Kpn I Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "The ter Mutation in the Rat Dnd1 Gene Initiates Gonadal Teratomas and Infertility in Both Genders"
Article Title: The ter Mutation in the Rat Dnd1 Gene Initiates Gonadal Teratomas and Infertility in Both Genders
Journal: PLoS ONE
Figure Legend Snippet: Genotyping, survival and tumor progression. ( A ) Genotyping. The ter mutation disrupts a Kpn I restriction site used for genotyping of the ter allele performing PCR amplification and restriction digest. Kpn I digest (black arrows) cleaved the PCR product of 560 bp into 380 bp and 180 bp fragments, N negative control. ( B ) Kaplan-Meyer survival analysis of male and female WKY- Dnd1 ter /Ztm rats carrying heterozygous and homozygous ter alleles compared to wild type animals. ( C ) Sizes of TGCTs and OGCTs after 3, 6 and 9 weeks of age in homozygous WKY- Dnd1 ter /Ztm rats.
Techniques Used: Mutagenesis, Polymerase Chain Reaction, Amplification, Negative Control