenicity Article Snippet: The amplified DNA fragment was inserted into pQK663 derived from pYA3332 [ ] between the Nco I and BamH I digestion sites to generate pQK163, which was then transformed into the S. Typhimurium Δasd Δcrp strain for a maltose fermentation assay. .. For expression of the Crp protein, the complete P. multocida crp sequence was amplified from P. multocida 0818 chromosomal DNA using the primers pET-crp -F and pET-crp -R. The PCR fragment was then purified and digested with Article Title: Influence of promoter/enhancer region haplotypes on MGMT transcriptional regulation: a potential biomarker for human sensitivity to alkylating agents Article Snippet: The MGMT P/E region inserts were then PCR amplified with these two new primers from the identified positive pGEM-T-MGMT clones followed the PCR protocol described above. .. Both the amplicons and pGL4.11 vector were double digested by HindIII-HF and Subcloning:Article Title: High-performance chemical- and light-inducible recombinases in mammalian cells and mice Article Snippet: For insertion into a N-terminal CID or LID sub-cloning vector, recombinase terminating fragments were first PCR amplified out of a full recombinase sequence template to contain a 5′ sequence to code for an Kpn I-HF restriction site and a 3′ sequence to code for an SV40 nuclear localization signal, stop codon, and an Eco RI-HF restriction site. .. A similar process was followed as before with the C-terminal CID and LID sub-cloning vectors; however, Generated:Article Title: Effective CRISPR/Cas9-mediated correction of a Fanconi anemia defect by error-prone end joining or templated repair Article Snippet: The Surveyor assay was performed following manufacturer’s instructions (Transgenomic, UK) and gene editing frequencies were calculated as described . .. In experiments with a template ssODN for Gel Extraction:Article Title: Influence of promoter/enhancer region haplotypes on MGMT transcriptional regulation: a potential biomarker for human sensitivity to alkylating agents Article Snippet: The MGMT P/E region inserts were then PCR amplified with these two new primers from the identified positive pGEM-T-MGMT clones followed the PCR protocol described above. .. Both the amplicons and pGL4.11 vector were double digested by HindIII-HF and |
